CXCL12-targeting siRNA nanoparticles alleviate immunosuppression and inhibit tumor progression in esophageal squamous cell carcinoma.

Esophageal squamous cell carcinoma (ESCC) is associated with a highly immunosuppressive tumor microenvironment (TME), driven in part by cancer-associated fibroblasts (CAFs) that promote immune evasion through the secretion of CXCL12. CXCL12 interacts with the CXCR4 receptor on immune cells, disrupting CD8 T cell migration and anti-tumor function. To address this, we developed an innovative siRNA-based therapeutic approach targeting CXCL12 in CAFs.

Effectiveness of pay it forward intervention compared to free and user-paid vaccinations on seasonal influenza vaccination among older adults across seven cities in China: study protocol of a three-arm cluster randomized controlled trial.

Background: Seasonal influenza poses an enormous burden worldwide, with older adults facing preventable morbidity and mortality. However, seasonal influenza vaccination coverage is poor among older populations in China. Pay-it-forward (giving a person a free vaccine and an opportunity to donate to support others) and financial incentives (e.

Discovery of a Potent, Selective, and Multiple His435 Mutation-Sensitive Thyroid Hormone Receptor β Agonist.

Beyond selectivity concerns for thyroid hormone receptor β (THR-β) agonists, intolerance to the His435 mutation remains a challenge. Following our previous study, we performed detailed modifications on the 7-position of isoquinoline, specifically targeting the hydrophobic region of the THR-β ligand-binding pocket (LBP). This led to the identification of compound , which showed potent THR-β agonistic activity (EC: 3.

Responses of soil fertility indicators and fungi community diversity to fertilization strategies in legume-grass mixtures.

Introduction: Alfalfa-grass binary mixtures outperformed monocultures in nutrient use, soil nutrient content, and biomass yield. Nonetheless, the impact of fertilization strategies on fungal community composition and ecological functions in legume-Grass mixtures remains under-researched. This study aimed to explore the effects of different fertilization strategies on soil fungal community distribution and soil environmental characteristics.

Subcutaneous adipose tissue [F]FDG uptake and CT-derived body composition variables for predicting survival outcomes in patients with locally advanced gastric cancer.

Purpose: This study aimed to investigate the impact of glucose metabolism in subcutaneous adipose tissue (SAT) and skeletal muscle (SM) variables on overall survival (OS) in patients with locally advanced gastric cancer.

Methods: A retrospective study was conducted on 110 patients with advanced gastric cancer who underwent baseline [F]FDG PET/CT. Demographic, clinical, and survival data were collected.

Brief verbal intervention to address inappropriate prescriptions of chinese patent medicines among western practitioners in primary health care (BRAVERY): a study protocol for an unannounced standardized patient experiment with a factorial design randomized controlled trial in China.

Introduction: Chinese patent medicines, produced using advanced pharmaceutical techniques and available in various forms, including powders, granules, tablets, pills, and capsules, finds extensive utilization among Western medicine practitioners in primary healthcare (WMP-PHC). However, the inappropriate overprescribing of these medicines has led to significant resource waste and raised considerable concerns. Therefore, we aim to address related knowledge gaps by employing unannounced standardized patients (USPs) as a method, for both measurement and intervention research.

Regulatory T Cell Infiltration-Driven Single-Cell Transcriptomic Analysis Identifies SAP18 as a Prognostic Marker for Esophageal Squamous Cell Carcinoma.

Background: Advanced esophageal squamous cell carcinoma (ESCC) is characterized by molecular heterogeneity and distinct patterns of immune cell infiltration. Regulatory T cells (Tregs), in particular, play a critical role in shaping an immunosuppressive tumor microenvironment (TME), which is associated with poor clinical outcomes.

Methods: We developed a prognostic model by integrating GEO-derived bulk RNA sequencing data and single-cell transcriptome.

Preclinical Evaluation and First-in-Human Study of [Ga]Ga-αvβ6-2: A Novel Dimeric Integrin αvβ6-Targeted PET Probe for Pancreatic Cancer Imaging.

