The mRNA vaccine encoding Gc protein confers complete protection against severe fever with thrombocytopaenia syndrome virus.

Severe fever with thrombocytopaenia syndrome virus (SFTSV), an emerging tick-borne pathogen, causes haemorrhagic fever in infected patients and is associated with a high mortality rate in humans. The imperative need for vaccines against this lethal virus is underscored by a lack of effective preventive measures. The results of this study yield notable advancements: the successful development of an SFTSV mRNA vaccine encoding the glycoprotein C (Gc) gene, achieving N-linked glycosylation in the expressed protein.

Efficacy and safety of modified combined dorsal-volar approach for internal fixation in high-energy trauma-induced comminuted distal radius fracture with concomitant scaphoid fracture.

This study investigated the clinical outcomes of the modified combined dorsal-volar approach for internal fixation of comminuted distal radius fractures complicated by scaphoid fractures resulting from high-energy trauma. A cohort of 100 patients with concurrent distal radius and scaphoid fractures underwent surgical management by this approach. Postoperative assessments included wrist joint function recovery, operative parameters (surgical time, intraoperative blood loss, and hospital stay), range of motion (ROM), functional scores (Disabilities of the Arm, Shoulder, and Hand [DASH], Bartra, and Gartland-Werley), fracture healing-related indicators, and serum inflammatory cytokine levels.

Topography-based implants for bone regeneration: Design, biological mechanism, and therapeutics.

With the increasing demand for bone defect repair, bone implant materials have emerged as a critical alternative to traditional autologous or allogeneic bone grafts. However, their clinical performance remains limited due to challenges such as prolonged healing times and suboptimal repair quality. Moreover, in patients with certain pathological conditions (e.

Development of histological sectioning techniques for the cochlear implanted inner ear in miniature swine.

Background: Cochlear pathological sectioning is essential for studying inner ear structural changes. Traditional methods like paraffin embedding, frozen sectioning, and collodion embedding are limited to decalcified tissues. For cochlear specimens with rigid implants (e.

Precursors of Carboximidates Deriving from Thioamide-Trimethyl-Lock System: A General Method for Designing Stable Cascade Acid-Sensitive Prodrugs.

We report a cascade acid-sensitive prodrug design strategy utilizing a thioamide-trimethyl-lock system. This approach successfully balances prodrug stability and high acid sensitivity, enabling precise drug release in acidic microenvironments. The thioamide system further acts as a hydrogen sulfide (HS) donor, providing organ-protective effects.

Effects of salinity stress on morphological structure, physiology, and mRNA expression in different wheat ( L.) cultivars.

Salinity is a major abiotic stress that threatens crop yield and food supply in saline soil areas. Wheat ( L.) is the most important cereal crop in arid and semiarid land areas, which are often adversely affected by soil salinity.

Bimetallic nanoparticles encapsulated within a graphitized nitrogen-doped carbon shell for efficient pH-universal electrochemical carbon dioxide reduction.

The large-scale production of carbon monoxide (CO) through electrochemical carbon dioxide (CO) reduction reaction (eCORR) is a promising strategy for mitigating CO emissions and energy crisis. However, developing high-efficiency, cost-effective, stable, and pH-universal electrocatalysts for eCORR is of utmost urgency. In this study, nickel-zinc bimetallic nanoparticles encapsulated within a graphitized nitrogen-doped carbon shell (NiZn-NiZnC@NC) were synthesized and used as robust electrocatalysts for the eCORR-to-CO process.

CoNi Alloy Nanoparticles Encapsulated Within N-Doped Carbon Nanotubes for Efficiently pH-Universal CO Electroreduction.

The large-scale production of carbon monoxide (CO) through electrochemical CO reduction reaction (CORR) represents a promising strategy for mitigating CO emissions and energy crisis. However, the development of high-efficiency, stable, and pH-universal electrocatalysts for CORR is of utmost urgency. In this study, CoNi alloy nanoparticles encapsulated within N-doped carbon nanotubes (N-CNTs) are synthesized and employed as robust catalysts toward CORR-to-CO.

Long non-coding RNA HOXA11-AS inhibits apoptosis, induces proliferation, and promotes autophagy via the miR-214-3p/ATG12 axis in acute T lymphoblastic leukemia.

