Scalable and lightweight deep learning for efficient high accuracy single-molecule localization microscopy.

Deep learning has significantly improved the performance of single-molecule localization microscopy (SMLM), but many existing methods remain computationally intensive, limiting their applicability in high-throughput settings. To address these challenges, we present LiteLoc, a scalable analysis framework for high-throughput SMLM data analysis. LiteLoc employs a lightweight neural network architecture and integrates parallel processing across central processing unit (CPU) and graphics processing unit (GPU) resources to reduce latency and energy consumption without sacrificing localization accuracy.

Engineered EVs from 3D-Cultured MSCs for Synergistic Modulation of Inflammatory Microenvironment and Cartilage Regeneration in Osteoarthritis.

Osteoarthritis (OA) is a common and serious joint disease characterized by synovitis and articular cartilage degeneration. Effective nonsurgical treatments for OA are still lacking. In this study, we designed an injectable temperature-sensitive hydrogel system delivering engineered extracellular vesicles for the treatment of OA.

Visceral adipose tissue in the lesser omentum predicts lymphovascular invasion, perineural invasion and survival in gastric cancer.

Background: Visceral adipose tissue is associated with clinical outcomes in patients with cancer. This study aimed to investigate the relationship between preoperative visceral adipose tissue in the lesser omentum and clinical prognosis, as well as lymphovascular invasion (LVI) and perineural invasion (PNI), in patients with gastric cancer (GC).

Patients And Methods: A total of 943 GC patients who underwent radical surgery across three centers in China were included in the study.

Histopathological evaluation of the efficacy of carbon nanoparticle staining in detecting lymph node metastasis in colorectal cancer surgery.

The aim of this study is to conduct a retrospective analysis on the correlation between lymph node staining and lymph node metastasis following laparoscopic radical resection of colorectal cancer using a carbon nanoparticles tracer. Additionally, the study seeks to investigate the potential clinical implications of carbon nanoparticles (CNs) in the context of colorectal cancer surgery. Clinical data was collected from patients who underwent laparoscopic radical resection of colorectal cancer at a ward of the Department of General Surgery, Nanjing Drum Tower Hospital between January 2022 and December 2023.

MARCH1, transcriptionally regulated by POU2F2, facilitates acute myeloid leukemia progression via inducing MYCT1 degradation.

Acute myeloid leukemia (AML) is a heterogeneous clonal disease. Membrane-associated ring-CH type finger 1 (MARCH1), a membrane-anchored E3 ubiquitin ligase, is highly expressed in AML. However, its role in AML remains unclear.

Evaluating the contribution of residential coal combustion to atmospheric nitrous acid (HONO) in winter.

Nitrous acid (HONO) is an important precursor of hydroxy radicals (OH) that determine atmospheric oxidation capacity and thus influence air quality and climate. Like vehicle exhausts and biomass burning, coal combustion is also recognized as a potential direct source of HONO. However, the quantification of HONO emissions from coal combustion is currently limited.

Dark-based optical sectioning assists background removal in fluorescence microscopy.

In fluorescence microscopy, a persistent challenge is the defocused background that obscures cellular details and introduces artifacts. Here, we introduce Dark sectioning, a method inspired by natural image dehazing for removing backgrounds that leverages dark channel prior and dual frequency separation to provide single-frame optical sectioning. Unlike denoising or deconvolution, Dark sectioning specifically targets and removes out-of-focus backgrounds, stably improving the signal-to-background ratio by nearly 10 dB and structural similarity index measure of images by approximately tenfold.

Development of a novel dual-emission probe for accurate detection and imaging of HClO in environmental and biological systems.

Hypochlorous acid (HClO) plays a crucial role in public and personal hygiene. However, the residual presence of HClO in water may pose potential risks to human health. Concurrently, HClO is indispensable in biological systems.

Solvent-Driven Enrichment and Multiplex Analysis of Local Anesthetics by Thin-Layer Chromatography Coupled with Surface-Enhanced Raman Spectroscopy.

