Discontinuation of Glacial Meltwater Input Reshapes the Diversity and Stability of Eukaryotic Planktonic Microbial Communities in Glacial Lakes.

Glacial lakes play a vital role as indicators of global climate change and regional environmental responses. Eukaryotic planktonic microorganisms, pivotal in driving biogeochemical cycling of nutrients within these ecosystems, are crucial for preserving stability and ecological function of glacial lake environments. Nevertheless, the spatial and temporal dynamics, along with the mechanisms responsible for sustaining eukaryotic planktonic microbial communities in glacial lakes, especially during the glacier retreat and lake formation, are still largely uncharted.

In-depth structural characterization of sphingoid bases derivatization and electron-activated dissociation tandem mass spectrometry.

Sphingoid bases (SPHs) serve as the core structural backbone of all sphingolipid classes, with their diversity arising from intrachain modifications such as carbon-carbon double bonds (CC), hydroxyl groups, and methyl branching. Traditional tandem mass spectrometry (MS/MS) relying on collision-induced dissociation (CID) often fails to yield diagnostic fragmentation patterns for precise localization of these modifications, underscoring the need for advanced dissociation techniques. In this work, we present a novel analytical strategy combining carnitine derivatization of sphingoid amines with electron-activated dissociation (EAD) in MS to enable in-depth structural characterization.

Innovative therapies for diabetic foot ulcers: Application and prospects of smart dressings.

Diabetic foot ulcers (DFUs), as one of the most severe complications of diabetes mellitus, have emerged as a critical global public health challenge due to their high incidence, refractory nature, and serious adverse outcomes. Clinical evidence demonstrates that while conventional therapies-including wound debridement, pharmacological treatment, and surgical intervention-can partially improve wound healing, they remain limited by suboptimal efficacy and high complication rates. In recent years, breakthroughs in smart dressing technology, enabled by advanced materials and biosensing innovations, have provided transformative solutions to this clinical dilemma.

Correction: Huang et al. The Safety and Immunogenicity of a Quadrivalent Influenza Subunit Vaccine in Healthy Children Aged 6-35 Months: A Randomized, Blinded and Positive-Controlled Phase III Clinical Trial. 2025, 467.

The authors would like to make the following corrections to this published paper [...

Multi-omics dissection of metabolic and transcriptional regulation underlying fruit maturation in Panax ginseng.

Background: Panax ginseng is a perennial plant valued for its medicinal and nutritional properties. Its fruit contains a variety of bioactive compounds such as ginsenosides, flavonoids, phenolic acids, and anthocyanins. However, the regulatory mechanisms underlying the accumulation of these compounds during fruit development remain largely unexplored.

Clinical heterogeneity, treatment patterns, and outcomes of advanced-age-onset myasthenia gravis in China: a retrospective cohort study.

Objectives: The clinical characteristics and outcomes of myasthenia gravis (MG) with onset at age 50 years or older, which commonly referred to as late-onset MG (LOMG), in elderly patients remain incompletely understood. This study aimed to address the clinical heterogeneity within LOMG and to clarify whether very-late-onset MG (≥70 years) constitutes a distinct phenotype requiring tailored management approaches.

Methods: We analyzed data from 2783 patients enrolled in the MG Registry between April 1, 2009, and December 31, 2020.

LAMP-Based 4-Channel Microfluidic Chip for POCT Detection of Influenza A H1N1, H3N2, and Influenza B Victoria Viruses.

: Influenza viruses are major pathogens responsible for respiratory infections and pose significant risks to densely populated urban areas. RT-qPCR has made substantial contributions in controlling virus transmission during previous COVID-19 epidemics, but it faces challenges in terms of detection time for large sample sizes and susceptibility to nucleic acid contamination. : Our study designed loop-mediated isothermal amplification primers for three common influenza viruses: A/H3N2, A/H1N1, and B/Victoria, and utilized a 4-channel microfluidic chip to achieve simultaneous detection.

Ginsenoside Rb1 attenuates neuroflammation via activating Wnt/β-catenin signaling pathway to exert neuroprotective effect on cerebral ischemic-reperfusion injury.

Purpose: To explore the molecular mechanism of G-Rb1 regulating microglia polarization through Wnt/β-catenin signaling pathway to alleviate cerebral ischemia-reperfusion injury in mice.

