Sunlight-driven photoinduced electron/energy transfer-reversible addition-fragmentation chain transfer polymerization at a large scale.

Photoinduced electron/energy transfer-reversible addition-fragmentation chain transfer (PET-RAFT) polymerization is a sustainable and powerful tool to synthesize polymers and copolymers. However, the development of large-scale production and polymerizations using sunlight irradiation remain top challenges for PET-RAFT polymerizations. In this study, conjugated cross-linked phosphine (PPh-CHCP) was explored as a heterogeneous photocatalyst for efficient PET-RAFT polymerization.

[Corrigendum) RNA interference‑mediated FANCF silencing sensitizes OVCAR3 ovarian cancer cells to adriamycin through increased adriamycin‑induced apoptosis dependent on JNK activation.

After the publication of the article, an interested reader drew to the authors' attention that there appeared to be a pair of overlapping data panels in Fig. 4C on p. 1726 [specifically, the 'Untransfected' and 'Control shRNA' data panels for the ADM (24 h) experiments].

5-Aza-2-deoxycytidine alleviates the progression of primary biliary cholangitis by suppressing the FoxP3 methylation and promoting the Treg/Th17 balance.

Primary biliary cholangitis (PBC) is a common autoimmune liver disease manifested by the infiltration of CD4 T cells, and the subsequent targeted injury of biliary epithelial cells (BECs). As important components of CD4 subsets, the Treg/Th17 axis maintains an immunological balance between self-tolerance and inflammation in the liver microenvironment. However, the role and regulatory mechanism of the Treg/Th17 axis in PBC remain unclear.

RNA interference-mediated FANCF silencing sensitizes OVCAR3 ovarian cancer cells to adriamycin through increased adriamycin-induced apoptosis dependent on JNK activation.

In the present study, we downregulated FANCF expression by small interfering RNA (siRNA) in OVCAR ovarian cancer cells to address the effects of decreased FANCF expression on the function of the Fanconi anemia (FA)/breast cancer susceptibility gene (BRCA) pathway. Furthermore, we investigated whether this method increases the sensitivity of OVCAR3 cells to adriamycin (ADM) and the possible mechanism(s). We found that silencing of FANCF inactivated the FA/BRCA pathway by decreasing the monoubiquitination and focus formation of FANCD2 and reduced the function of the FA/BRCA pathway, resulting in the inhibition of cell proliferation, increased cell apoptosis and DNA damage in OVCAR3 cells.

[Effect of troxerutin and cerebroproptein hydrolysate injection on platelet aggregation and thrombosis].

This study was purposed to explore the effect of troxerotin and cerebroproptein hydrolysate injection (TCHI) on platelet aggregation in vitro and thrombosis in vivo. The inhibitory rate of TCHI at different concentrations on platelet aggregation was determined by platelet aggregometer. The relationship between dose and effect was established.

Distribution of HLA alleles and haplotypes in Jinuo and Wa populations in Southwest China.

The frequencies of human leukocyte antigen (HLA) alleles HLA-A, -B, and -DRB1 alleles and A-B-DRB1, A-B, and B-DRB1 haplotypes were studied in Jinuo and Wa populations in Southwest China using the polymerase chain reaction-Luminex (PCR-Luminex) typing method. A total of 12 A, 22 B, and 16 DRB1 alleles were found in the Jinuo population, and 10 A, 28 B, and 18 DRB1 alleles were found in the Wa population. The A*110101-B*1502-DRB1*120201 was the predominant haplotype in both the Jinuo and Wa populations; A*110101-B*1301-DRB1*120201 and A*24020101-B*1502-DRB1*120201 were common in the Jinuo population, whereas A*110101-B*1532-DRB1*1504 and A*110101-B*350101-DRB1*1404 were common in the Wa population.

[Collection of a Chinese pedigree with Parkinson's disease and linkage analysis of nine susceptibility genes].

Objective: To analyze the susceptibility genes of a Parkinson's Disease (PD) family.

Methods: The blood samples of a four-generation classic idiopathic PD family were collected. Two-point LOD score method was applied to analyze the linkage disequilibrium between the disease locus and microsatellite markers.

