Managing Perforating Internal Root Resorption in Mature Incisor with Laser- Assisted Regenerative Endodontic Procedures.

Introduction: This report presented the successful application of laser-assisted regenerative endodontic procedures (REPs) in managing perforating internal root resorption (PIRR) in a mature incisor.

Methods: A 24-year-old female with a history of trauma was referred for treatment of her maxillary anterior tooth. Clinical examination and cone-beam computed tomography imaging revealed that tooth #9 had PIRR with a lateral inflammatory lesion and thin canal walls.

Suberin in plants: biosynthesis, regulation, and its role in salt stress resistance.

Soil salinization represents a significant global ecological challenge. Plants encounter salt stress in their growth environments. Suberin, a hydrophobic polymer, plays a critical role in plant salt tolerance.

Greenhouse gas emissions and carbon budget estimation in constructed wetlands treating aquaculture tailwater: Insight from seasonal dynamics of dissolved organic matter and microbial community.

Constructed wetlands (CWs) effectively treat aquaculture tailwater but face challenges from greenhouse gas (GHG) emissions affecting their carbon sink potential. Few systematic studies have been conducted to identify the drivers of temporal variation of GHG emissions in CWs. Through year-round monitoring of an integrated vertical flow constructed wetland (IVCW), we identified seasonal dynamics in carbon oxide (CO), methane (CH), and nitrous oxide (NO) emissions, with summer fluxes exceeding winter levels by 12-fold for CH and 3-fold for NO.

Distribution Pattern of Endangered Carruth. in China Under Climate-Change Scenarios Using the MaxEnt Model.

Understanding the potential distribution patterns and habitat suitability of threatened species under climate change scenarios is essential for conservation efforts. This study aimed to assess the current and future distribution patterns of the endangered in China using the MaxEnt model under two contrasting climate change scenarios: SSP1-2.6 (low emissions) and SSP3-7.

[Aquatic Community Structure and Biological Assessment of Water Quality in Urban Lakes of the Yangtze River Downstream： A Case Study of Three Lakes in Wuhu City].

The Yangtze River constitutes a crucial aquatic system in China, and lakes downstream play a significant role in the stability of ecosystem functions. However, human activities have caused the lakes to suffer from eutrophication. Moreover, it is important to investigate the aquatic ecosystems of Qingting Lake, Fengming Lake, and Yin Lake in Wuhu City, Anhui Province, China, for tracing and controlling eutrophication.

Use of virtual simulation for regenerative endodontic training: randomized controlled trial.

Objectives: This study aimed to develop an experimental teaching module based on virtual simulation (VS) to facilitate the instruction of undergraduates in regenerative endodontic procedures (REPs) and to assess its impact on academic outcomes and students' perceptions.

Methods: A REPs-VS platform with detailed diagnostic thinking, treatment protocol, and outcome evaluation scripts we developed. In total, 123 undergraduates in the fourth year were randomly divided into group A (REPs-VS teaching mode) and group B (traditional teaching mode).

Novel role of FTO in regulation of gut-brain communication via -produced hydrogen sulfide under arsenic exposure.

Fat mass and obesity-associated protein (FTO) is the key demethylase that reverses the abnormally altered N6-methyladenosine (m6A) modification in eukaryotic cells under environmental pollutants exposure. Arsenic is an environmental metalloid and can cause severe symptoms in human mainly through drinking water. However, there is no specific treatment for its toxic effects due to the uncovered mechanisms.

Evaluating the effectiveness of a novel training model in dental preclinical regenerative endodontic education.

Aim: Regenerative endodontic procedures (REPs) are tissue engineering-based treatments aimed at regenerating the pulp-dentin complex. However, these procedures are not widely practiced or well-known among dentists due to a lack of training. This study aims to develop a practical hands-on training model for REPs to enhance students' theoretical knowledge and practical skills.

LC3B-regulated autophagy mitigates zinc oxide nanoparticle-induced epithelial cell dysfunction and acute lung injury.

