Brucella-infected macrophage-derived Exosomes enhance the anti-Brucella acquired immune response.

In recent years, the roles of Exosomes in tumors and infectious diseases have been found to have potential application value in disease diagnosis and treatment. However, whether Exosomes are involved in host-acquired immune responses against Brucella has not been reported. This study explored the significance of Exosomes in the context of Brucella infection and their influence on host immune responses.

Enzyme-independent functions of HDAC3 in the adult heart.

The cardioprotective effects of histone deacetylase (HDAC) inhibitors (HDIs) are at odds with the deleterious effects of HDAC depletion. Here, we use HDAC3 as a prototype HDAC to address this contradiction. We show that adult-onset cardiac-specific depletion of HDAC3 in mice causes cardiac hypertrophy and contractile dysfunction on a high-fat diet (HFD), excluding developmental disruption as a major reason for the contradiction.

Recognition of pivotal immune genes NR1H4 and IL4R as diagnostic biomarkers in distinguishing ovarian clear cell cancer from high-grade serous cancer.

Objective: Ovarian clear cell carcinoma (OCCC) is characterized by poor prognosis and limited early diagnostic markers. Identifying molecular distinctions between OCCC and the more common high-grade serous ovarian cancer (HGSC) is critical to developing targeted diagnostic and therapeutic strategies for improved clinical outcomes.

Methods: We retrieved the mRNA expression profiles of OCCC and HGSC from the Gene Expression Omnibus (GEO) database.

Research progress of ECT2 and RhoA-related signaling pathways in gynecological tumors.

Epithelial Cell Transformation Factor 2 (ECT2) is highly expressed in a variety of cancers, including gynecological tumors. The mislocalization of ECT2 can abnormally activate Ras homolog family member A (RhoA) in the Ras homolog gene family (Rho) Guanine nucleotide Exchange Factor (GEF) family. Activated RhoA binds to Rho-associated protein kinase (ROCK), phosphorylates various target proteins, triggers a cascade reaction, regulates the functions of downstream proteins, and thereby plays an important role in the occurrence and development of tumors.

Genetic Dissection of Drought Tolerance in Maize Through GWAS of Agronomic Traits, Stress Tolerance Indices, and Phenotypic Plasticity.

Drought severely limits crop yield every year, making it critical to clarify the genetic basis of drought tolerance for breeding of improved varieties. As drought tolerance is a complex quantitative trait, we analyzed three phenotypic groups: (1) agronomic traits under well-watered (WW) and water-deficit (WD) conditions, (2) stress tolerance indices of these traits, and (3) phenotypic plasticity, using a multi-parent doubled haploid (DH) population assessed in multi-environment trials. Genome-wide association studies (GWAS) identified 130, 171, and 71 quantitative trait loci (QTL) for the three groups of phenotypes, respectively.

[Clinical and genetic analysis of a patient with Loeys-Dietz syndrome caused by a SMAD3 gene variant].

Objective: To explore the genetic basis of a patient suspected for Loeys-Dietz syndrome (LDS).

Methods: A adult male patient with aneurysmal dilation of the aortic root identified during the treatment for chronic myeloid leukemia at Anzhen Hospital of Capital Medical University in 2021 was selected as the study subject. Clinical data of the patient were retrospectively collected.

A novel IgG-Fc-Fused multiepitope vaccine against Brucella: robust immunogenicity.

Brucellosis is one of the most common zoonotic diseases caused by Brucella spp. However, there is currently no Brucella vaccine available for humans. Although some attenuated live vaccines have been approved for animals, their protective efficacy is suboptimal.

Genome and Pathogenicity Analysis of an NADC30-like PRRSV Strain in China's Xinjiang Province.

The porcine reproductive and respiratory syndrome virus (PRRSV) possesses an inherent ability to adapt to environmental transformations and undergo evolutionary changes, which has imposed significant economic pressure on the global pig industry. Given the potential for recombination among PRRSV genomes and variations in pathogenicity, newly emerging PRRSV isolates are of considerable clinical importance. In this study, we successfully isolated a novel strain named XJ-Z5 from PRRSV-positive samples collected in Xinjiang province in 2022.

Scale-up synthesis of amorphous Ni-Fe tungstate for highly-efficient oxygen evolution reaction.

NiFe-based materials have been widely explored as electrocatalysts for the oxygen evolution reaction (OER). However, it remains a challenging task to simultaneously improve their catalytic performance, stability, and feasibility of scale-up production. Herein, a microimpinging stream reactor (MISR) is constructed for the scale-up synthesis of amorphous Ni-Fe tungstate (NiFeWO-3) powder as an active OER electrocatalyst.

Genome-Wide Identification and Expression Analysis of the Phosphate Transporter Gene Family in Under Phosphorus Stress.

Phosphorus is one of the key limiting factors for maize growth and productivity, and low-phosphorus stress severely restricts crop yield and stability. Enhancing the ability of maize to grow under low-phosphorus stress and improving phosphorus use efficiency (PUE) are crucial for achieving high and stable yields. Phosphate transporter (PHT) family proteins play a crucial role in the absorption, transport, and utilization of phosphorus in plants.

Evaluation of the safety and immune protection of OMPAC, PAPF, and EBPSs recombinant subunit vaccines Developed for Escherichia coli, Staphylococcus aureus, and Streptococcus agalactiae in mice.