Methods: C57BL/6J mouse middle cerebral artery occlusion/reperfusion (MCAO/R) model and microglia (BV2) oxygen-glucose deprivation/reoxygenation (ODG/R) model were used. The neuroprotective effect of G-Rb1 and was evaluated by measuring nerve function deficit, cerebral blood perfusion recovery, infarct volume and cell viability.

Sex Hormone Androgen Elevates Blood Pressure Through Gut Microbiota-TMAO Pathway.

Background: Hypertension is a leading risk factor for all-cause mortality worldwide, affecting ≈1.3 billion people. Imbalanced gut microbiota contributes to blood pressure elevation.

Discovery of novel calcium-chelating peptides from Antarctic krill: Structural elucidation and dynamic coordination mechanisms.

Calcium-chelating peptides (CCPs) represent promising strategies for addressing calcium deficiency, yet their chelating mechanisms require further elucidation. This study identified two novel CCPs, EEDLER and IVELEEE, from Antarctic krill through enzymatic hydrolysis combined with multi-step separation and purification, exhibiting calcium-chelating capacities of 12.1 ± 1.

Multi-scale rheology for emulsion stability investigation: Bulk-, interfacial-, and micro-perspectives.

Emulsions are hierarchically structured complex fluids, whose physical stability plays an imperative role in their production and application. The rheology at different length-scales (macroscopic: bulk rheology; mesoscopic: interfacial rheology; microscopic: microrheology) are powerful tools to comprehensively investigate the emulsion stability from a multi-scale point of view. A lack of systematic knowledge on the suitability of all the three rheological techniques, which have been progressing rapidly in recent years, for emulsion stability characterization underscores the necessity of this review.

Gold-Catalyzed Highly Regioselective Cage B(9)-H Alkylation of -Carboranes with 1,6-Enynes.

Described herein is a gold-catalyzed regioselective B(9)-H alkylation of -carboranes via regioselective insertion of -cyclopropyl gold carbenes, generated in situ by gold-catalyzed cyclization of 1,6-enynes, into the cage B(9)-H bond of -carboranes. This new protocol furnishes a broad range of B(9)-bicyclo[3.1.

Genome-wide association study and molecular marker development for resistance to soybean cyst nematode in soybean.

Soybean (Glycine max) is a major crop for grain and oil production worldwide. However, soybean cyst nematode (SCN) disease is a serious threat to soybean cultivation, causing major challenges for agriculture in China and globally. In this study, 306 soybean germplasms were evaluated for resistance to SCN HG type 0 using the female index (FI), a metric quantifying nematode reproduction on soybean roots.

Peptidomics and molecular docking reveal digestion-resistant IgE-binding epitopes in bovine β-lactoglobulin and α-lactalbumin from simulated infant digestion.

β-Lactoglobulin (BLG) and α-lactalbumin (ALA) are the primary allergens in whey protein. However, gastrointestinal digestion can induce structural rearrangements of their IgE-binding epitopes, complicating the precise evaluation of allergenicity. This study developed a novel approach to identify digestion-resistant antigenic epitopes of BLG and ALA through simulated in vitro infant digestion.

Metabolic landscape uncovers remodeling of T cell immunity affected by fatty acid desaturase in Parkinson's disease at single-cell resolution.

Background: Peripheral activated T cells cross the blood-brain barrier, partake in neuroinflammation, and induce dopaminergic neuron degeneration through characteristics such as cell adhesion and immune response in Parkinson's disease (PD). Metabolic activity, which can regulate and be regulated by cellular signaling pathways, has a profound impact on the differentiation and function of T cells. However, a characterization of T-cell metabolic heterogeneity at single-cell resolution in PD is still lacking.

Association between cardiometabolic index and self-reported symptoms of obstructive sleep apnea: a cross-sectional study.

The Cardiometabolic Index (CMI) is a new indicator of visceral fat distribution and dysfunction that can be calculated using lipid parameters, waist circumference, and height. The relationship between CMI and Obstructive Sleep Apnea (OSA) remains unclear. This cross-sectional study explored the association between CMI and OSA using nationally representative data from The National Health and Nutrition Examination Survey database, spanning the periods from 2005 to 2008 and 2015 to 2018.