Local anesthetics have been widely used in clinical analgesia due to their ability to provide effective regional pain management. Accurate measurement of local anesthetics in body fluids is crucial for ensuring patient medication safety and optimizing therapeutic efficacy. Herein, we present a convenient, economical, sensitive, and efficient TLC-SERS method for multiplex determination of six kinds of anesthetics (pro) in human plasma, including procaine hydrochloride (Pro), tetracaine hydrochloride (Tet), dibucaine (Dib), mepivacaine hydrochloride (Mep), lidocaine hydrochloride (Lid), and ropivacaine hydrochloride (Rop).

Self-Sustained Liquid Crystal Elastomer Actuators with Geometric Zero-Elastic-Energy Modes.

Recently, a novel and fascinating actuation mode of liquid crystal elastomers (LCEs), known as geometric zero-elastic-energy modes (ZEEMs), has drawn intensive research interest. Based on this actuation mechanism, LCE actuators exhibit untethered, autonomous movements under external stimulations, demonstrating significant potential for applications in intelligent soft robots, autonomous energy conversion systems, and smart optical tuning components. This perspective provides a timely summary of the current research on LCE actuators based on ZEEMs and highlights their future development trends and prospects, which will be of great interest to broad communities of researchers in fields of LCEs, biomimetic smart materials, soft robotics, and actuators.

Gene Mutation Characteristics and Prognostic Significance in Acute Myeloid Leukemia Patients From Northeast China.

A great part of studies on the correlation between gene mutations and prognosis in acute myeloid leukemia (AML) patients are based on Western populations. To profile the genomic landscape of AML patients in Northeast China, we retrospectively analyzed the clinical data of 377 newly diagnosed AML patients in Shengjing Hospital of China Medical University from 2016 to 2022 and compared them with data from other populations with different genetic backgrounds. The mutation status of , , , (CCAT enhancer binding protein alpha), , , , (DNA methyltransferase 3A), , , (enhancer of zeste 2), , , , and was acquired by next-generation sequencing (NGS) technology; meanwhile, the clinical data of the patients were collected.

Expression Patterns of Immune Checkpoint Molecules and Their Clinical Values in Gastric Neuroendocrine Neoplasms.

Introduction: Gastric neuroendocrine neoplasms (g-NENs) are a rare type of stomach tumor. However, limited data exist about the expression and clinical significance of B7 family ligands/receptors in patients with g-NENs. Thus, we conducted this study to address this issue in a cohort of 112 patients with g-NENs.

Characteristics and sex differences in bronchopulmonary dysplasia-related pulmonary hypertension.

Background: Pulmonary hypertension (PH) secondary to bronchopulmonary dysplasia (BPD) is associated with increased mortality. This study aims to elucidate the risk factors for BPD-PH development and the long-term prognostic factors in pediatric BPD.

Methods: We analyzed 1082 BPD patients under the age of three.

Functional Landscape of hnRNPA3 in Disease Pathogenesis.

The heterogeneous nuclear ribonucleic acid protein family participates in various intracellular reactions, such as RNA splicing, transport, DNA repair, cellular signal transduction, and gene expression regulation, and is involved in various disease processes. As a late-discovered member, heterogeneous nuclear ribonucleoprotein A3 has received increasing attention, but its main physiological functions and exact mechanisms involved in disease processes have not yet reached a consensus. In this review, we summarize the function of heterogeneous nuclear ribonucleoprotein A3 and the literature on its role in neurodegenerative and metabolic diseases, as well as in various tumors, to explore the applicability of heterogeneous nuclear ribonucleoprotein A3 as a therapeutic target and prognostic indicator.

Task Planning of Multiple Unmanned Aerial Vehicles Based on Minimum Cost and Maximum Flow.

With the rapid development of UAV technology, UAV delivery has gained attention for its potential to reduce labor costs. However, limitations in load capacity and energy restrict UAVs' distribution capabilities. This paper proposes a cooperative delivery scheme combining traditional trucks and UAVs to extend UAV coverage and improve delivery completion rates.

