Genome-wide association study and fine-mapping using imputed sequences to prioritize candidate genes for 30 complex traits in 50,309 Holstein bulls.

Identifying causal genetic variants underlying economically important traits in dairy cattle is essential for understanding their genetic basis and optimizing breeding programs. The growing availability of sequenced reference genomes and individuals with both phenotypic and genotypic data notably enhances our ability to detect genetic associations and further pinpoint causal effects. This comprehensive GWAS of dairy cattle used deregressed breeding values as phenotypes and analyzed 11,292,243 quality-controlled, imputed sequence variants from 50,309 Holstein bulls.

Arbuscular mycorrhizal association regulates global root-seed coordination.

Terrestrial plants exhibit immense variation in their form and function among species. Coordination between resource acquisition by roots and reproduction through seeds could promote the fitness of plant populations. How root and seed traits covary has remained unclear until our analysis of the largest-ever compiled joint global dataset of root traits and seed mass.

Ligand supplementation restores the cancer therapy efficacy of the antirheumatic drug auranofin from serum inactivation.

Auranofin, an FDA-approved antirheumatic gold drug, has gained ongoing interest in clinical studies for treating advanced or recurrent tumors. However, gold ion's dynamic thiol exchange nature strongly attenuates its bioactivity due to the fast formation of covalent albumin-gold adducts. Here we report that newly-added thiols can modulate the dynamic albumin-gold binding and recover the therapeutic efficacy.

Benchmarking of feed-forward neural network models for genomic prediction of quantitative traits in pigs.

Artificial neural networks are machine learning models that have been applied to various genomic problems, with the ability to learn non-linear relationships and model high-dimensional data. These advanced modeling capabilities make them promising candidates for genomic prediction by potentially capturing the intricate relationships between genetic variants and phenotypes. Despite these theoretical advantages, neural networks have shown inconsistent performance across previous genomic prediction research, and limited studies have evaluated their performance and feasibility specifically for pig genomic predictions using large-scale data.

Heterologous Expression and Antimicrobial Mechanism of a Cysteine-Rich Peptide from Barnacle .

The escalating crisis of antimicrobial resistance in aquaculture, driven by the indiscriminate use of antibiotics, underscores the urgent need to develop novel anti-infective agents. This study addresses this requirement by investigating cysteine-rich antimicrobial peptides (AMPs) in understudied crustacean species. A cysteine-rich AMP, designated Rcys1, was identified and characterized from the genome of .

Increased dependence on mycorrhizal fungi for nutrient acquisition under carbon limitation by tree girdling.

Nutrient acquisition through symbiotic ectomycorrhizal fungi is carbon (C) costly but fundamental for plant growth, community, and ecosystem functioning. Here, we examined the functions of roots and mycorrhiza with respect to nutrient uptake after artificially inducing C limitation-seven months after girdling of an ectomycorrhizal tree, . Root physiological activity (measured as root nitrogen content and root exudation) declined after girdling and was accompanied with 110% and 340% increases in mycorrhizal colonization and extramatrical hyphal length, respectively.

Phase separation of RXRγ drives tumor chemoresistance and represents a therapeutic target for small-cell lung cancer.

Small-cell lung cancer (SCLC) is the most lethal type of lung cancer, characterized by rapid evolution from chemosensitivity to chemoresistance and limited treatment options. However, the mechanisms underlying this evolution remain poorly understood. Here, we show that Retinoid X receptor γ (RXRγ) is uniquely overexpressed in chemo-resistant SCLC tumors, and that RXRγ serves as an essential factor driving chemoresistance in SCLC.

Effects of color temperature of visible light camera on vegetation index calculation in unmanned aerial vehicle vegetation remote sensing.

Vegetation indices based on the spectral information of the RGB three-bands have been widely used in the field of unmanned aerial vehicles (UAVs) vegetation remote sensing. Affected by the automatic color temperature adjustment of cameras, the phenomena of color cast often occur when UAVs acquire RGB images. However, its impacts on the calculations of vegetation indices remain largely unknown.

Characteristics and potential diagnostic value of gut microbiota in ovarian tumor patients.

The gut microbiota is closely related to the occurrence and development of cancer. However, the characteristics of gut microbiota associated with ovarian tumors remain elusive. In this study, fecal samples were collected from healthy control (HC) group and patients with ovarian tumor (OT) or with other benign tumor (OBT) for 16s rRNA sequencing to determine differential flora in gut microbiota.

Size-dependent internalization of polystyrene microplastics as a key factor in macrophages and systemic toxicity.

Microplastics are emerging pollutants with a wide range of ecological and biological effects, including the ability to accumulate in organisms and induce toxicity. Although numerous studies have investigated the distribution and toxicity of microplastics in murine models and cell lines, the conclusions are inconsistent owing to variations in experimental designs, particle sizes, exposure methods, and dose quantifications. To address these gaps, we systematically evaluated the size-dependent internalization and toxicity of polystyrene microplastics (PS-MPs) using in vitro and in vivo models.

From Quencher to Promoter: Revisiting the Role of 2,4,6-Trimethylphenol (TMP) in Triplet-State Photochemistry of Dissolved Organic Matter.

Triplet-state dissolved organic matter (DOM*) plays a crucial role in environmental aquatic photochemistry, with 2,4,6-trimethylphenol (TMP) frequently used as a chemical probe or quencher due to its high reactivity with DOM*. However, the influence of TMP-derived oxidation intermediates on the target photochemical reactions has not been comprehensively examined. This study investigated TMP's effect on the photolysis of sulfamethoxazole (SMX), a common antibiotic found in natural waters, in the presence of different DOM sources or model photosensitizer.

Heating-Induced Changes in Content and Molecular Characteristics of Pyrogenic Dissolved Organic Matter across Soil Types.

