Arsenite antiporters ACR3/3;1 are critical for Se-enhanced plant growth and As accumulation in As-hyperaccumulators Pteris multifida and Pteris cretica.

Selenium (Se) enhances plant growth and As accumulation in As-hyperaccumulator Pteris vittata, but its effects on As-hyperaccumulators Pteris multifida and Pteris cretica are unknown. Here, they were exposed to 50 μM arsenate (As) plus 1.25 (Se) or 2.

Isobavachalcone inhibits α-synuclein fibrillogenesis and its Parkinson's disease variants, disassembles the mature fibrils and alleviates cellular toxicity.

The accumulation of α-Synuclein (α-Syn) aggregates into neuronal inclusions is a hallmark of Parkinson's disease (PD), making α-Syn a key target for PD therapeutics. Here, we demonstrate that the chalcone compound isobavachalcone (IBC) inhibits α-Syn aggregation and remodels mature fibrils into fragmented filaments. IBC exhibits dose-dependent inhibition of wild-type α-Syn and PD-linked mutants (E46K, H50Q, G51D and A53T) while effectively abrogating seeded fibrillization.

Biogeochemical Pathways of Phytate-P Utilization in Soil: Plant and Microbial Strategies.

Phytate (salts of -inositol-1,2,3,4,5,6-hexakisphosphate) constitutes a large portion of the organic phosphorus in most soils, but its strong interactions with soil minerals and organic matter limit its availability to plants. Phytate can be used by plants only after it is desorbed from the soil matrix, with the inorganic P being released by phytases via cleavage of its phosphomonoester bonds. While plant phytases function primarily in its internal phytate remobilization, the role of microbial phytases in facilitating phytate-P utilization by plants remains poorly understood.

Performance evaluation of four antibiotics using the BD Phoenix™ NMIC-413 antimicrobial susceptibility testing panel for carbapenem-resistant and carbapenem-resistant .

Background: The spread of antimicrobial resistance (AMR) poses significant threats to human health. In 2024, the World Health Organization (WHO) classified carbapenem-resistant (CRE) as a critical-priority pathogen and carbapenem-resistant (CRPA) as a high-priority pathogen. This study aimed to evaluate the performance of meropenem (MEM), imipenem (IPM), cefepime (FEP), and cefoperazone/sulbactam (SCF) using the BD Phoenix™ NMIC-413 antimicrobial susceptibility testing (AST) panel (NMIC-413 panel) for CRE and CRPA at Nanfang Hospital, China.

Inhibition of Kv1.3 channel restrains macrophage M2 polarization and ameliorates renal fibrosis via regulating STAT6 phosphorylation.

Macrophages play crucial roles in regulating both homeostatic and inflammatory responses, with classical activated (M1) and alternatively activated (M2) subsets defined by the surrounding micro-environment. Renal fibrosis, developed from persistent inflammation, is worsened by M2 macrophages, yet the precise mechanisms underlying macrophage M2 polarization remain unclear. In this study, we investigated the role of Kv1.

Effects of social media use on employment anxiety among Chinese youth: the roles of upward social comparison, online social support and self-esteem.

Based on the continuous development of new media and mobile communication technologies, social media has significantly influenced people's daily thoughts and behaviors. For young people, social media is a platform for social interaction, and studies have found that social media helps Chinese graduates find employment. This study examined how social media use and its related factors affect employment anxiety.

Arsenite Antiporter Driven by Its Native Promoter Increases Leaf Arsenic Accumulation in Tobacco.

is the first-reported arsenic (As) hyperaccumulator, which has been applied to phytoremediation of As-contaminated soil. PvACR3, a key arsenite (AsIII) antiporter, plays an important role in As hyperaccumulation in . However, its functions in plants are not fully understood.

Novel phosphatase PvPAP1 from the As-hyperaccumulator Pteris vittata promotes organic P utilization and plant growth: Extracellular exudation and phytate hydrolysis.

