Adaptive individualized gene pair signatures distinguishing melanoma and predicting response to immune checkpoint blockade.

Distinguishing similar cancer subtypes and predicting responses to immune checkpoint blockade (ICB) are critical for improving clinical outcomes. However, existing gene expression signatures often suffer from batch effects and poor generalizability across cohorts. To address these limitations, we propose adaptive individualized gene pair signatures (AIGPS), a robust method that adaptively quantifies gene pair reversals and selects informative features using machine learning.

Prevalence of survival without major morbidity and associated risk factors among very preterm infants: a systematic review and meta-analysis.

Introduction: Survival without major morbidity (SWMM) in very preterm infants represents a critical outcome measure in neonatal care. This systematic review evaluates both the prevalence of SWMM among infants born before 32 weeks' gestation and the associated risk factors.

Methods: We conducted a comprehensive search of PubMed, Web of Science, Embase, Cochrane Library, Scopus, CNKI, CBM, and Wanfang databases from inception through February 4, 2025.

Distinct amyloid fibril structures formed by ALS-causing SOD1 mutants G93A and D101N.

Two hundred eight genetic mutations in SOD1 have been linked to amyotrophic lateral sclerosis (ALS). Of these, the G93A and D101N variants maintain much of their physiological function, closely resembling that of wild-type SOD1, and the SOD1-G93A transgenic mouse is the most extensively used mouse line in the study of ALS. In this study, we report two cryo-EM structures of amyloid fibrils formed by G93A and D101N mutants of SOD1 protein.

Construction of a prognostic risk-scoring model based on SASP-related genes in patients with skin cutaneous melanoma.

Background: Skin cutaneous melanoma (SKCM) is a highly aggressive and deadly subtype of skin cancer. Lack of efficient biomarkers for prognosis has limited the improvement of survival outcome for patients with SKCM.

Methods: In this study, we obtained RNA-seq data from TCGA and GTEx databases, followed by identification of differential expressed genes, univariate Cox regression, and LASSO regression to identify prognostic SASP-related genes in the TCGA datasets and constructed a prognostic risk-scoring model.

Clonal divergence and genomic meltdown in prostatic pleomorphic giant cell adenocarcinoma.

Background: Pleomorphic giant cell adenocarcinoma (PGCA) of the prostate is a rare, aggressive variant characterized by multinucleated giant cells, sarcomatoid features, and resistance to conventional therapies. Despite its recognition in the WHO 2016 guidelines, the molecular drivers and clinicopathological correlates of PGCA remain poorly characterized. This study presents the first integrative clinicogenomic profiling of PGCA, revealing a novel prognostic gene signature with direct implications for diagnosis and treatment.

Effects of different neuromuscular training modalities on balance performance in older adults: a systematic review and network meta-analysis.

Background: Neuromuscular training (NMT) is widely utilized to enhance balance and reduce fall risk in older adults, yet comparative effectiveness across various modalities remains unclear. This study aimed to systematically assess and rank the effects of sensorimotor training (ST), whole-body vibration training (WBVT), neurofunctional training (NT), and balance training (BT) on balance performance in older adults.

Methods: A systematic review and network meta-analysis was conducted following PRISMA guidelines, including 49 randomized controlled trials with a total of 3,028 older adults.

The evolving imaging of rapidly improving posterior reversible encephalopathy syndrome: A case report.

Posterior reversible encephalopathy syndrome (PRES) is a rare neurological disorder with no specific clinical symptoms in the early stage; thus, early imaging identification is of great importance. A 29-year-old pregnant woman at 37 weeks experienced sudden generalized seizures accompanied by impaired consciousness. Brain computed tomography, conducted around 3 h after the onset, revealed symmetric areas of decreased density in the basal ganglia, brainstem, and suboccipital cortex, along with cerebral swelling.

Fibroblast Growth Factor 21 Promotes Vascular Smooth Muscle Cell Contractile Polarization via p38 Mitogen-Activated Protein Kinase-Promoted Serum Response Factor Phosphorylation.

Phenotypic abnormalities in vascular smooth muscle cells (VSMCs) are believed to play essential roles in the progression of vascular diseases. Here, we explored the impact of fibroblast growth factor 21 (FGF21) on the phenotypic transition of VSMCs. Our findings revealed that FGF21 expression was substantially down-regulated in both human and mouse neointimal regions.

