Ultrasound-assisted minimally invasive repair of acute Achilles tendon rupture with a shuttling needle.

Background: The optimal management of acute Achilles tendon ruptures remains controversial. Open surgical repair (OSR) carries high complication rates, while minimally invasive techniques reduce risks but pose concerns regarding sural nerve injury. This study evaluates the clinical outcomes of ultrasound-assisted minimally invasive repair (MIR) using a double-ended shuttling needle.

Identification and Characterization of the Gene Family in Potato.

Carboxypeptidase A inhibitor (CPAI) is a globular polypeptide that specifically inhibits carboxypeptidase A activity in the insect gut, playing a vital role in plant defense against external stimuli. To date, this gene family has not been systematically characterized in potatoes. In this study, we identified the gene family using the potato DM v6.

Fine mapping and prediction of a candidate gene for wrinkled rind in melon (Cucumis melo L.).

The Cmwr locus was fine mapped to chromosome 2 in wrinkled-rind melon, and MELO3C010304.2 (CmNaa35, encoding an N-α-acetyltransferase) was identified as the most likely candidate gene for wrinkled rind in melon. The wrinkled rind (wr) phenotypic trait in melon is rare, and the regulatory mechanisms and genes governing this feature remain unclear.

Correction: CIN coexisting with AIS is a risk factor for residual disease after conization for cervical adenocarcinoma .

[This corrects the article DOI: 10.3389/fonc.2025.

A Weakly-Solvating Propylene Carbonate Electrolyte for High-Voltage and Low-Temperature Lithium-Ion Batteries.

Next-generation lithium-ion batteries (LIBs) require electrolytes compatible with high-voltage (>4.3 V) and low-temperature (<-10 °C) operation, yet commercial ethylene carbonate (EC)-based systems remain constrained by intrinsic limitations including poor oxidation stability at cathode side and high melting points. Although propylene carbonate (PC) demonstrates superior oxidative stability and lower melting temperature than EC, its tendency to cointercalate with Li within the graphite anode interlayers restricts its applicability in LIBs.

Ammonium-Generating Microbial Consortia in Paddy Soil Revealed by DNA-Stable Isotope Probing and Metatranscriptomics.

Rice paddy fields are sustainable agricultural systems as soil microorganisms help maintain nitrogen fertility through generating ammonium. In these soils, dissimilatory nitrate reduction to ammonium (DNRA), nitrogen fixation, and denitrification are closely linked. DNRA and denitrification share the same initial steps and nitrogen gas, the end product of denitrification, can serve as a substrate for nitrogen fixation.

Reusable SERS substrates based on flexible polyamide supports with hierarchical structures for in-situ detection of pesticide residues.

Performance optimization and functionality expansion of surface-enhanced Raman scattering (SERS) substrates are the main challenges towards their practical applications. Targeting the urgent need for rapid detection of trace pesticide residues in food safety monitoring, a reusable and highly sensitive SERS substrate based on soft polyamide (PA) fabric was developed. Dense ripple-like nanostructures, proven to significantly enhance SERS performance, were successfully created on the interwoven PA fibers through oxygen plasma (OP) treatment.

Curvulomycin E, a New Resorcylic Acid Lactone From the Beibu Gulf Coral-Derived Fungus Curvularia lunata GXIMD 02512.

One new resorcylic acid lactone (RAL), curvulomycin E (1), along with 10 known compounds (2-11), was obtained from the Beibu Gulf coral-derived fungus Curvularia lunata GXIMD 02512. Their structures were determined by extensive spectroscopic data interpretation and comparison with literature. The absolute configurations of 1 and 10 were accomplished by ECD calculations.

Effectiveness and safety of sirolimus in the treatment of venous malformations: A meta-analysis of prospective studies.

Background: Venous malformations are prevalent vascular anomalies. Recent clinical studies have explored the use of sirolimus for these conditions, particularly in patients who are not candidates for, or have shown limited response to, traditional treatments such as sclerotherapy and surgery. This meta-analysis systematically evaluates the efficacy and safety of sirolimus in treating venous malformations.

CIN coexisting with AIS is a risk factor for residual disease after conization for cervical adenocarcinoma .