Organic phosphorus (P) is a large component of soil P, but it is often unavailable for plant uptake. Purple acid phosphatases (PAP) can hydrolyze a wide range of P, playing an important role in P utilization by plants. In this study, we investigated a novel secretary PvPAP1 from the As-hyperaccumulator Pteris vittata, which can effectively utilize exogenous P, including adenosine triphosphate (ATP) and phytate.

Arsenic-enhanced plant growth in As-hyperaccumulator Pteris vittata: Metabolomic investigations and molecular mechanisms.

The first-known As-hyperaccumulator Pteris vittata is efficient in As uptake and translocation, which can be used for phytoremediation of As-contaminated soils. However, the underlying mechanisms of As-enhanced plant growth are unknown. We used untargeted metabolomics to investigate the potential metabolites and associated metabolic pathways regulating As-enhanced plant growth in P.

Deep Learning-Based Reconstruction Improves the Image Quality of Low-Dose CT Colonography.

Rationale And Objectives: To evaluate the image quality of low-dose CT colonography (CTC) using deep learning-based reconstruction (DLR) compared to iterative reconstruction (IR).

Materials And Methods: Adults included in the study were divided into four groups according to body mass index (BMI). Routine-dose (RD: 120 kVp) CTC images were reconstructed with IR (RD-IR); low-dose (LD: 100kVp) images were reconstructed with IR (LD-IR) and DLR (LD-DLR).

Spatiotemporal distribution, source analysis and ecological risk assessment of polychlorinated biphenyls (PCBs) in the Bohai Bay, China.

As a class of persistent organic pollutants (POPs), the spatial and temporal distribution of polychlorinated biphenyls (PCBs) in seawater is important for environmental assessment. Surface water samples were collected from 35 stations during summer and 36 stations during autumn of 2020 in the Bohai Bay. The concentration, composition, distribution and sources of PCBs were analyzed to assess the ecological impact of PCBs.

Distribution and association of antimicrobial resistance and virulence characteristics in spp. isolates from captive Asian elephants in China.

spp., as an opportunistic pathogen, are widely distributed in the environment and the gastrointestinal tracts of both humans and animals. Captive Asian elephants, popular animals at tourist attractions, have frequent contact with humans.

A novel TWIK2 channel inhibitor binds at the bottom of the selectivity filter and protects against LPS-induced experimental endotoxemia in vivo.

TWIK2 channel plays a critical role in NLRP3 inflammasome activation and mice deficient in TWIK2 channel are protected from sepsis and inflammatory lung injury. However, inhibitors of TWIK2 channel are currently in an early stage of development, and the molecular determinants underlying the chemical modulation of TWIK2 channel remain unexplored. In this study, we identified NPBA and the synthesized derivative NPBA-4 potently and selectively inhibited TWIK2 channel by using whole-cell patch clamp techniques.

High-sensitivity synchronous angio-lymphography based on a speckle spectrum contrast OCT.

To achieve accurate selection and synchronous imaging of blood vessels and lymph, a speckle spectrum contrast method (SSC) based on spectral-domain optical coherence tomography (SD-OCT) is proposed in this Letter. In this method, the time-lapse optical coherence tomography (OCT) intensity signal is transformed to the Fourier frequency domain. By analyzing the frequency spectrum of the time-lapse OCT intensity signal, a parameter called SSC signal, which represents the ratio of different intervals of the high frequency to the low frequency, is utilized to extract and contrast different types of the vessels in the biological tissues.

Arsenic in the hyperaccumulator Pteris vittata: A review of benefits, toxicity, and metabolism.

Arsenic (As) is a toxic metalloid, elevated levels of which in soils are becoming a major global environmental issue that poses potential health risks to humans. Pteris vittata, the first known As hyperaccumulator, has been successfully used to remediate As-polluted soils. Understanding why and how P.

Metabolomic Analysis of Microcystis aeruginosa After Exposure to the Algicide L-Lysine.

