Single-incision laparoscopic cholecystectomy with inflexible laparoscopic instruments and laparoscopy: a single-center experience of 533 cases.

Background: Laparoscopic cholecystectomy (LC) has been widely performed as the gold standard for BGDs. Single-incision laparoscopic cholecystectomy (SILC) was considered as an option for minimizing surgical injuries and improving outcomes. However, the benefit of this novel technique, especially with conventional and inflexible instruments and laparoscopy, is still controversial.

High-Nuclearity Copper Molecular Catalysts for Electrocatalytic CO-to-Acetate Conversion.

Ligand-modified metal nanoclusters (NCs) have emerged as candidate materials for catalysis owing to their well-defined yet tunable structure and their metal centers' high nuclearity. We posited that NC-based catalytic behavior will depend on ligand properties, the accessibility of active sites, and their atomic configuration. We synthesized a series of Cu NC-based catalysts, tuned local hydrophobicity through ligand adjustment, balanced the ligand coverage and active site exposure, and found that we were, in this way, able to engender efficient electrosynthesis of acetate via CO electroreduction.

Fermented purple potato dough improves glycolipid metabolism by modulating gut microbiota and fecal metabolites in high-fat diet-induced obese mice.

Obesity is one of the major global public health concerns. Purple potatoes exhibit anti-obesity properties attributable to their content of abundant polyphenols and amylose. However, the anti-obesity effects and underlying mechanisms of purple potato-based staple foods remain to be fully elucidated.

Mechanisms of Senegenin in Regulating Oxidative Stress and Mitochondria Damage for Neuroprotection in Insomnia: Evidence from In Vivo and In Vitro Models.

Insomnia is a common sleep disorder worldwide, and oxidative stress and mitochondrial damage are closely related to insomnia. This study aimed to investigate the mechanism by which senegenin exerts neuroprotective effects in regulating oxidative stress and mitochondrial damage in insomnia. In vivo, EEG/EMG analysis confirmed the successful establishment of insomnia rat models; Nissl and HE staining and electron microscopy were used to evaluate the pathological changes of neurons and mitochondria in rat brain tissue.

Targeting Catenibacterium mitsuokai with icariin modulates gut microbiota and improves hepatic lipid metabolism in intrauterine growth restriction.

Male offspring with intrauterine growth restriction (IUGR) exhibit more pronounced hepatic lipid metabolism abnormalities than females, necessitating earlier intervention. Icariin (ICA) has been shown to effectively modulate hepatic lipid metabolism in male piglets with IUGR. However, the role of gut microbiota in this process remains to be elucidated.

Reference equations for pulmonary function testing in healthy Chinese children aged 4-18 years.

Background: Accurate spirometry reference equations are essential for diagnosing and managing respiratory conditions in children. Although the GLI Global Equations have been proposed for general use, there were few Asian populations available for inclusion. This study aims to develop and validate spirometric reference equations for healthy Chinese children.

Investigating the properties of potato protein fibers and their application in oleogel -structured high internal phase emulsions.

Recently, the fiberization of protein has attracted interest as the it has the ability to improve properties of proteins. This work aimed to investigate the characteristics and mechanism of self-assembly of potato protein (PP) into protein fibers (PNFs) under certain conditions. The properties of potato protein fibers and gums (PP-PNFs/GA) were investigated.

Comprehensive Review on Oleogels Structured by Lipid-Based Compounds: From Structure Mechanisms to Nutritional Functionalities and Applications in Food Industry.

Nowadays, oleogels have gained attentions as promising fat substitutes. Lipid-based compounds, mono- and diacylglycerols, fatty acids (FAs), fatty alcohols, waxes, sterols, ceramides, and phospholipids exhibit excellent oil-gelling potential due to their structural similarity to triacylglycerols. This review addresses how oleogels composition affects their nutritional functionality and further applications in food industry.

Enhanced Stress Tolerance in Rice Through Overexpression of a Chimeric Glycerol-3-Phosphate Dehydrogenase (OEGD).

Crop productivity is severely constrained by abiotic and biotic stresses, necessitating innovative strategies to enhance stress resilience. Glycerol-3-phosphate (G3P) is a central metabolite in carbohydrate and lipid metabolism, playing crucial roles in stress responses. In this study, we engineered a novel () gene, designated , by fusing the N-terminal NAD-binding domain of rice with the feedback-resistant C-terminal catalytic domain of .

Scaled CO Electroreduction to Alcohols.

Electrocatalysis offers a promising route to convert CO into alcohols, which is most efficient in a two-step cascade reaction with CO-to-CO followed by CO-to-alcohol. However, current alcohol-producing CO/CO electrolyzers suffer from low selectivity or alcohol crossover, resulting in alcohol concentrations of less than 1%, which are further diluted in downstream cold-traps. As a result, electrocatalytic alcohol production has yet to be scaled beyond the lab (1-10 cm).

Preparation of Calcium-Chelating Peptides from Squid Skin and Evaluation of Calcium Absorption Capacity in Caco-2 Cell Monolayer Model.

To develop a highly bioavailable calcium supplement, this study utilized Peruvian squid () skin as a raw material. Through alkaline protease hydrolysis and enzymatic membrane reactor separation, three molecular weight fractions of squid skin peptides were obtained, followed by calcium ion chelation to synthesize calcium-chelating peptides (CCPs-SS). Systematic characterization revealed that the less than 1 kDa fraction of CCPs-SS exhibited superior antioxidant capacity (82.

Effect of Low-Temperature Plasma Sterilization on the Quality of Pre-Prepared Tomato-Stewed Beef Brisket During Storage: Microorganism, Freshness, Protein Oxidation and Flavor Characteristics.

