[Development and practice of an interactive chromatography learning tool for beginners based on GeoGebra： a case study of plate theory].

This study developed a GeoGebra platform-based interactive pedagogical tool focusing on plate theory to address challenges associated with abstract theory transmission， unidirectional knowledge delivery， and low student engagement in chromatography teaching in instrumental analysis courses. This study introduced an innovative methodology that encompasses theoretical model reconstruction， tool development， and teaching-chain integration that addresses the limitations of existing teaching tools， including the complex operation of professional software， restricted accessibility to web-based tools， and insufficient parameter-adjustment flexibility. An improved mathematical plate-theory model was established by incorporating mobile-phase flow rate， dead time， and phase ratio parameters.

The effects of remimazolam on the incidence of severe hypoxemia during sedated hysteroscopy for assisted reproduction in overweight or obese patients: a randomized controlled clinical trial.

Background: Hypoxemia is the most common adverse reaction during sedated hysteroscopy. Obesity is a risk factor for hypoxemia. For hysteroscopy, propofol is the most commonly used sedative.

GluA2 palmitoylation by SELENOK modulates AMPAR assembly and synaptic plasticity in Alzheimer's disease.

Se is essential for central nervous system function, and its deficiency is strongly associated with cognitive decline, especially in neurodegenerative disorders such as Alzheimer's disease (AD). Although Se exerts its effects through selenoproteins, the molecular basis of its neuroprotective action remains unclear. Among selenoproteins, the endoplasmic reticulum (ER)-resident selenoprotein K (SELENOK) is closely linked to cognitive function and therapeutic potential in AD.

A TPP-OH-based near-infrared fluorescent nanoprobe for cellular and in vivo HS detection and imaging.

The physiological and pathological processes of many diseases are influenced by hydrogen sulfide (HS), a physiologically active gaseous signaling molecule. Near-infrared fluorescent (NIR) probes are valued for their high specificity, sensitivity, simplicity, and safety. A near-infrared fluorescent probe (TPNPs), sensitive to HS, was designed and synthesized from 4-(10,15,20-triphenylporphyrin-5-yl)phenol (TPP-OH).

A Nanoplatform of Reversing Tumor Immunosuppressive Microenvironment Based on the NIR-II Gold Hollow Nanorod for the Treatment of Hepatocellular Carcinoma.

Advanced hepatocellular carcinoma (HCC) presents a strongly immunosuppressive tumor microenvironment, which enables tumor cells to evade immune cell attacks and hinder effective drug killing, thereby hindering the achievement of the desired therapeutic effect. In response, a novel nanoplatform- AuHNR@γ-FeO@Lenvatinib@β-Glucan (AFLG) with surface modified β-1,3-glucan is developed, which exhibits potent immunostimulatory effect and the capability of repolarizing macrophages, to counteract the immunosuppressive conditions present in the tumor microenvironment. Leveraging the hollow structure of gold nanorods, Lenvatinib is efficiently loaded, a first-line targeted drug for HCC, which effectively inhibits tumor angiogenesis.

Microneedles: multifunctional devices for drug delivery, body fluid extraction, and bio-sensing.

Microneedles represent a miniaturized mechanical structure with versatile applications, including transdermal drug delivery, vaccination, body-fluid extraction, and bio-sensing. Over the past two decades, microneedle-based devices have garnered considerable attention in the biomedicine field, exhibiting the potential for mitigating patient discomfort, enhancing treatment adherence, avoiding first-pass effects, and facilitating precise therapeutic interventions. As an application-oriented technology, the innovation of microneedles is generally carried out in response to a specific demand.

Associations between multimorbidity patterns and sarcopenia transitions in Chinese older adults.

Aim: Previous studies have shown that chronic diseases are strongly linked to the development of sarcopenia. Few studies have assessed the relationship between multimorbidity patterns and sarcopenia. This study aimed to investigate the impact of multimorbidity patterns on sarcopenia transitions in Chinese older adults.

Frailty detection with routine blood tests using data from the english longitudinal study of ageing (ELSA).

Purpose: Frailty is a rising global health issue in ageing society. Easily accessible and sensitive tools are needed for frailty monitoring while routine blood factors can be potential candidates.

Methods: Data from 1907 participants (aged 60 years or above) were collected from the 4th to 9th wave of the English longitudinal study of ageing.

The Evolution of Microfluidic-Based Drug-Loading Techniques for Cells and Their Derivatives.

Conventional drug delivery techniques face challenges related to targeting and adverse reactions. Recent years have witnessed significant advancements in nanoparticle-based drug carriers. Nevertheless, concerns persist regarding their safety and insufficient metabolism.

A stepwise multi-stage continuous dielectrophoresis separation microfluidic chip with microfilter structures.

The separation of target microparticles using microfluidic systems owns extensive applications in biomedical, chemical, and materials science fields. Integration of microfluidic sorting systems employing dielectrophoresis (DEP) technology has been widely investigated. However, enhancing separation efficiency, purity, stability, and integration remains a pressing issue.

Time and Space Dual-Blockade Strategy for Highly Invasive Nature of Triple-Negative Breast Cancer in Enhanced Sonodynamic Therapy Based on Fe-MOF Nanoplatforms.