Integrin αvβ6 is highly expressed in pancreatic cancer, making it an ideal target for molecular imaging diagnosis. Multimerization is considered an effective strategy to increase the accumulation of molecular probes in tumors. Here, we synthesized monomeric and dimeric αvβ6-targeting molecular probes, labeled with Ga, and designated them [Ga]Ga-αvβ6-1 and [Ga]Ga-αvβ6-2, respectively.

Identification and functional analysis of proteins in Anabarilius grahami at different growth stages based on 4D-DIA quantitative proteomics technology.

This study compared the proteome of Anabarilius grahami at three growth stages with Carassius auratus at a single growth stage to identify and analyze protein functions in A. grahami using quantitative proteomics technology. A total of 1821 proteins were identified, among which six differentially abundant proteins (DAPs) were found as biomarkers for discriminating C.

Image quality, diagnostic performance of reduced-dose abdominal CT with artificial intelligence model-based iterative reconstruction for colorectal liver metastasis: a prospective cohort study.

Background: The optimization of regularization strategies in computed tomography (CT) iterative reconstruction may allow for a reduced dose (RD) without compromising image quality, thus the diagnostic ability of RD imaging must be considered, especially for low-contrast lesions. In this study, we evaluated the image quality and diagnostic performance of 50% RD CT for low-contrast colorectal liver metastasis (CRLM) with artificial intelligence model-based iterative reconstruction (AIIR) and standard-dose (SD) CT with hybrid iterative reconstruction (HIR).

Methods: In this prospective study, consecutive participants with pathologically proven colorectal cancer and suspected liver metastases who underwent portal venous phase CT scans both at SD and RD between June and November 2022 were included.

Protein-Interference-Free and Kidney-Targeting NIR Fluorophores for Accurate in Vivo Imaging of HS during Kidney Ferroptosis.

High-fidelity kidney function imaging is important for assessing the nephrotoxicity of drugs and diagnosing renal diseases. However, the current challenges in achieving accurate kidney imaging include unspecific signal enhancement due to albumin binding and relatively low distribution of imaging agents in kidneys. Here, for the first time, a side-chain engineering strategy that incorporates hydrophilic six-membered heterocycles into aza-hemicyanine for generating high-performance kidney imaging agents with protein-interference-free and kidney-targeting features is proposed.

BcSRC2 interacts with BcAPX4 to increase ascorbic acid content for responding ABA signaling and drought stress in pak choi.

As a reducing substance, ascorbic acid functioned well in abiotic and biotic stress. However, the regulatory mechanism of drought resistance is rarely known in pak choi. Here we found a gene containing a C2 domain that responds to ABA signal and drought regulation in pak choi.

Ga-DOTA-FAPI-04 and F-FDG PET/CT: a head-to-head comparison for peritoneal carcinomatosis diagnostic accuracy.

Purpose: The study aimed to compare the diagnostic accuracy of Ga-DOTA-FAPI-04 (Ga-FAPI) and F-FDG PET/CT for peritoneal carcinomatosis (PC) in patients with various types of cancer.

Methods: The study enrolled 113 patients with suspected peritoneal malignancy, each of whom underwent Ga-FAPI and F-FDG PET/CT scans. Lesions in all patients were confirmed through pathology or radiological follow-up.

Associations between self-reported sleep duration and incident cardiovascular diseases in a nationwide prospective cohort study of Chinese middle-aged and older adults.

Purpose: This study explores the correlation between sleep duration and cardiovascular disease (CVD) among middle-aged and older adults in China. Furthermore, we aim to investigate the association between sleep duration and incident CVD in this population, while assessing potential variations across different age and gender subgroups.

Methods: Utilizing data from the nationwide prospective survey of the China Health and Retirement Longitudinal Study (CHARLS) conducted in 2011, 2013, 2015, and 2018, involving 17,596 participants aged 45 years and above, we employed Cox proportional hazards regression models.

