Single cell RNA seq reveals the pro-regenerative phenotype of thrombospondin-2 deficient dermal fibroblasts.

Fibroblasts display complex functions linked to distinct gene expression profiles. These profiles influence matrix production, cell communication, and tissue development and repair. Thrombospondin-2 (TSP2), produced by fibroblasts, is a potent angiogenesis inhibitor and negatively associated with tissue repair.

A simple VA-ECMO bundle in adult patients with cardiogenic shock: an analysis of ELSO registry.

Background: The optimal management strategy for patients undergoing veno-arterial extracorporeal membrane oxygenation (VA-ECMO) for cardiogenic shock (CS) remains uncertain. To evaluate the impact of "bundled" physiologic targets on outcomes in VA-ECMO patients.

Methods: This retrospective cohort study analyzed data from the Extracorporeal Life Support Organization (ELSO) registry, including adult patients receiving VA-ECMO for CS between 2013 and 2022.

Geometrically insensitive deform-and-go liquid crystal elastomer actuators through controlled radical diffusion.

The geometric shape and programming of mesogen alignment are two critical prerequisites for the effective actuation of liquid crystal elastomer (LCE) actuators. However, existing alignment programming approaches inevitably impose limitations on the geometric design of LCEs. In this study, we introduce a controlled radical diffusion mechanism that enables geometrically insensitive programming of actuation.

Spherical Indentation Behavior of DD6 Single-Crystal Nickel-Based Superalloy via Crystal Plasticity Finite Element Simulation.

Nickel-based superalloys are widely utilized in critical hot-end components, such as aeroengine turbine blades, owing to their exceptional high-temperature strength, creep resistance, and oxidation resistance. During service, these components are frequently subjected to complex localized loading, leading to non-uniform plastic deformation and microstructure evolution within the material. Combining nanoindentation experiments with the crystal plasticity finite element method (CPFEM), this study systematically investigates the effects of loading rate and crystal orientation on the elastoplastic deformation of DD6 alloy under spherical indenter loading.

EPS8L2 drives colorectal cancer cell proliferation and migration via YBX1-dependent activation of G3BP2 transcription.

Colorectal cancer (CRC) remains a leading cause of cancer-related mortality worldwide, characterized by molecular heterogeneity and limited therapeutic options. Here, we identified EPS8L2 as a novel driver of colorectal tumorigenesis. EPS8L2 is significantly upregulated in CRC tissues and negatively correlated with patients' prognosis.

Report on the adherence to key management strategies and mortality rates in patients with acute myocardial infarction-related cardiogenic shock: Findings from the Improving Care for Cardiovascular Disease in China-Acute Coronary Syndrome project.

Background: Acute myocardial infarction-related cardiogenic shock (AMI-CS) is a severe complication with high in-hospital mortality. However, real-world data on AMI-CS care are limited. This study aimed to evaluate the adherence to management strategies for AMI-CS.

The Effect of the Antidepressant Citalopram on the Bioconcentration and Biomarker Response of at Environmentally Relevant Concentrations.

The widespread use and pseudo-persistent occurrence of the antidepressant citalopram (CIT) could pose a potential ecological risk in the aquatic environment. The message about the bioconcentration and sensitive biomarker identification of CIT at the environmentally relevant concentrations is limited. In this study, an integral evaluation of the phenotypic and biochemical effects of CIT on () was conducted at 0.

Expert Consensus on Clinical Application of Implantable Biventricular Assist Devices.

While biventricular assist devices (BiVADs) remain underutilized in Western countries for biventricular heart failure (BHF), their application is expanding in China. This consensus synthesizes international guidelines, medical evidence, and Chinese clinical expertise to establish standardized protocols for BiVAD management. Key recommendations include: (1) Preoperative right heart catheterization and echocardiography for central venous pressure (CVP): pulmonary capillary wedge pressure (PCWP) ratio and pulmonary artery pulsatility index (PAPi) assessment (Class I); (2) BiVAD indication in refractory BHF or high-risk right heart failure post-left ventricular assist device (LVAD) implantation (Class IIa); (3) Right atrial implantation as the preferred surgical approach (Class IIa); (4) Warfarin-based anticoagulation (INR 2.

