Analysis of lncRNA-mRNA pairs induced by Colletotrichum camelliae reveals Cslnc170 as a regulator of CsLOX4 in tea plants.

Fungal diseases such as anthracnose substantially affect the growth of tea (Camellia sinensis) plants. Understanding disease resistance mechanisms and identifying resistance genes will aid in breeding resistant varieties. Non-coding RNAs, including long non-coding RNAs (lncRNAs), play critical roles in regulating plant immunity by influencing target gene expression; however, their role in disease resistance of tea plants remains underexplored.

Serine/Threonine Kinase 33 as a Novel Target of Bufalin in Treatment of Triple-Negative Breast Cancer.

Identifying novel therapeutic targets and drugs is crucial for treating triple-negative breast cancer (TNBC). Bufalin, a key active ingredient of the traditional Chinese medicine HuaChansu, has been employed in tumor therapy. Here, SPR-LC-MS/MS is employed to characterize the targets of Bufalin and found that serine/threonine kinase 33 (STK33) possesses a strong binding affinity to Bufalin.

Diagnosis and treatment of a patient with severe hepatitis and rash caused by toripalimab: A case report.

Rationale: Immune-related adverse events have been frequently reported using immune checkpoint inhibitors. However, these reports are commonly observed in single-organ toxicity. Thus, the compound, multiple, multisite, and multi-organ toxicities are seldom recognized and lack treatment experience, finally leading to severe challenges in the clinical treatment of such patients.

Extracellular vesicle proteomic expression is influenced by mining tenure in former uranium miners.

Background: Chronic exposure to uranium (U) rich environments poses significant health risks, yet the molecular mechanisms underlying these effects remain poorly understood. Extracellular vesicles (EVs) are membrane-bound vesicles that transfer multiple biomolecules between cells and can regulate cellular function.

Objective: To determine whether U-mining tenure is associated with specific alterations in serum-derived EV proteomic and plasma cytokine profiles among former U-miners, and to assess the potential of EV-derived proteins as robust biomarkers of chronic U-exposure relative to canonical cytokines.

Linoleic acid drives pulmonary lymphoepithelioma-like carcinoma progression via PPAR-α/TF axis.

Background: Primary pulmonary lymphoepithelioma-like carcinoma (pLELC) is a rare subtype of non-small cell lung cancer(NSCLC) with unclear etiological mechanisms. This study aimed to investigate the underlying molecular mechanisms and therapeutic targets for pLELC.

Methods: Retrospectively collected samples from advanced pLELC patients underwent proteomic and metabolomic analyses, and patient-derived xenograft (PDX) models were established for validation.

Deciphering Mutational Effects on Inducible NO Synthase Conformational Dynamics via Quantitative Cross-Linking Mass Spectrometry and AlphaFold2 Subsampling.

Mammalian nitric oxide synthases (NOSs) are flavo-hemoproteins that rely on dynamic interdomain interactions for activity. Calmodulin (CaM) facilitates specific, interdomain FMN-heme interactions that enable inter-subunit FMN-heme electron transfer essential for nitric oxide biosynthesis. Our quantitative cross-linking mass spectrometry (qXL MS) results demonstrate that the abundance of inter-subunit cross-links correlates with CaM-induced formation of the docked FMN/heme complex in neuronal NOS [Jiang et al.

Harnessing artificial intelligence to identify Bufalin as a molecular glue degrader of estrogen receptor alpha.

Target identification in natural products plays a critical role in the development of innovative drugs. Bufalin, a compound derived from traditional medicines, has shown promising anti-cancer activity; however, its precise molecular mechanism of action remains unclear. Here, we employ artificial intelligence, molecular docking, and molecular dynamics simulations to elucidate the molecular mechanism of Bufalin.

Mapping the Evolution of Thyroid Ultrasound Research: A 30-Year Bibliometric Analysis.

Introduction: Thyroid ultrasound has emerged as a critical diagnostic modality, attracting substantial research attention. This bibliometric analysis systematically maps the 30-year evolution of thyroid ultrasound research to identify developmental trends, research hotspots, and emerging frontiers.

Methods: English-language articles and reviews (1994-2023) from Web of Science Core Collection were extracted.

