The -124C>T Mutation of the Promoter Indicates Favorable Prognosis in Ovarian Clear Cell Carcinoma: A Single Institutional Study in China.

Ovarian clear cell carcinoma (OCCC) is characterized by chemoresistance and poor prognosis in advanced or recurrent cases. This study aimed to find specific prognostic markers for OCCC. We analyzed 169 OCCC patients for clinicopathological features.

A new digital biomarker of blepharitis: energy curve of the meibomian edge.

Purpose: To develop and validate a novel digital biomarker, the energy curve of the meibomian gland (MG) edge, to assess MG uneven atrophy and aid in diagnosing blepharitis.

Methods: A retrospective study enrolled 76 dry eye patients (42 with blepharitis, 34 controls). Segmentation of upper eyelid meibography images was accomplished via a convolutional neural network (CNN)-based artificial intelligence (AI) model.

NHC-Catalyzed Enantioselective Access to Pyrazoles with a Nitrile-Containing All-Carbon Quaternary Center.

Chiral nitriles are valuable structural motifs found in numerous pharmaceuticals and natural products. This study presents an efficient method for synthesizing chiral nitriles via a cycloaddition reaction between -cyano-substituted ,-unsaturated aldehydes and 3-methyl-1-phenyl-1-pyrazole-5-amine. The transformation is catalyzed by -heterocyclic carbene (NHC) at low loadings, affording the corresponding chiral nitriles in good to excellent yields with excellent enantioselectivities.

Prevalence and risk factors of compassion fatigue in gynecological and obstetric nurses: a systematic review and meta-analysis.

Objective: This study systematically evaluated the incidence and influencing factors of compassion fatigue (CF) among obstetric and gynecological nurses.

Methods: Retrieve the cross-sectional study on the current occurrence status of CF among obstetric and gynecological nurses in the Web of Science, PubMed, Embase, Cochrane Library, CINAHL, CBM, CNKI, VIP and WANFANG database. Database were searched from the database establishment to September 8, 2024.

BLIMP1 negatively regulates IL-2 signaling in T cells.

Interleukin-2 (IL-2) regulates immune homeostasis by fine-tuning the balance between effector and regulatory T (T) cells. To identify regulators of IL-2 signaling, we performed genome-wide CRISPR-knockout screening in IL-2-dependent cells derived from a patient with adult T cell leukemia (ATL) and found enrichment of single guide RNAs targeting , which encodes B lymphocyte-induced maturation protein 1 (BLIMP1). BLIMP1 inhibits IL-2 production by T cells; however, its role in IL-2 signaling remains unknown.

Spatio-temporal Multi-granularity for Skeleton-based Depression Risk Recognition.

As the prevalence of depression continues to rise, the timely and accurate recognition of its early signs is crucial for effective prevention and intervention. However, current clinical diagnostic methods are limited by the absence of objective biomarkers and inefficiencies in early recognition. Recent research has revealed a significant correlation between gait patterns and depression risk, suggesting that gait analysis could serve as a promising tool for early diagnosis.

The broad-spectrum antimicrobial peptide BMAP-27B potentiates carbapenems against NDM-producing pathogens in food animals.

The emergence and spread of antibiotic-resistant pathogens in food animals pose a major threat to global public health. Carbapenem-resistant (CRE), particularly those producing New Delhi Metallo-β-lactamase (NDM-CRE), are prevalent in livestock and have acquired resistance to nearly all commonly used β-lactam antibiotics. This study evaluated the efficacy of the antimicrobial peptide BMAP-27B, a derivative of the cathelicidin family, against NDM-CRE strains in food animals.

Ultrasound-based machine learning model to predict the risk of endometrial cancer among postmenopausal women.

Background: Current ultrasound-based screening for endometrial cancer (EC) primarily relies on endometrial thickness (ET) and morphological evaluation, which suffer from low specificity and high interobserver variability. This study aimed to develop and validate an artificial intelligence (AI)-driven diagnostic model to improve diagnostic accuracy and reduce variability.

Methods: A total of 1,861 consecutive postmenopausal women were enrolled from two centers between April 2021 and April 2024.

Isolation and Identification of YJ-2 from the Rhizosphere Soil of to Explore Its Potential as a Biocontrol Agent Against Plant Fungal Diseases.