Greenhouse gas emissions and carbon budget estimation in constructed wetlands treating aquaculture tailwater: Insight from seasonal dynamics of dissolved organic matter and microbial community.

Constructed wetlands (CWs) effectively treat aquaculture tailwater but face challenges from greenhouse gas (GHG) emissions affecting their carbon sink potential. Few systematic studies have been conducted to identify the drivers of temporal variation of GHG emissions in CWs. Through year-round monitoring of an integrated vertical flow constructed wetland (IVCW), we identified seasonal dynamics in carbon oxide (CO), methane (CH), and nitrous oxide (NO) emissions, with summer fluxes exceeding winter levels by 12-fold for CH and 3-fold for NO.

Supramolecular hydrogel actuators with reprogrammable magnetic orientation by locally mediated viscoelasticity and pinning force.

Magnetic soft actuators with reprogrammable deformations have gained substantial attention due to their adaptability for various applications. However, achieving precise and local reorientation of magnetic particles remains challenging. Here, we present a strategy to locally tailor the viscoelasticity of magnetic supramolecular hydrogels, facilitate reorientation of the embedded magnetic particles, and enable reprogrammable magnetoactuated deformation and locomotion of the composite gels.

Selective enhancement of the interneuron network and gamma-band power via GluN2C/GluN2D NMDA receptor potentiation.

N-Methyl-d-aspartate receptors (NMDARs) are a family of ligand-gated ionotropic glutamate receptors that mediate a slow, calcium-permeable component to excitatory neurotransmission. The GluN2D subunit is enriched in GABAergic inhibitory interneurons in cortical tissue. Diminished levels of GABAergic inhibition contribute to multiple neuropsychiatric conditions, suggesting that enhancing inhibition might have therapeutic utility, thus making GluN2D modulation an attractive drug target.

Comparative Toxicokinetics and Biomarker Responses of Typical Psychiatric Pharmaceuticals in .

The widespread availability and pseudo-persistence of typical psychiatric pharmaceuticals (PDs) can have serious impacts on aquatic ecosystems and even human health. However, the toxicokinetics of typical PDs and the corresponding enzymatic biomarker responses are unclear. In this study, eight typical PDs [carbamazepine (CBZ), citalopram (CIT), sertraline (SER), venlafaxine (VLF), amitriptyline (AMT), chlorpromazine (CPM), quetiapine (QTP) and clozapine (CLZ)] were selected to study the uptake, depuration and biological effects of PDs in .

Preliminary research indicates that mechanical force through Pioze1 enhances local immunity during NPWT treatment for spinal infections.

Objective: This study aims to investigate the mechanism by which Negative Pressure Wound Therapy (NPWT) regulates local immune responses in spinal infection through Piezo1-mediated mechanical stress, and elucidate its potential role in the treatment of spinal infections.

Methods: From July 2021 to April 2022, a total of 7 patients with spinal infection treated with NPWT at our department were included in the study. The study analyzed clinical outcomes of spinal infection surgeries, including operative duration, intraoperative blood loss, postoperative drainage, improvements in pain levels as measured by the Visual Analogue Scale (VAS), and inflammatory markers such as C-reactive protein (CRP) and Erythrocyte Sedimentation Rate (ESR) measured one week before and after the procedure.

The Association of Lipoprotein(a) with Major Adverse Cardiovascular Events after Acute Myocardial Infarction: A Meta-Analysis of Cohort Studies.

Background: Despite evidence suggesting a link between lipoprotein(a) (Lp(a)) and the occurrence of acute myocardial infarction (AMI), the relationship regarding prognoses related to AMI remains unclear. This meta-analysis was conducted to summarize the association between Lp(a) and the risks of major adverse cardiovascular events (MACEs) among populations surviving AMI.

Methods: We searched PubMed, Embase, Web of Science, MEDLINE, and Cochrane Library databases until February 14, 2024.

Analysis of the feasibility of a low-anticoagulation strategy in patients undergoing post-cardiotomy extracorporeal membrane oxygenation: a retrospective cohort study.

Background: Extracorporeal membrane oxygenation (ECMO) is increasingly used in patients after cardiac surgery; however, anticoagulation management has consistently been challenging. This study aimed to explore the feasibility of a low-anticoagulation strategy for post-cardiotomy ECMO (PC-ECMO).

