Injectable Ca/Sr-crosslinked hydrogel with sustained release of LGK-974 for cartilage repair and osteoarthritis prevention via alleviating inflammatory microenvironment.

Cartilage injury would trigger a chronic inflammatory microenvironment that disrupts tissue regeneration and accelerates the onset of osteoarthritis (OA). However, current treatments primarily focus on the symptomatic relief of OA but fail to address the underlying pathophysiological issues. Herein, we developed an injectable Ca/Sr-crosslinked hydrogel incorporating LGK-974/polydopamine nanoparticles for immune regulation and cartilage regeneration.

Association between central obesity and the risk of glenohumeral joint osteoarthritis: a prospective study.

Background: The relationship between obesity and glenohumeral joint osteoarthritis (GJO) has long been a subject of doubt. Although body mass index (BMI) has been recognized as a risk factor by guidelines, it fails to reflect fat distribution, which may limit its clinical application value. Waist-to-height ratio (WHtR), as an indicator of central obesity, has shown good predictive performance in metabolic-related diseases.

Structural characterization of chelator-soluble and alkali-soluble pectins: Implications for juice quality.

Pectin is a critical structural component in fruit juices, influencing various juice quality attributes. The current study investigates the chelator-soluble pectin (CSP) and alkali-soluble pectin (ASP) extracted from different juices and their relation to juice quality. This study also explored the impact of high-pressure homogenization (HPH) on the structural characteristics of CSP and ASP.

Single-cell RNA sequencing uncovers dynamic roadmap during chicken spermatogenesis.

Background: Spermatogenesis is a pivotal biological process for the precise transmission of paternal genetic information, governed by a highly complex and dynamically regulated testicular microenvironment. Although mammalian research has characterized germ cell development at the single-cell level, differences in reproductive strategies limit the relevance of these findings to avian species.

Results: We employed single-cell RNA sequencing (scRNA-seq) to analyze the testes of the "Yufen 1" H line roosters at five distinct developmental stages: birth, rapid testicular development, sexual maturity, physical maturity, and senescence.

Optimization of a spiral photon sieve for vortex array generation using the hill climbing algorithm.

The generation of vortex arrays holds significant potential for applications in multi-channel fiber optics communication and optical micromanipulation due to their unique properties. These applications necessitate vortex array generation devices that are structurally simple and cost-effective. In this study, a composite spiral photon sieve (CSPS) is proposed, leveraging the traditional spiral photon sieve as the foundational structure and integrating the hill-climbing algorithm (HCA) for design optimization.

Assessing the adherence of large language models to clinical practice guidelines in Chinese medicine: a content analysis.

Objective: Whether large language models (LLMs) can effectively facilitate CM knowledge acquisition remains uncertain. This study aims to assess the adherence of LLMs to Clinical Practice Guidelines (CPGs) in CM.

Methods: This cross-sectional study randomly selected ten CPGs in CM and constructed 150 questions across three categories: medication based on differential diagnosis (MDD), specific prescription consultation (SPC), and CM theory analysis (CTA).

Ultratough, Processable Bioplastics Enabled by Triple Interlocking of Lignin and Cellulose.

Sustainable and biodegradable bioplastics from natural lignocellulose offer a promising alternative to petroleum-based plastics, yet they often exhibit limited toughness and processability due to the inherent rigidity of polymer segments. Herein, we have developed a triple interlocking strategy to fabricate a high-strength, ultratough, and processable Bioplastic (denoted as CEL Bioplastic) from cellulose and lignin in the pulp/paper industry. In this process, we leverage room-temperature esterification of long-chain fatty acids with cellulose and lignin to produce a fully biobased CEL Bioplastic, distinguished by a robust triple-interlocking architecture that combines robust physical chain entanglements, cross-linked ester bonds, and densely packed hydrogen bonds.

The Nature of Oxidized Oxygen in Oxygen Redox Cathodes.

Oxygen redox cathodes (ORCs) are expected to surpass the capacity limits of conventional batteries. However, the commercialization of ORCs has suffered from severe voltage decay due to irreversible structural reconstructions, which are related to the evolution of oxidized oxygen upon charging. Several models have been proposed to elucidate the relationship between oxygen redox and cathode failures.