The widespread occurrence of cyanobacteria blooms damages the water ecosystem and threatens the safety of potable water and human health. Exogenous L-lysine significantly inhibits the growth of a dominant cyanobacteria Microcystis aeruginosa in freshwater. However, the molecular mechanism of how lysine inhibits the growth of M.

Seasonal variation and ecological risk assessment of Pharmaceuticals and Personal Care Products (PPCPs) in a typical semi-enclosed bay - The Bohai Bay in northern China.

The Bohai Bay as a typical semi-enclosed bay in northern China with poor water exchange capacity and significant coastal urbanization, is greatly influenced by land-based inputs and human activities. As a class of pseudo-persistent organic pollutants, the spatial and temporal distribution of Pharmaceuticals and Personal Care Products (PPCPs) is particularly important to the ecological environment, and it will be imperfect to assess the ecological risk of PPCPs for the lack of systematic investigation of their distribution in different season. 14 typical PPCPs were selected to analyze the spatial and temporal distribution in the Bohai Bay by combining online solid-phase extraction (SPE) and HPLC-MS/MS techniques in this study, and their ecological risks to aquatic organisms were assessed by risk quotients (RQs) and concentration addition (CA) model.

Toxic effects of pristine and aged polystyrene and their leachate on marine microalgae Skeletonema costatum.

The acute toxic effects of pristine and aged polystyrene (P-PS and A-PS) and their leaching solutions (L-PS) on microalgae Skeletonema costatum were investigated by measuring algal density and growth inhibition rate (IR), chlorophyll concentration and photosynthetic efficiency (Fv/Fm) over 96 h. Total protein (TP), superoxide dismutase (SOD), catalase (CAT) and malondialdehyde (MDA) were measured to analyze the oxidative damage to microalgae by microplastics and their leachates. Hydrodynamic diameter of microplastics in seawater, FITR and SEM images were used to study the changes of polystyrene during aging.

Biodiversity assessment in the near-shore waters of Tianjin city, China based on the Pressure-State-Response (PSR) method.

Parameters regarding water quality, biology, marine disasters, and anthropogenic factors were collected and analyzed from 2020 to 2021 to evaluate the marine biodiversity of Tianjin's near-shore sea area accurately. A total of 29 indicators obtained from the surveys were selected to form the biodiversity evaluation system. The Pressure-State-Response (PSR) framework model combined with the ecological environment characteristics was used as the assessment method.

Visceral fat might impact left ventricular remodeling through changes in arterial stiffness in type 2 diabetes: A cross-sectional study.

Aims: Visceral fat (VF) influences left ventricular (LV) structure and diastolic function in type 2 diabetes (T2DM). However, there are limited data on the association among them based on different BMI levels as well as accounting for arterial stiffness. This study investigated the association of fat distribution, arterial stiffness, left ventricular (LV) structure and diastolic function in T2DM patients.

[3 + 2] . [4 + 1] Annulation: revisiting mechanism studies on the phosphine-catalysed domino sequence of alkynoates and activated methylenes.

The mechanism of the phosphine-catalysed domino sequence of alkynoates and activated methylenes has been computationally studied. The computational results revealed that the [3 + 2] annulation sequence could be ruled out, due to a difficult Knoevenagel condensation of aromatic aldehydes and active methylenes. The reaction proceeds through a [4 + 1] annulation pathway, which involves a phosphine-catalysed MBH-type reaction followed by a [1,5]-proton shift and dehydration to afford vinyl phosphonium intermediates as four-carbon synthons in the annulation reaction.

Spatiotemporal distribution, ecological risk assessment and source analysis of legacy and emerging Per- and Polyfluoroalkyl Substances in the Bohai Bay, China.

The Bohai Sea is one of the most polluted hotspots by per- and Polyfluoroalkyl substances (PFASs) in the world and studies on the vertical distribution of PFASs at different water layers and phase partitioning between water and suspended particulate matter (SPM) were still limited. 23 legacy and emerging PFASs were investigated in seawater and SPM throughout the Bay in this study. The average concentrations of ∑PFASs in seawater were 48.