Plant fungal diseases remain a major threat to global agricultural production, necessitating eco-friendly and sustainable strategies. Conventional chemical fungicides often lead to the development of resistant pathogen strains and cause environmental contamination. Therefore, the development of biocontrol agents is particularly important.

Mutational landscape and DNA methylation-based classification of squamous cell carcinoma and urothelial carcinoma.

Background: Identification of the tissue of origin is fundamental for cancer treatment. However, squamous cell carcinomas from different sites lack representative histological and immunohistochemical features. This study aimed to identify mutational profiles and further establish a DNA methylation-based classification for squamous cell carcinoma and urothelial carcinoma.

Karyotyping with amniotic fluid in 6,572 pregnant women and pregnancy outcomes--A single-center retrospective study.

Aims: To detect abnormal chromosome karyotypes in amniotic fluid cells and to explore the relationship among various prenatal diagnostic indications, karyotypes, and pregnancy outcomes.

Methods: The data used in this study were obtained from 6,572 pregnant women at high risk for fetal chromosomal abnormalities who visited the Luzhou Prenatal Diagnostic Center for amniocentesis from January 2017 to February 2023. The data were accessed from May to October 2023.

Trace detection of natural gas leakage using novel hollow-electrode radiofrequency glow discharge ion-source mass spectrometry.

Natural gas has become an indispensable energy source for people's lives, but natural gas leakage brings great harm to people's health and property as well as the atmospheric environment. The current ion source cannot meet the requirements of trace natural gas leakage detection due to the interference of air when the sample is injected at atmospheric pressure, and the whole detection process is time-consuming, so rapid on-line detection is not possible. In this paper, a novel hollow electrode radio frequency glow discharge ion source is designed for direct injection of samples at atmospheric pressure.

A Bayesian network meta-analysis of EGFR-tyrosine kinase inhibitor treatments in patients with mutation-positive non-small cell lung cancer.

Background: To date, no direct comparisons have been performed to compare the effectiveness of all epidermal growth factor receptor-tyrosine kinase inhibitors (EGFR-TKIs) against mutation-positive non-small cell lung cancer (NSCLC). This study aimed to investigate the efficacy and safety of EGFR-TKIs in patients with mutation-positive NSCLC.

Methods: We conducted a network meta-analysis of randomized controlled trials comparing osimertinib, lazertinib, aumolertinib, befotertinib, furmonertinib, dacomitinib, afatinib, erlotinib, gefitinib, icotinib, and chemotherapy.

Noninvasive Detection of Macrophages in Atherosclerotic Lesions Using a Visipaque-Based Nanoparticle Contrast Agent for Computed Tomography.

This study aimed to evaluate iodinated nanoparticles based on Visipaque for the detection of macrophages in atherosclerotic plaques using computed tomography (CT). The nanoparticles were developed using Visipaque and hydrophobic groups to enhance the macrophages in atherosclerotic lesions. The nanoparticles were measured using NanoSight, and their cellular toxicity was evaluated using the cell counting kit-8 assay.

The genome and GeneBank genomics of allotetraploid Nicotiana tabacum provide insights into genome evolution and complex trait regulation.

Nicotiana tabacum is an allotetraploid hybrid of Nicotiana sylvestris and Nicotiana tomentosiformis and a model organism in genetics. However, features of subgenome evolution, expression coordination, genetic diversity and complex traits regulation of N. tabacum remain unresolved.

Care guided by tissue oxygenation and haemodynamic monitoring in off-pump coronary artery bypass grafting (Bottomline-CS): assessor blind, single centre, randomised controlled trial.

Objective: To assess whether perioperative management guided by near-infrared spectroscopy to determine tissue oxygen saturation and haemodynamic monitoring reduces postoperative complications after off-pump coronary artery bypass grafting.

Design: Assessor blinded, single centre, randomised controlled trial (Bottomline-CS trial).

Setting: A tertiary teaching hospital in China.

Cationic starch styrene acrylic antibacterial emulsion based on subject object recognition of β-cyclodextrin for paper surface modification.

There are some drawbacks such as the propagation and spread of bacteria and viruses during the use of normal paper. Therefore, this work designed a starch styrene-acrylic antibacterial emulsion for improving the paper properties. The modified antimicrobial monomer was prepared by the subject-object recognition of β-cyclodextrin with hydrophobic modified titanium dioxide.

