[Comparison on chemical components of Angelicae Sinensis Radix before and after wine processing by HS-GC-IMS, HS-SPME-GC-MS, and UPLC-Q-Orbitrap-MS combined with chemometrics].

The study investigated the intrinsic changes in material basis of Angelicae Sinensis Radix during wine processing by headspace-gas chromatography-ion mobility spectrometry(HS-GC-IMS), headspace-solid phase microextraction-gas chromatography-mass spectrometry(HS-SPME-GC-MS), and ultra-high performance liquid chromatography-quadrupole-orbitrap mass spectrometry(UPLC-Q-Orbitrap-MS) combined with chemometrics. HS-GC-IMS fingerprints of Angelicae Sinensis Radix before and after wine processing were established to analyze the variation trends of volatile components and characterize volatile small-molecule substances before and after processing. Principal component analysis(PCA) and orthogonal partial least squares-discriminant analysis(OPLS-DA) were employed for differentiation and difference analysis.

Basophil-derived exosomes exacerbate systemic lupus erythematosus by regulating B-cell proliferation via miR-24550.

Background: Systemic lupus erythematosus (SLE) is a complex autoimmune disease where B-cell proliferation and activation play a pivotal role in pathogenesis. While the role of basophils in SLE is recognized, the impact of basophil-derived exosomes on B-cell proliferation and activation has not been thoroughly investigated.

Methods: Exosomes from human basophils in both resting and activated states were isolated and characterized.

A fluorescent aptasensor for accurate and sensitive detection of glyphosate based on asymmetrically competitive CRISPR/Cas12a with phosphorothioate-modified G-quadruplex.

Accurate detection of glyphosate (GLY) residues is essential for protecting public health, ensuring food safety, and preventing environmental pollution caused by excessive or improper herbicide application. Based on the asymmetric CRISPR/Cas12a system as a signal amplification technique, with phosphorothioate-modified hairpin G-quadruplex (psHG4) acting as a signal probe, a platform named ACC-GLY is developed for the sensitive and accurate detection of GLY. In the designed ACC-GLY platform, the target GLY specifically binds to the aptamer, and the cascading signal amplification strategy, driven by the DNase activity of a single Cas12a, is initiated by two competitive guide RNAs.

Homologous targeted neutrophils-liposome system for pyroptosis-enhanced antitumor immunotherapy of triple-negative breast cancer.

Triple-negative breast cancer (TNBC), characterized by negative expression of estrogen receptor, progesterone receptor, and oncogene HER-2, always exhibits resistance to targeted drugs. Immunotherapies have demonstrated promising clinical responses and significant potential for TNBC treatment. Enhancing and activating antitumoral immunity can help restrain TNBC progression.

Gap-free genome and efficient transcript purification system reveals the genomes diversity and chlorophyll degradation mechanism in pitaya.

Pitaya is an important perennial herbaceous fruit tree. The color of fruit determines pitaya nutritive (and attractive) value, which is considered as an important objective in breeding improvement. In this study, we reported the first telomere-to-telomere (T2T) gap-free genome of "Shuangse No.

Biosynthesis of Silver Nanoparticles Using Endophytic Clonostachys rosea ZMS27 from Medicinal Plant Anemarrhena asphodeloides and its Antagonistic Activity Against Superficial Pathogenic Fungi.

Superficial fungal infections (SFIs) represent a serious threat to global public health. It is known that silver nanoparticles (AgNPs) are characterized by potent antimicrobial properties. However, the therapeutic potential of biosynthetic AgNPs on superficial fungi have rarely been reported.

Basophilic exosomes promote SLE development via exacerbating B cell activation through the lncRNA ENST00000537616/miR-330-5p/KRAS axis.

Introduction: Uncontrolled B-cell activation in systemic lupus erythematosus (SLE) induces autoantibody production and inflammation and causes tissue damage. Basophils have been shown to promote B cell activation in SLE; however, the molecular mechanisms involved remain nebulous.

Objective: To elucidate the role of basophilic exosomes in B-cell activation and explore the associated molecular mechanisms in SLE.

MindGPT: Interpreting What You See With Non-Invasive Brain Recordings.

Decoding of seen visual contents with non-invasive brain recordings has important scientific and practical values. Efforts have been made to recover the seen images from brain signals. However, most existing approaches cannot faithfully reflect the visual contents due to insufficient image quality or semantic mismatches.

Design, synthesis, and biological evaluation of C-12 modified ocotillol-type derivatives as novel P-glycoprotein modulators for overcoming multidrug resistance.

