[Development of core outcome set for traditional Chinese medicine interventions in diabetic peripheral neuropathy].

This study developed a core outcome set(COS) for traditional Chinese medicine(TCM) interventions in diabetic peripheral neuropathy(DPN), standardizing evaluation metrics for TCM efficacy and providing a new framework for DPN treatment and management. A systematic search was conducted across databases, including CNKI, Wanfang, and PubMed, targeting clinical trial literature published between January 1, 2013, and January 1, 2023. The search focused on extracting outcome indicators and measurement tools used in TCM treatments for DPN.

Synovial Organoids and Cell Culture Models: An Ideal Platform for Exploring Traditional Chinese Medicine in Rheumatoid Arthritis.

Rheumatoid arthritis (RA) is a chronic autoimmune disease characterized by synovial hyperplasia, inflammation, and pannus formation. Although conventional therapies are effective, they are often associated with significant side effects. Traditional Chinese medicine (TCM), known for its high safety profile and multitarget therapeutic effects, has shown unique potential in the treatment of RA.

Yanghe Pingchuan granules inhibit cellular senescence in airway smooth muscle cells to improve bronchial asthma via modulating Nrf2 acetylation.

Background: Bronchial asthma (BA) is regarded as one of the most prevalent chronic respiratory diseases worldwide. Yanghe Pingchuan Granules (YPG), a traditional Chinese medicine (TCM) compound, has been employed extensively in treating BA. However, the precise mechanism by which it exerts its therapeutic effects remains to be fully elucidated.

Presacral tumors: a retrospective analysis of 112 cases with emphasis on diagnostic challenges and surgical outcomes.

Background: Presacral tumors are rare entities with heterogeneous pathology including malignant potential. Due to nonspecific symptoms mimicking common anorectal diseases, misdiagnosis remains a major challenge that may delay treatment and worsen prognosis, particularly for malignant variants. This study analyzes diagnostic pitfalls and surgical outcomes in a large single-center cohort.

Zuoqing granules attenuate ulcerative colitis via macrophage polarization modulation: involvement of the PPAR-γ/NF-κB/STAT1 signaling axis.

Background: Although our prior clinical study demonstrated the efficacy of Zuoqing granules (ZQGs) in treating ulcerative colitis (UC), the underlying immunomodulatory mechanisms remained unclear. This study systematically investigated ZQG's therapeutic effects through macrophage polarization modulation and related signaling pathways using both and models.

Methods: , dextran sulfate sodium (DSS)-induced UC rats were divided into normal control, DSS model, and ZQG treatment groups (high/medium/low doses).

Targeting Aquaporins for Renal Fibrosis Therapy: Mechanisms and Translational Potential.

Chronic kidney disease (CKD) is a global health problem characterized by renal fibrosis (RF). Aquaporins (AQPs) are key regulators of water transport across membranes. Key members of this family (such as AQP1, AQP2, etc.

Transcriptional specialization shapes abnormal cortical morphological similarity gradients in Wilson's disease.

Background: Neuroimaging studies have revealed structural abnormalities in the brains of individuals with Wilson's disease (WD), particularly within the basal ganglia, and the associated molecular mechanisms have been elucidated. However, the structural damage in the cerebral cortex, along with its underlying biological and molecular processes, remains elusive. Here, we investigated the abnormalities in cortical morphological similarity gradients associated with WD and further unraveled their underlying transcriptional specialization.

Construction of LncRNA-miRNA-mRNA regulatory network in IgA nephropathy mice model by RNA-sequencing and bioinformatics analysis.

Background: Immunoglobulin A nephropathy (IgAN) represents the most prevalent form of primary glomerulonephritis. Long noncoding RNAs (lncRNAs) play critical roles in the initiation and progression of various diseases, including kidney disorders. However, the involvement of lncRNAs in IgAN remains underexplored.

Efficacy of Qingrehuoxue granule for bone protection in patients with active rheumatoid arthritis in China: The PRE-ERODE randomized controlled trial.

Ethnopharmacological Relevance: Qingrehuoxue (QRHX) granules, composed of 12 Chinese herbs, have been used for over 40 years to treat rheumatoid arthritis (RA) in China and show potential in protecting bone health. However, high-quality evidence on their effects is limited.

Aim Of The Study: To assess the effects of QRHX granules on bone marrow edema (BME) in active RA patients.

Single-cell transcriptomics combined with spatial transcriptomics reveals a fibrotic microenvironment in hyperuricemic nephropathy.

