Fish-Derived Clostridium butyricum LZK-08: Genomic Insights and Probiotic Evaluation in Largemouth Bass.

Clostridium butyricum has gained attention as a probiotic in aquaculture due to its ability to improve growth, gut health, and immune function. However, most strains currently used are derived from non-aquatic sources, which may limit their colonization and efficacy in fish. In this study, a novel strain, C.

Impact of a MOOC-Based Blended Learning Model on Performance, Satisfaction, and Perceptions in a Histology Course for Chinese Medical Students.

Background: While massive open online course (MOOC)-based blended learning has gained popularity across disciplines, its adoption in medical histology education is underexplored, and its educational benefits for undergraduate medical students have yet to be clearly established.

Objective: This study examined the relative effectiveness of traditional and blended learning methods in terms of academic performance, student perceptions, and learning satisfaction among first-year medical students in China enrolled in a histology course.

Methods: In this study, two groups with similar age, gender, and academic background were analyzed.

Genetic diversity analysis of IVM resistant Haemonchus contortus in sheep in inner Mongolia of China.

Haemonchus contortus is widely distributed in the world, which is seriously harmful to small ruminants and has serious resistance to common anthelmintics. Over time, the prevalence and intensity of host infection with this parasite have gradually increased, so it is necessary to study the phylogenetic and genetic changes of single anthelmintic resistant strains. This study is the first to analyze the genetic diversity and population structure about IVM-resistant H.

Integrating Generative Pretrained Transformer and Genetic Algorithms for Efficient and Diverse Molecular Generation.

In computer-aided drug design, molecular generation models play a crucial role in accelerating the drug development process. Current models mainly fall into two categories: deep learning models with high performance but poor interpretability and heuristic algorithms with better interpretability but limited performance. In this study, we introduce an innovative molecular generation model, the compound construction model (CCMol), which integrates the powerful generative capabilities of the generative pretrained transformer (GPT) and the efficient optimization mechanisms of genetic algorithms (GA) to achieve effective and innovative molecular structures.

Identification of hub gene associated with colorectal cancer: Integrating Mendelian randomization, transcriptome analysis and experimental verification.

Background: Colorectal cancer (CRC) is a prevalent malignancy with significant mortality rates globally. Understanding the genetic and molecular mechanisms underlying CRC development is crucial for improving therapeutic strategies.

Method: In this study, we utilized cis-eQTL summary data to identify genes potentially causally associated with CRC.

M6PR upregulation by berberine attenuates hepatic senescence via sorting STING into endosome for degradation.

Background: Cellular senescence is associated with various hepatic diseases. Stimulator of interferon genes (STING) signaling has been identified as a significant driver of cellular senescence and hepatic stellate cells (HSCs) activation. The blockade of STING signaling serves as a potential strategy to halt senescence.

Linarin alleviates high-fat diet-induced NAFLD via modulating the PI3K/Akt/mTOR pathway, autophagy, and gut microbiota.

Linarin (Lin) is a flavonoid compound widely found in traditional herbal medicines and is recognized for its diverse biological properties, including anti-inflammatory, analgesic, antioxidant, hepatoprotective, and anti-apoptotic effects. Non-alcoholic fatty liver disease (NAFLD) is closely associated with autophagy and inflammation processes. However, the interaction between Lin and NAFLD remains underexplored.

ZNF207-driven PRDX1 lactylation and NRF2 activation in regorafenib resistance and ferroptosis evasion.

Regorafenib (RGF) is a critical second-line therapy for advanced hepatocellular carcinoma (HCC) following disease progression on sorafenib; however, the rapid onset of RGF resistance poses a significant barrier to enhancing patient outcomes. In this study, CRISPR/Cas9 screening in RGF-treated HCC cells identified Zinc Finger Protein 207 (ZNF207) as a primary driver of resistance. Further analysis revealed that ZNF207 promotes resistance by inducing antioxidant responses that inhibit ferroptosis, a form of iron-dependent cell death.

Factors affecting quality of life in patients undergoing continuous ambulatory peritoneal dialysis: A retrospective analysis.

This study aims to evaluate the quality of life (QoL) in patients undergoing continuous ambulatory peritoneal dialysis (CAPD) and to identify key clinical and nutritional factors associated with poor QoL under routine follow-up management. This retrospective study included 164 clinically stable CAPD patients who had been undergoing dialysis for >3 months at a single center. Patients were assessed using the Subjective Global Assessment tool and classified into good or poor QoL groups.

Prognostic value of the triglyceride-glucose index for continuous ambulatory peritoneal dialysis patients.