Bacterial mastitis in dairy cow is often caused by a combination of bacterial infections, such as Escherichia coli, Staphylococcus aureus, and Streptococcus agalactiae. Currently, there is no effective vaccine against the disease. Therefore, we constructed a recombinant subunit vaccine by fusing gene fragments of E.

Coronary Artery Bypass Grafting Plus Mitral Valve Plasty May Not Provide More Advantage in Patients with Coronary Heart Disease and Moderate Ischemic Mitral Regurgitation: An Inverse Probability of Treatment Weighting Retrospective Cohort Study.

Objective: To compare the efficacy of isolated off-pump coronary artery bypass grafting (OPCABG) and of coronary artery bypass grafting (CABG) plus mitral valve plasty (MVP) in treating coronary heart disease with moderate ischemic mitral regurgitation to find a better surgical method.

Methods: Clinical data of 822 patients diagnosed with coronary heart disease and moderate ischemic mitral regurgitation were analyzed retrospectively. Patients were divided into the OPCABG and CABG+MVP groups according to surgical methods.

Prevalence of dysphagia following posterior fossa tumor resection: a systematic review and meta‑analysis.

Objective: Dysphagia is common in individuals who have undergone posterior fossa tumor (PFT) resection and negatively impacts on the individual's quality of life, nutritional status, and overall health. We aimed to quantitatively synthesize data from studies of the prevalence of dysphagia following PFT resection.

Methods: PubMed, Web of Science, the Cochrane Library, Embase, China National Knowledge Infrastructure (CNKI), Wanfang database, and VIP database were searched for case-control and cross-sectional studies that evaluated the prevalence of dysphagia after PFT surgery.

AuBr Induces CsPb(Br/I) QDs to Self-Assemble into Nanowires.

Perovskite quantum dots can form various forms such as nanowires, nanorods, and nanosheets through self-assembly. Nanoscale self-assembly can be used to fabricate materials with excellent device properties. This study introduces AuBr into CsPb(Br/I) quantum dots, causing them to assemble into nanowires.

In-situ gel injection of poloxamer-based metamizole provides long-acting antipyretic effects.

Metamizole easily decomposes in the body and has a short action time and low bioavailability. Hence, frequent injection administrations are needed to maintain its plasma concentration. This study aimed to design and develop an in-situ gel based on poloxamer 407 and 188 to assess its long-acting antipyretic effects.

Application of biofilm dispersion-based nanoparticles in cutting off reinfection.

Bacterial biofilms commonly cause chronic and persistent infections in humans. Bacterial biofilms consist of an inner layer of bacteria and an autocrine extracellular polymeric substance (EPS). Biofilm dispersants (abbreviated as dispersants) have proven effective in removing the bacterial physical protection barrier EPS.

A case report of sepsis associated coagulopathy after percutaneous nephrostomy.

Background: Hemorrhage is a common complication of nephrostomy and percutaneous nephrolithotripsy, and it is caused by surgical factors. Here we report a rare case of hemorrhage caused by sepsis-related coagulation dysfunction.

Case Presentation: A 72-years-old male patient with bilateral ureteral calculi accompanied by hydronephrosis and renal insufficiency developed sepsis and hemorrhage on the third day after bilateral nephrostomy.

A multi-epitope subunit vaccine based on CU/ZN-SOD, OMP31 and BP26 against Brucella melitensis infection in BALB/C mice.

Brucellosis, a zoonosis caused by Brucella, is highly detrimental to both humans and animals. Most existing vaccines are live attenuated vaccines with safety flaws for people and animals. Therefore, it is advantageous to design a multi-epitope subunit vaccine (MEV) to prevent Brucella infection.

Enhancement of dissolution rate and oral bioavailability of poorly soluble drug florfenicol by using solid dispersion and effervescent disintegration technology.

Objective: Florfenicol(FF) is an excellent veterinary antibiotic, limited by poor solubility and poor bioavailability.

Significance: Here in, we aimed to explore the applicability of fast disintegrating tablets compressed from Florfenicol-loaded solid dispersions (FF-SD-FDTs) to improve the dissolution rate and oral bioavailability of Florfenicol.

Methods: Utilizing selecting appropriate preparation methods and carriers, the solid dispersions of Florfenicol (FF-SDs) were prepared by solvent evaporation and the fast disintegrating tablets (FF-SD-FDTs) were prepared by the direct compression (DC) method.

Dynamic changes in cardiac morphology, function, and diffuse myocardial fibrosis duration of diabetes in type 1 and type 2 diabetic mice models using 7.0 T CMR and echocardiography.

Background: Diabetes mellitus (DM) is associated with an increased risk of cardiovascular disease (CVD). Hence, early detection of cardiac changes by imaging is crucial to reducing cardiovascular complications.

Purpose: Early detection of cardiac changes is crucial to reducing cardiovascular complications.

[Genetic testing and clinical analysis of a patient with Dilated cardiomyopathy due to variant of FLNC gene].

Objective: To explore the genetic basis for a patient with Dilated cardiomyopathy.

Methods: A patient admitted to Beijing Anzhen Hospital Affiliated to Capital Medical University in April 2022 was selected as the study subject. Clinical data and family history of the patient was collected.

[Clinical and genetic analysis of a patient with Loeys-Dietz syndrome due to variant of TGFBR2 gene].

Objective: To explore the genetic basis of a patient with clinically suspected Loeys-Dietz syndrome (LDS).

Methods: A child who had presented at Beijing Anzhen Hospital in September 2018 was selected as the study subject. Clinical data and family history of the patient were collected, along with peripheral blood samples of the proband and his parents.