Ocotillol-type ginsenoside derivatives exhibit significant potential as modulators of P-glycoprotein (Pgp). To date, structural investigations of Ocotillol-type saponins have predominantly focused on modifications at the C-3 position of the A-ring, with limited exploration of the C-12 position on the C-ring. In this study, we designed and synthesized a series of C-12 modified ocotillol-type derivatives and assessed their efficacy in reversing multidrug resistance (MDR) in KBV cells.

Self-assembling protein nanoparticles for cytosolic delivery of nucleic acids and proteins.

Intracellular delivery of biomacromolecules is hampered by low efficiency and cytotoxicity. Here we report the development of elastin-based nanoparticles for therapeutic delivery (ENTER), a recombinant elastin-like polypeptide (ELP)-based delivery system for effective cytosolic delivery of biomacromolecules in vitro and in vivo. Through iterative design, we developed fourth-generation ELPs fused to cationic endosomal escape peptides (EEPs) that self-assemble into pH-responsive micellar nanoparticles and enable cytosolic entry of cargo following endocytic uptake.

Integrated analyses of the transcriptome and metabolome revealed the coloring mechanism of red-pericarp wampee (Clausena lansium).

Wampee (Clausena lansium), a tropical evergreen fruit from the Rutaceae family renowned for its rich nutrient profile and bioactive compounds, presents a fascinating case study in fruit coloration. However, changes in anthocyanins, and expressions of metabolism-associated genes during fruit maturation of red-pericarp wampee ('ZR') are not documented. In this study, metabolic and gene expression profiles of anthocyanin across different fruit developmental stages of red and yellow-pericarp wampees were analyzed.

The protein deacetylase HDAC10 controls DNA replication in malignant lymphoid cells.

Histone deacetylases (HDACs) comprise a family of 18 epigenetic modifiers. The biologically relevant functions of HDAC10 in leukemia cells are enigmatic. We demonstrate that human cultured and primary acute B cell/T cell leukemia and lymphoma cells require the catalytic activity of HDAC10 for their survival.

Phosphorothioate-Modified Hairpin G-Triplex Reporter-Assisted Split CRISPR/Cas12a-Powered Biosensor for "Turn-On" Fluorescent Detection of Nucleic Acid and Non-Nucleic Acid Targets.

CRISPR/Cas12a-powered biosensors with guanine (G)-rich sequence reporters (e.g., G-quadruplex and G-triplex) are widely used in detection applications due to their simplicity and sensitivity.

Ratiometric Dual-Response Quantum Dot Spherical Nucleic Acid for Monitoring Viral Secondary Bacterial Infections.

Viral-bacterial coinfections present intricate pathologies that exacerbate disease progression and elevate mortality rates. Understanding the dynamic interplay between viruses and bacteria during coinfection is critical for developing effective therapeutic interventions. However, current diagnostic tools primarily rely on static detection methods, limiting their ability to monitor real-time infection dynamics.

Exploring the role of gut microbiota modulation in the long-term therapeutic benefits of early MSC transplantation in MRL/lpr mice.

Background: Systemic lupus erythematosus (SLE), influenced by gut microbiota dysbiosis, is characterized by autoimmune and inflammatory responses. Human umbilical cord-derived mesenchymal stem cell (hUC-MSC) transplantation is an effective and safe treatment for refractory or severe SLE; however, the long-term efficacy and mechanisms of early hUC-MSC therapeutic benefits in SLE need further investigation.

Methods: Here, lupus-prone MRL/MpJ-Fas (MRL/lpr) mice were divided into three groups: the control (Ctrl) group received saline injections, while the MSC and MSC-fecal microbiota transplantation (FMT) groups received early hUC-MSC transplants at weeks 6, 8, and 10.

Codelivery of apigenin, FdUMP and CD276 antibody synergistic inhibit colorectal cancer by ferroptosis-apoptosis-pyroptosis and CD276 blockade.

Mitochondria provides adenosine triphosphate for multiple vital movements to ensure tumor cell proliferation. Compared to the broadly used method of inducing DNA replication arrest to kill cancer, inducing mitochondria damage to cause energy shortage is quite promising as it can inhibit tumor cell bioactivities, increase intracellular accumulation of toxic drugs, eventually sensitize chemotherapy and even reverse drug resistance. Breaking the balance of glutathione (GSH) and reactive oxygen species (ROS) contents have been proven efficient in destroying mitochondria respectively.

Analysis of Volatile Organic Compounds Differences of Black and White Pepper by HS-GC-IMS and HS-SPME-GC-MS.