Traditional tomato-braised beef brisket with potatoes is celebrated for its rich, complex flavors and culinary appeal but requires lengthy preparation. Pre-packaged versions of the dish rely on thermal sterilization for safety; however, high-temperature processing accelerates protein and lipid oxidation, thereby compromising its sensory quality. As the demand for ready-to-eat meals grows, the food industry faces the challenge of ensuring microbial safety while preserving flavor integrity.

Engineering Patatin for enhanced lipase activity and long-chain fatty acid specificity via rational design.

Patatin, a multifunctional glycoprotein from potatoes, is a promising lipase for industrial applications due to its emulsifying, antioxidant, and lipid-modifying properties. However, its low expression efficiency and preference for short-chain substrates limit practical utility. Here, we addressed these challenges by heterologously expressing patatin in Pichia pastoris X-33, achieving a yield of 121 mg/L through optimized fermentation.

Elucidation of formation and development of warmed-over flavor in precooked surimi products as affected by reheating methods.

This study investigated reheating methods (water bath, WB; steam, SM; microwave, MW) on formation and development of undesired warmed-over flavor (WOF) in precooked surimi products. MW resulted in shrinkage of surimi gel, while SM caused large pores on disrupted structure. Unsaturated fatty acids, mainly oleic (308.

The transcription factor TCF4 regulates the miR-494-3p/THBS1 axis in the fibrosis of pathologic scars.

Background: The fibrosis of pathologic scar (PS) is formed by the excessive deposition of extracellular matrix, resulting in an abnormal scar. Recent clinical tests have indicated that the regulation of PS fibroblast cells (PSF cells) proliferation can serve as an intervention measure for PS. Our work aimed to elucidate the specific mechanism of action of TCF4 on the progression of PS fibrosis.

The application of omalizumab in children with atopic diseases complicated by nephrotic syndrome: a case report.

Nephrotic syndrome (NS) and atopic diseases are linked through shared immunological pathways, with allergic triggers often contributing to NS relapses, particularly in immunoglobulin E (IgE)-mediated pathways. Omalizumab, a humanized monoclonal antibody targeting free and cell-bound IgE, is commonly used in treating atopic diseases. We presented a pediatric case with a history of eczema, asthma, and recurrent atopic rhinitis, who first developed NS at age three, responding well to steroid therapy.

Development of 3D Printable Silver Carp () Surimi Gel with Dynamic High-Pressure Microfluidization-Modified Pea Protein Isolate and Microcrystalline Cellulose.

Sliver carp is a nutritious and abundant species in China, but its low market value stems from its thin meat, small bones and strong odor. Processing it into surimi enhances its economic value, though surimi typically has low gel strength and is prone to deterioration. Recently, three-dimensional (3D) printing has gained attention as an innovative additive manufacturing technique for personalization and process simplification requiring high-performance materials.

Multi-scale structure and digestible process of wheat starch as affected by distribution behavior of rice glutelin amyloid fibril aggregates during gelatinization and digestion.

The effect of distribution behaviors of rice glutelin amyloid fibril aggregates (RAFA) on the structures and digestible process of wheat starch was investigated, and the interaction was revealed by molecular dynamics simulations. Rice glutelin (RG)/RAFA enhanced the long-range ordered structure of starch, and the relative crystallinity of gelatinized RAFA-wheat starch reached 14.35 %.

Effect and mechanism of allergen-specific immunotherapy on small airway dysfunction in children with asthma.

Background: The purpose of this study was to investigate the effectiveness of allergen-specific immunotherapy (AIT) on small airway dysfunction (SAD) and the underlying mechanism with a special focus on basophils.

Methods: Sixty-five children with mild to moderate asthma who were under regular inhaled corticosteroid (ICS) treatment for more than 1 year but whose FEF remained below 65% of the predicted value and had positive results for serum Der p or Der f were enrolled. Children with asthma underwent house dust mite (HDM) subcutaneous immunotherapy (SCIT) treatment for 1 year.

Senegenin regulates the mechanism of insomnia through the Keap1/Nrf2/PINK1/Parkin pathway mediated by GAD67.

GAD67 impacts insomnia as a key enzyme catalysing the conversion of glutamate (Glu) to gamma-aminobutyric acid (GABA). Senegenin enhances neuroprotection and is used widely to treat insomnia and other neurological diseases. This study aimed to investigate how senegenin regulates insomnia through a GAD67-mediated signalling pathway.

Formulation of novel low-sodium salts using potassium salts and dietary polysaccharide.

Potassium citrate (KC) and potassium lactate (KL) are considered as salt replacers due to their saltiness, processing advantages, and health benefits. However, the obvious bitter taste associated with these compounds has limited their use in salt substitutes. Despite this challenge, little attention has been paid to improving their sensory properties.

Exploring the prevalence and chest CT predictors of Long COVID in children: a comprehensive study from Shanghai and Linyi.

Introduction: COVID-19 constitutes a pandemic of significant detriment to human health. This study aimed to investigate the prevalence of Long COVID following SARS-CoV-2 infection, analyze the potential predictors of chest CT for the development of Long COVID in children.

Methods: A cohort of children who visited the respiratory outpatient clinics at Shanghai Children's Medical Center or Linyi Maternal and Child Health Care Hospital from December 2022 to February 2023 and underwent chest CT scans within 1 week was followed up.

Association of obesity with osteoporotic fracture risk in individuals with bone metabolism-related conditions: a cross sectional analysis.

Introduction: This study aimed to investigate the individual and composite associations of different indices of obesity on osteoporotic fractures at three different sites among individuals affected by conditions influencing bone metabolism.

Methods: Participants were included from the National Health and Nutrition Examination Survey (NHANES), a national cross-sectional survey. BMI and WC were used separately and in combination to evaluate the presence of obesity.