TRIM47 Facilitates Osteosarcoma Progression via Destabilising FBP1 and Thus Activation of Wnt/β-Catenin Pathway.

Osteosarcoma is a malignant bone tumour with a high rate of disability and mortality in adolescents. Tripartite motif containing 47 (TRIM47) upregulation contributed greatly to carcinogenesis and progression in several tumours, while its role in osteosarcoma (OS) is still unclear and needs further investigation. In this study, we first evidenced that TRIM47 was frequently upregulated in osteosarcoma tissues and cell lines, and the higher TRIM47 expression predicted poor outcomes for osteosarcoma patients.

Learning from certain regions of interest in medical images via probabilistic positive-unlabeled networks.

The laborious annotation process and inherent image ambiguity exacerbate difficulties of data acquisition for medical image segmentation, leading to suboptimal performance in practice. This paper proposes a workaround against these challenges aiming to learn unbiased models solely from certainties. Concretely, during the labeling stage, only regions of interest confidently discerned by annotators are required to be labeled, not only increasing label quantity but also improving label quality.

Comprehensive evaluation of the PCDHB family in gastric cancer: Prognostic significance, immune infiltration, and experimental validation of PCDHB5's oncogenic role.

The protocadherin B family genes (PCDHBs) play a significant role in tumor progression, yet their specific functions in gastric cancer remain insufficiently understood. This study aims to investigate the expression patterns of PCDHBs in gastric cancer, explore their influence on immune infiltration and patient prognosis, and preliminarily validate the functional role and underlying mechanisms of PCDHB5 through in vitro experiments. Using multi-omics data from the TCGA database, we found that most PCDHBs are significantly upregulated in gastric cancer, with missense mutations being the most common mutation type.

Pan-cancer analysis of oncogenic role of MAGOH and experiment validation in hepatocellular carcinoma.

Several studies have highlighted that Mago-nashi homolog (MAGOH) contributed greatly to cell development, growth, and malignant progression in multiple cancer types. Nevertheless, the role of MAGOH in pan-cancer datasets, particularly in hepatocellular carcinoma (HCC), remains uninvestigated. In this study, we first utilized diverse databases to systematically analyze the expression and significance of MAGOH across pan-cancer.

A Bayesian deep learning model with consolidation-to-tumor ratio (CTR) prior revolutionizes the prediction of spread through air spaces (STAS) in stage IA lung adenocarcinoma: a large-scale diagnostic study.

Background: The preoperative prediction of spread through air spaces (STAS) in patients with early-stage lung adenocarcinoma (LUAD) is crucial for selecting the appropriate surgical approach and improving patient outcomes. Previous research has confirmed that there is a significant correlation between consolidation-to-tumor ratio (CTR) and STAS. This study aimed to develop a Bayesian deep learning (DL) model based on the CTR prior to predict STAS in patients with stage IA LUAD.

Yield of Advanced Neoplasia at Second Post-polypectomy Surveillance Colonoscopy: A Systematic Review and Meta-analysis.

Background & Aims: Individuals who have had polypectomy and at least one surveillance colonoscopy exam are routinely encountered, but evidence to inform risk for subsequent neoplasia and timing of surveillance is limited. Our aim was to conduct a systematic review and meta-analysis of serial surveillance yield.

Methods: We performed a systematic search of research literature databases through September 2024 and identified studies reporting neoplasia yield at baseline colonoscopy and at least 2 surveillance colonoscopies.

Identification of HCAR1 as a ferroptosis-related biomarker of gastric cancer based on a novel ferroptosis-related prognostic model and in vitro experiments.

Currently, research on ferroptosis-related prognostic models for gastric cancer is limited, whereas traditional predictive models often have a narrow perspective and low accuracy. In this study, we systematically analyzed the expression patterns of ferroptosis-related genes in patients with gastric adenocarcinoma and evaluated their prognostic value. Using data from The Cancer Genome Atlas (TCGA) and the FerrDb database, we developed a ferroptosis-related prognostic risk model based on four genes: hydroxycarboxylic acid receptor 1 (HCAR1), branched-chain amino acid transaminase 1 (BCAT1), ceruloplasmin (CP), and dickkopf-1 (DKK1).

Impact of diagnosis-intervention packet payment on the consistency of hospitalization expenses across different medical insurance schemes in China.