A novel non-nad-based PARP1 inhibitor, Japoflavone B, triggered Caspase-3/GSDME-mediated pyroptosis through ROS/p38/p53 pathway in NSCLC.

Pyroptosis has gotten more and more attention, in view of its link with innate immunity and disease. Most chemotherapy drugs could cause pyroptosis through caspase-3/GSDME pathway, which reshapes our understanding about the mechanism of anticancer. In our previous study, we found that a novel flavonoid, Japoflavone B (JFB), exhibited an excellent activity in vitro against the growth of cancer cells.

TRIM65 as a key regulator of ferroptosis and glycolysis in lactate-driven renal tubular injury and diabetic kidney disease.

Recent studies have highlighted the critical role of renal tubular epithelial cell (TEC) damage in the progression of diabetic kidney disease (DKD), where lactate accumulation is closely associated with TEC injury despite unclear mechanisms. This study demonstrates that TRIM65 knockout exacerbates diabetic kidney damage, while TEC-specific overexpression of TRIM65 ameliorates injury. Mechanistically, TRIM65 suppresses ferroptosis by targeting iron-responsive element binding protein 2 (IREB2) for ubiquitin-mediated degradation while also inhibiting glycolysis through ubiquitination and degradation of pyruvate dehydrogenase kinase 4, a key glycolytic regulator.

Correction: EBV-positive diffuse large B cell lymphoma secondary to activated phosphoinositide 3 kinase δ syndrome type 1 (APDS1): a case report and literature review.

[This corrects the article DOI: 10.3389/fimmu.2025.

Population pharmacokinetic analysis of remimazolam after continuous infusion for sedation in critically ill patients.

Introduction: The aim of the present prospective study was to model the population pharmacokinetics of remimazolam after continuous infusion in critically ill patients, and to provide a guide for remimazolam administration based on simulations that were conducted.

Patients And Methods: A total of 32 critically ill patients were enrolled in this study, with 236 plasma concentration data ultimately included for modeling. Plasma concentrations of remimazolam were quantified by a validated high-performance liquid chromatography-tandem mass spectrometry method, and the data were analyzed using non-linear mixed effect modeling.

Effect of chorioamnionitis on necrotizing enterocolitis in preterm infants: a multicenter cohort study.

Background: To explore the association between maternal chorioamnionitis (CAM) exposure and necrotising enterocolitis (NEC) in very preterm infants (VPI) or very low birth weight (VLBWI).

Methods: The aim of this multicentre cohort study was to investigate the impact of maternal CAM and its different staging on VPI or VLBWI NEC in six medical centres in Shenzhen between 2022 and 2023. The primary outcome was NEC (Bell staging ≥ II) and secondary outcomes included NEC or in-hospital mortality.

Prevalence and clinical risk factors of cardiovascular disease in patients with cancer: a cross-sectional study.

Objective: This study aimed to investigate the prevalence of common cardiovascular diseases (CVDs) and their clinical risk factors among patients with cancer and to offer clinical evidence to help manage CVD within this population.

Methods: Patients with cancer who were aged ≥ 20 years were selected from the National Health and Nutrition Examination Survey (NHANES) 2015-2020. The prevalence of CVD and its components was analyzed using weighted analysis to obtain standardized prevalence.

Integrative Single-Cell Analysis Decodes Gene Expression and Chromatin Accessibility in the Developing Human Fetal Brain.

The brain is the core of the central nervous system, responsible for regulating and integrating various physiological and psychological functions. Abnormal disruptions in genes during brain development can lead to a range of neurodevelopmental disorders. In this study, we performed a systematic investigation of human fetal brain tissue from miscarriages between 8 and 17 weeks of gestation using integrated single-cell RNA sequencing (scRNA-seq) and single-cell transposase-accessible chromatin sequencing (scATAC-seq).

Ultra-sensitive detection of microRNA in intraocular fluid using optical fiber sensing technology for central nervous system lymphoma diagnosis.