[Polymorphism of DYS287 on Y chromosome in 28 ethnic populations of China].

Objective: To investigate the polymorphism of DYS287 among 28 ethnic populations in 9 provinces of China.

Method: YAP element was detected by Touchdown PCR amplification and 2% agarose gel electrophoresis.

Results: YAP+ frequencies in these ethnic populations were as follows: Zang 36.

[Polymorphism research on the distribution of four HLA alleles in five Chinese populations].

ARMS-SSP PCR was applied to survey the distribution of four AIDS associated HLA alleles, HLA-A*02, B*35, B*27, and B*57 in five Chinese nationalities (Yunnan han, yi, dai, Xinjiang uygur, and Guangxi zhuang). The results showed that HLA-A*02 allele occured very frequently at higher frequencies in han and zhuang than in the other three nationalities; B*35 did not show great variation in it's distribution in five population groups; B*27 had a distinctively high frequency in dai, but normal in han, yi and zhuang; B*57 allele did not vary greatly in these nationalities, too. A*02, B*27 and B*57 are three protective alleles.

[Gene frequencies and haplotypes of nine Y-short tandem repeat loci in four minority populations of China].

Objective: To investigate the polymorphism of nine Y-short tandem repeat loci in 117 male individuals from four minority populations of China, and to obtain the polymorphism information in these populations.

Methods: Nine loci in all samples were amplified by PCR. Products were electrophoresed on ABI PRISM 377 DNA sequencer.

Regulation of alternative splicing of Bcl-x by IL-6, GM-CSF and TPA.

The splicing of many alternative exons in the precursor messenger RNA (pre-mRNA) is regulated by extracellular factors but the underlying molecular bases remain unclear. Here we report the differential regulation of Bcl-x pre-mRNA splicing by extracellular factors and their distinct requirements for pre-mRNA elements. In K562 leukemia cells, treatment with interleukin-6 (IL-6) or granulocyte-macrophage colony stimulating factor (GM-CSF) reduced the proportion of the Bcl-xL variant mRNA while treatment with 12-O-tetradecanoylphorbol 13-acetate (TPA) had no effect.

[Genetic analysis of 17 biallelic markers on Y chromosome in 3 Chinese ethnic group populations].

Objective: To study the genetic polymorphism of Y chromosome in different Chinese ethnic group populations.

Methods: Genotypes of 17 biallelic markers located in the nonrecombining portion of the Y chromosome in 76 men from 3 Chinese ethnic group populations (Han in Shandong, Bai in Yunnan, and Tu in Qinghai) were examined with polymerase chain reaction (PCR) and allelic-specific PCR (ASPCR). Their haplotypes made of these 17 binary markers were constructed.

[Regulation of pre-mRNA alternative spicing in U251 cells by TPA].

By using the mini-gene construct containing partial sequence of Bcl-X gene as model, we examined the function of TPA on Bcl-X pre-mRNA alternative splicing in vivo and vitro with RT-PCR and site-directed mutagesis assay. The results show that PKA signaling system can regulate Bcl-X pre-mRNA alternative splicing, the possible mechanism is that the responsible sequence affect the choice of the 5'-downstream or upstream splice site of Bcl-X pre-mRNA.

[Immunogenecity of combined hepatitis A and B vaccine].

Objective: To observe the immunogenicity of combined hepatitis A and B vaccine (HAB).

Methods: The combined HAB vaccine was prepared and different concentrations of HAB were administered on mice in week 0, 4 and 24, and then we tested the antibodies to both hepatitis A virus and B virus. After the first injection, we tested the hepatitis A antigen-induced and hepatitis B surface antigen-induced stimulation indices in spleen monocyte as well as changes of CD4+ and CD8+ cell numbers.

[Genotyping of HLA-DRB1 by PCR-SSP in Yunnan Lahu ethnic groups].

Objective: To investigate polymorphism of HLA-DRB1 in Chinese Lahu population in Yunnan.

Methods: Polymerase chain reaction-sequence specific primers (PCR-SSP) were used to determine HLA-DRB1 genotypes of 110 unrelated healthy Lahu minority people of Yunnan Province.

Results: Sixteen alleles of DRB1 were detected in this study.