A novel hydrogel-coated chest drain based on ropivacaine-glycerol-alginate hydrogel with construction and application to postoperative thoracic rehabilitation.

We aimed to develop a drug-loaded hydrogel-encapsulated chest drain to improve postoperative comfort and recovery in thoracic surgery patients. The hydrogel was modified with different ratios of glycerol and alginate, then mixed with varying concentrations of ropivacaine and fixed on a simulated chest drain tube using a mould and calcium chloride solution. The morphology, degradation, and slow-release properties of the hydrogel were assessed to identify the most suitable formulation.

Nuclear Pirin promotes HCC by acting as a key inflammation-facilitating factor.

Background: Chronic inflammation and elevated reactive oxygen species are key contributors to hepatocellular carcinoma (HCC) progression.

Objective: This study aims to investigate the role of the oxidative stress sensor protein Pirin (PIR) as a critical mediator of inflammation in HCC progression.

Design: We investigated PIR's role in HCC tumourigenesis through RNA interference, genetic knockout and pharmaceutical inhibition in HCC cell lines and various mouse models.

Differential Analysis of Anthocyanins in Red and Yellow Hawthorn () Peel Based on Ultra-High Performance Liquid Chromatography-Electrospray Ionization Tandem Mass Spectrometry.

Anthocyanins constitute the primary pigment components in hawthorn () peel, yet their specific composition and concentration profiles remain poorly characterized. This study employed ultra-performance liquid chromatography-electrospray ionization-tandem mass spectrometry (UPLC-ESI-MS/MS)-based metabolomics to systematically compare anthocyanin profiles between red-peel (CPR) and yellow-peel (CPY) hawthorn cultivars. Our analysis identified 26 anthocyanin metabolites in CPR and 24 in CPY, with cyanidin-3-O-galactoside and cyanidin-3-O-arabinoside being the predominant compounds in both.

Perioperative changes in lung re-transplantation: a single-centre observational study.

Background: Reports describing the management of lung re-transplantation (Re-LTx) are lacking. Therefore, this study evaluated the characteristics of Re-LTx to guide perioperative management.

Methods: This study retrospectively analysed the clinical data of patients who underwent primary lung transplantation (primary-LTx) and Re-LTx in the First Affiliated Hospital of Guangzhou Medical University from 2016 to 2023.

Chromosome-level de novo genome unveils the evolution of Gleditsia sinensis and thorns development.

Gleditsia sinensis Lam. (G. sinensis) as an important species within the Leguminosae family, has been utilized in Chinese medicine for centuries, and its thorns serve as a chief medicinal ingredient.

Mitochondrial Genome Assembly and Comparative Analysis of (Oleaceae).

: Lindl. & Paxton is an ornamental tree species native to North China. Research on the mitochondrial genome can elucidate the evolution and biological characteristics of and better protect this important species.

Prediction of the potential distribution of a raspberry (Rubus idaeus) in China based on MaxEnt model.

Rubus idaeus is a pivotal cultivated species of raspberry known for its attractive color, distinct flavor, and numerous health benefits. It can be used in pharmaceutical, cosmetics, agriculture and food industries not only as fresh but also as a processed product. Nowadays due to climatic changes, genetic diversity of cultivars has decreased dramatically.

The proto-oncogene tyrosine kinase c-SRC facilitates glioblastoma progression by remodeling fatty acid synthesis.

Increased fatty acid synthesis benefits glioblastoma malignancy. However, the coordinated regulation of cytosolic acetyl-CoA production, the exclusive substrate for fatty acid synthesis, remains unclear. Here, we show that proto-oncogene tyrosine kinase c-SRC is activated in glioblastoma and remodels cytosolic acetyl-CoA production for fatty acid synthesis.

Pirin Inhibits FAS-Mediated Apoptosis to Support Colorectal Cancer Survival.

Resistance to immunotherapy in colorectal cancer (CRC) is associated with obstruction of FAS (Apo-1 or CD95)-dependent apoptosis, a hallmark of cancer. Here it is demonstrated that the upregulation of pirin (PIR) protein in colon cancers promotes tumorigenesis. Knockout or inhibition of PIR dramatically increases FAS expression, FAS-dependent apoptosis and attenuates colorectal tumor formation in mice.