Background: Hyperuricemia nephropathy (HN) is a kidney disease caused by hyperuricemia, with renal fibrosis as its hallmark lesion. Renal fibrosis involves diverse cell populations and complex intercellular communication, but its cellular composition and molecular mechanisms remain unclear.

Methods: We employed single-cell RNA sequencing and spatial transcriptomics to analyze kidney tissues from HN rats, identifying cell populations, gene expression patterns, and intercellular interactions.

Global, regional, and national epidemiology of childhood epilepsy from 1990 to 2021: a systematic study based on the GBD 2021.

Background: Epilepsy is one of the most prevalent chronic neurological disorders in children, imposing a substantial global disease burden characterized by neurodevelopmental impairments, premature mortality, and heightened socioeconomic costs. This study aimed to evaluate the global incidence, mortality, and disability-adjusted life years (DALYs) associated with childhood epilepsy from 1990 to 2021, identify key risk factors, and assess differences between regions and countries, while also predicting the global disease burden associated with childhood epilepsy up to 2035.

Method: This epidemiological analysis utilized cross-sectional data from the Global Burden of Disease Study 2021 (GBD 2021) to systematically investigate childhood epilepsy trends among individuals aged 0-14 years across 204 global jurisdictions over the period 1990-2021.

Risk factor analysis and nomogram development for advanced-stage hepatic fibrosis in patients with Wilson's disease.

Purpose: To investigate the risk factors for advanced-stage hepatic fibrosis in Wilson's disease (WD), and developed a predictive nomogram to screen high risk patients with WD for early prevention and intervention.

Methods: We retrospectively analyzed clinical data from WD in The First Affiliated Hospital of Anhui University of Chinese medicine between January 2010 and December 2024. Patients were divided into advanced hepatic fibrosis and non-advanced fibrosis groups according liver stiffness measurement.

Multi-omics and experimental validation studies reveal key biomarkers of cellular senescence in diabetic retinopathy.

Objective: Diabetic retinopathy (DR) is a prevalent microvascular complication of diabetes, contributing to vision impairment and related retinal diseases. Growing evidence indicates that cellular senescence (CS) under high-glucose conditions plays a role in the pathogenesis of DR. This study aims to identify key biomarkers of CS in DR by integrating transcriptomics, single-cell sequencing data, and experimental validation, thereby offering insights for understanding the disease mechanism and developing novel therapeutic strategies.

PCBP1 modulates cellular iron homeostasis via targeting HIF-1α/HO-1 pathway and alleviates high-glucose-induced ferroptosis in HRMECs.

Diabetic retinopathy (DR), is established as one of the leading causes of blindness in adults. Recently, evidence has emerged indicating that the iron and ferroptosis may be involved in the retinal microangiopathy inherent to DR. Here, we first identified significant differential expression of ferroptosis-related genes (FRGs) in a genetic screening of patients with PDR.

BCL6 promotes the progression of high-grade serous ovarian cancer cells by inhibiting PLAAT4.

Background: B-cell lymphoma 6 (BCL6) is increasingly recognized as a driver of cancer progression; however, the precise molecular mechanisms by which BCL6 facilitates high-grade serous ovarian cancer (HGSOC) progression remain incompletely understood.

Methods: In this study, immunohistochemical (IHC) staining was used to evaluate the expression of BCL6 and PLAAT4 in HGSOC tissues and normal tissues. Cleavage under targets and tagmentation (CUT&Tag) was combined with RNA sequencing (RNA-seq) analyses to screen and identify the downstream regulatory mechanisms of BCL6.

Global burden of male smoking-induced stroke, 1990-2021, and 20-year projections: an analysis of the 2021 Global Burden of Disease Study.

Background: Stroke represents the third most prevalent cause of mortality on a global scale. It is well-documented that smoking constitutes a significant risk factor for stroke, with males accounting for a higher proportion of the global smoking population than females. The present analysis thus focused on the global burden of stroke due to smoking in males over the period 1990-2021, with projections made for future trends in stroke due to smoking in males over the next 20 years.

A novel combination therapy with Uridine and Praziquantel effectively alleviates schistosomiasis-induced hepatic fibrosis through promoting adipogenic differentiation.

Schistosomiasis-induced hepatic fibrosis, a consequence of egg-induced granulomatous lesions, remains untreated by current drugs. Therefore, the development of novel antifibrosis drugs is of paramount importance. Our previous study indicated that aberrant uridine concentrations play a pivotal role in schistosomiasis-induced hepatic fibrosis.