Introduction: Compared to definitive hysterectomy, cervical conization with negative margin remains a controversial management for AIS currently. Our study aimed to evaluate (1) the effect of conization with negative margin alone or subsequent hysterectomy, (2) the effect of LEEP and CKC with or without subsequent hysterectomy, and (3) the correlation between clinicopathologic characteristics and residual disease after conization.

Methods: This retrospective study involved 59 AIS patients who had negative margins through conization, with or without subsequent hysterectomy, focusing on clinicopathologic characteristics and outcomes.

The CmCt gene induced reversibility of copper toxicity in melon.

Copper is crucial for plant growth and development, but excess copper can damage the leaves of melons.We constructed a genetic population using M625 (copper sensitive) and M972 (copper tolerant) materials for precise localization. Under copper stress, we quantified the levels of reactive oxygen species (ROS), antioxidant enzyme activities, hormone contents, copper accumulation, and conducted a detailed examination of leaf structural characteristics.

Key Role of Dissolved and Particulate Organic Matter on the Partitioning and Migration of Perfluoroalkyl Acids (PFAAs): A Case Study in the Heavily Polluted Laizhou Bay, China.

The partitioning behavior of perfluoroalkyl acids (PFAAs) between water and suspended particulate matter (SPM) can significantly influence their migration and fate in aquatic environments, yet the role of natural organic matter, including dissolved organic matter (DOM) and particulate organic matter (POM), in this process has rarely been explored. This study investigated the potential influences of DOM and POM on multiphase distribution process and migration of PFAAs. Results indicated that the origin and humification degree of DOM and POM were different, leading to distinct effects on the partitioning and migration of PFAAs.

Development of one-step multiplex real-time PCR for the detection of CHV-1, CAdV-2, and CDV.

Canine Infectious Respiratory Disease Complex (CIRDC) is a highly contagious disease that frequently affects canine populations and has emerged as a global epidemic. It has been reported that CIRDC can have a serious impact on related life. Therefore, the rapid detection and differentiation of common viruses that cause CIRDC are essential.

Betulinic Acid Delays Turnip Mosaic Virus Infection by Activating the Phytosulfokine Signalling Pathway in Nicotiana benthamiana.

Plant viral diseases pose a significant threat to agricultural production, and the availability of effective drugs against viral diseases remains limited. In this study, we discovered that betulinic acid (BA), a pentacyclic triterpenoid derived from plants, delays infection by turnip mosaic virus (TuMV) in Nicotiana benthamiana. Transcriptomic analysis revealed that BA treatment specifically induced the expression of N.

Structural Characterization and Anti-Inflammatory Effects of Oligosaccharides in Linn.

Guava ( Linn.) is a tropical fruit rich in carbohydrates, and its polysaccharides have been deeply studied. However, the extraction of guava oligosaccharides and the molecular mechanisms of anti-inflammatory activity are still inadequately explored.

A fijivirus capsid protein hijacks autophagy degrading an ω-3 fatty acid desaturase to suppress jasmonate-mediated antiviral defence.

Plant viruses often suppress jasmonic acid (JA)-mediated defences through disturbing JA signalling or biosynthesis pathways to benefit their own infection. Few studies have examined how the precursors of JA biosynthesis are regulated by viral infection. In this study, we demonstrate that rice black-streaked dwarf virus (RBSDV) infection inhibits the production of α-linolenic acid (C18:3), a key JA biosynthesis precursor that is catalysed by a set of fatty acid desaturases (FADs).

Molecular landscape of atherosclerotic plaque progression: insights from proteomics, single-cell transcriptomics and genomics.

Backgrounds: Atherosclerosis is a major contributor to cardiovascular diseases worldwide. Despite advancements in understanding its pathology, significant gaps remain in the molecular characterization of atherosclerotic plaques. This study addresses this gap by extensively profiling the proteomic landscape of carotid atherosclerotic plaques, classified under the American Heart Association (AHA) types IV to VI, to identify potential biomarkers and therapeutic targets.

Green and high-yield synthesis of bimetallic zeolitic imidazolate framework-67 for efficient catalytic CO cycloaddition.

