BBX10 interacts with PIF1 to prevent photo-oxidation and to promote the greening process.

Seedlings emerged from the covering soil immediately undergo de-etiolation, ensuring plants switch from heterotrophic to photoautotrophic growth. This transition is essential for seedling development and survival. However, the underlying mechanism remains largely obscure.

Editing a gibberellin receptor gene improves yield and nitrogen fixation in soybean.

Soybean is an important source of oil, protein, and feed. However, its yield is far below that of major cereal crops. The green revolution increased the yield of cereal crops partially through high-density planting of lodging-resistant semi-dwarf varieties, but required more nitrogen fertilizers, posing an environmental threat.

Transcription Factor GmERFA Interacts With GmNFYA and Acts as a Negative Regulator of Seed Fatty Acid Accumulation in Soybean.

Soybean is one of the most important oilseed crops, and its seed oil content directly determines the economic value and industrial applicability worldwide. However, how soybean seed oil accumulation is regulated remains less understood. Here, through RNA-seq analysis and screening for the interacting proteins of a positive oil regulator GmNFYA, we identified an AP2/ERF-type transcription factor GmERFA, which acts as a negative regulator of oil accumulation.

The miR172a-ERF416/413 module regulates soybean seed traits.

Soybean (Glycine max) provides vegetable oils and proteins for human consumption. Its production depends on seeds and other production-related agronomic traits. How the seed traits are regulated in soybean remains largely unclear.

Soybean domestication alters rhizosphere microbial assembly and disrupts the potential bacteria-protist relationships.

Crop domestication has long been known to reshape rhizosphere microbial communities, yet research has focused disproprotionately on bacteria and fungal responses to crop domestication while neglecting protist communities. Protists, as key microbial predators regulating bacterial populations and thereby their functionalities, remain understudied in this context. Here, we investigate the influence of soybean domestication on both bacterial and protist communities, with a focus on the reorganization of ecological strategies, specifically generalists and specialists, within these microbiomes.

Phosphoenolpyruvic Carboxylase Gene GmPPC6 Negatively Regulates Root System Development Under Low Phosphorus Stress in Soybean.

Available phosphorus (P) deficiency in soil is one of the major factors limiting the growth and development of soybean. Although great efforts have been made to identify QTLs or genes related to low P stress in soybean, few studies have been reported on the root remodeling. Here, by performing genome-wide association study (GWAS) using 239 accessions and 62 124 single-nucleotide polymorphism (SNP) markers, 194 QTN regions related to seven root traits were screened, and 5 of 194 were colocated in at least three traits or environments.

B-BOX22 isoforms activate expression of MYBs and elicit blue light-induced flavonoid biosynthesis in soybean.

Soybean (Glycine max) accumulates a substantial amount of flavonoids, including anthocyanins and flavonols, which play essential roles in both plant growth and human health. While blue light promotes flavonoid biosynthesis, the regulatory circuitry governing this process remains poorly characterized. Here, we demonstrate that blue light stimulates flavonol and anthocyanin accumulation in soybean hypocotyls by inducing the B-box transcription factor GmBBX22.

GmGASA12 coordinates hormonal dynamics to enhance soybean water-soluble protein accumulation and seed size.

Water-soluble protein (WSP) content determines soybean nutritional value and processing efficiency, yet its genetic and molecular regulation remains poorly understood. Here, we identified Glycine max gibberellic acid-stimulated Arabidopsis 12 (GmGASA12), encoding a gibberellin-regulated protein, as a major quantitative trait locus (QTL) governing WSP, through genome-wide association studies across five environments. Knockout of GmGASA12 resulted in 28.

Correction: Razzaq et al. Omics and CRISPR-Cas9 Approaches for Molecular Insight, Functional Gene Analysis, and Stress Tolerance Development in Crops. 2021, , 1292.

In the original publication [...

GWAS on HTP-enabled dynamic traits unravels novel genetic architecture of salt tolerance in soybean.

Salinity is a significant factor limiting the cultivation of soybean, a globally important cash crop. However, efficient assessment and genetic dissection of soybean response to salt stress remain challenging. This study leveraged high-throughput phenotyping (HTP) and traditional physiological methods for comprehensive phenotyping of salt tolerance using 261 diverse soybean germplasms and dissected the genetic basis through GWAS.

Knocking out the caleosin-encoding gene GmCLO1 improves soybean resistance to common cutworm.

The jasmonic acid (JA) pathway is central for plant defence against herbivores, and genes related to this pathway have received increased attention. Here, we evaluated the functions of the allene oxide cyclase (AOC)-encoding gene GmAOC3 and the caleosin-encoding gene GmCLO1, which may affect JA synthesis in soybean, and explored the anti-insect mechanisms of these two genes. The overexpression of GmAOC3 increased soybean resistance to the common cutworm (CCW).

Genome-wide locus-allele comparison reveals differential evolution dynamics from annual wild to landrace and released cultivar soybeans.

Previous studies on population evolution relied primarily on allele frequency analysis using molecular markers or genome sequence segments, like selective sweeps. With the sequencing technique developed, we suggest the genome-wide locus-allele comparison to detect the genomic structure variation among populations. Its key point lies in taking SNP linkage disequilibrium block as uniform genomic marker for genome-wide gene and inter-gene regions to meet the requirement of multiple alleles in natural populations.