Er Miao San and its main components phellodendrine and atractylenolide-I exert anti-rheumatoid arthritis effects by inhibiting PAD4 and thereby reducing the formation of NETs.

Background: Er Miao San (EMS) is an important Chinese herbal formula for the treatment of damp-heat underflow syndrome, and has been clinically proven to be effective in the treatment of rheumatoid arthritis (RA). Previous studies have shown that EMS can regulate the function of T cells and dendritic cells, affect the polarisation of macrophages, and inhibit the abnormal activation and angiogenesis of fibroblast-like synoviocytes (FLSs). However, it is unclear whether the inhibitory effect of EMS on RA is related to neutrophil dysfunction.

Three-Compartment Pharmacokinetics of Inhaled and Injected Sinapine Thiocyanate Manifest Prolonged Retention and Its Therapeutics in Acute Lung Injury.

: Acute lung injury (ALI) is driven by inflammatory cascades and reactive oxygen species (ROS) generation, with the progression to severe cases markedly increasing mortality. Sinapine thiocyanate (ST), a bioactive natural compound isolated from Sinapis Semen Albae (SSA), demonstrates both anti-inflammatory and antioxidant pharmacological activities. However, no monotherapeutic formulation of ST has been developed to date.

Investigating the mechanism of anti-hepatic fibrosis action of Chiyin Heji based on network pharmacology, molecular docking and experimental validation.

To unravel the pharmacological mechanisms and validate the efficacy of Chiyin Heji (the CYP) in anti-hepatic fibrosis, we analysed its components using UHPLC-Q-Orbitrap HRMS, screened drug targets through TCMSP, and identified disease targets using databases such as Genecard, OMIM, Drugbank and TTD. The intersection of these targets was analysed using GO and KEGG for PPI network analysis and target prediction. Molecular docking was simulated using AutoDock Vina.

As a New Tumor Suppressor Gene, PID1 Activates the AMPK-mTOR Signal to Inhibit the Progression of Bladder Cancer.

Purpose: Bladder cancer is one of the ten most common cancers in the world, with a high incidence rate and mortality, and therefore a major burden on the global health care system. PID1 (Phosphotyrosine Interaction Domain 1) functions as an intracellular receptor protein for LRP1. The purpose of this study was to explore the role of PID1 in bladder cancer.

A case report of AIDS complicated by and mixed pulmonary infection identified via metagenomic next-generation sequencing.

is a Gram-positive bacterium that is rarely encountered in clinical practice. It primarily affects the digestive and nervous systems, with pulmonary infections being infrequently reported. This case report describes a patient with HIV/AIDS who developed a mixed pulmonary infection involving and .

Prognostic value of immune-inflammation and nutritional indices in advanced hepatocellular carcinoma patients receiving immunotherapy with compound Kushen injection.

Background: Advanced hepatocellular carcinoma (HCC) with ascites (AS) lacks reliable biomarkers for predicting treatment outcomes. The combined prognostic value of the systemic immune-inflammation index (SII) and prognostic nutritional index (PNI) remains underexplored for novel therapies.

Aim: To evaluate the clinical efficacy of combining intraperitoneal compound Kushen injection (CKI) with immunotherapy in patients with advanced HCC using a scoring system that combines SII and PNI.

Effect of Acupuncture on Clinical Symptoms of Patients with Intractable Facial Paralysis: A Multicentre, Randomized, Controlled Trial.

Objective: To evaluate the clinical effect and safety of acupuncture manipulation on treatment of intractable facial paralysis (IFP), and verify the practicality and precision of the Anzhong Facial Paralysis Precision Scale (Eyelid Closure Grading Scale, AFPPS-ECGS).

Methods: A multicentre, single-blind, randomized controlled trial was conducted from October 2022 to June 2024. Eighty-nine IFP participants were randomly assigned to an ordinary acupuncture group (OAG, 45 cases) and a characteristic acupuncture group (CAG, 44 cases) using a random number table method.

Efficacy of Xiaokeqing granules and lifestyle intervention in treating prediabetes mellitus considering metabolomic biomarkers: A randomised controlled trial.

Background: Prediabetes mellitus (PDM) is receiving increasing attention as a precursor to type 2 diabetes mellitus. Lifestyle and traditional Chinese medicine (TCM) interventions are effective for PDM prevention and treatment. Therefore, we conducted a preliminary investigation and an exploratory randomised controlled trial to assess the effects of a combined lifestyle and TCM intervention on PDM indicators.