Multicenter Practice of Non/Minimized Fluoroscopy Ablation for Paroxysmal AF in China: The PAF-ICE Trial.

Background: Intracardiac echocardiography (ICE)-guided non/minimized-fluoroscopy catheter ablation for atrial fibrillation (AF) has been reported, but its effectiveness and safety still lack multicenter evidence.

Objectives: The authors sought to evaluate the effectiveness and safety of ICE-guided non/minimized-fluoroscopy catheter ablation compared with the traditional fluoroscopy-guided approach in patients with paroxysmal AF.

Methods: A total of 448 patients with paroxysmal AF, from 15 centers in China, were randomly assigned in a 1:1 ratio to a non/minimized-fluoroscopy group (n = 223) and a traditional approach group (n = 225).

High-Performance Thick-Film All-Polymer Solar Cells Enabled by a Blade-Coating Process Assisted by a Direct Nonuniform Electric Field.

The incorporation of thick active layers (>300 nm) is an essential requirement for wide-scale industrial production of organic solar cells (OSCs). However, it is still challenging to achieve efficient thick film devices, in particular for all-polymer OSCs, which are generally considered the most stable type of OSCs. In this study, a simple yet effective method is introduced by using a direct current (DC) field to manipulate the morphology of bulk heterojunction (BHJ) films within all-polymer OSCs during a blade coating process.

Brucella-infected macrophage-derived Exosomes enhance the anti-Brucella acquired immune response.

In recent years, the roles of Exosomes in tumors and infectious diseases have been found to have potential application value in disease diagnosis and treatment. However, whether Exosomes are involved in host-acquired immune responses against Brucella has not been reported. This study explored the significance of Exosomes in the context of Brucella infection and their influence on host immune responses.

Environmental Microbiome Characteristics and Disinfection Strategy Optimization in Intensive Dairy Farms: Bactericidal Efficacy of Glutaraldehyde-Based Combination Disinfectants and Regulation of Gut Microbiota.

As the primary biological risk threatening safe dairy production, bovine mastitis control highly relies on environmental disinfection measures. However, the mechanisms by which chemical disinfectants influence host-environment microbial interactions remain unclear. This study systematically investigated the disinfection efficacy and regulatory effects on microbial community composition and diversity of glutaraldehyde-benzalkonium chloride (BAC) and glutaraldehyde-didecyl dimethyl ammonium bromide (DAB) at recommended concentrations (2-5%), using 80 environmental samples from intensive dairy farms in Xinjiang, China.

Nano-enabled delivery of anesthetics: mechanistic insights, technological advances and translational challenges.

The application of anesthetics constitutes a cornerstone of modern medicine, yet conventional agents are plagued by suboptimal pharmacokinetics and potential toxicity. Nanotechnology has emerged as a transformative paradigm, facilitating the design of sophisticated carriers that enable unprecedented precision in anesthetic delivery. This narrative review provides a critical synthesis of the field, advancing beyond conventional material cataloguing to deliver a comprehensive "bench-to-bedside" analysis.

Synergistic surface doping of organic crystals with source-gated architectures: ultra-stable, high-mobility and strain-insensitive stretchable transistors.

Despite remarkable advancements in organic flexible electronics, performance variability and operational instability, rooted in weak van der Waals interaction correlated defects, remain long-standing challenges. Herein, we address these issues through a synergistic strategy integrating organic single crystals, surface doping, and source-gated transistors (SGTs). FTS ((tridecafluoro-1,1,2,2-tetrahydrooctyl)trichlorosilane) monolayer decoration contributes to a trap-free and high-conductance (11.

Primary Intrahepatic Biliary Neuroendocrine Neoplasms: A Case Report and Literature Review.

Introduction: Intrahepatic biliary neuroendocrine neoplasms (NENs) are rare neoplasms originating from neuroendocrine cells in the intrahepatic bile ducts. Patients often present without hormone-related symptoms but are admitted due to jaundice or abdominal pain.

Presentation Of Case: This case report describes a 57-year-old female patient who experienced intermittent upper abdominal pain for one month prior to admission.

Hierarchically porous UiO-67-bpy metal-organic gels for efficient tetracycline removal.

The existence of TC residues in the environment and the resultant development of opposition mechanisms constitute a serious menace to both ecological systems and human health. In the present study, UiO-67-bpy-X gels were prepared by the introduction of Hbpydc ligands and acetic acid modifiers, followed by a systematic evaluation of their TC adsorption capabilities. Characterization results revealed that UiO-67-bpy-2.