Methods: A retrospective comparison was performed between two anticoagulation targets in adult patients undergoing veno-arterial ECMO after cardiac surgery at the Beijing Anzhen Hospital (Beijing, China) between January 2018 and November 2023.

Using a Cardiogenic Shock Classification System for Predicting Postcardiotomy Shock Mortality.

Background: Cardiogenic shock (CS) is a life-threatening hemodynamic state. Patients with differing shock severity show varying responsiveness to clinical interventions. CS also occurs in patients who have undergone cardiac surgery.

Diluted alkaline pretreatment in hexafluoroisopropanol facilitates chemoenzymatic depolymerization of polyethylene terephthalate.

Enzymatic PET degradation presents a sustainable and eco-friendly solution for recycling and upgrading PET materials. While various PET-degrading enzymes have proven effective in converting low-crystallinity PET into monomers, their efficiency decreases significantly for high-crystallinity PET. Given that most commercially available PET products are highly crystalline and have a limited specific surface area, conventional methods typically resort to heat treatment and ball milling to achieve decrystallization and micronization before enzymatic hydrolysis.

Risk factors for surgical site infections after orthopaedic surgery: A meta-analysis and systematic review.

The objective of this meta-analysis was to investigate the occurrence and determinants of surgical site infections (SSIs) in individuals following orthopaedic surgical procedures. A systematic exploration of articles concerning factors predisposing individuals to SSIs after orthopaedic surgery was conducted across multiple databases, including PubMed, Embase, Cochrane Library and Web of Science, up to March 20, 2024. The Stata 15.

A radiomics nomogram based on MRI for differentiating vertebral osteomyelitis from vertebral compression fractures.

Objectives: This study aims to investigate the value of a radiomics nomogram based on magnetic resonance imaging (MRI) in distinguishing vertebral compression fractures (VCFs) from vertebral osteomyelitis (VOs).

Materials And Methods: We conducted a retrospective analysis of the clinical data from 100 patients with VCFs and VOs, respectively at our hospital. The cases were randomly divided into training (n = 140) and testing sets (n = 60) in a 7:3 ratio.

Superlarge depth range 3D measurement based on dual-focal optimization strategy.

Fringe projection profilometry (FPP) is a widely applied high accuracy three-dimensional measurement technique, and the binary defocusing technique is commonly used to improve measurement speed. However, the measurement accuracy is affected by the defocusing region. In the traditional binary defocusing system, only the front section or the back section of proper defocusing region can be used as the measurement depth range.

Report on the clinical outcomes of using postural drainage with intervertebral space as the main focus for managing thoracolumbar tuberculosis: Eight case reports.

Rationale: Conservative treatment has shown limited effectiveness in managing thoracolumbar tuberculosis (TB) that extends to the intervertebral space, as antibiotics are unable to penetrate avascular intervertebral discs, while conventional surgery is known for its extensive trauma and slow healing process.

Patient Concerns: Infection of the thoracic and lumbar vertebrae with tuberculosis can lead to difficulties in treatment due to involvement of the intervertebral space.

Diagnosis: The diagnosis of TB was confirmed through clinical manifestations, impact studies, and T-spot experiments.

Overexpression of miR-99a promoted expansion and suppressed differentiation of hematopoietic stem/progenitor cells.

MicroRNAs (miRNAs) are a class of non-coding RNAs involved in a variety of pathophysiological processes. We have previously reported that the abnormally high expression of miR-99a is associated with drug resistance and poor prognosis in acute myeloid leukemia. However, the impact of miR-99a on normal hematopoiesis is not well understood.

Triglyceride-glucose index correlates with the incidences and prognoses of cardiac arrest following acute myocardial infarction: data from two large-scale cohorts.

Background: The triglyceride-glucose (TyG) index, renowned for its efficacy and convenience in assessing insulin resistance, has been validated as a reliable indicator for various cardiovascular conditions. The current study aims for clarifying the link of TyG with the incidences and prognoses of cardiac arrest (CA) following acute myocardial infarction (AMI).

Methods: Our analysis is a multicenter, retrospective study utilizing data from the Medical Information Mart for Intensive Care IV and the eICU Collaborative Research Database.