CRISPR/Cas9 screening identifies SUV39H2 as a key regulator of oHSV-1 resistance in oral squamous cell carcinoma.

Oncolytic viruses represent an innovative strategy for cancer therapy. However, extensive gene expression reprogramming within tumor cells may hinder viral propagation by affecting essential cell-virus interactions. Here, through genome-wide CRISPR/Cas9 library screening, Suppressor of variegation 3-9 homolog 2 (SUV39H2), a histone methyltransferase, was identified as a critical factor in mediating resistance to oncolytic herpes simplex virus 1 (oHSV-1) in oral squamous cell carcinoma (OSCC).

Predictors and Prognosis of Mild Regurgitation After Rheumatic Mitral Valve Repair: A Dual-Center Cohort Analysis Based on Cardiac Computer Tomography.

Objectives: Rheumatic mitral disease (RMD) remains a significant health issue, especially in developing regions. Although mitral valve (MV) repair improves outcomes, residual mild mitral regurgitation (MR) is common. This study aims to identify clinical and anatomical factors associated with residual mild MR after MV repair and to assess its impact on MR progression and survival.

Fusion of SARS-CoV-2 neutralizing LCB1 peptide with Bacillus amyloliquefaciens RNase improves antiviral efficacy.

Virus-neutralizing peptides (VNPs) emerged as promising antiviral drug candidates with unprecedented specificity and cost-effectiveness during the recent COVID-19 pandemic. However, limited avidity, lack of effector functions, short circulatory half-life, and restricted administration routes make them inferior compared to neutralizing antibodies. To address these constraints, a potent VNP that targets the SARS-CoV-2 S protein is combined with Barnase, a highly active RNA-cleaving enzyme from Bacillus amyloliquefaciens.

The crystal structure of coronavirus RBD-TMPRSS2 complex provides basis for the discovery of therapeutic antibodies.

HCoV-HKU1, one of seven human coronaviruses (HCoVs) that have harmful effects on human health, accounts for a substantial portion of common cold cases and can cause severe respiratory diseases in certain populations. Currently, effective antiviral treatments against this virus are limited. Recently, TMPRSS2, a host protease long acknowledged for its role in priming the spike proteins of various CoVs and promoting viral entry, was identified as a functional receptor for HCoV-HKU1, opening an avenue for anti-HCoV-HKU1 therapy development.

Molecular characteristics of human adenovirus isolated from the 2024 influenza-like illness outbreaks in Suzhou City, China.

Background: Human adenoviruses (HAdV) comprises a genetically diverse group of double-stranded DNA viruses strongly associated with influenza-like illness (ILI) and acute respiratory illnesses (ARI) outbreaks. This study aimed to elucidate the molecular characteristics of HAdV implicated during ILI outbreaks in Suzhou City, Jiangsu Province in 2024.

Methods: Throat swab samples were gathered from settings with ILI outbreaks in Suzhou between March 2024 and January 2025.

Cardiac magnetic resonance comparison of non-dilated and dilated cardiomyopathy: imaging features and prognostic predictors in non-dilated left ventricular cardiomyopathy.

Objectives: Non-dilated left ventricular cardiomyopathy (NDLVC) is a novel cardiomyopathy characterised by normal LV size and non-ischaemic myocardial scarring or fatty tissue replacement. This study aimed to explore the clinical and cardiac magnetic resonance (CMR) characteristics of NDLVC compared with dilated cardiomyopathy (DCM) and event rates of patients with NDLVC and reduced LV ejection fraction (NDLVC-REF).

Materials And Methods: A retrospective cohort study of 396 patients, including 210 with NDLVC (135 classified as NDLVC-REF) and 186 with DCM, who underwent CMR imaging between 2015 and 2017, was conducted.

Preoperative multimodal imaging evaluation of a primary cardiac schwannoma of the right atrioventricular groove: a case report.

Background: Cardiac schwannoma is exceedingly rare, and few literature reports are available. We reported a case of primary cardiac schwannoma that performed preoperative multimodal imaging, aiming to highlight the significance of multimodal imaging evaluation and deepen our understanding of this tumour.

