The clinic-in-a-dressing paradigm: Synchronized diagnosis and treatment in chronic wounds via hydrogel-nanofiber matrices.

Chronic wounds, a critical global health challenge, necessitate advanced solutions to address limitations in current care, such as subjective assessments, uncontrolled drug delivery, and poor integration of diagnosis and treatment. This study introduces a "clinic-in-a-dressing" system that combines in situ pH monitoring with dual-responsive (pH/enzyme) drug delivery for chronic wound management. The system integrates hyaluronic acid (HA)-encapsulated vancomycin-loaded zeolitic imidazolate framework (HZV) nanoparticles and fluorescein isothiocyanate (FITC) into a nanofiber-reinforced hydrogel sponge.

Multifunctional Nanobody Fusion Proteins in Immunoassays: Diverse Strategies for Enhanced Analytical Performance.

Nanobodies, derived from camelid heavy-chain antibodies, offer exceptional specificity, stability, and solubility, enabling rapid and sensitive immunoassays. Their small size makes them ideal scaffolds for genetic fusion with reporter proteins, functional peptides, and signal amplification modules. In contrast to conventional antibodies, nanobodies can be precisely engineered at the gene level, allowing directional conjugation and efficient incorporation of signal-enhancing elements.

Social Connection and Chronic Pain: A Cohort Study to Explore the Association of Social Isolation and Loneliness with Chronic Pain Among Older Adults in China.

Background: As societies worldwide experience rapid aging, social isolation and loneliness are as prevalent and impactful on health outcomes of older adults as other well-recognized risk factors. This study investigates the association of social isolation and loneliness on the prevalence of chronic pain among Chinese older adults.

Methods: Participants from waves of the Chinese Health and Retirement Longitudinal Study (CHARLS) with no chronic pain in baseline 2011, and their conditions of chronic pain in wave 2018, were analyzed.

Heterogeneous saltwater intrusion patterns and drivers among multiple outlets in a complex estuary.

Estuary saltwater intrusion presents a global challenge to ecosystem health and river water intake for human usages. The main influencing factors include river runoff, tide, and wind, and the dynamics of these factors can be characterized by magnitude, variability, frequency, timing, and extreme. These characteristics combine to determine spatiotemporal saltwater intrusion patterns.

Inhibition of ADAM17: A New Therapeutic Strategy to Alleviate Zearalenone Toxin-Induced Liver Injury by Endoplasmic Reticulum Stress and Inflammation.

Zearalenone (ZEA) is a mycotoxin that heavily contaminates feed and its raw materials, entering the body through dietary intake. Although it has been reported that ZEA causes liver damage, its toxic mechanisms are unclear. In this study, we first confirmed that ZEA induces liver injury in mice, triggering endoplasmic reticulum stress (ERS) and inflammation .

Nanoplastics Drive the Charge-specific Decline of Aquatic Insect () Emergence through Inducing Oxidative Damage and Perturbing the Endocrine System.

The ongoing decline in the emergence and biomass of aquatic insects is partly due to the wide distribution of engineered nanoparticles. Nanoplastics (NPs), as emerging engineered nanoparticles, can cause toxicity to aquatic organisms. However, the mechanisms underlying the effects of NPs on aquatic insect emergence are not clear.

Multi-omic insights from a multi-ancestry genome-wide meta-analysis of ankylosing spondylitis reveal novel pathways of disease susceptibility.

We report the largest genome-wide association study meta-analysis in ankylosing spondylitis (AS) to date (25,645 cases, 71,224 controls), identifying 27 novel loci and 86 independent genetic associations. Variations in (non-secretor status) and (blood group A) increase AS risk, with Mendelian randomisation (MR) linking non-secretor status to increased AS risk from reduced gut carriage of . Associations with three telomerase maintenance genes (, ), and MR analysis, suggest increased telomere length causally increases AS susceptibility.

Zearalenone toxin induces pyroptosis by activating mitochondrial DNA-STING-NFκB axis in testis and TM4 cell damage.

Zearalenone (ZEA), a common mycotoxin in grains and co-products, poses significant reproductive toxicity. However, the toxicity mechanism between ZEA and reproductive toxicity remains unclear. Mitochondrial DNA (mt DNA) alteration is considered to be an important factor in sterility.