Wildfires remarkably alter the quantity and quality of dissolved organic matter (DOM) that regulates postfire biogeochemical processes and environmental quality. However, it remains unclear how the heating-induced percent changes (%HIC) in DOM quantity and quality differ among soil types on a wide geographic scale. Here, we used dissolved organic carbon (DOC) quantification, absorption, and fluorescence spectroscopies, and Fourier transform ion cyclotron resonance mass spectrometry to investigate the variations in %HIC in DOM quantity and quality of Chinese soil reference materials after heating at 250 and 400 °C.

Marine Recalcitrant Dissolved Organic Matter Gained by Processing at Sandy Subterranean Estuaries.

The sandy subterranean estuary (STE) connecting fresh groundwater to saline sea water is characterized by strong geochemical (salinity, redox, and pH) gradients, with evidence emerging for its role as a hot spot for consumption of labile substrates. This inspired us to conduct a study to evaluate whether this holds true for dissolved organic matter (DOM), especially given the still mysterious origin of marine recalcitrant DOM. Here, characterization of DOM of 21 groundwater samples (depth 1-13 m, salinity 3.

Forest Soil pH and Dissolved Organic Matter Aromaticity Are Distinct Drivers for Soil Microbial Community and Carbon Metabolism Potential.

The ecological niche separation of microbial interactions in forest ecosystems is critical to maintaining ecological balance and biodiversity and has yet to be comprehensively explored in microbial ecology. This study investigated the impacts of soil properties on microbial interactions and carbon metabolism potential in forest soils across 67 sites in China. Using redundancy analysis and random forest models, we identified soil pH and dissolved organic matter (DOM) aromaticity as the primary drivers of microbial interactions, representing abiotic conditions and resource niches, respectively.

Small protein ERSP encoded by LINC02870 promotes triple negative breast cancer progression via IRE1α/XBP1s activation.

Clinical treatment options for triple-negative breast cancer (TNBC) are currently limited to chemotherapy because of a lack of effective therapeutic targets. Recent evidence suggests that long noncoding RNAs (lncRNAs) encode bioactive peptides or proteins, thereby playing noncanonical yet significant roles in regulating cellular processes. However, the potential of lncRNA-translated products in cancer progression remains largely unknown.

Multicenter study of ovarian cancer score for diagnosing ovarian cancer.

Background: Early detection is crucial for improving survival of patients with ovarian cancer (OC), yet current diagnostic tools lack adequate sensitivity and specificity, especially for early stage disease. The study aimed to validate the serum small extracellular vesicles (sEV) protein based Ovarian Cancer Score (OCS) in detecting OC.

Methods: This multicenter study included 1183 adult females with adnexal masses from four hospitals in China (October 2019-April 2023).

Discovery and Characterization of an Atypical Crustin Antimicrobial Peptide from .

Crustins are a family of antimicrobial peptides (AMPs) that play a pivotal role in the innate immune system of crustaceans. The discovery of novel AMPs from natural sources is crucial for expanding our current database of these peptides. Here, we identified and characterized a novel member of the crustin family, named Crus-SWD1, derived from .

Molecular-level insight into the role of soil-derived dissolved organic matter composition in regulating photochemical reactivity.

Soil-derived dissolved organic matter (DOM) links soil and water carbon pools and is an important source of photochemically produced reactive intermediates (PPRIs) in aquatic environments. Despite its importance, the variations in photochemical reactivity of soil-derived DOM molecules in producing PPRIs across broad geographical regions, and the factors driving these variations, remain unclear. Herein, we resolved the apparent quantum yields (Φ(PPRIs)) of hydroxyl radicals (•OH), singlet oxygen (O), and excited triplet-state DOM (DOM*) for irradiated DOM from 22 representative soil reference materials in China, and linked them to soil pH, mineral weathering degree, and DOM characteristics.

Design, Synthesis, and Biological Evaluation of Novel Diaminopyrimidine Macrocycles as Fourth Generation Reversible EGFR Inhibitors That Overcome Clinical Resistance to Osimertinib Mediated by C797S Mutation.

Overcoming clinical resistance to osimertinib mediated by the tertiary C797S mutation remains an unmet medical need. To date, there are no effective drugs that have been approved for patients who harbor EGFR mutations. Herein, we applied a structure-based drug design strategy to discover a series of potent and selective diaminopyrimidine macrocycles as novel EGFR inhibitors.

Effect of moisture on mechanical and physical properties of coals: a uniaxial compression study.

The estimation of mechanical and physical properties of coal reservoirs is important for the successful exploration and development of coalbed methane (CBM). Unlike conventional sandstone reservoirs, coal reservoirs exhibit greater sensitivity to stress, resulting in distinct mechanical and physical behaviors. In this study, uniaxial compression tests were performed on both low-rank and high-rank coal samples under different moisture conditions to reveal the mechanical and physical property changes with stress.

Accelerometer-Derived Physical Activity, Sedentary Behavior, and the Risk of Depression and Anxiety in Middle-aged and Older Adults: A Prospective Cohort Study of 71,556 UK Biobank Participants.

Objectives: To investigate the associations between accelerometer-measured physical activity and sedentary behavior with depression and anxiety.

Methods: We used accelerometer data from the UK biobank. Time spent in moderate-to-vigorous physical activity (MVPA) was classified into four categories: very-low (0-74.

Therapeutic targeting ERRγ suppresses metastasis via extracellular matrix remodeling in small cell lung cancer.

Small-cell lung cancer (SCLC) is the most aggressive and lethal type of lung cancer, characterized by limited treatment options, early and frequent metastasis. However, the determinants of metastasis in SCLC are poorly defined. Here, we show that estrogen-related receptor gamma (ERRγ) is overexpressed in metastatic SCLC tumors, and is positively associated with SCLC progression.