Objective: We are committed to demonstrating that the initial triglyceride-glucose (TyG) index possesses prognostic importance for patients undergoing continuous ambulatory peritoneal dialysis (CAPD).

Methods: 354 patients who utilized continuous CAPD at Wuhan No.1 Hospital were recruited.

Integration bile acid metabolomics and gut microbiome to study the anti-liver fibrosis effects of total alkaloids of Corydalis saxicola Bunting.

Background: Bile acids and gut microbiota participate in the pathogenesis of liver fibrosis (LF). The total alkaloids of Corydalis saxicola Bunting (TACS) is a traditional Chinese medicine extract that has been used to treat LF, but the underlying mechanisms are not clear. This study performed integrated metabolomics and gut microbiome analysis to study the anti-LF mechanism of TACS using a rat model.

Drug repurposing: a promising drug discovery strategy for the treatment of emerging epidemic infectious disease.

Epidemic refers to a disease when the occurrence level exceeds expected standards (generating an epidemic phenomenon), in which infectious diseases pose a major challenge to global health. The concept of epidemiology arises with infectious diseases, which can infect many people within a certain period and lead to a pandemic. Especially in the face of sudden epidemics, time is of the essence.

NIR-II Imaging Guided Accurate Identification of Paraspinal Muscle Degeneration Through Targeting the Lysosomal Membrane Protein Sidt2.

Background: This study introduces a novel imaging approach for early detection of degenerative paraspinal muscle disorders, which are a key contributor to lower back pain and lumbar-related diseases. The core concept involves the use of a lanthanide-doped nanoprobe with a core@shell structure (NaYbF₄:x%Er@NaYF₄:x%Yb@NaYF₄), designed to function as a second near-infrared (NIR-II) fluorescent probe. This probe demonstrates significant advantages such as deep tissue penetration, high spatial and temporal resolution, and exceptional stability, enabling in vivo monitoring of muscle degeneration.

Regulation of heart regeneration by LSD1 through suppressing CEND1.

Improving heart regeneration through reactivating cardiomyocyte proliferation holds a great potential for repairing diseased hearts. We recently reported that LSD1-dependent epigenetic repression of Cend1 transcription is prerequisite for cardiomyocyte proliferation and mouse heart development. This study interrogates the potential role of this LSD1-CEND1 axis in heart regeneration and repair.

Short-Term DMOG treatment rejuvenates senescent mesenchymal stem cells by enhancing mitochondrial function and mitophagy through the HIF-1α/BNIP3 pathway.

Background: Mesenchymal stem cells (MSCs) have potential for treating degenerative and immune diseases, but their clinical efficacy is limited by senescence, characterized by mitochondrial dysfunction, impaired mitophagy, and metabolic imbalance. The goal of this study was to investigate the effects of dimethyloxalylglycine (DMOG), a hypoxia-mimetic agent that stabilizes hypoxia-inducible factor 1 alpha (HIF-1α), on rejuvenating senescent MSCs by enhancing mitochondrial function, mitophagy, and metabolic reprogramming.

Methods: Two models of MSC senescence were established: oxidative stress-induced senescence using hydrogen peroxide and replicative senescence through serial passaging.

Inhibitory effect of Endostar on HIF-1 with upregulation of MHC-I in lung cancer cells.

Endostar is a human recombinant endostatin which is an attractive anti-angiogenesis protein. Because inefficient antigen presenting MHC class I expression (which can be downregulated by HIF-1) is an important strategy for cancer immune evasion, besides its anti-angiogenesis effect, it remains unclear whether Endostar has an inhibitory effect on HIF-1 expression by upregulating MHC class I expression in cancer cells to facilitate immunotherapies, including PD-1/PD-L1 inhibitors. In this study, A549 and NCI-H1299 lung cancer cells were treated with Endostar (6.

Effect of the combined addition of hyaluronic acid and gellan gum on the physicochemical and digestive properties of tofu.

The aim of this study was to increase the quality of glucono-δ-lactone (GDL)-coagulated tofu by incorporating microbial polysaccharides. The effects of different concentrations (0.1 %, 0.

Artificial Intelligence-Guided Cancer Engineering for Tumor Normalization Executed by Tumor Lysosomal-Triggered Supramolecular Chiral Peptide.