P4HA2 promotes the progression of thyroid cancer by regulating PFKP-mediated glycolysis.

Emerging evidence suggests that prolyl-4-hydroxylase α subunit 2 (P4HA2) plays critical roles in cancer progression through multiple mechanisms. Notably, P4HA2 has been implicated in modulating glycolytic pathways in malignancies. Phosphofructokinase (PFKP), a key glycolytic enzyme, exhibits significant overexpression in thyroid cancer.

Exploring the incidence rate and imaging differential diagnosis of anterior mediastinal lesions: an 11-year retrospective study based on 2,626 cases.

Background: There are many complex types of anterior mediastinal lesions, and preoperative differential diagnosis is difficult in clinical practice. This study aimed to explore the incidence of different anterior mediastinal lesions and their differential diagnosis based on clinical and imaging features.

Methods: We examined the local incidence of anterior mediastinal lesions and their different types in 2,626 patients with anterior mediastinal lesions.

PEAK1 maintains tight junctions in intestinal epithelial cells and resists colitis by inhibiting autophagy-mediated ZO-1 degradation.

Tight junctions are crucial for maintaining intestinal barrier homeostasis, but how organisms modulate these junctions remain unclear. Here, we show a role for PEAK1 at cell-cell contact sites, where it interacts with ZO-1 via a conserved region spanning amino acids 714-731. This interaction masks the LC3-interacting region on ZO-1, preventing autophagy-mediated ZO-1 degradation and preserving the integrity of tight junctions in intestinal epithelial cells.

Single-cell transcriptomics identifies PDGFRA progenitors orchestrating angiogenesis and periodontal tissue regeneration.

Periodontal bone defects, primarily caused by periodontitis, are highly prevalent in clinical settings and manifest as bone fenestration, dehiscence, or attachment loss, presenting a significant challenge to oral health. In regenerative medicine, harnessing developmental principles for tissue repair offers promising therapeutic potential. Of particular interest is the condensation of progenitor cells, an essential event in organogenesis that has inspired clinically effective cell aggregation approaches in dental regeneration.

Characterization of pectic polysaccharides and apple juice properties for 13 apple varieties.

The physicochemical properties of apple juice from 13 apple varieties were correlated with the structural features of isolated apple pectin populations. Mathematical models explaining these structural properties, revealed variations in pectins as present in alcohol insoluble solids (AIS), water-soluble solids (WSS), and chelating soluble solids (ChSS). Significant differences in turbidity (89.

Functional analysis of Mu-class glutathione S-transferase HdGSTm1 from Haemaphysalis doenitzi with a focus on the roles in the tick blood sucking.

During blood feeding, ticks face significant oxidative stress due to hemoglobin breakdown. In this context, GSTs are proposed to act as critical antioxidants, possibly by detoxifying lipid peroxides. The transcriptome of H.

Development and validation of growth prediction models for multiple pulmonary ground-glass nodules based on CT features, radiomics, and deep learning.

Background: The development of growth prediction models for multiple pulmonary ground-glass nodules (GGNs) could help predict their growth patterns and facilitate more precise identification of nodules that require close monitoring or early intervention. Previous studies have demonstrated the indolent growth pattern of GGNs and developed growth prediction models; however, these investigations predominantly focused on solitary GGN. This study aimed to investigate the natural history of multiple pulmonary GGNs and develop and validate growth prediction models based on computed tomography (CT) features, radiomics, and deep learning (DL) as well as compare their predictive performances.

Suberin in plants: biosynthesis, regulation, and its role in salt stress resistance.

Soil salinization represents a significant global ecological challenge. Plants encounter salt stress in their growth environments. Suberin, a hydrophobic polymer, plays a critical role in plant salt tolerance.

Association between DASH diet and metabolic syndrome in US adults: a cross-sectional study.

Objective: Metabolic syndrome (MetS) is a global issue increasingly threatening human health and the quality of life. This study aimed to explore the relationship between the Dietary Approaches to Stop Hypertension (DASH) diet and MetS, with the goal of providing insights into how dietary interventions may be associated with MetS.