Zinc oxide nanoparticles (ZnONPs) are widely utilized across various industries, raising concerns about their potential toxicity, especially in the respiratory system. This study explores the role of autophagy, regulated by microtubule-associated protein 1A/1B-light chain 3B (LC3B), in ZnONPs-induced toxicity using both in vivo (LC3B knockout mice) and in vitro (BEAS-2B cells) models. Our findings demonstrate that LC3B-regulated autophagy mitigates ZnONPs-induced epithelial cell dysfunction and acute lung injury.

Definition, risk factors, and outcome analysis of prolonged mechanical ventilation in children.

Objective: This study aims to explore the time threshold for defining prolonged mechanical ventilation (PMV) in children, along with its risk factors and outcomes.

Methods: A prospective cohort study was conducted on children aged 29 days-18 years, who were consecutively admitted to two Pediatric Intensive Care Units (PICUs) at Children's Hospital of Chongqing Medical University, from October 2020 to June 2021. The study included patients receiving mechanical ventilation (MV) for more than 2 days (each day >6 h).

Exogenous auxin alters the polycyclic aromatic hydrocarbons apoplastic and symplastic uptake by wheat seedling roots.

Polycyclic aromatic hydrocarbons (PAHs) are a category of organic pollutants known for their high carcinogenicity. Our previous research has illustrated that plant roots actively absorb PAHs through a co-transport mechanism with H ions. Because auxin can increase the H-ATPase activity, the wheat roots were exposed to PAHs with/without auxins to study whether auxins facilitate the uptake of PAHs by plant roots and to gain insights into the underlying mechanisms of this process.

Mechanistic insights into auxin-enhancing polycyclic aromatic hydrocarbon uptake by wheat roots: Evidence from in situ intracellular pH and root-surface H flux.

Polycyclic aromatic hydrocarbons (PAHs) are a group of extremely carcinogenic organic pollutants. Our previous findings have demonstrated that plant roots actively take up PAHs through co-transport with H ions. Auxin serves as a pivotal regulator of plant growth and development.

High-Resolution Performance of Polycaprolactone Functionalized with Guanidinium Ionic Liquid for Gas Chromatography.

This work firstly reported a new polycaprolactone based material functionalized with guanidinium ionic liquid (PCL-GIL) as the stationary phase with high resolution performance for capillary gas chromatography (GC). It is composed of polycaprolactone (PCL) and guanidinium ionic liquid (GIL) with amphiphilic conformation. The PCL-GIL capillary column coated by static method exhibited high column efficiency of 3942 plates/m and moderate polarity.

An Amphiphilic Star-Shaped Polymer (Star-PEG-PCL2) used as a Stationary Phase for GC.

A star-shaped polymer (Star-PEG-PCL2) was synthesized with PCL and PEG and used as a stationary phase for gas chromatography. The statically coated Star-PEG-PCL2 column exhibited an efficiency of 2260 plates/m determined by naphthalene at 120 °C and moderate polarity. The Star-PEG-PCL2 column showed high resolution performance for isomers of a wide ranging polarity, including methylnaphthalenes, halogenated benzenes, nitrobenzene, phenols, and anilines, and displayed dual-nature selectivity for a mixture of 17 analytes.

Efficient gas chromatographic separation of xylene and other aromatic isomers by using pillar[6]arene-based stationary phase.

The separation of aromatic isomers, in particular xylene isomers, represents a big issue in chemical and petroleum industries, owing to their similar molecular sizes and boiling points. In this work, the investigation ofpillar[6]arene derivative modified by long alkyl chains (P6A-C10) as a stationary phase for high-resolution gas chromatographic (GC) separations of xylene isomers is presented. Pillar[n]arenes are a new class of macrocyclic hosts that can accommodate specific guests due to their highly symmetrical and rigid pillar architectures with π-electron rich cavities.

Separation performance of the calix[8]arene functionalized with polyethylene glycol units for capillary gas chromatography.

In this paper, an amphiphilic calix[8]arene with polyethylene glycol unit branches (C8A-PEG) was synthesized and applied for capillary gas chromatography (GC). The C8A-PEG was coated on the inner wall of a capillary column by a static method with the column efficiency of 3165 plates/m and polar nature. As demonstrated, the C8A-PEG column has excellent physicochemical properties and separation performance since it has π-electron-rich 3D cavity which combines with polar PEG units.