Triple-negative breast cancer (TNBC), due to its high malignant degree and strong invasion ability, leads to poor prognosis and easy recurrence, so effectively curbing the invasion of TNBC is the key to obtaining the ideal therapeutic effect. Herein, a therapeutic strategy is developed that curbs high invasions of TNBC by inhibiting cell physiological activity and disrupting tumor cell structural function to achieve the time and space dual-blockade. The time blockade is caused by the breakthrough of the tumor-reducing blockade based on the ferroptosis process and the oxidation-toxic free radicals generated by enhanced sonodynamic therapy (SDT).

SELENOK-dependent CD36 palmitoylation regulates microglial functions and Aβ phagocytosis.

Amyloid-beta (Aβ) is a key factor in the onset and progression of Alzheimer's disease (AD). Selenium (Se) compounds show promise in AD treatment. Here, we revealed that selenoprotein K (SELENOK), a selenoprotein involved in immune regulation and potentially related to AD pathology, plays a critical role in microglial immune response, migration, and phagocytosis.

A Fully Elastic Wearable Electrochemical Sweat Detection System of Tree-Bionic Microfluidic Structure for Real-Time Monitoring.

Fresh sweat contains a diverse range of physiological indicators that can effectively reflect changes in the body. However, existing wearable sweat detection systems face challenges in efficiently collecting and detecting fresh sweat in real-time. Additionally, they often lack the necessary deformation capabilities, resulting in discomfort for the wearer.

Thermoelectric response of Janus monolayer M2P2S3Se3 (M = Zn and Cd).

Thermoelectric transport properties of Janus monolayers M2P2S3Se3 (M = Zn and Cd) are investigated by the first-principles based transport theory. The Zn2P2S3Se3 and Cd2P2S3Se3 monolayers are indirect-gap semiconductors. The high polarizability of M-Se/S bonds in the MS3Se3 distorted octahedrons leads to anharmonic phonon behavior, which produces an intrinsic lattice thermal conductivity (κl) as low as 1.

Thermoelectric response of single quintuple layer sodium copper chalcogenides persisting at high temperature.

The thermoelectric transport properties of two-dimensional (2D) layered NaCuX (X = S, Se) are investigated by employing first-principles based Boltzmann transport theory. Single quintuple NaCuX layers have a relatively large Seebeck coefficient (), electrical conductivity () and hence power factor (PF = ) for a p-type heavy doped region due to the valence band degeneracy. The largely reduced by dominant polar scattering leads to a PF up to 0.

Performance of a PLK1-based immune risk model for prognosis and treatment response prediction in breast cancer.

Objective: Polo-like kinase 1 (PLK1), a serine/threonine-protein kinase, functions as a potent oncogene in the initiation and progression of tumor. The aim of this study is to assess potential correlations between PLK1 expression and immune infiltration in breast cancer (BRCA) and construct a PLK1-based immune risk model applicable for prognosis and treatment response prediction in BRCA.

Methods: We collected data on PLK1 gene expression in BRCA patients from The Cancer Genome Atlas (TCGA) database.

Knockdown of the SELENOK gene induces ferroptosis in cervical cancer cells.

Selenoprotein K (SELENOK) is one of the endoplasmic reticulum (ER) proteins that mainly functions in the regulation of ER stress, calcium flux, and antioxidant defense. Reactive oxygen species (ROS) is one of the key indicators of ferroptosis, and SELENOK inhibition could disrupt ROS balance, and consequently might cause ferroptosis. However, there are no previous studies about the mechanism of SELENOK in ferroptosis by regulating ROS.

Design, synthesis and anti-AD effects of dual inhibitor targeting glutaminyl cyclase/GSK-3β.

Alzheimer's disease (AD), multifactorial disease, is recognized as one of the most common forms of dementia, and the efficacy of anti-AD drugs is limited clinically. Up-regulated glutaminyl cyclase (QC) and glycogen synthase kinase-3β (GSK-3β) have been identified as two critical elements involved in AD recently. Here, a series of novel chemicals containing maleimide and imidazole motif were designed and synthesized as dual inhibitors targeting QC and GSK-3β.

Knockout Induces Synaptic Deficits and Cognitive Dysfunction by Influencing Brain Glucose Metabolism.

Selenium, a trace element associated with memory impairment and glucose metabolism, mainly exerts its function through selenoproteins. is a selenoprotein located in the endoplasmic reticulum (ER) lumen. Our study demonstrates for the first time that knockout decreases synaptic plasticity and causes memory impairment in 10-month-old mice.

From Conventional to Microfluidic: Progress in Extracellular Vesicle Separation and Individual Characterization.

Extracellular vesicles (EVs) are nanoscale membrane vesicles, which contain a wide variety of cargo such as proteins, miRNAs, and lipids. A growing body of evidence suggests that EVs are promising biomarkers for disease diagnosis and therapeutic strategies. Although the excellent clinical value, their use in personalized healthcare practice is not yet feasible due to their highly heterogeneous nature.

Topology optimization based deterministic lateral displacement array design for cell separation.

Circulating tumor cell (CTC) can be used to guide cancer theranostics. How to isolate efficiently CTCs from blood owns great clinical requirement. Deterministic lateral displacement (DLD) is a pillar-array-based effective passive microfluidic method to separate cells based on their sizes.

Correction: Recent advances in microfluidic-based electroporation techniques for cell membranes.

Correction for 'Recent advances in microfluidic-based electroporation techniques for cell membranes' by Fei Wang , Lab Chip, 2022, 22, 2624-2646, https://doi.org/10.1039/D2LC00122E.