Association between CDSS score and 30-day mortality in nonAPL acute leukemia patients with intracranial hemorrhage: a cohort study.

Objectives: The aim of this study was to investigate the correlation between CDSS (Chinese DIC Scoring System) score and 30-day mortality in patients with intracranial hemorrhage (ICH) with nonacute promyelocytic leukemia (APL) acute leukemia.

Methods: This cohort study enrolled patients with non-APL acute leukemia complicated by ICH. The CDSS score was assessed in this patient population.

Severe pulmonary arterial hypertension development despite lupus nephritis remission: association with podocytic infolding glomerulopathy.

Background: Pulmonary arterial hypertension (PAH) associated with systemic lupus erythematosus (SLE) represents a significant complication with incompletely understood pathogenesis. Notably, podocytic infolding glomerulopathy (PIG), a rare pathological phenomenon in SLE-related renal lesions, has not been previously linked to PAH development. The cardiovascular safety profile of belimumab, a biologic agent for SLE treatment, requires continued surveillance, particularly regarding potential pulmonary vascular complications.

Synthesis and biological evaluation of novel nitrogen-containing heterocyclic derivatives as potential anticancer agents.

Based on screening results, a series of nitrogen-containing five-membered heterocyclic compounds were designed and synthesized for anticancer evaluation. Among them, 7 s, featuring a 1,2,3-triazole scaffold, exhibited the most potent antiproliferative activity against four human cancer cell lines (IC₅₀ < 10 μM). Mechanistic studies revealed that 7 s downregulated phosphorylated AKT (p-AKT) protein at 5 μM.

Ultrasound-enhanced Pt-coordinated polymer immunopotentiators and heterogenic fusion membrane-based multifunctional tumor vaccine nanoplatforms for melanoma treatment.

A tumor cell membrane (CM)-based biomimetic membrane tumor vaccine is an emerging prevention and treatment strategy in tumor immunotherapy. However, a single CM mostly has a weak immune-boosting effect. Here, a heterogenic fusion membrane tumor vaccine, EV-CM, was successfully constructed by fusing extracellular vesicles (EVs) from S.

Colloidal Nanoparticle Metasurfaces: Principles, Fabrication, and Applications.

Metasurfaces, a class of two-dimensional metamaterials, exhibit notable optical properties, making them attractive candidates for applications in fields such as the cloaking, biophotonics, and imaging. The realization of these applications is dependent on advancements in fabrication techniques. Conventional "top-down" lithography often faces limitations, such as polycrystallinity and surface roughness.

Single-Stranded Nucleic Acid-Induced Aggregation of Intrinsically Chiral Helical Gold Nanorods for Biosensing Applications.

Intrinsically chiral plasmonic nanoparticles exhibit unique three-dimensional chiral geometries with large specific surface areas and active sites. Although these structural features endow the nanoparticles with strong surface activity and optical chirality, they simultaneously lead to compromising colloidal stability. Consequently, this inherent instability enables significant sensing potential via controlled aggregation behavior.

Identification of novel pathogenic mutations in associated with distal renal tubular acidosis and analysis of wild-type expression in glomerular disease.

Distal renal tubular acidosis (dRTA) is a rare renal disorder caused by genetic mutations or secondary factors. If untreated, it can result in a significant electrolyte imbalance and progressive chronic kidney disease (CKD). This study investigated the key genes involved in renal tubular acid-base regulation (, and ) through whole exome sequencing (WES) in a clinical cohort.

"A perfect pair": extracellular vesicle-based novel strategies for precise cancer diagnosis and effective treatment.

Cancer remains a critical global health challenge, demanding innovative biomarkers and therapeutic targets for improved diagnosis and treatment. Extracellular vesicles (EVs), which are cell-secreted entities facilitating intercellular communication, have emerged as promising non-invasive biomarkers in oncology. This review focuses on the role of EVs in cancer diagnosis and therapy, covering their biogenesis, isolation, characterization, and functional implications.