Case Summary: A 66-year-old man presented to our hospital, as physical examination revealed the presence of a mediastinal mass over a month.

Reducing the V Loss of Hole Transport Layer-Free Carbon-Based Perovskite Solar Cells via Dual Interfacial Passivation.

The hole transport layer (HTL)-free carbon-based perovskite solar cells (C-PSCs) are promising for commercialization owing to their excellent operational stability and simple fabrication process. However, the power conversion efficiencies (PCE) of C-PSCs are inferior to the metal electrode-based devices due to their open-circuit voltage (V) loss. Herein, time-resolved confocal photoluminescence microscopy reveals that grain boundary defects at the perovskite/carbon interface are very likely to function as nonradiative recombination centers in HTL-free C-PSCs.

Analysis of Risk Factors Associated with Left Ventricular Thrombus in Patients with Ischemic Ventricular Aneurysm: A 3.0 T Cardiac Magnetic Resonance Study.

Rationale And Objectives: Ischemic left ventricular aneurysm (LVA) is associated with left ventricular thrombus (LVT), which poses a significant risk of systemic embolism. This retrospective study aimed to identify clinical and cardiac magnetic resonance (CMR)-related risk factors for LVT in patients with ischemic LVA.

Materials And Methods: This study included consecutive hospitalized patients who underwent CMR for ischemic LVA between September 2015 and June 2024.

Study on the potential links between clinical features of oral lichenoid mucositis and systemic diseases.

Objective: This study aimed to analyze the clinical characteristics of oral lichenoid disease and investigate its potential association with systemic diseases.

Methods: This study was a retrospective cross-sectional analysis. The study comprised 116 patients who had been diagnosed with oral lichenoid disease, including 70 with oral lichen planus and 46 with lichenoid lesions.

Modular Design of T Cell Nanoengagers for Tumor Immunotherapy via Genetically Engineered Lipid-Tagged Antibody Fragments.

T cell engagers, which bind tumor-associated antigens and T cell specific molecules, represent a promising class of immunotherapies for enhancing targeted immune responses. Here, a "plug-and-display" platform is introduced for engineering T cell nanoengagers by anchoring antibody fragments into lipid-based nanoparticles. This approach utilizes a genetically engineered lipoprotein fused with single-chain variable fragments (scFv) and nanobodies, which spontaneously integrate into lipid bilayer of the nanoparticles, achieving a high surface density of at least 0.

Deep Learning and Radiomics Discrimination of Coronary Chronic Total Occlusion and Subtotal Occlusion using CTA.

Rationale And Objectives: Coronary chronic total occlusion (CTO) and subtotal occlusion (STO) pose diagnostic challenges, differing in treatment strategies. Artificial intelligence and radiomics are promising tools for accurate discrimination. This study aimed to develop deep learning (DL) and radiomics models using coronary computed tomography angiography (CCTA) to differentiate CTO from STO lesions and compare their performance with that of the conventional method.

Hepatobiliary phase manifestations of breast cancer liver metastasis: differentiating molecular types through Gd-EOB-DTPA-enhanced MRI.

Objective: The primary objective of this study is to evaluate the diagnostic efficacy of gadolinium ethoxybenzyl diethylenetriaminepentaacetic acid (Gd-EOB-DTPA) -enhanced magnetic resonance imaging (MRI) in distinguishing breast cancer liver metastasis (BCLM) across different molecular types.

Methods: Between August 2014 and July 2021, a cohort of 270 patients histologically diagnosed with BCLM underwent examination through dynamic contrast-enhanced MRI (DCE-MRI). The data collection encompassed clinical information of patients, as well as information on the quantity, shape, boundary, and fusion state of liver metastases.

ISG15 depletion enhances oHSV-1 replication and antitumor efficacy in oral squamous cell carcinoma.

Oncolytic viruses (OVs) represent a promising experimental therapy for a range of cancers, including oral squamous cell carcinoma (OSCC). In this study, oHSV refers to an oncolytic virus engineered from HSV-1(Herpes Simplex Virus Type 1). The oHSV-1 is an oncolytic virus derived from HSV-1, where both copies of the ICP34.

