Genome-Wide Identification of the Dirigent Gene Family and Expression Pattern Analysis Under Drought and Salt Stresses of (L.).

The () gene family is pivotal for lignin polymerization and stress adaptation in plants, yet its systematic characterization in (), a critical bioenergy crop, remains underexplored. Leveraging the genome database, we conducted a genome-wide identification, phylogenetic classification, and expression profiling of the gene family. Evolutionary dynamics, gene structure variations, promoter cis-regulatory elements, and spatiotemporal transcriptome patterns were analyzed using bioinformatics and experimental validation (RT-qPCR).

Pseudomonas bambusae sp.nov., an Aniline Blue-Decolorizing Bacterium Isolated from Decaying Round Bamboo Culms.

A novel bacterial strain, designated RC10, was isolated from decaying round bamboo culms. Phylogenetic and sequence similarity analysis of 16S rRNA gene sequences indicated that strain RC10 was grouped with members of the genus Pseudomonas, with the highest similarity of 98.4% to Pseudomonas lutea DSM 17257, followed by Pseudomonas kuykendallii DSM 25107 (98.

Modified discharge capacity calculation method for piano key weirs.

Due to the numerous geometric parameters and complex hydraulic behaviors of piano key weirs (PKWs), accurately estimating their discharge capacity remains challenging without in-depth experimental studies or computational modeling. This study compares and analyzes recent discharge equations and develops new relationships between key parameters influencing the discharge capacity of PKWs, including the weir height , weir width , and effective crest length . A modified theoretical discharge equation is proposed, derived from dimensional analysis and supported by existing experimental data.

sp. nov., a novel member of the family , isolated from a saline lake.

A Gram-stain-negative, aerobic bacterium, designated W635, was isolated from saline lake water collected from Lake Ayaqqum in Xinjiang province, China. Cells were catalase and oxidase positive, rod-shaped and non-motile. Growth occurred at 20-37 °C (optimum 28-30 °C), pH 7.

Antifreezing and Temperature-Responsive Ionic Hydrogels with Applications in Encryption and Sensor Technologies.

The use of thermoresponsive hydrogels for applications such as sensors, thermal gates, smart windows, actuators, and molecular devices has increased in popularity in the past decade. However, existing thermoresponsive biobased hydrogel sensors face challenges in rapidly responding to ambient temperature changes and retaining flexibility at subzero temperatures. To overcome these limitations, a novel hydrogel composed of dextrin, glycerol, and the ionic liquid monomer tetrabutylphosphonium styrenesulfonate (PSS) was developed and utilized as a smart sensor material for the first time.

Effects of different peripheral fatigue protocol on lower limb biomechanical changes during landing and its impact on the risk of anterior cruciate ligament injury: a systematic review.

Previous research suggests that although fatigue accumulates during competitions and at season's end, anterior cruciate ligament (ACL) injuries do not significantly increase, with peripheral fatigue-induced muscle weakness potentially playing a key role. The aim of this study is to systematically review the effects of different peripheral fatigue interventions on biomechanical variables associated with ACL injury risk during landing tasks. A systematic search was conducted in five databases Web of Science, Scopus, PubMed, EBSCO, and Cochrane Library up to September 2024.

Self-Healing, Remoldable, and Conductive Starch-Based Dual Reversible Cross-Linking Hydrogels for Strain Sensors.

Polysaccharide-based hydrogels have been utilized as flexible strain sensors because of their renewability, biocompatibility, and biodegradability. However, their widespread application is hindered by the complexity of their manufacturing processes and the inevitable degradation of their mechanical properties with repeated use. The introduction of reversible bond chemistry offers the potential to impart self-healing properties to hydrogels, extending their functional lifespan.

Impact of arm position on low dose dual-source CT one-step aortic and cerebral-carotid artery angiography image quality and radiation dose.

Background: Although one-stop cerebral carotid aortic CTA is useful for diagnosing and treating type A aortic dissection, the radiation dose is increased due to the wider scanning range. The impact of varying arm positions on radiation dose and image quality is unknown when optimizing the scan protocol. This study aims to determine the best scanning protocol to minimize radiation dose while maintaining image quality, as well as how arm position impacts radiation dose and image quality of low dose one-stop cerebral carotid aortic CTA.