Evaluation of transcriptomic molecular classification, biological behavior, and clinicopathological features in hepatocellular carcinoma.

Background: Studies in France, Korea, and Singapore found that G1-G6 transcriptomes are involved in hepatocellular carcinoma (HCC) carcinogenesis. However, the suitability of this method in Chinese HCC patients has remained unknown.

Methods: The correlation between the G1-G6 molecular classification and clinicopathological features were analyzed in 107 Chinese HCC patients through the retrospective cohort study.

Chromatographic Separation of Aromatic Amine Isomers: A Solved Issue by a New Amphiphilic Pillar[6]arene Stationary Phase.

In this work, the fabrication, synthesis, and characterization of a new stationary phase based on an amphiphilic pillar[6]arene (P6A-C10-2NH) for gas chromatographic analyses are reported. The gas chromatography (GC) column prepared with P6A-C10-2NH stationary phase exhibited a medium polarity, an efficiency of 3219 plates/m, and unmatched resolving capabilities toward chloroaniline, bromoaniline, iodoaniline, and toluidine isomers. Furthermore, the P6A-C10-2NH column showed excellent repeatability with maximum relative standard deviations equal to 0.

A New Capillary Gas Chromatography Column Based on Poly(ethylene glycol) Methyl Ether-Functionalized Calix[4]arene.

In this work, a novel capillary column (C4A-mPEG) with a calixarene-based polymer stationary phase (poly(ethylene glycol) methyl ether-functionalized 4-tert-butylcalix[4]arene) was designed and used for gas chromatographic (GC) separations. The C4A-mPEG capillary column, prepared by the static coating method, showed moderate polarity and a column efficiency of 2332 plates/m, determined by 1-octanol at 120 °C. The separation features of C4A-mPEG stationary phase, resulting from its unique structure and multiple molecular recognition processes with analytes, including π-π, H-bonding, dipole-dipole, and van der Waals interactions, allowed to obtain high-resolution performances for a wide range of compounds and their isomers, especially benzaldehydes, phenols, and anilines.

Solar-induced chlorophyll fluorescence imperfectly tracks the temperature response of photosynthesis in winter wheat.

Solar-induced fluorescence (SIF) is a promising proxy for photosynthesis, but it is unclear whether it performs well in tracking the gross primary productivity (GPP) under different environmental conditions. In this study, we investigated the dynamics of the two parameters from October 2020 to June 2021 in field-grown winter wheat (Triticum aestivum) and found that the ability of SIF to track GPP was weakened at low temperatures. Accounting for the coupling of light and temperature at a seasonal scale, we found that SIF yield showed a lower temperature sensitivity and had a lower but broader optimal temperature range compared with light-use efficiency (LUE), although both SIF yield and LUE decreased in low-temperature conditions.

BTLACD200 B cells dictate the divergent immune landscape and immunotherapeutic resistance in metastatic vs. primary pancreatic cancer.

Response to cancer immunotherapy in primary versus metastatic disease has not been well-studied. We found primary pancreatic ductal adenocarcinoma (PDA) is responsive to diverse immunotherapies whereas liver metastases are resistant. We discovered divergent immune landscapes in each compartment.

Mechanistic insights into phenanthrene acropetal translocation via wheat xylem: Separation and identification of transfer proteins.

Polycyclic aromatic hydrocarbons (PAHs) have the potential to cause cancer, teratogenicity, and mutagenesis in humans. Long-term plant safe production relies on how PAHs are transported and coordinated across organs. However, the acropetal transfer mechanism of PAHs in staple crop stems, particularly in xylem, a critical path, is unknown.

Cellular Process of Polystyrene Nanoparticles Entry into Wheat Roots.

Nanoscale plastic particles are widely found in the terrestrial environment and being increasingly studied in recent years. However, the knowledge of their translocation and accumulation mechanism controlled by nanoplastic characterizations in plant tissues is limited, especially in plant cells. Here, 20 mg L polystyrene nanoparticles (PS NPs) with different sizes and amino/carboxy groups were employed to investigate the internalization process in wheat roots and cells.