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Article Abstract
Pierre Graham ex Benth () is a precious rosewood tree species that grows in the tropical and subtropical regions of Asia. In this study, we used PacBio long-reading sequencing technology and Hi-C assistance to sequence and assemble the reference genome of . We generated 171.47 Gb PacBio long reads and 72.43 Gb Hi-C data and yielded an assembly of 10 pseudochromosomes with a total size of 690.99 Mb and Scaffold N50 of 65.76 Mb. The analysis of specific genes revealed that the triterpenoids represented by lupeol may play an important role in 's potential medicinal value. Using the new reference genome, we analyzed the resequencing of 19 accessions and found that and have the latest genetic relationship. Transcriptome sequencing of leaves grown under cold stress revealed that MYB transcription factor and E3 ubiquitin ligase may be playing an important role in the cold response of . Genome resources and identified genetic variation, especially those genes related to the biosynthesis of phytochemicals and cold stress response, will be helpful for the introduction, domestication, utilization, and further breeding of species.
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC10552272 | PMC |
| http://dx.doi.org/10.3389/fpls.2023.1212967 | DOI Listing |
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