Metal-organic frameworks (MOFs) such as ZIF-67 have been widely studied. Incorporating multi-bimetals into ZIF-67 is a strategic approach to leverage unique properties and address the limitations of traditional single metals in ZIF-67. Herein, an environmentally friendly solid-solid thermal (SST) method is developed to simultaneously incorporate heterometals (Cu, Fe, Ru, Pd, Mn, and Ni) into the ZIF structure, referred to as M@ZIF-67.

Single-cell spatial transcriptomics of tertiary lymphoid organ-like structures in human atherosclerotic plaques.

Tertiary lymphoid organs have been identified in the arterial adventitia in both mouse models of atherosclerosis and patients with atherosclerosis, yet their role in the disease remains insufficiently explored. Here we present a spatially resolved single-cell transcriptome atlas of human atherosclerotic plaques, identifying 14 distinct cell types and providing evidence of plaque tertiary lymphoid organs (PTLOs). The development of PTLOs was associated with the expression of lymphangiogenic chemokine genes and the adhesion molecule gene in fibroblast-like smooth muscle cells.

Climate change, geography and trade agreements: A perspective of Asian bilateral trade.

This study investigates the simultaneous effects of geographic factors, trade agreements, and climate change on bilateral exports in Asian countries. We estimate the correlation with bilateral exports by utilizing a panel data set from 2000 to 2020, employing various econometric techniques, particularly the structural gravity model. Therefore, this study aims to examine the simultaneous or complementary impact of influencing factors on exports and link them with the gross domestic product.

Optimizing treatment selection in venous malformations through imaging-assisted sequential therapy: A case series analysis.

Objective: Venous malformations (VMs) are the most prevalent slow-flow congenital vascular anomalies. Effective management of VMs often necessitates individualized approaches, particularly for lesions with significant interstitial components. This study aims to evaluate a sequential treatment strategy combining sclerotherapy and open surgery, with guidance from ultrasound (US) and magnetic resonance imaging (MRI).

Effect of Short-Term Exposure to Ambient Temperatures on Parkinson's Diseases Mortality Among Elderly Aged 60 Years and Above in China, 2013-2020.

Parkinson's disease (PD) is a prevalent neurodegenerative disorder with significant negative health and economic implications for individuals, families and society. This study utilized an individual-level time-stratified case-crossover study design to investigate the relationship between ambient temperatures and PD mortality among the elderly in China. A combination of conditional logistic regression and distributed lag non-linear model was employed to analyze the data, and the mortality burden attributed to ambient temperatures was quantified.

Deciphering the Effect of Postharvest 1-MCP Treatment Coupled with Low-Temperature Storage on the Physiological Activities and Edible Quality of Melon.

Fruits are an important source of a healthy diet due to their essential nutrients for daily intake. Melon is known as a significant fruit crop of the Cucurbitaceae family based on its various dietary benefits, but its shelf life needs to be maintained for long-term usage. 1-Methylcyclopropene (1-MCP) is a cyclopropene-derived synthetic plant growth regulator (PGR) that is used for significantly delaying the ripening process and maintaining the shelf life of climacteric fruits during storage.

Effects of the stress hormone norepinephrine on the probiotic properties of : antibacterial colonization, anti-inflammation, and antioxidation.

Probiotics as antibiotic alternatives are unstable for use under stress in clinical applications. To explore the influence of catecholamine hormones on probiotic bacterial inhibition and antimicrobial activity, we tested the effects of norepinephrine (NE) on and in a mouse model. The results showed that in the presence of NE, 80% of strains showed increased growth rate and more than 80% of the strains indicated lower antimicrobial activity at 22 h.

Identification and characterization of ClAPRR2, a key candidate gene controlling watermelon stripe color.

The stripe color of watermelon is a vital commercial trait and is the focus of attention of consumers and researchers. However, the genetic determinants of watermelon stripe color are incompletely understood. Based on the results of preliminary localization studies, we constructed a large-capacity F generation population (710 plants) using light-green striped ZXG1555 and green-striped Cream of Saskatchewan (COS) watermelon strains as parental lines for fine mapping.