Methods: We utilized data from the National Health and Nutrition Examination Survey 2005-2008 and divided DASH scores into five quintile groups.

Development of intelligent tools to predict neuroblastoma risk stratification and overall prognosis based on multiphase enhanced CT and clinical features.

Background: Neuroblastoma (NB) is a common malignancy in children, and accurate risk stratification and prognostic assessment are essential for personalized treatment. Current tumor assessment methods rely on clinical features and conventional imaging techniques, which have limited predictive accuracy. The aim of this study was to develop a deep learning model based on multiphase enhanced CT images and clinical features to improve the accuracy of risk stratification and prognostic assessment of NB.

Investigation of Novel Strategies to Improve the Storage Stability and the Antimicrobial Activity of Ozonized Sunflower Oil.

Ozonized sunflower oil has been found to have a valuable antimicrobial activity against bacteria, viruses, and fungi. However, delayed cutaneous wound healing, severe adverse skin reactions such as pain, burns, irritation, and unsatisfactory storage stability have limited its clinical use as it is known that the performance of ozonized sunflower oil is reflected by core indicators such as iodine value (IV), peroxide value (PV), and acid value (AV). Aiming to improve the above characteristics, ozone oxidation conditions such as the oil water content of raw materials, stirring speed, and ozonized time were successfully optimized, and the optimized conditions were 5%, 300 rpm, and 6 h, respectively.

Ultrasonic-assisted deep eutectic solvent extraction and identification of phenolic compounds from Atractylodes chinensis adventitious root culture.

Atractylodes chinensis is a traditional Chinese medicinal plant, which contains lots of volatile oils and phenolic compounds. However, the current researches mainly concentrate on its volatile oils, and only a few studies on phenolic compounds, especially on phenolic compounds extraction in adventitious roots of A. chinensis.

Experimental research on fungal inhibition using dissolving microneedles of terbinafine hydrochloride nanoemulsion for beta-1,3-glucanase.

: Onychomycosis is characterized by high transmission and low recovery rates, leading to a lack of optimal treatments. This study used dissolving microneedles as drug carriers to design dual-loaded DMN patches with β-1,3-glucanase and terbinafine hydrochloride nanoemulsion. : Dissolving microneedles of terbinafine hydrochloride nanoemulsion for beta-1,3-glucanase (Gls-TBH-NE-DMN) were prepared using the centrifugal molding method.

Large Language Model–Assisted Risk-of-Bias Assessment in Randomized Controlled Trials Using the Revised Risk-of-Bias Tool: Evaluation Study.

Background: The revised Risk-of-Bias tool (RoB2) overcomes the limitations of its predecessor but introduces new implementation challenges. Studies demonstrate low interrater reliability and substantial time requirements for RoB2 implementation. Large language models (LLMs) may assist in RoB2 implementation, although their effectiveness remains uncertain.

Overactive Bladder is Positively Associated With the Prevalence and Mortality of Cancer Among US Adults.

Background: Overactive bladder (OAB) is a prevalent urological condition often perceived as negligible or non-threatening, leading to its potential links with severe health conditions being overlooked. Notably, the relationship between OAB and cancer remains unexplored. This study endeavors to elucidate the association of OAB with cancer prevalence and mortality risks.

MARCH1, transcriptionally regulated by POU2F2, facilitates acute myeloid leukemia progression via inducing MYCT1 degradation.

Acute myeloid leukemia (AML) is a heterogeneous clonal disease. Membrane-associated ring-CH type finger 1 (MARCH1), a membrane-anchored E3 ubiquitin ligase, is highly expressed in AML. However, its role in AML remains unclear.

Whole-genome sequencing revealed genetic structure, patterns of selection and molecular identity card in "Yufen 1" D line chickens.

The "Yufen 1" D line (D) chicken is characterized by strong disease resistance and a high feed conversion rate as a newly bred line. There are few reports on D chicken. In order to investigate the genetic diversity, population structure and selection signals of the D chicken, this study analyzed the genome-wide data of 15 D line chickens and 11 publicly available Chinese indigenous chicken breeds, the red jungle fowls.