236 metagenome-assembled microbial genomes from rivers along a latitudinal gradient.

Rivers are dynamic ecosystems that play a crucial role in supporting microbial diversity and sustaining a wide range of ecological functions. Here, we used metagenomic sequencing datasets of channel sediments, riparian bulk soils, and riparian rhizosphere soils to construct metagenome-assembled genomes (MAGs) from 30 river wetlands along a latitudinal gradient in China. We identified 236 MAGs with completeness ≥ 50% and contamination ≤ 10%, including 225 bacteria and 11 archaea.

A QS21+ CpG-Adjuvanted Rabies Virus G Subunit Vaccine Elicits Superior Humoral and Moderate Cellular Immunity.

Background: Rabies remains a fatal zoonotic disease caused by rabies virus (RABV), posing substantial global health challenges. Current vaccine production faces challenges in manufacturing efficiency and cost-effectiveness. The RABV glycoprotein (RABV-G) serves as the key antigen for eliciting protective immunity.

Transcriptomic dynamics and immune-associated SNP discovery in Monopterus albus infected with Chinese rice-field eel rhabdovirus.

Viral infections represent a significant challenge to the health and productivity of Chinese rice-field eel (Monopterus albus) aquaculture. In this study, a 108-h preliminary experiment identified the critical immune response window between 24 and 48 h post-infection (hpi) with Chinese rice-field eel rhabdovirus (CrERV). Based on this, kidney and spleen tissues were sampled at 24 and 48 hpi for histopathological analysis using hematoxylin and eosin staining.

Plant diversity promotes soil nitrogen retention and removal processes in wetlands.

Although nitrogen (N) cycling processes are fundamental to the functioning of wetland ecosystems, the effects and underlying mechanisms of plant diversity on soil N cycling remain insufficiently understood. We conducted a field survey (123 soil samples) and a pot experiment (528 soil samples) to examine how multidimensional plant diversity (species diversity, phylogenetic diversity, and functional group diversity) affects key N cycling processes in wetland soils across different phenological stages (seedling, fast-growing, reproductive, and wilting). The results showed that multidimensional plant diversity was positively correlated with dissimilatory nitrate reduction to ammonium (DNRA), a N retention process, and denitrification, a N removal process, with greater effects on denitrification.

Spatial transcriptomics reveals Inhba/Smad2/E2f4 axis in Lrp2 thecal cell proliferation in androgen-induced PCOS mice.

Background: Polycystic ovary syndrome (PCOS) is a common endocrine disorder characterized by elevated androgen levels and impaired follicular development. A hallmark of PCOS is the excessive proliferation of thecal cells (TCs), which are critical for androgen production. However, the molecular mechanisms underlying this aberrant cellular expansion remain incompletely understood.

Enhancing Disease-Metabolite Associations Prediction With Weighted Graph Convolutional Networks Based on Community-Driven Link Completion.

Metabolites, the end products of biological processes, hold immense potential as biomarkers for elucidating disease mechanisms. Given the costs associated with wet-lab experiments, computational methods offer a viable solution to narrow the search for candidate metabolites. However, sparse disease-metabolite associations pose a challenge to current deep learning-based methods, limiting comprehensive feature learning.

Effects of Dietary Calcium and Phosphorus Levels on Growth Performance, Calcium-Phosphorus Homeostasis, and Gut Microbiota in Ningxiang Pigs.

Optimal dietary calcium (Ca) and phosphorus (P) requirements remain undetermined for Ningxiang pigs, a valuable indigenous Chinese breed. This study conducted a continuous feeding trial with two growth phases (grower: 30-50 kg; finisher: 50-80 kg) using fixed Ca/P ratios to systematically evaluate the effects of Ca/P levels on growth performance and mineral metabolism. A total of 180 pigs per phase were allocated to four Ca/P levels.

Multi-omics-based study on metabolic-physiological response to heat stress in rainbow trout (Oncorhynchus mykiss).

Global warming poses a critical survival challenge for cold-water economic fish, necessitating elucidation of their physiological and metabolic responses to heat stress. This study established a rainbow trout heat stress model using a gradual temperature increase protocol (from 16 °C to 20 °C over 8 days, followed by maintenance at 20 °C for 22 days) and investigated the adverse effects and adaptive strategies of rainbow trout under heat stress. Extensive congestion and steatosis observed in liver tissues suggested that heat stress induced inflammatory responses and dysregulation of lipid metabolism.

Drying-rewetting legacy mitigates freezing-thawing effects on soil microbial communities and greenhouse gas emissions in plateau riparian wetlands.

