Safety, Tolerability, and Immunogenicity of a Recombinant Nonavalent Human Papillomavirus Vaccine () in Healthy Chinese Women Aged 18-45 Years: A Phase 1 Clinical Trial.

Background: Prophylactic human papillomavirus (HPV) vaccination substantially alleviates cervical cancer burden. This study aimed to evaluate the safety, tolerability, and immunogenicity of an -expressed recombinant nonavalent HPV vaccine.

Methods: A dose-escalating phase 1 clinical trial was conducted in Sheyang County, Jiangsu Province, China.

Identification and Verification of the Driver Genes for the Formation and Development of Intracranial Aneurysms.

Background: Research on driver genes that can be used to diagnose and control the formation and development of intracranial aneurysms (IAs) is still limited, and bioinformatics and machine learning approaches are implemented in the study in an aim to identify and validate them.

Methods: By applying datasets from the Gene Expression Omnibus database for human cerebrovascular tissue, 47 cases of ruptured IA, 71 cases of ruptured IA, and 64 cases of normal control intracranial vessels were analyzed. Apply bioinformatics and machine learning methods to screen for the driver genes that contribute to the occurrence and development of IAs.

Stability study of recombinant 9-valent human papillomavirus vaccine based on expression system.

This study reports on the long-term stability of a recombinant 9-valent HPV vaccine, addressing a gap in the literature as previous research did not extend beyond 72 months. The vaccine targets HPV types 6, 11, 16, 18, 31, 33, 45, 52, and 58 and was produced using an expression system. We optimized soluble HPV L1 protein expression by truncating the - and C-termini, resulting in HPV L1 virus-like particles (VLPs).

Fused Triazole-Tetrazine Assembled with Different Functional Moieties: Construction of Multipurpose Energetic Materials.

Azido, amino, and azo functionalities were introduced into tetrazine backbones to access multifunctional energetic materials. demonstrates effective initiation capability (MPC = 50 mg), whereas balances well between the energy and stability. Azo-functionalized has a high density of 1.

Unravelling the history of mountain belts through U-Pb and Lu-Hf dating of zircon and Ar/Ar dating of detrital white mica: a case study from the Eastern Alps.

Radiogenic isotopes of igneous and detrital minerals from various clastic rocks of mountain belts are used to reveal tectonic and sedimentary processes, which are otherwise difficult to detect. Here, we discuss the results of U-Pb and Lu-Hf zircon systems, and Ar/Ar on detrital white mica in Eastern Alps. Zircon and white mica are chemically and mechanically stable and occur in magmatic, metamorphic and sedimentary rocks.

Transcriptome analysis provides insights into coumarin biosynthesis in the medicinal plant Angelica dahurica cv. Yubaizhi.

Angelica dahurica roots have a long history of use in traditional Chinese medicine due to their high coumarin content. To address the increasing demand for these roots, a synthetic biology approach has been proposed. Nevertheless, our comprehension of coumarin biosynthesis and its regulation remains limited.

Metabolic characteristics of self-pollinated wheat seed under red and blue light during early development.

Blue light has a greater effect on jasmonic acid and flavonoid accumulation in wheat seeds than red light; blue light reduces starch synthesis and the size of starch granules and seeds. This study sought to elucidate the effects of blue and red light on seed metabolism to provide important insights regarding the role of light quality in regulating seed growth and development. We used combined multi-omics analysis to investigate the impact of red and blue light (BL) on the induction of secondary metabolite accumulation in the hexaploid wheat Dianmai 3 after pollination.

Immunogenicity and protective effects of recombinant bivalent COVID-19 vaccine in mice and rhesus macaques.

Coronavirus disease (COVID-19) is still spreading rapidly worldwide, and a safe, effective, and cheap vaccine is still required to combat the COVID-19 pandemic. Here, we report a recombinant bivalent COVID-19 vaccine containing the RBD proteins of the prototype strain and beta variant. Immunization studies in mice demonstrated that this bivalent vaccine had far greater immunogenicity than the ZF2001, a marketed monovalent recombinant protein COVID-19 vaccine, and exhibited good immunization effects against the original COVID-19 strain and various variants.

Exploring and applying genes to enhance the resistance to Fusarium head blight in wheat.

Fusarium head blight (FHB) is a destructive disease in wheat worldwide. species complex (FGSC) is the main causal pathogen causing severe damage to wheat with reduction in both grain yield and quality. Additionally, mycotoxins produced by the FHB pathogens are hazardous to the health of human and livestock.

Integrating transcriptomics and metabolomics to analyze quinoa () responses to drought stress and rewatering.

The crop production of quinoa (.), the only plant meeting basic human nutritional requirements, is affected by drought stress. To better understand the drought tolerance mechanism of quinoa, we screened the drought-tolerant quinoa genotype "Dianli 129" and studied the seedling leaves of the drought-tolerant quinoa genotype after drought and rewatering treatments using transcriptomics and targeted metabolomics.

Transcriptome and Metabolome Combined to Analyze Quinoa Grain Quality Differences of Different Colors Cultivars.

Quinoa ( Wild.) has attracted considerable attention owing to its unique nutritional, economic, and medicinal values. Meanwhile, quinoa germplasm resources and grain colors are rich and diverse.