MicroRNA (miRNA) in aqueous humor holds significant promise as a non-invasive biomarker of primary central nervous system lymphoma (PCNSL), enabling early diagnosis and prognosis. However, current methods for miRNA detection often suffer from limitations affecting sensitivity, specificity, and clinical applicability. This study introduces a novel black phosphorus (BP)-enhanced fiber-optic surface plasmon resonance sensor integrated with a clustered regularly interspaced short palindromic repeats (CRISPR)-Cas13a system for ultrasensitive and single-base-specific detection of PCNSL-associated miRNA in aqueous humor.

Sleep in patients with traumatic brain injury: a systematic review and meta-analysis of actigraphy studies.

Study Objectives: Sleep disturbances are common in patients with traumatic brain injury (TBI). Actigraphy can provide an objective and naturalistic assessment of sleep patterns in patients with TBI. Yet, the evidence of objective sleep-wake abnormalities using actigraphy in this clinical population remained unclear.

Microparticle Shape Reveals a Key Role in Long-Term Particulate Endovascular Stability of Transarterial Embolization Treatment.

Transarterial embolization (TAE) is a widely used interventional procedure for treating various diseased vascular conditions, including tumors and arteriovenous malformations. The success of the TAE relies heavily on the effectiveness and stability of embolic agents used to occlude target vessels. While considerable attention has been given to the material and size of embolic particles, the role of the particulate shape in endovascular stability has been less explored.

Primary renal mucosa-associated lymphoid tissue lymphoma coexisting with immune thrombocytopenia.

Background: Primary renal mucosa-associated lymphoid tissue (MALT) lymphoma is exceedingly rare. Although immune thrombocytopenia (ITP) may accompany lymphoproliferative disorders, its association with primary renal MALT lymphoma has not been previously reported.

Case Presentation: A 60-year-old male presenting with isolated thrombocytopenia was diagnosed with ITP during hematologic evaluation.

The association between depressive symptom trajectories and risk of all-cause mortality and premature death: A 10-year follow-up study in United States and England.

Background: Depression has emerged as a leading cause of disease burden and disability worldwide. This study aimed to investigate the association between depressive symptom trajectories, all-cause mortality, and premature death.

Method: We utilized the data from Health Retirement Study (HRS) and English Longitudinal Study of Aging (ELSA).

Signatures of miRNA 5-methylcytosine modification profile and potential immune-related target gene regulation in diabetic kidney disease.

Background: Diabetic kidney disease (DKD) is a major microvascular complication of diabetes, with microRNAs (miRNAs) playing a role in its pathogenesis through methylation modifications. Preliminary experiments indicated an association between 5-methylcytosine (m5C) modification and miRNA dysregulation in peripheral blood mononuclear cells (PBMCs) of DKD patients.

Methods: We employed Arraystar's small RNA modification chip to detect m5C modifications in miRNAs from PBMCs of DKD patients and predicted target genes.

Corrigendum: Clinicopathological and molecular characterization of astroblastoma.

[This corrects the article DOI: 10.3389/fnmol.2025.

Real-world data on clinical outcomes and validation of prognostic models for angioimmunoblastic T-cell lymphoma: a multicentric retrospective study in Southern China.

Background: This study aimed to elucidate the treatment outcomes and prognosis of angioimmunoblastic T-cell lymphoma (AITL) patients in a real-world setting.

Objectives: The clinical efficacy of new drug applications was evaluated, alongside the predictive accuracy of prognostic models, to inform future research.

Methods: In this study, 82.

Identification of dynamic network biomarker for erlotinib pre-resistance using single-cell differential covariance entropy.

Acquired erlotinib resistance is the primary cause of treatment failure in patients with non-small cell lung cancer (NSCLC). Most current research focuses on end-stage resistance, whereas early events leading to resistance have been largely overlooked. In this study, we developed a novel dynamic network biomarker (DNB) method called single-cell differential covariance entropy (scDCE) to identify the pre-resistance state and associated DNB genes.

Colchicine inhibits vascular calcification by suppressing inflammasome activation through the enhancement of the Sirt2-PP2Ac signaling pathway.

Colchicine (Col) is a traditional herbal medicine derived from the plant Colchicum autumnale. With the property of anti-inflammation, Col has demonstrated certain therapeutic effects in cardiovascular diseases. Vascular calcification is positively related to the morbidity and mortality of cardiovascular diseases.