CircSHPRH inhibits malignancy progression of head and neck squamous cell carcinoma by regulating PI3K/AKT/mTOR signaling pathway.

Background: Head and neck squamous cell carcinoma (HNSCC) stands as a significant global health concern, marked by its substantial impact on both morbidity and mortality rates. Although previous studies have suggested that circular RNAs (circRNAs) may influence HNSCC progression, the underlying mechanisms remain largely unclear.

Methods: In this study, we first used quantitative real-time polymerase chain reaction (qRT-PCR) to measure the expression levels of circSHPRH in HNSCC tissues and cell lines.

The Regulatory Mechanism of MicroRNA-144-3p on Damage to Endometrial Epithelial Cells Exposed to Copper Ions In Vitro.

Objective: To investigate the differential expression of microRNA-144-3p in endometrial cells exposed to copper ions in vitro. The specific mechanism by which microRNA-144-3p is involved in Cu-induced damage to the human endometrial epithelial cells (HEECs) was explored.

Methods: HEECs were cultured in copper-containing culture medium to simulate changes in the endometrium after copper intrauterine device (Cu-IUD) implantation.

Prenatal diagnosis of cerebellar hypoplasia in fetal ultrasound using deep learning under the constraint of the anatomical structures of the cerebellum and cistern.

Objective: The objective of this retrospective study is to develop and validate an artificial intelligence model constrained by the anatomical structure of the brain with the aim of improving the accuracy of prenatal diagnosis of fetal cerebellar hypoplasia using ultrasound imaging.

Background: Fetal central nervous system dysplasia is one of the most prevalent congenital malformations, and cerebellar hypoplasia represents a significant manifestation of this anomaly. Accurate clinical diagnosis is of great importance for the purpose of prenatal screening of fetal health.

Microglial Membrane-Coated Biomimetic Nanoplatform for Enhanced Blood-Brain Barrier Penetration and Targeted Photodynamic Therapy in Orthotopic Glioblastoma.

Glioblastoma multiforme (GBM) continues to pose a significant challenge in the field of neuro-oncology primarily because of the limited penetration of therapeutics across the blood-brain barrier (BBB) and the presence of immunosuppressive tumor microenvironments. To address these challenges, a HD-PEG@BM biomimetic nanoplatform (hereinafter referred to as HD-P@BM) is developed that cloaks the near-infrared II photosensitizer HD-PEG (HD-P) inside microglial membranes to enable enhanced BBB penetration and tumor-targeted delivery. In this study, it is found that the microglia-derived membranes enhanced the uptake of nanoparticles by both the glioma cells and tumor-associated microglia.

Diaphragm ultrasound for predicting weaning success in post-cardiac surgery acute respiratory distress syndrome patients: a prospective observational study in China.

Background: To explore the value of the diaphragm thickness fraction (TF) and diaphragm mobility (DM) measured by ultrasound for predicting ventilator withdrawal success in patients with acute respiratory distress syndrome (ARDS) after cardiac surgery.

Methods: This study included 246 patients undergoing the spontaneous breathing trial. Diaphragmatic function was evaluated by ultrasound, including the diaphragm thickness at the end of calm breathing (thickness of the diaphragm at functional residual capacity [TdiFRC]) and the maximum diaphragm thickness at the end of inspiration (thickness of the diaphragm at full vital capacity [TdiFVC]); TF=(TdiFVC-TdiFRC)/TdiFRC×100%.

Is integrative therapy of traditional Chinese medicine and progesterone capsule more effective than monotherapies in oligomenorrhea and hypomenorrhea? Evidence based on a multi-center randomized controlled trial and metabolomic profile.

Background And Objectives: Oligomenorrhea and hypomenorrhea were common conditions in outpatient visit which affected child-bearing aged women, and the conventional progesterone treatment has been related to several side effect and contradiction, and this study aimed to evaluate the effectiveness and safety of traditional Chinese medicine (TCM), progesterone capsules, and their combination in treating oligomenorrhea and hypomenorrhea both clinical and metabolomic approach.