Effects of Ganoderma spent substrate containing with polysaccharides and triterpenoids on the growth performance, antioxidant capacity and immunity level of chickens.

Ganoderma spent substrate (GSS) is the residual material of Ganoderma lucidum (GL) cultivation, which has abundant polysaccharides and triterpenoids. GL is widely recognized for its antioxidant and immune-boosting properties, however, the effects of GSS remain largely unexplored. This study aimed to investigate the effects of GSS on growth performance, antioxidant capacity, and immune function in chickens.

Development of Immunoassays for Foodborne Pathogenic Bacteria Detection Using PolyHRP for Signal Enhancement.

The rapid and accurate detection of foodborne pathogens is essential for ensuring food safety. O157:H7 ( O157:H7) and () are major foodborne pathogenic bacteria that pose significant public health risks, highlighting the need for effective detection methods. In this study, highly sensitive double-antibody sandwich-based enzyme-linked immunosorbent assays (ELISAs) were developed for the rapid detection of O157:H7 and , utilizing a streptavidin-polymerized horseradish peroxidase (SA-PolyHRP)-based signal enhancement system.

Diagnostic imaging confusion in infectious spondylitis.

Background: Infectious spondylitis is a rare but increasingly recognized condition involving infectious lesions of the spine. It can affect patients of all ages and is typically caused by bacterial, fungal, or other pathogens. The infection commonly targets the vertebral bodies and intervertebral discs.

Electroactive FeO/α-FeO@Au nanocomposites driven label-free electrochemical aptasensor with magnetic self-assembly for rapid quantification of alpha-fetoprotein.

Alpha-fetoprotein (AFP) is a crucial biomarker for several cancers' diagnosis, especially hepatocellular carcinoma; therefore, early accurate detection of AFP is of vital significance. Herein, a label-free electrochemical aptasensor with magnetic self-assembly of heterogeneous FeO/α-FeO nanosheets is presented for rapid and accurate detection of AFP. The sensor was mainly designed by loading Au nanoparticles (AuNPs) on the surface of FeO/α-FeO nanosheets and further binding with aptamer probes through Au-S bonds, and the target could be captured by the high affinity and specificity with the aptamer.

Regional lung function assessment using electrical impedance tomography in COPD, PRISm, and normal spirometry subjects: insights into early diagnostic potential.

Purpose: This study utilizes electrical impedance tomography (EIT) to explore spatial-temporal heterogeneity in regional lung function among patients with chronic obstructive lung disease (COPD), preserved ratio impaired spirometry (PRISm), and those with normal lung function.

Methods: Subjects who had pulmonary function test at Sir Run Run Shaw Hospital from 28 December 2023 to 30 March 2024 were screened. Regional lung functions were accessed with EIT regarding spatial distribution, abnormal area size, and expiratory time.

Sorting Extrusion-Prepared Coreless Cell Membrane Vesicles of Mixed Orientations by Functional DNA Probes.

Cell membrane vesicles (CMVs) have been extensively used as delivery vehicles for a variety of cargos, which are generally prepared via membrane extrusion. Extruded CMVs are not necessarily to have the outer membrane facing outward due to the randomness of membrane wrapping. Nanoparticles have been used to serve as cores to direct the membrane orientation of CMVs; nevertheless, there is a lack of methods to efficiently sort coreless CMVs of desired orientations.

Inverse binary optimization of convolutional neural network in active learning efficiently designs nanophotonic structures.

Binary optimization using active learning schemes has gained attention for automating the discovery of optimal designs in nanophotonic structures and material configurations. Recently, active learning has utilized factorization machines (FM), which usually are second-order models, as surrogates to approximate the hypervolume of the design space, benefiting from rapid optimization by Ising machines such as quantum annealing (QA). However, due to their second-order nature, FM-based surrogate functions struggle to fully capture the complexity of the hypervolume.

Genome-wide CRISPR screening identifies LRP1 as an entry factor for SFTSV.