Identification and functional validation of ACSL1 as a biomarker regulating ferroptosis in nucleus pulposus cell.

Intervertebral disc degeneration (IVDD) is a prevalent musculoskeletal disorder characterized by the deterioration of nucleus pulposus (NP) cells, leading to significant impairments in patients' quality of life. Elucidating the molecular mechanisms underlying IVDD is essential for developing effective therapeutic strategies. In this study, we utilized weighted gene co-expression network analysis to identify key module eigengenes (MEs) from the GSE124272 dataset, combined with differential gene expression analysis to pinpoint differentially expressed genes (DEGs).

Coronavirus Disease 2019 Case Series in China: Sequelae and Effectiveness of Vaccination and Antiviral Drugs.

Purpose Of The Study: This study investigated post-coronavirus disease 2019 (COVID-19) sequelae among a sample of the Chinese population, and determined the statistical significance of correlations between age, sex, number of COVID-19 vaccinations, number of SARS-CoV-2 infections, development of pneumonia, use of specific drugs (namatevir/ritonavir, azvudine, molnupiravir), chronic underlying diseases, and post-COVID-19 sequelae.

Methods: Data from 869 patients, who visited the Emergency Department of Beijing Shijitan Hospital (Beijing, China) between December 7, 2022 to January 31, 2024, were collected. The criteria for admission: Age ≥ 14 years old, and diagnosed with ≥ 1 positive result on nucleic acid or antigen tests.

An accurate DNA and RNA based targeted sequencing assay for clinical detection of gene fusions in solid tumors.

Gene fusions are one of the most important molecular biomarkers for tumor diagnosis, classification and targeted therapy. How to accurately detect them is a key issue in clinical work. In this study, a custom-designed integration of DNA and RNA-based next generation sequencing (NGS) assay including 16 targeted therapy related genes was developed and validated to identify gene fusions in solid tumors.

Exploring the Roles of the () Gene Family in Tomato ( L.) Under Abiotic Stresses.

PLATZ transcription factors represent a novel class of zinc finger proteins unique to plants and play critical roles in plant growth and stress responses. This study performs a bioinformatic analysis on the PLATZ transcription factor family in tomato. In the tomato genome, 20 PLATZ transcription factors were identified, distributed across nine chromosomes, including two tandem duplication clusters and two segmental duplication events.

Whole-Genome Sequencing and Fine Map Analysis of .

(T. Ito) S. Ito and S.

DNA methylation confers epigenetic changes in cold-adapted microorganisms in response to cold stress.

DNA methylation modification regulates gene expression during temperature stress. The adaptation mechanisms of cold-adapted microorganisms to low temperatures have been explained at the gene and metabolic levels. However, considering the important epigenetic modification in cells, the role of genomic modification in cold-adapted microorganisms remains underexplored.

Ultrasound Evaluation of the Changes of Ophthalmic Artery Doppler and Optic Nerve Sheath in Pregnant Women With FGR.

Objectives: This study aimed to compare changes in ultrasonic Doppler parameters, particularly the peak ratio (PR) of the ophthalmic artery (OA) and optic nerve sheath diameter (ONSD), in pregnancies complicated by fetal growth restriction (FGR). Furthermore, it sought to evaluate differences in these parameters across various subgroups and analyze the cut-off value of PR for predicting delivery within 1 week in pregnancies complicated by FGR.

Methods: A total of 62 pregnant women in the mid-to-late stages of gestation were enrolled, comprising 31 participants in the FGR group and 31 in the control group.

Neoadjuvant immunotherapy for DNA mismatch repair proficient/microsatellite stable non-metastatic rectal cancer: a systematic review and meta-analysis.

Background: Neoadjuvant immunotherapy (NIT) has been endorsed by clinical guidelines for the management of DNA mismatch repair deficiency/microsatellite instability-high (dMMR/MSI-H) locally advanced rectal cancer (LARC). Nonetheless, the therapeutic efficacy of NIT in mismatch repair-proficient/microsatellite stable (pMMR/MSS) non-metastatic rectal cancer (RC) remain pending matters. Therefore, a meta-analysis was carried out to assess the efficacy and safety of NIT in patients with non-metastatic pMMR/MSS RC.