Acute T lymphocytic leukemia (T-ALL) is a hematological cancer with high mortality. The literature suggests an association between T-ALL and Long non-coding RNAs (lncRNAs). Nevertheless, the mechanism of lncRNA HOXA11-AS in T-ALL remains undetermined.

Bonding and Adhesion Mechanisms for Laser Transmission Welding of Thermoplastic Polymers in Liquid Flow Batteries.

Adhesion and bonding are critical to the success of polymer composites, particularly in the sealing processes of redox flow batteries, where laser transmission welding technology for plastics is beginning to gain traction. However, the key factors influencing welding and microscale bonding mechanisms remain incompletely understood. In this article, we systematically analyzed the optimal process parameters and patterns for laser welding of polypropylene (PP) plastics using orthogonal experiments and investigated the interfacial bonding mechanisms through morphology analysis and fracture force testing.

Reclassification of variants of uncertain significance in neonatal genetic diseases: implications from a clinician's perspective.

Although whole-exome sequencing (WES) is now widely used to diagnose neonatal genetic diseases, the genetic causes in over half of the cases remain unresolved, primarily due to variants of uncertain significance (VUS). Therefore, reclassifying VUS may be an effective strategy to improve WES's diagnostic yield. However, not all reclassification approaches are suitable for clinicians.

Oral exacerbates ulcerative colitis via the oral-gut axis: mechanisms and therapeutic implications.

Background: () is an anaerobic bacterium known for its association with periodontal disease and oral infections. It has been implicated in the development of gastrointestinal diseases such as inflammatory bowel disease and colorectal cancer. Ulcerative colitis (UC), which is characterized by chronic inflammation of the colon, is a condition of unknown etiology with a rising incidence rate, significantly affecting the quality of life for patients.

Alumina nanoparticles induce learning and memory impairment in a particle size-dependent and time-dependent manner.

The study investigates the influence of alumina nanoparticles (AlO NPs) at varying sizes on the learning and memory of adult ICR mice over different exposure durations. The mice were administered saline or AlO nanoparticles of 10μm, 50 nm, and 13 nm via nasal drip. Following administration, the Morris water maze test was conducted, along with assessments of inflammation, oxidative stress, hippocampal histopathology, and cell death-related proteins.

An ultra-high-performance tandem mass spectrometry method to quantify tryptophan metabolites in aqueous humor of primary angle-closure glaucoma patients.

This study presented the development and validation of a robust ultra-performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS) method for the simultaneous quantification of tryptophan (TRP) and its nine metabolites in aqueous humor (AH) to explore the regulation of the TRP metabolic pathway in primary angle-closure glaucoma (PACG). The optimized UPLC-MS/MS method demonstrated good linearity (R² > 0.99), sensitivity (LLMI: 0.

Minimizing hearing loss: A novel surgical approach for round window exposure in miniature pigs.

Background: A delicate operation for exposing the round window without affecting the hearing function is critical for auditory research. Despite the different surgical approaches proposed for miniature pigs, hearing protection during operation is still challenging. The efficient solution to this problem is significant for accurate auditory research.

Polydopamine Nanocomposite Hydrogel for Drug Slow-Release in Bone Defect Repair: A Review of Research Advances.

In recent years, hydrogels have emerged as promising candidates for bone defect repair due to their excellent biocompatibility, high porosity, and water-retentive properties. However, conventional hydrogels face significant challenges in clinical translation, including brittleness, low mechanical strength, and poorly controlled drug degradation rates. To address these limitations, as a multifunctional polymer, polydopamine (PDA) has shown great potential in both bone regeneration and drug delivery systems.

Mechanical stimulation of cochlea for treatment of hearing loss: Comparison between forward stimulation and reverse stimulation with an active cochlear model.

Background And Objective: Reverse stimulation is a stimulation mode of the active middle-ear implants (AMEIs), targeted at moderate conductive hearing loss and mixed hearing loss. However, previous studies investigated reverse stimulation through passive cochlear models that simulate profound sensorineural hearing loss, which is beyond the AMEI's indications. Therefore, we investigated the cochlear responses to reverse stimulation under different hearing loss and compared them with those to forward stimulation.