Hypoxic Exosomal miRNAs From Skeletal Muscle Enhances Angiogenesis in Lower Limb Ischemia.

Lower limb ischemia in diabetic patients results in poor collateral circulation and a high risk of amputation. Skeletal muscle cells release exosomes that are believed to regulate angiogenesis; however, the underlying mechanisms remain unclear. This study examined the effects of exosomes derived from skeletal muscle cells on angiogenesis.

Lung cancer diagnosis through extracellular vesicle analysis using label-free surface-enhanced Raman spectroscopy coupled with machine learning.

Label-free surface-enhanced Raman spectroscopy (SERS) based on extracellular vesicles (EVs) has great potential in cancer diagnosis. However, the repeatability and stability of the SERS signals and the accurate early prediction of multiple cell types based on a small number of samples still require further research. We developed a highly accurate classification approach to distinguish EVs derived from lung cancer and normal cells.

Consistency of Ultrasound Measurements of Fat Thickness in Different Postures.

Introduction: Ultrasound has been used in the field of clinical nutrition to measure body composition. However, the consistency of these measurements varies across studies, and the impact of examination posture remains largely unexplored, creating a critical methodological gap in clinical practice. The purpose of this study was to investigate the consistency of ultrasonic measurement of fat thickness (FT) and evaluate the impact of posture on these measurements.

A cross-lagged network analysis of multisystemic factors influencing adolescent depressive symptoms: considering gender differences.

Background: Adolescent depression has become a pressing public health concern in China, with recent estimates indicating a prevalence rate of 26.17%. This mental health issue poses significant risks to adolescents' psychological development and long-term well-being.

A retrospective cohort study of gastrografin in the management of adhesive small bowel obstruction during pregnancy.

Adhesive small bowel obstruction (ASBO) during pregnancy is a critical condition that remains understudied. This study aimed to address management challenges of ASBO in pregnancy by evaluating the dual diagnostic and therapeutic role of gastrografin. We retrospectively analyzed medical records of pregnant patients with ASBO admitted between September 2018 and September 2023.

Gut microbiota shapes cancer immunotherapy responses.

The gut microbiota significantly influences cancer immunotherapy efficacy by modulating immune responses, remodeling the tumor microenvironment (TME), and producing key metabolites. Strategies such as FMT, probiotics, and dietary interventions show promise in enhancing responses to ICIs and ACTs while reducing immune-related adverse events (irAEs). This review summarizes clinical and preclinical findings and discusses microbiota-based interventions and future directions for precision immunotherapy.

sRNA-enriched outer membrane vesicles of Vibrio alginolyticus: Decisive architects of biofilm assembly.

Biofilms robustly defend bacteria by impeding antimicrobial entry, and protecting against environmental stress and immune responses, thereby enhancing host colonization. Overcoming the diagnostic and therapeutic challenges of biofilm infections is critical, given their clinical significance and resistance to standard treatments. Recent investigations have illuminated the pivotal role of outer membrane vesicles (OMVs) in biofilm formation.

CXCL12/CXCR4 modulates macrophage efferocytosis to induce glomerular crescent formation and fibrosis via ELMO1/DOCK180/RAC1 signaling in ANCA-associated glomerulonephritis.

Background: ANCA-associated glomerulonephritis (AAGN) is a leading cause of uremia in children, driven by macrophages (Mφs) that mediate crescent formation and fibrosis. Despite their critical role, the signals governing monocyte recruitment and macrophage polarization in AAGN remain unclear.

Methods: We utilized single-cell sequencing to dissect the cellular dynamics of AAGN and conducted in vitro and in vivo experiments to explore the role of the CXCL12/CXCR4 signaling axis in monocyte recruitment and macrophage efferocytosis.

Impact of diabetes mellitus on myocardial function and clinical outcomes in patients with significant aortic regurgitation.