Climate change intensifies compound disturbances in soil ecosystems, yet how legacy effects from prior events shape microbial responses to subsequent stresses remains poorly understood. Here, we investigated the legacy effects of drying-rewetting (DW) and freeze-thaw (FT) on greenhouse gas (GHG) emissions and microbial community dynamics across riparian wetlands of the Tibetan Plateau with distinct land-use histories (urban, grazing, and natural). The results showed that urban soils consistently amplified CO emissions compared to grazing and natural lands, whereas natural soils exhibited a pronounced decline in fungal ITS gene abundance, contrasting with the resilience observed in urban and grazing counterparts.

Establishment of Basic-RPA and RPA-LFD Rapid Detection Methods for Ranavirus in Largemouth Bass (Microterus salmoides).

Largemouth bass ranavirus (LMBRaV) disease causes a high mortality rate in largemouth bass aquaculture industry. Establishment of rapid and sensitive virus detection assays is an urgency for prevention of virus transmission and disease outbreak. In this study, basic recombinase polymerase amplification (basic-RPA) and RPA combined with lateral flow dipstick (RPA-LFD) assays for LMBRaV were established to detect virus at low viral loads.

Astragalus polysaccharide regulates the cervical cancer cell cycle and inhibits cisplatin resistance by blocking the Wnt/β-catenin pathway through the PPARD/CDC20 axis.

Unlabelled: Astragalus polysaccharide (APS) can prevent tumor progression and cisplatin resistance. This paper investigates the downstream mechanism of APS in regulating cervical cancer (CC) cisplatin resistance. Cisplatin-resistant CC cell lines were constructed.

The antiviral efficacy of andrographolide against grass carp reovirus in vitro and in vivo.

Grass carp reovirus (GCRV), which causes hemorrhagic disease in grass carp (Ctenopharyngodon idella), poses a significant threat to grass carp aquaculture. To develop effective treatments against GCRV infection, this study evaluated the antiviral efficacy of natural compounds derived from Chinese herbs on GCRV-infected grass carp kidney cells (CIK). Among the various compounds examined, in CIK cells, Andrographolide (Andro) at 25 mg/L exhibited the highest inhibition rates against GCRV-I and GCRV-II infections (83.

Seasonal and Regional Dynamics of the Intestinal Microbiota in from the Nujiang River.

The dynamic alterations in intestinal microbiota can provide insights into the adaptive relationships between these microorganisms and their hosts in response to environmental changes. is widely distributed throughout the Nujiang River and exhibits numerous unique adaptations. In this study, we collected samples of across different seasons and regions within the Nujiang River to comprehensively elucidate the diversity and composition of its intestinal microbiota using metagenomic technology.

Additive Effects of Multiple Global Change Drivers on Terrestrial Nitrogen Cycling Worldwide.

Global change has dramatically altered the Earth's biogeochemical cycles. However, the interactive effects of multiple global change factors (GCFs) on terrestrial nitrogen (N) cycling worldwide remain unclear, limiting the ability to predict how future global change will affect the global N cycle. We conducted a meta-analysis of 108 published articles to evaluate the main and interactive effects of elevated CO, N addition, warming, and altered precipitation on soil N pools (NH , NO , and organic N) and transformation rates (N mineralization, nitrification, and denitrification) across terrestrial ecosystems.

Proteogenomic analysis of Cyprinid herpesvirus 2 using high-resolution mass spectrometry.

Cyprinid herpesvirus 2 (CyHV-2) is the main pathogen responsible for the development of herpesviral hematopoietic necrosis disease (HVHND) in crucian carp (). The CyHV-2 genome encodes approximately 150 genes that are expressed in a well-defined manner during productive infection. However, CyHV-2 open reading frames (ORFs) are primarily derived from sequence and homology analyses, and most lack protein-level evidence to support their properties.

Nanobody based immunoassay for detection of aquatic virus: Giant salamander iridovirus.

Aquatic viruses cause devastating diseases in aquaculture, severely limiting production and resulting in significant economic losses. The giant salamander iridovirus (GSIV), a member of the genus Ranavirus, is the only virus reported to infect giant salamanders, posing a severe threat to the farming industry. Currently, there are no effective strategies available for its control.

Immunological Responses and Protection in the Largemouth Bass () Immunized with Inactivated Vaccine Against Largemouth Bass Ranavirus (LMBRaV).