Rationale: As a medicinal and edible herb, Piper nigrum L. is abundant in volatile organic compounds (VOCs), and its essential oil has antibacterial properties. Notably, the aromatic profiles of black pepper (BP) and white pepper (WP) are markedly distinct.

Temporal dynamics of nutrient release from mulching of legume roots and shoots litter driven by microbial community during decomposition in organic orchards.

Grass residue decomposition is crucial for nutrient cycling in agro-ecosystems, enhancing nutrient utilization efficiency and supporting sustainable crop management. While grass mulching has been widely studied for improving orchard soil fertility, the role of soil microbial communities in decomposing different plant organs remains unclear. Before decomposition, the aboveground and belowground plant parts were harvested and placed in separate litterbags, which were later used for evaluating the decomposition rate and chemical characteristics of the shoots and roots for 40 days (at 10 days intervals).

A thiocoumarin based self-reporting sulfide prodrug strategy with a favorable safety profile.

HS as the third gasotransmitter is an important endogenous bioregulator that shows various therapeutic potentials. Herein, we present a novel thiocoumarin-based self-reporting sulfide prodrug strategy that utilizes esterase-mediated hydrolysis of thionoesters to release HS and provide real-time fluorescence monitoring. Our key discovery is that thionoesters can be hydrolyzed by esterases to release HS under physiological conditions, providing ample opportunities to design prodrugs based on ester-containing molecules.

Assessment of sulfate and nitrate variations in China during 19902020: Insights into source contributions and formation pathways.

Sulfate (SO) and nitrate (NO) are two critical constituents of fine particulate matter (PM), significantly impacting air quality and public health. This study provides a comprehensive assessment of spatial and temporal variations in SO and NO levels across China from 1990 to 2020 by using a revised Community Multiscale Air Quality Modeling System (CMAQ), focusing on elucidating insights into source contributions and formation pathways. The results reveal that NO pollution has become increasingly significant, with its concentrations surpassing those of SO in most regions of China since 2017.

Hypoxia-Activated Liposomes Enable Synergistic Photodynamic Therapy for Oral Cancer.

Oral cancer is a significant public health problem, which is one of the most common malignancy with high mortality in the world. Apart from surgery, photodynamic therapy (PDT) is perceived as another capable method for the treatment of oral cancer. However, limited oxygen content of tumor microenvironment decreased the efficiency of current PDT.

Transcriptome Analysis Reveals Sugar and Hormone Signaling Pathways Mediating Flower Induction in Pitaya ().

Flower induction in pitaya () is regulated by complex gene networks involving multiple signaling pathways that ensure flower bud (FB) formation, but its molecular determinants remain largely unknown. In this study, we aimed to identify key genes and pathways involved in pitaya flower induction by analyzing transcriptomics profiles from differentiating buds. Our results indicate that the flower induction process is driven by a combination of sugar, hormone, transcription factor (TF), and flowering-related genes.

Transcription factors HmeWRKY33 and HmeWRKY51 regulate the susceptibility of pitaya to canker disease.

Pitaya canker disease, caused by , is the primary threat to pitaya cultivation, significantly compromising fruit quality and reducing yield. WRKY transcription factors are essential regulators in plant pathogen recognition and defense mechanisms, yet their specific roles in the development of pitaya canker disease remain largely unexplored. In this study, five genes (, , , , and ) associated with pitaya canker disease were identified through RNA-Seq analysis.

Multiple gRNAs-assisted CRISPR/Cas12a-based portable aptasensor enabling glucometer readout for amplification-free and quantitative detection of malathion.

Background: The threat of toxic malathion residues to human health has always been a serious food safety issue. The CRISPR/Cas system represents an innovative detection technology for pesticide residues, but its application to malathion detection has not been reported yet. In addition, the multiple-guide RNA (gRNA) powered-CRISPR/Cas biosensor has the advantages of being fast, sensitive and does not require pre-amplification.

Autophagy activation within inflammatory microenvironment improved the therapeutic effect of MSC-Derived extracellular Vesicle in SLE.

Introduction: Developing strategies to improve the therapeutic efficacy of mesenchymal stem cell (MSC)-derived extracellular vesicles (EVs) in autoimmune diseases have garnered increased attention.

Objectives: To evaluate whether rapamycin-induced autophagy within the systemic lupus erythematosus (SLE) inflammatory microenvironment (Rapa-SLE) augments the therapeutic effects of MSC-derived EVs in SLE.

Methods: The therapeutic potential of the resulting EVs (Rapa-SLE-EV) was assessed in MRL/lpr mice.