ADSCs attenuate Liver fibrosis via inducing HSC senescence: validation in dual-etiology models.

Background: Liver fibrosis (LF) results from various causes, which require finding conserved mechanisms to help treat related diseases. Although adipose-derived stem cells (ADSCs) transplantation can alleviate hepatic fibrosis, their mechanism remains unclear. Accordingly, we explored the efficacy and mechanisms behind ADSCs transplantation in two LF models.

Method development for the detection of anti-drug antibodies against a therapeutic peptide: assay format selection.

Aim: Monitoring immune responses to therapeutic peptides with endogenous counterparts is crucial for evaluating drug safety and efficacy. In this paper, we focused on the selection of an optimal assay format to develop a sensitive, robust, and drug-tolerant immunoassay for the detection of anti-drug antibody (ADA) against a therapeutic peptide.

Results: We assessed distinct ADA assay formats for preclinical and clinical studies, such as direct binding with labeled protein A/G, direct binding with labeled multiple species-specific antibodies for detection, bridging and affinity capture elution (ACE) formats.

Tumor-specific cathepsin B-triggered fluorescence imaging and prodrug activation.

Bioorthogonal activation chemistries have great potential in the development of novel drug treatments due to their versatility, tunability, and the ability to generate therapies with improved spatial targeting. The upregulation of Cathepsin B is highly correlated with the development of cancers, however, few fluorescent probes or prodrugs-based on Cathepsin B activity have demonstrated high tumor selectivity, since Cathepsin B is expressed in a variety of normal tissues. In this study, we report a strain-promoted azide-alkyne cycloaddition-activation strategy whereby a para-azido safety-catch linker is triggered by the tumor locating Biotin-TCO (trans-cyclooctene) conjugate, with subsequent tumor-specific Cathepsin B-triggered activation, generating a fluorescent reporter/cytotoxic drug, with high tumor selectivity.

Mesoscopic modeling the interaction of two attached-wall cavitation bubbles.

A hybrid thermal lattice Boltzmann cavitation model based on a nonorthogonal framework is developed to investigate the interaction of two attached-wall cavitation bubbles. The interaction modes are systematically analyzed, with an emphasis on how varying contact angles influence the flow and temperature distributions, as well as the evolution of wall heat flux under strong and weak interaction conditions. Bubbles formed on the hydrophobic surface display increased contact radius and greater curvature radii compared to those on the hydrophilic wall, leading to greater volumes but weaker collapse intensity.

Gut microbial-derived N-acetylmuramic acid alleviates colorectal cancer via the AKT1 pathway.

Background: Gut microbial metabolites are recognised as critical effector molecules that influence the development of colorectal cancer (CRC). Peptidoglycan fragments (PGFs) produced by microbiota play a crucial role in maintaining intestinal homeostasis, but their role in CRC remains unclear.

Objective: Here, we aimed to explore the potential contribution of PGFs in intestinal tumourigenesis.

Integrating temporal dynamics of both microbial taxonomic and functional characteristics as a tool for forensic time since deposition estimation.

Predicting the time since deposition (TsD) of body fluid stain at a crime scene is highly valuable in forensic investigation, as it can connect a DNA-identified stain donor to a crime or estimate the post-mortem interval in forensic case involving cadaver. Previous study has demonstrated the applicability of microbial taxa for TsD prediction. In this proof-of-concept study, we explored the combined use of taxonomic and functional characteristics in human saliva microbiome for TsD prediction.

Whole-Genome Sequencing and Fine Map Analysis of .

(T. Ito) S. Ito and S.

Heat Tolerance Differences Between Hu Sheep and Hu Crossbred Sheep in Microbial Community Structure and Metabolism.

Background: The frequent occurrence of extreme temperature events causes significant economic losses to the livestock industry. Therefore, delving into the differences in the physiological and molecular mechanisms of heat stress across different sheep breeds is crucial for developing effective management and breeding strategies.

Methods: This study explores the differences in heat tolerance mechanisms between Hu sheep and Xinggao sheep by comparing their growth performance under normal and heat stress conditions, as well as examining the differences in physiological, biochemical, and antioxidant indicators related to heat tolerance, serum metabolomics, and gut microbiomics in a heat stress environment.