Background: The Diagnosis-Intervention Packet Payment (DIP) system is regarded as a localized cost-control strategy in China. It aims to improve healthcare efficiency, curb the growth of medical expenses, and optimize the allocation of medical resources among diverse groups.

Objective: This study aims to assess the impact of DIP payment reforms on differential changes in patients' hospitalization expense and to explore the degree of concentration of hospitalization expense for patients with different insurance schemes undergoing treatment for typical diseases, with a view to providing policy recommendations for improving the medical insurance system.

Engineered nanovesicles targeting SERPINE1 overcome temozolomide resistance in glioblastoma.

Glioblastoma multiforme (GBM) is a highly aggressive brain tumor with limited treatment options due to its resistance to temozolomide (TMZ). This study explores a novel therapeutic approach using engineered cell membrane nanovesicles loaded with SERPINE1 inhibitors to combat TMZ resistance. High-throughput sequencing identified pivotal genes associated with resistance, while the nanovesicles demonstrated excellent stability and the ability to cross the blood-brain barrier.

Clusterin-mediated polarization of M2 macrophages: a mechanism of temozolomide resistance in glioblastoma stem cells.

Glioblastoma remains one of the most lethal malignancies, largely due to its resistance to standard chemotherapy such as temozolomide. This study investigates a novel resistance mechanism involving glioblastoma stem cells (GSCs) and the polarization of M2-type macrophages, mediated by the extracellular vesicle (EV)-based transfer of Clusterin. Using 6-week-old male CD34 humanized huHSC-(M-NSG) mice (NM-NSG-017) and glioblastoma cell lines (T98G and U251), we demonstrated that GSC-derived EVs enriched with Clusterin induce M2 macrophage polarization, thereby enhancing temozolomide resistance in glioblastoma cells.

Natural fatal infection of Tembusu virus in bottlenose dolphins in Thailand.

Interspecies transmission of viruses poses significant risks to animal and human health. Tembusu virus (TMUV), an emerging flavivirus, is primarily associated with avian diseases. This study reports the first documented natural infection of TMUV in mammals, specifically zoo dolphins in Thailand, offering insights into its evolution, transmission dynamics, and zoonotic potential.

Task Planning of Multiple Unmanned Aerial Vehicles Based on Minimum Cost and Maximum Flow.

With the rapid development of UAV technology, UAV delivery has gained attention for its potential to reduce labor costs. However, limitations in load capacity and energy restrict UAVs' distribution capabilities. This paper proposes a cooperative delivery scheme combining traditional trucks and UAVs to extend UAV coverage and improve delivery completion rates.

SNX5 facilitates the progression of gastric cancer by increasing the membrane localization of LRP5.

Endocytosis is essential for cancer cell motility, which is predominantly mediated by the sorting nexin (SNX) family. Previous studies have demonstrated that SNX5 is elevated in several tumors, while its clinical significance and underlying mechanism in gastric cancer (GC) remain uninvestigated. In this study, we reported that SNX5 is highly expressed in GC and promotes the malignant biological behavior of GC cells.

A compendium of 8,176 bat RNA viral metagenomes reveals ecological drivers and circulation dynamics.

Bats are natural hosts for many emerging viruses for which spillover to humans is a major risk, but the diversity and ecology of bat viruses is poorly understood. Here we generated 8,176 RNA viral metagenomes by metatranscriptomic sequencing of organ and swab samples from 4,143 bats representing 40 species across 52 locations in China. The resulting database, the BtCN-Virome, expands bat RNA virus diversity by over 3.

Discovery of noncovalent diaminopyrimidine-based Inhibitors for glioblastoma via a dual FAK/DNA targeting strategy.

Temozolomide, a widely used alkylating agent for glioblastoma treatment, faces significant challenges due to the development of resistance, which severely impacts patient survival. This underscores the urgent need for novel strategies to overcome this barrier. Focal adhesion kinase (FAK), an intracellular non-receptor tyrosine kinase, is highly expressed in glioblastoma cells and has been identified as a promising therapeutic target for anti-glioblastoma drug development.

A novel fluorescent probe for rapid and selective detection of fluoride ions in living cells.

A novel fluorescent probe DTP, based on fluorine-silicon complexation, extends emission to 590 nm and achieves a 5 minutes response time. It shows high selectivity and a 0.98 μM detection limit for fluoride ions, with successful bioimaging application in living cells.