Severe fever with thrombocytopenia syndrome (SFTS) is an emerging tick-borne disease caused by the SFTS virus (SFTSV), which has high mortality rates and poses a significant threat to public health. To identify potential therapeutic targets against SFTSV, we conduct genome-wide knockout screening, which identifies the previously known host factor CCR2, and reveals prolow-density lipoprotein receptor-related protein 1 (LRP1) as an entry factor for SFTSV. Knockdown or knockout of LRP1 significantly attenuate SFTSV infection in mouse embryonic fibroblasts (MEFs).

Visual Detection and Identification of Influenza A Viruses by Nucleic Acid Probe-Enabled Lateral Flow Assay.

Diagnosis of influenza A viral infection is crucial for preventing disease transmission and providing effective clinical treatments. There is an increasing need for convenient detection methods to enable simple yet precise identification of viral infections. Herein, a nucleic acid probe-enabled lateral flow assay (NALFA) is developed to realize visual detection and identification of influenza A viral infections (H1N1 and H3N2) of high sensitivity and specificity.

Crystal Facet Regulation and Photocatalytic Properties of α-FeOOH: Crystal Facet Effects on the Built-In Electric Fields Coupled with Coordination Adsorption.

The α-FeOOH (021) crystal facet has a high density of active sites, and as a reactive facet, it has good adsorption of pollutants and good photocatalytic performance. In this paper, nano α-FeOOH with different (021)/(110) crystal facet ratios was prepared using crystal facet inducers (ethanol, ethylene glycol, and glycerol) via a hydrothermal method, with a (021)/(110) crystal facet ratio reaching 16% in the FG-2 sample. The results of photocatalytic experiments show that this sample's removal rate of Cr(VI) reaches 60.

Radiotherapy promotes cuproptosis and synergizes with cuproptosis inducers to overcome tumor radioresistance.

Cuproptosis is a recently identified form of copper-dependent cell death. Here, we reveal that radiotherapy (RT) induces cuproptosis in cancer cells, independent of apoptosis and ferroptosis, and depletes lipoylated proteins and iron-sulfur (Fe-S) cluster proteins-both hallmarks of cuproptosis-in patient tumors. Mechanistically, RT elevates mitochondrial copper levels by upregulating copper transporter 1 (CTR1) and depleting mitochondrial glutathione, a copper chelator, thereby triggering cuproptosis.

The development of pyridazinone-based andrographolide derivatives as anti-cancer agents with the ability of inhibiting the TFAP4/Wnt/β-catenin signaling pathway.

Emerging evidence indicated that natural andrographolide and its derived compounds could exert anti-cancer effects on a broad range of cancer cells by several mechanisms of actions. However, the potent andrographolide derivatives with novel structures are needed and the comprehensive understanding of the underlying mechanisms of actions are still lacking. In this work, we reported the pyridazinone-based anti-cancer andrographolide derivative A61, which is superior to the widely-used anti-cancer drug 5-FU (around 5-fold more potent), it showed high potency to inhibit the growth and migration of a panel of cancer cells, in which the gastric cancer cells exhibited the highest drug sensitivity.

Corrigendum: spondylitis in an immunocompetent patient with annular high signal around the intervertebral disks: a case report and literature review.

[This corrects the article DOI: 10.3389/fmed.2024.

Nanobody based immunoassay for detection of aquatic virus: Giant salamander iridovirus.

Aquatic viruses cause devastating diseases in aquaculture, severely limiting production and resulting in significant economic losses. The giant salamander iridovirus (GSIV), a member of the genus Ranavirus, is the only virus reported to infect giant salamanders, posing a severe threat to the farming industry. Currently, there are no effective strategies available for its control.

Nanobody-Based Immunoassays for the Detection of Food Hazards-A Review.

Food safety remains a significant global challenge that affects human health. Various hazards, including microbiological and chemical threats, can compromise food safety throughout the supply chain. To address food safety issues and ensure public health, it is necessary to adopt rapid, accurate, and highly specific detection methods.

Transcriptome analysis reveals that the injection of mesenchymal stem cells remodels extracellular matrix and complement components of the brain through PI3K/AKT/FOXO1 signaling pathway in a neuroinflammation mouse model.

Neurological disorders are often accompanied by neuroinflammatory responses. Our previous research indicated that mesenchymal stem cells (MSCs) suppressed neuroinflammation in the brain. The mechanism of action remains not fully understood.