Impact of surgical care pathways on efficiency and outcomes in orthopedic operating rooms: a historical control study.

Objective: The impact of surgical care pathways (CP) on efficiency and patient outcomes in orthopedic operating rooms (OR) is unclear, and we aim to optimize nursing strategies and enhance service quality.

Methods: From July 2019 to June 2024, 3,836 patients undergoing orthopedic surgery at a single Grade 3 Chinese hospital were retrospectively analyzed and divided into two equal groups, with or without CP (1,918 patients in each group). The effectiveness of CP was assessed by comparing surgical site infection (SSI) rates, pathogens, OR turnover times, and patient satisfaction.

Emerging trends and global collaboration in Paclitaxel resistance research for breast cancer: a comprehensive bibliometric study.

Paclitaxel, a compound that targets microtubules in cancer cells and inhibits mitosis, has been used as a first-line treatment for recurrent or metastatic breast cancer for over three decades. Research into paclitaxel resistance has yielded thousands of articles. However, this body of research has not been analyzed systematically and quantitatively.

Utilizing the synergistic biophysical and biogeochemical processes to estimate the impacts of forest landscape characteristics on land surface temperature and carbon stock in southern China.

Forest landscape degradation leads to the deterioration of ecological processes and adverse climate impacts. Although individual biophysical (BPH) and biogeochemical (BGC) effects of forest change are relatively well-studied, their synergistic interactions and combined influences of the forest landscape on climate remain complex and not fully understood. The potential impact of forest landscape patterns on land surface temperature and carbon stock using highly reliable satellite observations and climate models was assessed.

Sunlight-driven photoinduced electron/energy transfer-reversible addition-fragmentation chain transfer polymerization at a large scale.

Photoinduced electron/energy transfer-reversible addition-fragmentation chain transfer (PET-RAFT) polymerization is a sustainable and powerful tool to synthesize polymers and copolymers. However, the development of large-scale production and polymerizations using sunlight irradiation remain top challenges for PET-RAFT polymerizations. In this study, conjugated cross-linked phosphine (PPh-CHCP) was explored as a heterogeneous photocatalyst for efficient PET-RAFT polymerization.

Multi-loop Balanced Continuous Suture for the Treatment of Radial Tears in the Body of the Meniscus.

Radial tears in the body of the meniscus occur quite often in clinical practice. Because of the concentration of stress, lack of resistance to external forces, and poor blood supply in this area, healing is more difficult compared with other types of tears. As a result, some physicians may perform meniscectomy, which can lead to rapid joint degeneration and osteoarthritis.

Arthroscopic Repair of Posterior Cruciate Ligament Tibial Avulsion Fractures Using a "Y"-Shaped Suture Technique Combined With Suspension Fixation.

The posterior cruciate ligament (PCL) plays a crucial role for the posterior stability of the knee joint, accounting for 3% to 38% of acute knee injuries and frequently co-occurring with other ligament injuries. PCL tibial avulsion fractures are not rare in clinical practice. Arthroscopic treatment for PCL tibial avulsion fractures has gained acceptance due to its ability to achieve anatomic reduction, restore ligament tension, and improve joint stability while minimizing surgical trauma and facilitating rapid recovery.

Small is better: Impact of polyelectrolyte Mw on layer-by-layer nanofiltration membranes for PFAS removal.

For removal of linear and negative charged Per- and polyfluoroalkyl substances (PFAS), highly negatively charged and small pore NF membranes are urgently needed. This work reports, for the first time, an attempt to regulate the pore size and surface charge of polyvinylamine/sodium polystyrene sulfonate (PVAm/PSS) layer-by-layer nanofiltration (LBL NF) membranes by varying the polyelectrolytes' molecular weights (Mw) for PFAS removal. In general, PVAm/PSS NF membranes exhibited a strong negative surface charge, with 99.

Unveiling B7/CD28 family proteins in hepatocellular carcinoma: insights into communication and prognostic significance.

Background: Immunotherapy has made remarkable achievements in cancer treatment, but it still faces the challenge of limited response rates in liver cancer therapy. Although there has been extensive research on the role of programmed cell death-ligand 1 (PD-L1) in hepatocellular carcinoma (HCC), our understanding of the effects of other inhibitory B7/CD28 family members is still limited despite advancements in prognostic tools, more specific, accurate, and robust biomarkers are required to improve HCC patient prognoses.