The gut-brain axis underlying hepatic encephalopathy in liver cirrhosis.

Up to 50-70% of patients with liver cirrhosis develop hepatic encephalopathy (HE), which is closely related to gut microbiota dysbiosis, with an unclear mechanism. Here, by constructing gut-brain modules to assess bacterial neurotoxins from metagenomic datasets, we found that phenylalanine decarboxylase (PDC) genes, mainly from Ruminococcus gnavus, increased approximately tenfold in patients with cirrhosis and higher in patients with HE. Cirrhotic, not healthy, mice colonized with R.

Selection signature analysis reveals genes associated with tail phenotype in sheep.

Tail type of sheep, which may be affected by many genes with a complex mechanism, is an important economic trait concerned by both raiser and consumers. Here, we employed two sheep breeds with extreme phenotypes - Mongolian sheep (short-fat-tailed) and Bamei Mutton sheep (long-thin-tailed) to analyze the genetic differences at the genomic level and find candidate genes associated with tail phenotype. The results of population structure analysis showed that the LD decay rate of Mongolian sheep was greater than that of Bamai Mutton sheep.

Transcriptome-Based Revelation of the Effects of Sleep Deprivation on Hepatic Metabolic Rhythms in Tibetan Sheep ().

Sleep deprivation (SD) disrupts circadian rhythms; however, its effects on SD and the mechanisms involved require further investigation. Previous studies on SD were mainly conducted on rodents, such as mice, with few studies on its effects on the liver of large diurnal animals, such as sheep. In this study, we used a Tibetan sheep model for the first time to investigate the effects of SD on the liver by exposing Tibetan sheep () to 7 days of SD (6 h/day) and performed transcriptome sequencing analysis on liver samples taken at 4 h intervals over 24 h.

identification and verification of Tanshinone IIA-related prognostic genes in hepatocellular carcinoma.

Background: Currently, adequate treatment and prognostic prediction means for Hepatocellular Carcinoma (HCC) haven't entered into medical vision. Tanshinone IIA (TanIIA) is a natural product, which can be utilized as a potential treatment of HCC due to its high anti-tumor activity. However, the effect on HCC prognosis, as well as the potential targets and molecular mechanism of TanIIA still remain ambiguous.

Environmental Heat Stress Decreases Sperm Motility by Disrupting the Diurnal Rhythms of Rumen Microbes and Metabolites in Hu Rams.

Heat stress (HS) has become a common stressor, owing to the increasing frequency of extreme high-temperature weather triggered by global warming, which has seriously affected the reproductive capacity of important livestock such as sheep. However, little is known about whether HS reduces sperm motility by inducing circadian rhythm disorders in rumen microorganisms and metabolites in sheep. In this study, the year-round reproduction of two-year-old Hu rams was selected, and the samples were collected in May and July 2022 at average environmental temperatures between 18.

Effects of dietary paddy rice on the growth, serum biochemistry, intestinal development, microbiota, and metabolism of young laying ducks in a rice-duck-crayfish farming system.

This study investigated the effects of feeding paddy rice on the physiology, metabolism, and gut microbiota of ducks in a rice-duck-crayfish (RDC) system. A total of 540 ducks (20-days-old) were randomly divided into 3 groups with 3 replicates and 60 ducks per replicate. The 40-d experiment involved 3 diet treatments: a complete diet (CD), 50% paddy rice + 50% complete diet (RCD), and 100% paddy rice diet (RD).

Prediction of the potential distribution of a raspberry (Rubus idaeus) in China based on MaxEnt model.

Rubus idaeus is a pivotal cultivated species of raspberry known for its attractive color, distinct flavor, and numerous health benefits. It can be used in pharmaceutical, cosmetics, agriculture and food industries not only as fresh but also as a processed product. Nowadays due to climatic changes, genetic diversity of cultivars has decreased dramatically.

Evolution, Gene Duplication, and Expression Pattern Analysis of Gene Family in (L.).

Catharanthus roseus receptor-like kinase 1-like (CrRLK1L) plays pivotal roles in regulating plant growth and development, mediating intercellular signal transduction, and modulating responses to environmental stresses. However, a comprehensive genome-wide identification and analysis of the gene family in maize remains elusive. In this study, a total of 24 genes were identified in the maize whole genome.