Chromosome-level genome assembly and population genetic analysis of a near-threatened rosewood species ( Pierre Graham ex Benth) provide insights into its evolutionary and cold stress responses.

Pierre Graham ex Benth () is a precious rosewood tree species that grows in the tropical and subtropical regions of Asia. In this study, we used PacBio long-reading sequencing technology and Hi-C assistance to sequence and assemble the reference genome of . We generated 171.

ELP3 stabilizes c-Myc to promote tumorigenesis.

ELP3, the catalytic subunit of the Elongator complex, is an acetyltransferase and associated with tumor progression. However, the detail of ELP3 oncogenic function remains largely unclear. Here, we found that ELP3 stabilizes c-Myc to promote tumorigenesis in an acetyltransferase-independent manner.

ZEB1 Transcriptionally Activates PHGDH to Facilitate Carcinogenesis and Progression of HCC.

Background & Aims: Phosphoglycerate dehydrogenase (PHGDH), the rate-limiting enzyme of the de novo serine synthesis pathway (SSP), has been implicated in the carcinogenesis and metastasis of hepatocellular carcinoma (HCC) because of its excessive expression and promotion of SSP. In previous experiments we found that SSP flux was diminished by knockdown of zinc finger E-box binding homeobox 1 (ZEB1), a stimulator of HCC metastasis, but the underlying mechanism remains largely unknown. Here, we aimed to determine how SSP flux is regulated by ZEB1 and the contribution of such regulation to carcinogenesis and progression of HCC.

The Genus as a Novel Forest Crop: A Review of Its Genetic Resources, Nutritional Components, Biosynthesis, and Biological Studies.

The genus belongs to the Lardizabalaceae family and comprises five species that are primarily distributed in East Asia. Plants of the genus comprise deciduous and semi-evergreen perennial twining vines that have been used in Chinese herbal medicine for at least 2000 years. The plants of this genus have the potential to form a novel forest crop with high nutritional and economic value because their fruit has a delicious sweet taste and rich nutrient components.

Characterization of the complete chloroplast genome sequences of six species and its comparative analysis in the subfamily of Papilionoideae (Fabaceae).

spp. are numerous and widely distributed in pantropical areas in Asia, Africa and America, and most of the species have important economic and ecological value as precious timber. In this study, we determined and characterized six complete chloroplast genomes of species (, , , , , ), which displayed the typical quadripartite structure of angiosperms.

Accumulation of Anthocyanidins Determines Leaf Color of Liquidambar Formosana as Revealed by Transcriptome Sequencing and Metabolism Analysis.

is important for its ornamental value in China; it is increasingly used for landscaping and gardening trees due to its diverse leaf colors and seasonal changes. Varieties including either a fixed leaf color, the purplish 'Fuluzifeng' (ZF), or seasonal changes in leaf color, the reddish 'Nanlinhong' (NLH) have been bred and registered as new plant varieties under the International Union for the Protection of New Plant Varieties (UPOV) system. To gain practical insights into the anthocyanin biosynthetic process, transcriptome sequencing (Illumina) was performed to clarify the metabolic pathways present in the three seasonal changes in leaf colors in NLH and in the springtime purple-red color of ZF.

Filamentous GLS1 promotes ROS-induced apoptosis upon glutamine deprivation via insufficient asparagine synthesis.

GLS1 orchestrates glutaminolysis and promotes cell proliferation when glutamine is abundant by regenerating TCA cycle intermediates and supporting redox homeostasis. CB-839, an inhibitor of GLS1, is currently under clinical investigation for a variety of cancer types. Here, we show that GLS1 facilitates apoptosis when glutamine is deprived.

Water-soluble and dispersible porous organic polymers: preparation, functions and applications.

Porous organic polymers (POPs) have attracted increasing attention and emerged as a new research area in polymer chemistry. During the past decade, the intense desirability for application in aqueous scenarios has spawned the development of a specific class of POPs, , water-soluble or dispersible porous organic polymers (WS-POPs) that can allow the implementation of porosity-based functions in aqueous media. In this Tutorial Review, aiming at providing a practical guide to this area, we will discuss recent advances in the preparation of WS-POPs through covalent/dynamic covalent, coordination and supramolecular approaches.