A thiocoumarin based self-reporting sulfide prodrug strategy with a favorable safety profile.

HS as the third gasotransmitter is an important endogenous bioregulator that shows various therapeutic potentials. Herein, we present a novel thiocoumarin-based self-reporting sulfide prodrug strategy that utilizes esterase-mediated hydrolysis of thionoesters to release HS and provide real-time fluorescence monitoring. Our key discovery is that thionoesters can be hydrolyzed by esterases to release HS under physiological conditions, providing ample opportunities to design prodrugs based on ester-containing molecules.

The influence of narrative pedagogy on academic achievement and humanistic care ability of Chinese nursing students: A systematic review and meta-analysis.

Aim: This study aims to compare the effects of narrative pedagogy with traditional teaching methods on nursing education outcomes.

Background: Narrative pedagogy has been developed for over a decade, and while studies confirm that it can stimulate student interest, foster empathy, and enhance the integration of theoretical knowledge with practical skills in nursing care, there is still insufficient evidence to recommend its widespread adoption.

Methods: In November 2024, a comprehensive search was conducted across databases including PubMed, Embase, The Cochrane Library, Web of Science, OVID, Scopus, PsychInfo, CNKI, Wanfang Data, Chinese Science and Technology Periodical Database (VIP) and China Biology Medicine disc (CBM) to identify studies that met the inclusion and exclusion criteria.

Homobifunctional Photocaged 2-Butene-1,4-dials (bis-PBDAs) as Amino-Reactive Photo-Crosslinkers for Biomaterial Fabrication and Cell Fixation.

Crosslinking amine-rich biomolecules with aldehydes, such as paraformaldehyde (PFA) and glutaraldehyde (GA), is a widely used method for biomaterial fabrication and cell fixation. In this study, we introduce homobifunctional photocaged 2-butene-1,4-dials (bis-PBDAs) as novel amine-reactive photo-crosslinkers. Compared to PFA and GA, bis-PBDAs are inherently less toxic.

Abnormal characteristics of inferior vena cava and abdominal aorta among neonates with early onset septic shock.

Background: The variety of shocks in neonates, if not recognized and treated immediately, is a major cause for fatality. The use of echocardiography may improve assessment and treatment, but its reference values across gestational age (GA) and birth weight (BW) are lacking. To address the information gap, this study aimed at correlating GA and BW of newborns with nonhemodynamic abnormalities, and at evaluating the usefulness of such reference values in neonates with early onset septic (EOS) -shock.

Different Flooding Conditions Affected Microbial Diversity in Riparian Zone of Huihe Wetland.

The soil microbiome plays an important role in wetland ecosystem services and functions. However, the impact of soil hydrological conditions on wetland microorganisms is not well understood. This study investigated the effects of wetted state (WS); wetting-drying state (WDS); and dried state (DS) on the diversity of soil bacteria, fungi, and archaea.

Multiscale Hierarchical Micro- and Nano-Surface Induced by High-Repetition-Rate Femtosecond Laser Promote Peri-Implant Osseointegration.

Micro- and nanomorphological modification and roughening of titanium implant surfaces can enhance osseointegration; however, the optimal morphology remains unclear. Laser processing of implant surfaces has demonstrated significant potential due to its precision, controllability, and environmental friendliness. Femtosecond lasers, through precise optimization of processing parameters, can modify the surface of any solid material to generate micro- and nanomorphologies of varying scales and roughness.

HCAR2 Modulates the Crosstalk between Mammary Epithelial Cells and Macrophages to Mitigate Staphylococcus aureus Infection in the Mouse Mammary Gland.

Staphylococcus aureus (S. aureus) is a major zoonotic pathogen, with mammary gland infections contributing to mastitis, a condition that poses significant health risks to lactating women and adversely affects the dairy industry. Therefore, understanding the immune mechanisms underlying mammary infections caused by S.

Animal models of cochlear implant: Classification and update.

Cochlear implantation (CI) is currently recognized as the most effective treatment for severe to profound sensorineural deafness and is considered one of the most successful neural prostheses. Since its inception in 1961, cochlear implantation has expanded its range of applications to encompass younger newborns, older people, and individuals with unilateral hearing loss. In addition, it has improved its surgical methods to minimize the occurrence of complications.