Methods: A prospective, randomized, multi-center trial has been conducted, and a total of 239 oligomenorrhea and hypomenorrhea were randomly assigned to receive TCM, progesterone capsules (PG), or the combined Chinese and Western medicine (COM) and treated from 3 months, respectively. Scores were recorded at 1 and 3 month of treatment, while the seral samples for sex hormone and safety parameters were taken before and after management, with adverse events recorded.

Targeted next-generation sequencing (tNGS): An upcoming application for pathogen identification in clinical diagnosis.

Rapid and efficient detection of pathogens is a goal for clinical laboratories. Traditional methods such as post-culture identification, smear microscopy, antigen-antibody detection, and polymerase chain reaction cannot perform hundreds of high-throughput tests simultaneously. With the development of genetic engineering, next-generation sequencing technology has gradually been introduced into clinical testing.

Acid and weakly acidic reflux characteristics in pediatric airway malacia by pH and multiple intraluminal impedance monitoring: a retrospective cohort study.

Unlabelled: The objective of this study was to analyze the 24-h pH and multiple intraluminal impedance (pH-MII) monitoring results in pediatric patients with airway malacia, with the aim of comprehensively understanding the characteristics of gastroesophageal reflux (GER) in these patients and exploring their potential roles in the pathogenesis of airway malacia. This retrospective cohort study enrolled pediatric patients who underwent 24-h pH-MII monitoring and bronchoscopy at Maternal and Child Health Hospital of Hubei Province from January 2017 to October 2023. Among 64 pediatric patients with airway malacia, 24 (37.

Plasma ceramide mediates the association of peripheral T cells with Alzheimer's disease.

Introduction: Neuroinflammation involving peripheral immune cells, particularly T lymphocytes, has been implicated in Alzheimer's disease (AD) progression. Metabolic dysregulation in sphingolipid pathways may influence neuroinflammatory processes in AD pathogenesis. However, the specific roles of T cell subsets and their interactions with metabolic pathways in AD pathogenesis remain unclear.

Rural-Urban differentials in the factors associated with modern contraceptive usage among married reproductive-aged women in Hubei province, China.

Background: China's fast development is markedly impacting on migration, which has brought tremendous pressure and severe challenges to family planning services. Moreover, family planning (FP) is mostly regarded as women's responsibility by some Chinese cultures and societies. Several female contraceptives are easily accessible but only a few are known and widely used.

The implication of abnormal routine coagulation parameters in Dabie bandavirus infection.

Prolonged activated partial thromboplastin time (APTT) and thrombin time (TT), normal prothrombin time (PT) and elevated D-dimer level are typical manifestation of routine coagulation parameters in patients infected with Dabie bandavirus (DBV), infection-induced coagulation activation and consumption, and a heparin-like effect due to endogenous heparan are the common explanations. To understand the exact implication of abnormal coagulation parameters in patients infected with DBV and their correlation with bleeding tendency and prognosis. One hundred and twenty-one consecutive DBV-infected patients were enrolled in this prospective, single-center, observational study.

Decoding the impact of gut microbiota on heart failure.

Decreased cardiac output in heart failure leads to intestinal ischemia and increased permeability. Substantial changes occur in the gut microbiota, characterized by a decline in beneficial bacteria and an overgrowth of potentially harmful bacteria. The gut microbiota is intricately linked to prevalent risk factors for heart failure, including hypertension, diabetes, obesity, and renal insufficiency.

Integrated oral-gut microbiota therapy: a novel perspective on preventing bacterial translocation for systemic disease management.

Oral dysbiosis increases the risk of oral diseases and systemic diseases, with many related conditions overlapping with systemic diseases triggered by gut dysbiosis. Studies have shown that the oral cavity serves as an endogenous reservoir for gut microbial strains, influencing the homeostasis of both oral and gut microbiota through interactions involving bacterial translocation, microbial metabolites, immune cells, and inflammatory factors. In specific disease contexts, certain microbial communities [e.

STOML2 knockdown inhibits inflammation and airway remodeling of PDGF-BB-induced airway smooth-muscle cells by the MAPK pathway.