Development of a novel fluorescent probe for the Selective and sequential imaging of fluoride and hypochlorite.

Fluoride (F) are common in the environment and are beneficial in moderate amounts but can be harmful at high concentrations, leading to conditions such as fluorosis influenced by reactive oxygen species (ROS), including hypochlorite (ClO). This study introduces PZ-DT, a novel fluorescent probe with a phenothiazine core, designed for the sequential detection of F and ClO. PZ-DT emits orange-red fluorescence with a Stokes shift greater than 200 nm upon F detection, demonstrating excellent selectivity and sensitivity, boasting a quantum yield of 19.

Design, synthesis and antitumor activity of 4-indazolylpyrimidine derivatives as EGFR inhibitors.

To overcome T790M mutation, a novel series of 4-indazolypyrimidine derivatives were developed as novel EGFR inhibitors employing a scaffold hopping drug design strategy. The biological activities of the target compounds were evaluated against two tumor cell lines (A431 and NCI-H1975), normal cell 2BS and EGFR kinase. The results indicated that the majority of the compounds exhibited promising antitumor activity and low toxicity.

Viromics-based precision diagnosis of reproductive abnormalities in cows reveals a reassortant Akabane disease virus.

Background: At the end of 2021, an epidemic of reproductive abnormalities in cows occurred in Jilin Province, China, posing an urgent need for a rapid diagnosis.

Results: To identify the cause of the disease, a total of 172 samples were collected from 21 dead calves and 45 aborting or pregnant cows in 10 farms across the province. Routine PCR or RT-PCR detection did not find any common abortion-related agents.

The E3 ubiquitin ligase RNF6 facilitates the progression of cervical cancer by inhibiting the Hippo/Yap pathway.

Purpose: Cervical cancer (CC), a significant global health threat, necessitates comprehensive understanding for improved therapeutic interventions. Many research indicates that dysregulation of the Hippo-YAP1 pathway leads to uncontrolled proliferation and invasion of tumor cells, promoting the progression of various cancers. This article aims to elucidate the role of RNF6 in CC and its regulation of the Hippo-YAP1 signaling pathway.

SPIN1 accelerates tumorigenesis and confers radioresistance in non-small cell lung cancer by orchestrating the FOXO3a/FOXM1 axis.

Despite the importance of radiation therapy as a nonsurgical treatment for non-small cell lung cancer (NSCLC), radiation resistance has always been a concern because of poor patient response and outcomes. Therefore, it is crucial to identify novel targets to increase the effectiveness of radiotherapy and investigate the mechanisms underlying radioresistance. Previously, we demonstrated that Spindlin 1 (SPIN1) was related to tumour initiation and progression.

Insights into the unique roles of extracellular vesicles for gut health modulation: Mechanisms, challenges, and perspectives.

Extracellular vesicles (EVs), which play significant regulatory roles in maintaining homeostasis and influencing immune responses, significantly impact gut microbiota composition and function, affecting overall gut health. Despite considerable progress, there are still knowledge gaps regarding the mechanisms by which EVs, including plant-derived EVs (PDEVs), animal-derived EVs (ADEVs), and microbiota-derived EVs (MDEVs), modulate gut health. This review delves into the roles and mechanisms of EVs from diverse sources in regulating gut health, focusing on their contributions to maintaining epithelial barrier integrity, facilitating tissue healing, eliciting immune responses, controlling pathogens, and shaping microbiota.

A Lipase Gene of : Sequence Analysis and High-Efficiency Expression in .

Lipase, a type of enzyme that decomposes and synthesizes triglycerides, plays an important role in lipid processing. In this study, a heat-resisting lipase gene (4) from was subcloned into the pPICZαA vector and then transformed into X33. The recombinant yeast cell concentration reached the maximum (119.

TBK1 is involved in M-CSF-induced macrophage polarization through mediating the IRF5/IRF4 axis.

TANK binding kinase 1 (TBK1) is an important kinase that is involved in innate immunity and tumor development. Macrophage colony-stimulating factor (M-CSF) regulates the differentiation and function of macrophages towards the immunosuppressive M2 phenotype in the glioblastoma multiforme microenvironment. The role of TBK1 in macrophages, especially in regulating macrophage polarization in response to M-CSF stimulation, remains unclear.