Integration of GWAS and transcriptome and haplotype analyses to identify QTNs and candidate genes controlling oil content in soybean seeds.

The oil content (OC) of soybean is a critical trait with important applications in the development of both food and industrial products. Consequently, enhancing OC has consistently remained a significant objective in soybean breeding programs. In this research, a four-way recombinant inbred line (FW-RIL) population containing 144 lines developed from the cross (Kenfeng14 × Kenfeng15) × (Kenfeng19 × Heinong48) was planted in ten environments to investigate the phenotypic data for OC in seeds.

An overview of cytoplasmic male sterility in .

Rapeseed (Brassica napus ) is one of the world's most important oilseed crops, supplying humans with oil products, nutritious feed for livestock, and natural resources for industrial applications. Due to immense population pressure, more seed production is needed for human consumption due to its high quality of food products. As a vital genetic resource, male sterility provides ease in hybrid seed production and heterosis breeding.

Genome-wide identification of SABATH gene family in soybean relate to salt, aluminum, chromium toxicity.

SABATH gene family in plants participates in metabolic processes such as methylation of various hormones and plays an essential role in plant response to abiotic stress. In this study, we identified and sequenced 28 SABATH genes in soybean and divided them into three groups. Genome mapping annotation suggested that tandem repeat was the cause of SABATH gene amplification in soybean.

Inactivation of GH3.5 by COP1-mediated K63-linked ubiquitination promotes seedling hypocotyl elongation.

CONSTITUTIVELY PHOTOMORPHOGENIC 1 (COP1), which was first discovered as a central repressor of photomorphogenesis in Arabidopsis, destabilizes proteins by ubiquitination in both plants and animals. However, it is unclear whether and how Arabidopsis COP1 mediates non-proteolytic ubiquitination to regulate photomorphogenesis. Here, we show that COP1-mediated lysine 63 (K63)-linked polyubiquitination inhibits the enzyme activity of GRETCHEN HAGEN 3.

Characterization of shade tolerance gene network in soybean revealed by forward integrated reverse genetic studies.

Shade tolerance is a key trait for cultivars in inter/relay-cropped soybeans in maize fields. Our previous genome-wide association study (GWAS) results on southern China soybean germplasm revealed that the shade tolerance was conferred by a complex of genes with multiple alleles. To complete our understanding of the shade tolerance gene system, GWAS with gene-allele sequences as markers (designated GASM-RTM-GWAS) was conducted in a recombinant inbred line (RIL) population between two extreme parents using the shade tolerance index (STI) and relative pith cell length (RCL) as indicators.

A wild-allele GsPP2C-51-a1 enhances tolerance to drought stress in soybean and Arabidopsis.

A wild-allele GsPP2C-51-a1 of Glyma.14g162100 was identified in SojaCSSLP5, back to wild soybean, conferring drought tolerance. Its functions were verified in transgenic hairy root soybeans and Arabidopsis under water deficit and ABA treatment.

Identification and functional validation of a new gene conferring resistance to strains SC4 and SC20 in soybean.

Soybean Mosaic Virus (SMV) poses a serious threat to soybean production, often resulting in considerable yield losses or complete crop failure, particularly if infection occurs during early growth stages. While several SMV resistance genes have been identified, the genetic basis of resistance to certain strains remains poorly understood. Among the 22 SMV strains, SC4 and SC20 are considered pathogenic in Central China.

The scaffold protein RACK1 regulates root growth and gravitropic response by recruiting PINOID to phosphorylate the auxin efflux transporter PIN-FORMED2.

The scaffolding protein RACK1 is involved in polar auxin transport and signaling. It binds to PINOID and PIN-FORMED2, enhancing their interaction and phosphorylation-dependent auxin efflux. Knocking down RACK1 genes impairs auxin-related processes such as root growth and gravitropic response.

Enhancing soybean germination and vigor under water stress: the efficacy of bio-priming with sodium carboxymethyl cellulose and gum arabic.

Seed priming can significantly enhance the tolerance of soybean against different environmental stresses by improving seed water uptake and modulating stress-response mechanisms. In particular, seed priming with sodium carboxymethylcellulose (SCMC) and gum Arabic (GA) can support seeds to withstand extreme conditions better, promoting more consistent germination and robust seedling establishment, which is crucial for achieving stable agricultural yields. The present study investigated the effects of seed priming using a combination of SCMC and GA (10% CG) on the germination, growth, and biochemical responses of six soybean varieties under drought and flooding stress conditions.

Comparative Proteomic Atlas of Two Soybean Varieties with Contrasting Seed Oil and Protein Content.

As complex quantitative traits, soybean seed oil and protein contents are governed by dynamic proteome networks that remain largely unknown. Here, we investigated the dynamic changes of the proteome during seed maturation across two soybean varieties with contrasting seed oil and protein content. Through optimizing the detectability of low-abundance proteins and utilizing library-free data-independent acquisition (directDIA) mass spectrometry, we unprecedentedly identified 7414 proteins and 3975 protein groups (PGs), substantially expanding the soybean seed proteome.