Objective: Asthma is a chronic inflammatory airway disease characterized by airway inflammation and remodeling. Abnormal proliferation and migration of human airway smooth muscle cells (HASMCs), induced by platelet-derived growth factor BB (PDGF-BB), are associated with the occurrence and progression of asthma. In this study, we aim to investigate the expression and molecular mechanisms of stomatin-like protein-2 (STOML2) in airway remodeling in asthma.

Random forest classification of four unsaturated fatty acids for infection diagnosis and prognosis in female Chinese patients.

Objective: Unsaturated fatty acids (UFAs) are important in immune regulation and inflammation; however, their diagnostic and prognostic value in infected patients is unclear. This study evaluated UFAs in infected female patients versus healthy controls using random forest (RF) analysis.

Methods: A total of 115 female subjects, consisting of healthy controls and infected patients, were recruited.

[Different diagnostic methods for necrospermia and correlation between the severity of sperm necrosis and sperm DNA fragmentation index].

Objective: To assess the value eosin-nigrosine (E-N) staining and hypo-osmotic swelling test (HOST) in the diagnosis of necrospermia and investigate the correlation of the degree of sperm necrosis with sperm DNA fragmentation index (DFI) and high DNA stainability (HDS).

Methods: Using computer-assisted　sperm analysis (CASA), we examined the sperm motility of 7 333 males seeking medical care in Maternal and Child Health Hospital of Hunan Province from May 2023 to July 2024, detected the sperm viability of those with asthenozoospermia by E-N staining and HOST, and measured the sperm DFI and HDS of those with necrospermia by sperm chromatin structure assay (SCSA). Based on sperm progressive motility (PR%), we divided the asthenozoospermia patients into three groups (PR% 20%－<30%, PR% 10%－<20% and PR% <10%), assessed the value of E-N staining and HOST in the diagnosis of necrospermia, and analyzed the correlation of the severity of sperm necrosis with sperm DFI and HDS.

Correlation of serum miR-203a-3p with the severity of neonates with necrotizing enterocolitis and analysis of its molecular mechanism.

Objectives: The objective of this study was to examine the role of microRNA-203a-3p (miR-203a-3p) in the pathogenesis of necrotizing enterocolitis (NEC).

Methods: Quantitative real-time polymerase chain reaction (qRT-PCR) and receiver operating characteristic (ROC) curve analysis were employed to evaluate the relative abundances of miR-203a-3p as well as its diagnostic capacity. Logistic regression was applied to search for influential risk factors associated with NEC in neonates.

Optical and electrochemical probes-assisted dual-mode nanosensor for ultrasensitive monitoring of serotonin in single cells.

5-Hydroxytryptamine (5-HT), a vital neurotransmitter, plays a critical role in the development of various neurodegenerative diseases. Thus, in situ tracking and monitoring of 5-HT levels in single living cells and their fluctuations under nonylphenol (NP)-induced stimulation are of great significance. Herein, we constructed a novel aptamer and methylene blue (MB)-assisted nanosensor (AM-NS) for in situ monitoring of 5-HT by combining electrochemical with fluorescence sensing.

Correction: CIN coexisting with AIS is a risk factor for residual disease after conization for cervical adenocarcinoma .

[This corrects the article DOI: 10.3389/fonc.2025.

A retrospective cohort study of gastrografin in the management of adhesive small bowel obstruction during pregnancy.

Adhesive small bowel obstruction (ASBO) during pregnancy is a critical condition that remains understudied. This study aimed to address management challenges of ASBO in pregnancy by evaluating the dual diagnostic and therapeutic role of gastrografin. We retrospectively analyzed medical records of pregnant patients with ASBO admitted between September 2018 and September 2023.

A deep learning-based computer-aided diagnosis system for detecting atypical endometrial hyperplasia and endometrial cancer through hysteroscopy.

Timely diagnosis of endometrial cancer (EC) and atypical endometrial hyperplasia (AEH) is crucial, yet traditional hysteroscopy faces accuracy challenges. This study introduces ECCADx, a deep learning-based computer-aided diagnosis system utilizing contrastive learning for hysteroscopic identification of AEH and EC. This is the system to integrate contrastive learning for this specific differentiation.