Dual-branch dynamic hierarchical U-Net with multi-layer space fusion attention for medical image segmentation.

Accurate segmentation of organs or lesions from medical images is essential for accurate disease diagnosis and organ morphometrics. Previously, most researchers mainly added feature extraction modules and simply aggregated the semantic features to U-Net network to improve the segmentation accuracy of medical images. However, these improved U-Net networks ignore the semantic differences of different organs in medical images and lack the fusion of high-level semantic features and low-level semantic features, which will lead to blurred or miss boundaries between similar organs and diseased areas.

Hybrid gabor attention convolution and transformer interaction network with hierarchical monitoring mechanism for liver and tumor segmentation.

Liver and tumor segmentation is an important technology for the diagnosis of hepatocellular carcinoma. However, most existing methods struggle to accurately delineate the boundaries of the liver and tumor due to significant differences in their shapes, sizes, and distributions, which leads to unclear segmentation of the liver contour and incorrect delineation of the lesion area. To address this gap, we propose a hybrid gabor attention convolution and transformer interaction network with hierarchical monitoring mechanism for liver and tumor segmentation, named HyborNet.

4D Printing of Shape-Morphing Liquid Crystal Elastomers.

In nature, biological systems can sense environmental changes and alter their performance parameters in real time to adapt to environmental changes. Inspired by these, scientists have developed a range of novel shape-morphing materials. Shape-morphing materials are a kind of "intelligent" materials that exhibit responses to external stimuli in a predetermined way and then display a preset function.

Injectable biomimetic hydrogel based on modified chitosan and silk fibroin with decellularized cartilage extracellular matrix for cartilage repair and regeneration.

Cartilage defect repair remains a challenge for clinicians due to the limited self-healing capabilities of cartilage. Microenvironment-specific biomimetic hydrogels have shown great potential in cartilage regeneration because of their excellent biological properties. In this study, a hydrogel system consisting of p-hydroxybenzene propanoic acid-modified chitosan (PC), silk fibroin (SF) and decellularized cartilage extracellular matrix (DCM) was prepared.

The diagnostic and therapeutic potential of multiple myeloma-associated circular RNAs.

Circular RNA (circRNA) was first discovered in viruses in 1974; they are primarily formed through back splicing, where a downstream splice donor is joined to an upstream splice acceptor, resulting in a closed circRNA transcript. Under normal conditions, most circRNAs are stably expressed; however, in pathological conditions, circRNAs can play critical roles in the disease process of multiple myeloma (MM) through mechanisms such as competing endogenous RNAs (ceRNAs), regulation of transcription and splicing, affecting protein expression and localization, and even direct encoding of peptides. In recent years, there has been increasing interest in the role of circRNAs in MM and their regulatory functions during the disease process.

Delineation of intersegmental plane: application of blood flow blocking method in pulmonary segmentectomy.

Background: The Modified Inflation-Deflation Method (MIDM) is widely used in China in pulmonary segmentectomies. We optimized the procedure, which was named as Blood Flow Blocking Method (BFBM), also known as "No-Waiting Segmentectomy". This method has produced commendable clinical outcomes in segmentectomies.

Water fraction of Schizochytrium sp. protein: A functional ingredient with superior gelation properties for sustainable food applications.

In order to assess the potential of fractionated Schizochytrium sp. protein as functional proteins, the proteins were fractionally extracted. The structure, thermal characteristic and cross-linking interaction of proteins, along with the gel properties of heat-induced gels were analyzed and compared to those of albumin from chicken egg white (ACEW).

An innovative BiOI@AgBiS@NaYF:Yb, Tm ternary heterostructure for efficient solar energy harvesting towards tetracycline hydrochloride degradation.

This study developed a BiOI@AgBiS@NaYF:Yb,Tm heterostructure photocatalyst. This design significantly enhances the generation and separation of charge carriers, leading to a marked improvement in solar energy harvesting. This advancement was effectively applied for the degradation of tetracycline hydrochloride.