CRISPR-based assays for the detection of BK virus and JC virus infections post-kidney transplantation.

Background: Organ transplantation recipients encounter significant risks from acute or chronic infections that threaten graft survival. BK virus (BKV) and JC virus (JCV) are two prominent opportunistic infection viruses, and they may cause polyomavirus-associated nephropathy and graft kidney loss in patients who are in an immunosuppressed state after kidney transplantation. Hence, timely detection and sustained monitoring of the viral load are indispensable.

Enhancing anthocyanin stability via co-assembled nanoencapsulation with tripeptides by intermolecular interaction.

The high sensitivity of anthocyanins to environmental stressors poses significant challenges for their application in food. Encouraging progress has been made with peptide encapsulation strategies, which have enhanced the stability of anthocyanin. In this study, we employ cost-effective tripeptides for nanoencapsulation of anthocyanins and investigate the fundamental interactions between the peptides and anthocyanins using a simplified model that minimizes the influence of multiple amino acids.

Multi-Step Natural Gas Load Forecasting Incorporating Data Complexity Analysis with Finite Features.

Data complexity directly affects the dynamics of complex systems, which in turn influences the accuracy and robustness of forecasting models. However, the load data exhibit complex features such as self-similarity, long-term memory, randomness, and chaos. This study aims to quantify and evaluate the complexity features of natural gas loads and to develop a multi-step-ahead forecasting model that integrates data decomposition and ensemble deep learning while considering these complexity features.

Anthocyanin profiles and transcriptional control in anthocyanin-rich Brassica carinata.

Brassica carinata has gained traction as an alternative biofuel feedstock in many countries, and serves as a well-known dual-purpose crop for both oilseed and leafy vegetable production. The purple varieties, which are rich in anthocyanins, are usually more eye-catching and beneficial to health. In this study, eleven cyanidin 3-glycoside-5-glucoside derivatives with different acyl modifications were characterized in two purple varieties of B.

Bioactive Peptides Derived from Tuna: Screening, Extraction, Bioactivity, and Mechanism of Action.

Peptides play a crucial role in the development of pharmaceuticals and functional foods. Multiple studies have shown that natural bioactive peptides possess antioxidant, antihypertensive, anti-tumor, and anti-inflammatory activities. Marine bioactive peptides, especially those sourced from fish, constitute a substantial reservoir of these molecules.

Potent β-lactam-based tyrosyl-DNA phosphodiesterase 1 inhibitors identified by a virtual screen.

Tyrosyl-DNA phosphodiesterase 1 (TDP1) is an important therapeutic target. We recently reported several crystal structures of TDP1 with small molecules bound within the catalytic pocket. These molecules bind by forming hydrogen bonds with residues of the catalytic HKN motifs.

Developmental riboflavin deficiency results in structural and functional changes in the neural retina and RPE.

The retina, a metabolically active tissue, relies on adequate flavin levels for optimal function. Our previous research demonstrated that ablation of RTBDN, a retina-specific riboflavin binding protein, plays a pivotal role in maintaining flavin levels, leading to progressive retinal degeneration. This raises the fundamental question of how riboflavin deficiency impacts retinal structure and function.

Efficient Microbial Production of Selenium Nanoparticles by Selenium-Tolerant Alcaligenes sp. HZ-9-3-3.

Selenium (Se) is a crucial trace element for human and animal health, contributing significantly in various physiological processes. However, its uneven global distribution leads to widespread deficiencies. Nano-selenium (selenium nanoparticles, SeNPs), with low toxicity and high bioavailability, emerges as a promising alternative, and microbial synthesis is the preferred method for its production.

Bronze and Iron Age genomes reveal the integration of diverse ancestries in the Tarim Basin.

The Tarim Basin in Xinjiang functioned as a crucial meeting point for peoples, cultures, and goods across the Eurasian steppe and served as the easternmost edge for western steppe population dispersal. Despite its importance as a historical crossroads, the prehistoric genetic history of the region remains largely unexamined, which results in a major gap in understanding Eurasian population movements and steppe group expansion to the east. Here, we present genome-wide data from 24 individuals from the western Tarim Basin during the Bronze and Iron Ages.