TRIM22 mechanism promoting KAT2A ubiquitination degradation to regulate ferroptosis in hepatocellular carcinoma cell invasion and metastasis.

Objective: Hepatocellular carcinoma (HCC) is a highly fatal cancer. This study aims to investigate the underlying mechanism of tripartite motif-containing 22 (TRIM22) in HCC cell invasion and metastasis through the K (lysine) acetyltransferase 2A (KAT2A)/glutathione peroxidase 4 (GPX4) axis.

Methods: Human HCC cells BEL7405 were cultured and treated with MG-132, Ferrostain-1, pcDNA3.

Accuracy of Ultrasound Measurements of Muscle Thickness in Identifying Older Patients With Sarcopenia and Its Impact on Frailty: A Systematic Review and Meta-Analysis.

Objectives: The aim of this systematic review was to assess the diagnostic test accuracy of muscle ultrasound for identifying older patients with sarcopenia and to investigate its association with frailty.

Design: Systematic review and meta-analysis of observational studies. Comprehensive searches were conducted in PubMed, MEDLINE, Cochrane Library, Scopus, and Embase through October 2024.

BcVQ11A-BcWRKY23-BcWRKY25 Module Is Involved in Thermotolerance by Regulating Phenylalanine Ammonia-Lyase Activity in Non-Heading Chinese Cabbage.

High temperature can significantly affect the quality and yield of plants. However, there has been limited research investigating the thermotolerance of non-heading Chinese cabbage (NHCC). This study, identified BcWRKY23 through transcriptome analysis in NHCC with varying levels of thermotolerance.

Putative Identification of 47 Compounds from Jieyu Anshen Granule and Proposal of Pharmacopeia Quality-Assessment Strategy Using TCM-Specific Library with UHPLC-Q-Exactive-Orbitrap-MS.

Jieyu Anshen Granule is a traditional Chinese medicine prescription used for depression and primarily comprises five herbal medicines: Zhizi, Chaihu, Zhigancao, Danggui, and Yuanzhi. This study established a traditional Chinese herbal medicine-specific library using emerging ultra-high-performance liquid chromatography-quadrupole-orbitrap-tandem mass spectrometry analysis. Through library comparison, the study has fulfilled isomers distinction.

A head-to-head comparison of [Ga]Ga-DOTA-FGFR1 and [F]FDG PET/CT in the diagnosis of lung cancer.

Purpose: This study aimed to compare the diagnostic value of [Ga]Ga-DOTA-FGFR1 and [F]FDG PET/CT in the evaluation of lung cancer patients.

Methods: A prospective study was conducted between March 2023 and July 2023. Patients with high clinical suspicion of lung cancer were recruited.

Efficient genetic transformation and gene editing of Chinese cabbage using Agrobacterium rhizogenes.

A method using -mediated callus production and plant regeneration enables efficient genetic transformation and gene editing in Chinese cabbage.

Chinese American Pain Experience Project (CAPE): Perceptions, Expectations, and Attitudes on Pain Management among Chinese American Postoperative Patients.

Introduction: Chinese Americans are one of the fastest growing racial and ethnic groups and represent the largest subgroup of the Asian American population in the US and in New York City (NYC) where they number 573,528 in 2021. Despite their numbers, current pain perceptions, expectations, and attitudes of Chinese Americans remains poorly understood, especially as related to postoperative pain.

Objective: A better understanding of pain experience among Chinese American patients is needed to inform strategies on improving pain management satisfaction.

Baseline F-FDG PET Radiomics Predicting Therapeutic Efficacy of Diffuse Large B-Cell Lymphoma after R-CHOP (-Like) Therapy.

This study aimed to predict therapeutic efficacy among diffuse large B-cell lymphoma (DLBCL) after R-CHOP (-like) therapy using baseline F-fluorodeoxyglucose positron emission tomography (F-FDG PET) radiomics. A total of 239 patients with DLBCL were enrolled in this study, with 82 patients having refractory/relapsed disease. The radiomics signatures were developed using a stacking ensemble approach.