Cancer engineering for tumor normalization offers a promising therapeutic strategy to reverse malignant cells and their supportive tumor microenvironment into a more benign state. Herein, an artificial intelligence (AI) approach was developed using mRNA data from patients with lung adenocarcinoma to facilitate the identification of aberrant signaling pathways, specifically focusing on PD-L1, Wnt, and macropinocytosis. Targeting these characteristics, we have developed a supramolecular construct called cancer corrector (CCtor) with the aim of harnessing the enhanced macropinocytosis observed in cancer cells.

Network pharmacology combined with transcriptomics reveals that Ganoderma lucidum spore and Sanghuangporus vaninii compound extract exerts anti-colorectal cancer effects via CYP24A1-mediated VDR pathway and TERT-mediated Wnt signaling pathway.

Ethnopharmacological Relevance: As traditional medicinal fungi, Ganoderma lucidum and Sanghuangporus vaninii are widely used in the treatment of tumor related diseases and cancer adjuvant therapy with potent anticancer effects. However, the anticancer effect and mechanism of action of their compound extract remain unclear.

Aim Of The Study: To investigate the anticancer effect of Ganoderma lucidum and Sanghuangporus vaninii compound extract and explore the underlying mechanism.

Remodeling of Effector and Regulatory T Cells by Capture and Utilization of miRNAs Using Nanocomposite Hydrogel for Tumor-Specific Photothermal Immunotherapy.

In immunotherapy for malignant tumors, the dysregulation of the balance between effector T cells and regulatory T cells (Tregs) and the uncertain efficacy due to individual differences have been considered as two critical challenges. In this study, we engineered an injectable nanocomposite hydrogel system (SNAs@M-Gel) capable of suppressing Treg proliferation and blocking PD-1/PD-L1-mediated immune evasion effectively, achieved through the stimulus-responsive modulation of multiple tumor-associated microRNAs. Simultaneously, this system enables microRNA-dependent photothermal immunotherapy, facilitating a highly efficient and personalized approach to tumor treatment.

Engineered gold nanoparticles for accurate and full-scale tumor treatment via pH-dependent sequential charge-reversal and copper triggered photothermal-chemodynamic-immunotherapy.

Current anti-tumor strategies majorly rely on the targeted delivery of functional nanomedicines to tumor region, neglecting the importance of effective infiltration of these nanomedicines in whole tumor tissue. This process normally causes the quick endocytosis by the tumor cells at surface layer of tumor tissue, resulting in the restriction of the penetration of these nanomedicines and limited therapeutic region, which would not be able to treat the entire tumor tissue. Herein, we prepared a series of engineered gold nanoparticles (Au-MBP NPs) with step-wise charge reversal in different acid environments that could entirely infiltrate into the whole tumor tissue and then perform tumor-specific photothermal-chemodynamic-immunotherapy to achieve the complete and accurate tumor treatment.

Using explainable machine learning to predict the irritation and corrosivity of chemicals on eyes and skin.

Contact with specific chemicals often results in corrosive and irritative responses in the eyes and skin, playing a pivotal role in assessing the potential hazards of personal care products, cosmetics, and industrial chemicals to human health. While traditional animal testing can provide valuable information, its high costs, ethical controversies, and significant demand for animals limit its extensive use, particularly during preliminary screening stages. To address these issues, we adopted a computational modeling approach, integrating 3316 experimental data points on eye irritation and 3080 data points on skin irritation, to develop various machine learning and deep learning models.

Diverse Physiological Roles of Kynurenine Pathway Metabolites: Updated Implications for Health and Disease.

Tryptophan is an essential amino acid critical for human health. It plays a pivotal role in numerous physiological and biochemical processes through its metabolism. The kynurenine (KYN) pathway serves as the principal metabolic route for tryptophan, producing bioactive metabolites, including KYN, quinolinic acid, and 3-hydroxykynurenine.

Identification of RCC2 as a Risk Gene Associated With Basal Cell Carcinoma and Experimental Validation.

Basal cell carcinoma (BCC) is a common type of skin cancer that is increasing in prevalence worldwide. Previous genome-wide association studies (GWAS) have identified certain genetic loci associated with BCC. However, many potential disease-causing genes of BCC remain to be discovered.

Amentoflavone protects against cisplatin-induced acute kidney injury by modulating Nrf2-mediated oxidative stress and ferroptosis and partially by activating Nrf2-dependent PANoptosis.

Background: Cisplatin is a widely used drug for the treatment of solid organ cancer, but its renal toxicity cannot be ignored. Amentoflavone (AME), a natural flavonoid compound, has remarkable pharmacological effects, including anti-inflammatory and antioxidative effects. The effect and mechanism of AME on cisplatin-induced acute kidney injury (CI-AKI) remain unclear.