Combined transcriptome and metabolome analysis of the resistance mechanism of quinoa seedlings to .

Quinoa has attracted considerable attention owing to its unique nutritional, economic, and medicinal values. The damage intensity of at the seedling stage of quinoa fluctuates with the crop's biological cycle and the environmental changes throughout the growing season. In this study, we used independently selected quinoa seedling resistant and susceptible cultivars to investigate the difference between insect resistance and insect susceptibility of quinoa at the seedling stage.

Development and characterization of anti-HPV16 monoclonal antibodies for assembly of an HPV16 detection kit.

Quality control is very important during the development of 3-valent (16/18/58), 9-valent (6/11/16/18/31/33/45/52/58), and 15-valent human papillomavirus (HPV) vaccines (6/11/16/18/31/33/35/39/45/52/56/58/59/68). All 3-valent, 9-valent, and 15-valent HPV vaccines contain the HPV16 antigen; therefore, a detection method that can specifically identify HPV16 in vaccines is urgently required. This study aimed to develop and characterize monoclonal antibodies to assemble a highly specific HPV16 detection kit.

Development of HPV58 type-specific antibodies and detection kit.

Currently, human papillomavirus (HPV) vaccines are in short supply, so the development of HPV vaccines has a broad market prospect. The 3-, 9-, and 15-valent HPV vaccines developed by ourselves all contain HPV58-derived antigen components. It is important to detect HPV 58 during vaccine production.

Transcriptomics and metabolomics analyses of the mechanism of flavonoid synthesis in seeds of differently colored quinoa strains.

Quinoa (Chenopodium quinoa Willd.) is an herb of the genus Chenopodiaceae that is native to the Andes Mountains of South America. To understand the metabolic differences between various quinoa strains, we selected quinoa strains of four colors (black, red, yellow, and white) and we subjected seeds to extensive targeted metabolomics analysis using liquid chromatography-tandem mass spectrometry and transcriptomics analysis.

Transcriptomics Integrated With Widely Targeted Metabolomics Reveals the Mechanism Underlying Grain Color Formation in Wheat at the Grain-Filling Stage.

Colored wheat grains have a unique nutritional value. To elucidate the color formation mechanism in wheat seeds, comprehensive metabolomic and transcriptomic analyses were conducted on purple (Dianmai 20-1), blue (Dianmai 20-8), and white (Dianmai 16) wheat at the grain-filling stage. The results showed that the flavonoid biosynthesis pathway was closely related to grain color formation.

Non-targeted metabolomics of quinoa seed filling period based on liquid chromatography-mass spectrometry.

Quinoa (Chenopodium quinoa Willd.), an herb belonging to the amaranth family, is rich in minerals, amino acids, vitamins, proteins, and flavonoids. Its grain, compared with other major grains, has unique nutritional value with tremendous applications.

Cross-neutralizing antibody titres against non-vaccine types induced by a recombinant trivalent HPV vaccine (16/18/58) in rhesus macaques.

Human papillomavirus (HPV) causes not only most cervical cancers but also cancers of the vagina, vulva, penis, anus, rectum, and oropharynx. Every year, 200,000 women die of cervical cancer in the world, and China accounts for about 10%. HPV vaccines are effective in preventing HPV infections thus HPV-related cancers worldwide.

Total synthesis of (±)-epi-stemodan-13α,17-diol.

Stemodan-13α,17-diol is a natural stemodane-type diterpenoid isolated from Stemodia chilensis. Herein we report the total synthesis of its epimer, stemodan-13β,17-diol, by applying titanium-mediated polyene cyclization and iron-catalyzed [5 + 2] cycloaddition as the key transformations to expeditiously install the molecular scaffold.

A Novel Human Papillomavirus 16 L1 Pentamer-Loaded Hybrid Particles Vaccine System: Influence of Size on Immune Responses.

Cervical cancer remains the second-most prevalent female malignancy around the world, leading to a great majority of cancer-related mortality that occurs mainly in developing countries. Developing an effective and low-cost vaccine against human papillomavirus (HPV) infection, especially in medically underfunded areas, is urgent. Compared with vaccines based on HPV L1 viruslike particles (VLPs) in the market, recombinant HPV L1 pentamer expressed in Escherichia coli represents a promising and potentially cost-effective vaccine for preventing HPV infection.

Development and characterization of an HPV18 detection kit using two novel HPV18 type-specific monoclonal antibodies.

Background: HPV 18 is one of the most prevalent oncogenic types, only second to HPV 16, and included in the licensed vaccines on the market. In this study, we describe the production and characterization of a panel of monoclonal antibodies (mAb) to HPV18.

Methods: The immunocompetence of 1B1 and 4C2 mAbs for HPV L1 protein was evaluated by SDS-PAGE analysis, neutralization assays, affinity identification, and ELISA.

Iron-Catalyzed Intramolecular Perezone-Type [5 + 2] Cycloaddition: Access to Tricyclo[6.3.1.0]dodecane.

An iron-catalyzed perezone-type [5 + 2] cycloaddition toward tricyclo[6.3.1.