Evolutionary characterization and pathogenicity of Senecavirus isolated from Shandong, China.

Senecavirus A (SVA), previously called Seneca Valley virus, belongs to the family Picornaviridae, species Senecavirus A, in the Senecavirus genus. SVA infection causes vesicular lesions in sows and a sharp drop in neonatal piglet production. In this study, an SVA strain, SVA/SD/2023, was isolated from a pig farm in North China, and its genome was determined and analyzed.

Emulsifying Properties of Oat Protein/Casein Complex Prepared Using Atmospheric Cold Plasma with pH Shifting.

An oat protein isolate is an ideal raw material for producing a wide range of plant-based products. However, oat protein exhibits weak functional properties, particularly in emulsification. Casein-based ingredients are commonly employed to enhance emulsifying properties as a general practice in the food industry.

Relationship between prenatal anxiety, depression, pregnancy stress and their social determinants.

Background: Prenatal anxiety, prenatal depression, and pregnancy-related stress are prevalent among pregnant women. Across China, the prevalence rates of antenatal depression, anxiety, and pregnancy-related stress are 12.07-36.

College students' cognition and attitude toward medical knowledge education after a global public health event.

Introduction: The past global public health event has heightened governmental and societal awareness of the importance of health and medical knowledge education. This study aims to investigate and compare the attitude and cognition of non-medical students and medical students toward medical knowledge education.

Methods: A self-administered, anonymous questionnaire was voluntarily completed by 304 university students recruited through the Wen-Juan-Xing online platform via WeChat QR codes or website links.

METTL5 deficiency induces oligoasthenoteratozoospermia via impaired 18S rRNA mA methylation in humans and mice.

Recent studies have highlighted RNA modifications as integral regulators of gene expression during spermatogenesis. Ribosomal RNAs (rRNAs) are the most abundant RNA in cells, while the function and clinical relevance of rRNA modifications in spermatogenesis remain poorly understood. Here, we identified 4 pathogenic heterozygous variants of METTL5 in 1,427 patients with male infertility characterized as oligoasthenoteratozoospermia (OAT).

Gastrointestinal tolerance enhancement of the LPxTG-motif surface protein overexpressed Lactobacillus reuteri SH23 in vivo.

Background: Lactobacillus reuteri is a well-recognized probiotic with numerous health benefits, including modulation of gut microbiota, anti-inflammatory effects, and immune system enhancement. However, its probiotic efficacy depends largely on survival through the gastrointestinal tract and effective colonization of the gut mucosa. Surface-anchored LPxTG-motif proteins play crucial roles in bacterial adhesion and enhancing stress resistance.

The cognitive impacts of large language model interactions on problem solving and decision making using EEG analysis.

Introduction: The increasing integration of large language models (LLMs) into human-AI collaboration necessitates a deeper understanding of their cognitive impacts on users. Traditional evaluation methods have primarily focused on task performance, overlooking the underlying neural dynamics during interaction.

Methods: In this study, we introduce a novel framework that leverages electroencephalography (EEG) signals to assess how LLM interactions affect cognitive processes such as attention, cognitive load, and decision-making.

Angiogenic apoptotic vesicle-laden silk fibroin /sodium alginate hydrogel for pulp regeneration.

Angiogenesis remains a key challenge in pulp regeneration due to the ischemic and hypoxic root canal environment. Apoptotic vesicles (ApoVs) offer unique advantages as a breakthrough strategy due to their stability in hypoxic environments, resistance to immune clearance, and high yield. However, a critical gap persists in biomaterials capable of spatiotemporal ApoVs delivery for pulp regeneration.

Single-cell RNA sequencing uncovers abnormal Sertoli-cell elevation and testicular niche impairment in the transfemales's testis.

Background: Transgender women (transfemales) often undergo gender-affirming hormone therapy (GAHT). However, the testicular impacts of feminising hormones present heterogeneity due to the complexity of testicular regulatory mechanisms.

Method: In this study, we analyzed approximately 49,385 single-cell transcriptomes from transfemale human testicular tissue, comparing cellular composition with that of cisgender male individuals across a range of ages.

The V-ATPase a3 subunit deficiency affects osteoarthritis via mTOR-Mediated autophagy levels in subchondral bone.