Background: Type 2 diabetes mellitus (T2DM) causes myocardial dysfunction and has been linked to an increased risk of unfavorable cardiovascular events. However, the additive effects of T2DM on myocardial function and its association with clinical outcomes in patients with aortic regurgitation (AR) is undetermined. The study aimed to verify whether T2DM aggravates the deterioration of myocardial deformation and clinical outcomes in AR patients.

Theoretical models to guide undergraduate medical curriculum development: an integrative review.

Background: Higher medical education has experienced significant growth in recent years. Curriculum development has consistently been the focus of educators’ investigation, as it is one of the most critical components of educational reform. High-quality curriculum development must be founded on educators’ knowledge and experience and supported by scientific and standardized theoretical models.

Randomized, Positive-Controlled Study on the Efficacy and Safety of Oral Polysaccharide-Iron Complex Therapy in Patients on Hemodialysis.

Introduction: The IHOPE study evaluated the efficacy and safety of oral iron polysaccharide complex versus i.v. iron in Chinese patients on hemodialysis (HD).

Sono-activable and biocatalytic 3D-printed scaffolds for intelligently sequential therapies in osteosarcoma eradication and defect regeneration.

To mitigate the necessity for multiple invasive procedures in treating malignant osteosarcoma, an innovative therapeutic approach is imperative to achieve controllable tumor-killing effects and subsequent bone repair. Here, we propose the de novo design of sono-activable and biocatalytic nanoparticles-modified 3D-printed hydroxyapatite (HA) scaffold (HS-ICTO) for intelligently sequential therapies in osteosarcoma eradication and bone defect regeneration. The engineered HS-ICTO scaffold displays superior, spatiotemporally controllable HO-catalytic performances, which promptly generate massive reactive oxygen species via multienzyme-like mechanisms coupled with sono-activation, thus augmenting tumor cell apoptosis.

Quantification of normal skin thickness using very high-frequency ultrasound: a clinical study in Chinese adults.

Background: Measurement of skin thickness provides an objective basis for diagnosing skin diseases and evaluating treatment efficacy. High-frequency ultrasound (HFUS) offers the advantages of high frequency and resolution, enabling clear visualization of the epidermis, dermis. Very high-frequency ultrasound (VHFUS) boasts an even higher frequency and resolution, allowing for more precise imaging of each skin layer.

The biological roles and molecular mechanisms of m6A reader IGF2BP1 in the hallmarks of cancer.

N6-methyladenosine (m6A) is the most abundant and well-investigated internal RNA modification in eukaryotic RNAs, affecting its target gene expression by controlling RNA localization, splicing, stability, and translation. m6A modifications are regulated by m6A methyltransferase complex, demethylase, and reading proteins. Insulin-like growth factor-2 mRNA-binding protein 1 (IGF2BP1), a member of a conserved family of single-stranded RNA-binding proteins, has recently been identified as a vital m6A reading protein.

Multicenter phase 1/2 study of onatasertib, a dual TORC1/2 inhibitor, combined with the PD-1 antibody toripalimab in advanced solid tumors.

Preclinical studies have indicated that the combination of mTORC1/2 inhibitors with PD-1 antibodies exhibits synergistic effects on solid tumors. However, no clinical data supporting this combination have been reported. Therefore, we conducted a clinical trial (NCT04337463) to investigate the efficacy and safety of combining onatasertib, an mTORC1/2 inhibitor, with toripalimab, a PD-1 antibody in patients with advanced solid tumors.

Children's and caregivers' participation in the development of paediatric core outcome sets: a cross-sectional analysis.

Objectives: This study aims to address the status of children's and caregivers' participation in the development of paediatric core outcome sets (COS).

Methods: We included all paediatric COS from a previous systematic review and searched the Core Outcome Measures in Effectiveness Trials database to 26 February 2024 for recent paediatric COS. We used descriptive and thematic analysis methods to present the characteristics of the included COS and to describe children's and caregivers' participation in the development, including any facilitators and barriers.