The largemouth bass ranavirus (LMBRaV) caused significant mortality and economic loss in the largemouth bass aquaculture industry around the world, including China. Vaccination is an efficient method for virus defense. In this study, an inactivated LMBRaV vaccine was prepared, and the prevention effect as well as the immune responses were analyzed after the primary and the secondary immunization.

Diammonium phosphate supplementation in low-protein diets enhances growth performance in growing pigs without compromising carcass traits and meat quality.

Optimizing low-protein (LP) diets in swine nutrition is critical for reducing nitrogen excretion and resource waste while reducing meat quality. However, LP diets may disrupt amino acid (AA) balance, affecting growth and health. Supplementing with nonprotein nitrogen sources such as diammonium phosphate (DP) can enhance nitrogen utilization and support protein synthesis efficiently.

Global meta-analysis deciphering ecological restoration performance of dredging: Divergent variabilities of pollutants and hydrobiontes.

Global "Sustainable Development Goals" propose ambitious targets to protect water resource and provide clean water, whereas comprehensive understanding of restoration performance and ecological mechanisms are lacking for dredging adopted for purifying polluted waterbodies and maintaining navigation channels. Here, we conducted a global meta-analysis to estimate ecological restoration consequence of dredging as pollution mitigation and navigation channel maintenance measures using a dataset compiled from 191 articles covering 696 studies and 84 environmental and ecological parameters (e.g.

α-Ketoglutarate modulates the mechanisms of toxicity in crucian carp kidneys chronically exposed to NaHCO: Metabolomics insights.

Due to the unique physicochemical properties of saline-alkaline water, aquatic organisms find it challenging to survive under such conditions. This study established a freshwater control group (C), a 20 mmol/L NaHCO exposure group (T), a 40 mmol/L NaHCO exposure group (F), a 20 mmol/L NaHCO exposure with α-ketoglutarate (AKG)-supplemented feed group (TA), and a 40 mmol/L NaHCO exposure with AKG-supplemented feed group (FA). Histopathological analysis, biochemical assays, and UPLC-QTOF/MS metabolomics were employed to explore the potential mechanisms by which AKG alleviates oxidative damage in freshwater teleosts induced by saline-alkaline stress.

Integration of microbiomics and metabolomics reveals energy metabolism imbalance in crucian carp (Carassius auratus) under saline-alkaline exposure.

The ecological conditions of freshwater aquaculture are deteriorating by degrees in recent years. Consequently, the comprehensive utilization of saline-alkaline water has garnered increasing societal attention. Here, crucian carp (Carassius auratus) were exposed to 20, 40 mmol/L NaHCO for 30 days (T, F group).

Mechanism of blocking the glutamate pathway to exacerbate oxidative stress, ammonia toxicity and metabolic disorders in crucian carp (Carassius auratus) under saline-alkaline exposure.

Climate change and intensified human activities have accelerated the salinization and alkalinization of aquatic environments, further shrinking the space for freshwater aquaculture. One of the key survival mechanisms for fish in saline-alkaline habitats is the conversion of accumulated endogenous ammonia into less toxic glutamine. This study focuses on the freshwater teleost, crucian carp (Carassius auratus), using the liver as the target organ.

Immunoglobulin M response in largemouth bass () following ranavirus infection.

Immunoglobulin M (IgM) and IgM B cells are key components of the humoral immune system, providing defense against pathogen invasion. While the role of IgM in the systemic and mucosal immune responses of fish to parasites and bacteria has been partially investigated, its function in viral infections remains underexplored. This study successfully developed a largemouth bass () model for ranavirus immersion infection.

Difficult airway management in 25 hospitals across China: A multicenter cross-sectional study.

Study Objective: Difficult airway management is a significant challenge in clinical anesthesia, critical care, and emergency medicine. Inadequate management can lead to severe complications including organ damage and death. This study assessed the variability in difficult airway management across China and focused on how patient and operator factors influenced outcomes in operating rooms.

Exploring Disparities in Gill Physiological Responses to NaHCO-Induced Habitat Stress in Triploid and Diploid Crucian Carp (): A Comprehensive Investigation Through Multi-Omics and Biochemical Analyses.

: Owing to the progressive rise in saline waters globally, resulting in detrimental impacts on freshwater aquaculture, the underlying molecular distinctions governing the response to alkaline stress between diploid and triploid crucian carp remain unknown. : This investigation explores the effects of 20 and 60 mmol NaHCO stress over 30 days on the gills of diploid and triploid crucian carp, employing histological, biochemical, and multi-omic analyses. : Findings reveal structural damage to gill lamellas in the examined tissue.