Autophagy is an evolutionarily conserved cellular self-degradation process that eliminates damaged organelles and misfolded proteins, thereby maintaining cellular homeostasis and delaying apoptosis and tissue degeneration. The efficient progression of autophagy depends on the maintenance of intracellular homeostasis, in which vacuolar ATPases (V-ATPases) play a crucial role by facilitating lysosomal acidification. Among these, the a3 subunit of V-ATPase, encoded by the T-cell immune regulator 1 (TCIRG1, also known as ATP6V0a3), is highly expressed in osteoclasts.

Ionizing radiation-mediated dendritic cell maturation exacerbates inflammatory response of bone marrow mesenchymal stem cells and impairs osteogenesis in radiation-induced jaw injury.

Background: Radiation-induced jaw injury is one of the most severe complications after radiotherapy for head and neck cancer, which can disrupt patients' health and quality of life. Although the direct target of inflammation and suppressed bone regeneration activity by ionizing radiation (IR) has been phenomenally observed, the underlying mechanisms and potential therapeutic targets remain blurred. Osteoimmunology emphasizes that dendritic cells (DCs) may contribute to bone diseases.

Selective blockade of acid-sensing ion channel 1a can provide substantial hippocampal neuroprotection.

Background: Acid-sensing ion channel 1a (ASIC1a) is the only member of the ASIC family where Ca osmosis has been reported, and it is highly expressed in neurons of the central nervous system. This study aimed to investigate whether ASIC1a is trafficked to the plasma membrane and regulated by the Rho/ROCK and PI3K signaling pathways in temporal lobe epilepsy (TLE). In addition, further research is required to determine whether selective ASIC1a blockade is a viable therapeutic strategy for TLE.

Role of the renin-angiotensin-aldosterone system in sepsis and its therapeutic targets.

An imbalance in the host's response to infection causes sepsis, a potentially fatal condition. Sepsis may advance to septic shock, organ dysfunction (including the brain, liver, lung, kidney, and heart), skeletal muscle atrophy, and an array of severe complications. The renin-angiotensin-aldosterone system (RAAS) undergoes significant alterations during sepsis, and the expression of its principal bioactive components is closely associated with the prognosis of patients with sepsis.

Study on Superhydrophilic Surface Treatment Methods and Magnified Preparation for Stainless Steel.

This study proposes an environmentally benign electrochemical method for fabricating superhydrophilic surfaces on stainless steel, enabling dual-functional modification through both anodic oxidation and cathodic reduction. By employing citric acid and ammonium chloride as noncorrosive electrolytes, milli-microscale porous morphologies were formed on anodically treated surfaces, while hydrophilic metallic coatings developed on cathodically treated surfaces, both achieving superhydrophilicity with water contact angles <10°. The critical role of initial current density in determining pore distribution density on anodic surfaces was systematically investigated, revealing that pore formation is governed by the "tip effect"─localized charge accumulation at pre-existing pore edges drives preferential reactions, independent of subsequent current adjustments.

Enhancing inter-system crossing efficiency of NIR-II emitting type-I photosensitizers for tumor ferroptosis induction.

Ferroptosis, an iron-dependent form of regulated cell death driven by lipid peroxidation (LPO), represents a compelling avenue for cancer therapy. Photodynamic therapy (PDT), which relies on the production of LPO-reactive oxygen species (ROS), has emerged as a potent therapeutic strategy. However, the clinical efficacy of conventional photosensitizers (PSs) is frequently constrained by tumor hypoxia, which intensifies local oxygen deficiency and diminishes PDT performance.

AAAP gene family evolution and transcriptional regulation in Eucalyptus grandis under nitrogen, phosphate and boron deficiencies.

Background: is one of the most important fast-growing tree species in the world, and its growth and development are significantly affected by nitrogen and phosphorus. The Amino acid/auxin permease (AAAP) gene family plays key roles in long-distance amino acid transport in plants, but their evolutionary diversity and gene expression analysis in under nutrient deficiency stress are largely unexplored.

Results: This study presents the first genome-wide identification and functional characterization of 78 AAAP family genes (EgAAAPs) in , classified into eight subfamilies.