Methods: We acquired the single-cell sequencing data from relevant literature and selected 42 liver tissue samples, including 89,246 cells from HCC patients, to investigate the expression, localization, and intercellular communications of the B7/CD28 family in HCC.

METTL1-WDR4 promotes the migration and proliferation of gastric cancer through N-methylguanosine.

Background: Gastric cancer (GC) is one of the most common malignant tumor worldwide. Metastasis is leading cases of cancer-related death of GC. It has been found that N-methylguanosine (m7G) modifications play an important role in cancer.

Hofmeister effect of anions on the layer-by-layer nanofiltration membranes: Assembly kinetics and micropollutant removal.

The impact of anions on the assembly process, subsequent physicochemical characteristics and separation performance of layer-by-layer (LBL) nanofiltration (NF) membranes has not been fully elucidated. In this study, model polyelectrolyte (PE) pairs of poly(sodium 4-styrenesulfonate) (PSS) and poly(diallyldimethylammonium chloride) (PDADMAC) were chosen and monovalent anions in the Hofmeister series-kosmotropes (F, Ac), Cl, chaotropes (Br, NO)-were paired with Na as the background salt. Membranes from chaotropic anions had narrow distributions of small pores, relatively low permeance and nearly electroneutral, even slightly electropositive separation layer; fewer coating bilayers were required to reach the plateau in MgSO rejection, corresponding to faster PE adsorption and thicker coating.

Acute effects of mercury on brine shrimp: First evidence highlighting the molecular mechanism through integrated transcriptome and metabolomic analysis.

Mercury poses significant hazards to aquatic organisms, causing neurotoxicity, and metabolic disorders. Research on the dynamic distribution of mercury within zooplankton is limited, and the metabolic mechanisms of mercury in zooplankton remain unclear. In this study, we employed a mercury-specific fluorescent probe (aggregation-induced emission luminogen, AIEgen) to visualize the in vivo distribution of Hg and MeHg in brine shrimp.

Isolation of largemouth bass virus (LMBV) and dominant immunogenic epitope screening of the major capsid protein of LMBV.

Increasing disease challenges represent a critical bottleneck for the sustainable development of largemouth bass farming. Vaccination can effectively protect fish from pathogens, with dominant immunogenic epitope identification being essential for vaccine development. This research documents a case of largemouth bass virus (LMBV) infection and identifies the dominant immunogenic epitope of the major capsid protein of LMBV (LMBV-MCP).

Single-Cell Sequencing Identifies the Crucial Role of Mitochondrial Fission-Fusion Imbalance in Heart Failure Progression.

The heart grows in response to both pathological and physiological stimuli. Pathological hypertrophy often leads to cardiomyocyte loss and heart failure (HF), whereas physiological hypertrophy paradoxically protects the heart. Comparing these two types of hypertrophy can elucidate the differences and connections in their molecular mechanisms, which is pivotal for unraveling the pathogenesis of HF.

PepTCR-Net: prediction of multi-class antigen peptides by T-cell receptor sequences with deep learning.

Predicting T-cell receptor (TCR) recognizing antigen peptides is crucial for understanding the immune system and developing new treatments for cancer, infectious and autoimmune diseases. As experimental methods for identifying TCR-antigen recognition are expensive and time-consuming, machine-learning approaches are increasingly used. However, existing computational tools often struggle with generalization due to limited data and challenges in acquiring true non-recognition pairs and rarely integrate multiple biological features into unified frameworks.

Recapitulation of angiogenesis and osteogenesis within an muscle pouch-based coral-derived macroporous construct organoid model.

Background: Segmental bone defect is a challenging clinical problem that often requires autologous bone grafting, which has limitations such as donor site morbidity and insufficient supply. Bone tissue engineering aims to create functional bone substitutes that can mimic the properties and processes of native bone. However, the discrepancy between and conditions hinders the successful translation of bone tissue engineering from animal models to human applications.

Interaction of Layer-by-Layer Nanofiltration Membranes and PFAS: Filtration after Adsorption.

Removal of perfluoroalkyl and polyfluoroalkyl substances (PFAS) by nanofiltration (NF) membranes has been well-documented, but the impact of PFAS interaction with the NF separation layer on the permselectivity of layer-by-layer (LBL) NF membranes has yet to be explored. The work reported two different LBL membranes prepared with pairs of long-chain polyelectrolytes (PVAm/PSS, L/L) and short-chain polyelectrolytes (S/S) for analysis of the impact of PFAS adsorption on removal. Both L/L and S/S membranes showed high NaSO rejection (99.