Association of pericoronary inflammation with atherosclerotic plaque progression in diabetic patients with improved modifiable cardiovascular risk factors: a longitudinal CCTA cohort study.

Background: Pericoronary adipose tissue (PCAT) attenuation, as assessed by coronary computed tomography angiography (CCTA), has been identified as a marker of pericoronary inflammation and a predictor of future adverse atherosclerotic events. However, the impact of changes in PCAT attenuation, as evaluated by consecutive CCTAs, on plaque progression in high-risk atherosclerotic patients with improved modifiable cardiovascular risk factors (mCRFs) remains unclear.

Methods: Consecutive patients with type 2 diabetes mellitus (T2DM) who had improved mCRFs and underwent serial, clinically indicated CCTA examinations (time interval ≥ 12 months) at our center between July 2019 and July 2022 were screened.

Clinical features of cystic neutrophil granulomatous mastitis in 62 cases.

Background: Cystic neutrophilic granulomatous mastitis (CNGM) is a rare breast inflammatory condition. Despite its rarity, understanding its pathogenesis and clinical features is crucial for accurate diagnosis and effective management. This study delves into the characteristic of CNGM.

Musculoskeletal ultrasound-guided needle knife therapy in the treatment of refractory nonspecific low back pain: A single-blind, randomized controlled trial.

Background: This clinical study aimed to evaluate the Numerical Rating Scale (NRS), Oswestry disability index (ODI), and Japanese Orthopedic Association (JOA) scores at 2, 4, 6, and 12 weeks, and the thickness of the 4th lumbar multifidus under ultrasound at 6 and 12 weeks, using musculoskeletal ultrasound (MU)-guided needle-knife loosening therapy for treating refractory nonspecific low back pain (RNSLBP) compared to usual care, to demonstrate the clinical efficacy of needle knife therapy for RNSLBP. This study used a single-blind, randomized controlled design.

Methods: A total of 66 patients with RNSLBP who met the inclusion criteria were randomly divided into an observation group and a control group of 33 patients.

Endometrial polyps with bizarre stromal cells: a Benign or a low-grade lesion?

Background: Polyps containing bizarre stromal cells are occasionally observed in the lower gynecologic tract, including the vagina, cervix, and endometrium, predominantly in perimenopausal or postmenopausal patients. These cases have traditionally been considered benign without subsequent recurrence or malignancy.

Case Presentation: We describe a rare instance of a rapid enlarging endometrial polyp characterized by atypical stromal cells in a 76-year-old postmenopausal woman, who presented with vaginal bleeding.

The diagnostic value of cytology in the mammary intraductal lesions of patients with pathological nipple discharge.

This study aimed to explore the diagnostic value of the two cytology techniques, including liquid-based cytology of mammary ductal lavage fluid and nipple discharge smear cytology, in the intraductal lesions in patients with pathological nipple discharge (PND). This retrospective analysis included 119 patients with PND who underwent surgical treatment. At the same time, they all underwent fiberoptic ductoscopy (FDS), nipple discharge smear cytology and liquid-based cytology of ductal lavage fluid before surgery.

Direct Observation of Substantial Phonon Nonequilibrium Near Nanoscale Hotspots in Gallium Nitride.

Phonon modal nonequilibrium is believed to widely exist around nanoscale hotspots, which can significantly affect the performance of nano-electronic and optoelectronic devices. However, such a phenomenon has not been explicitly observed in 3D device semiconductors at the nanoscale. Here, by employing a tip-enhanced Raman thermal measurement approach, substantial phonon nonequilibrium in gallium nitride near sub-10 nm laser-excited hotspots is directly revealed for the first time.

Targeting CD45 by gene-edited CAR T cells for leukemia eradication and hematopoietic stem cell transplantation preconditioning.

Hematopoietic stem cell transplantation (HSCT) is widely used to treat patients with life-threatening hematologic and immune system disorders. Current nontargeted chemo-/radiotherapy conditioning regimens cause tissue injury and induce an array of immediate and delayed adverse effects, limiting the application of this life-saving treatment. The growing demand to replace canonical conditioning regimens has led to the development of alternative approaches, such as antibody-drug conjugates, naked antibodies, and CAR T cells.