A novel high-pressure low-temperature interface shear apparatus: Mechanical behavior of interface between hydrate-bearing sediment and foundation structure.

Metastable hydrates are widely distributed in shallow deep-sea sediments, posing significant risks to the long-term stability of offshore foundation structures. Unveiling the weakening mechanisms at the hydrate-bearing sediment-structure interface is crucial for establishing a theoretical framework for the safe design of deep-sea structures. A high-pressure (up to 12.

The role of LKB1 in prostate cancer: implications for tumor progression and therapy.

Liver kinase B1 (LKB1/STK11) is a key tumor suppressor that regulates cellular metabolism, epigenetic states, and multiple signaling pathways in prostate cancer (PCa). Recent studies reveal that both genetic and non-genetic LKB1 loss drives metabolic reprogramming, lineage plasticity, and treatment resistance, mainly through dysregulation of the AMP-activated protein kinase (AMPK)/mechanistic target of rapamycin (mTOR), signal transducer and activator of transcription 3 (STAT3), and Hedgehog (Hh) pathways. This review summarizes current evidence on LKB1-centered networks in PCa, highlighting the potential link between LKB1 inactivation, epigenetic remodeling, and aggressive tumor phenotypes.

WB-assisted activation of periodate by Fe(III) for decontamination: Periodate concentration-dependent mechanistic shift.

The iron-activated periodate (PI) process may be limited by the sluggish kinetics of Fe(II) regeneration and Fe(III) accumulation. Herein, tungsten boride (WB), serving as a co-catalyst, can effectively overcome the inherent drawback of oxidation reactions. The WB/Fe(III)/PI system exhibited different removal efficiencies toward sulfisoxazole (SIZ) under varying PI concentrations.

Novel paradigms in KRAS targeting: Unveiling strategies to combat drug resistance.

The Kirsten rat sarcoma viral oncogene homolog (KRAS) mutation is one of the most prevalent activating alterations in cancer. It indicates a poor overall prognosis due to its highly invasive nature. Although several KRAS inhibitors have been developed in recent years, a significant clinical challenge has emerged as a substantial proportion of patients eventually develop resistance to these therapies.

Dual-Domain Self-Consistency-Enhanced Deep Unfolding Network for accelerated MRI reconstruction.

Background And Objective: Combining iterative optimization algorithms with deep neural networks, the deep unfolding method has presented great potential for accelerated Magnetic Resonance Imaging (MRI) reconstruction. The mainstream paradigm for deep unfolding methods is to apply the data fidelity term to the reconstructed k-space and the deep regularizer to the reconstructed image. However, this paradigm has the following limitations: (1) The traditional data fidelity term corrects only the data at sampled locations in k-space, leaving the data at unsampled locations outdated, which disrupts k-space self-consistency.

Optimization of Chromosome Preparation and Karyotype Analysis of Winter Turnip Rape ( L.).

To explore the dyeing technique and karyotype analysis of winter turnip rape ( L.), the root tip of winter turnip rape Longyou 7 was used as the experimental material. Chromosome preparation technology was optimized, and karyotype analysis was carried out by changing the conditions of material collection time, pretreatment, fixation, and dissociation.

Advances in Genetic Diversity of Germplasm Resources, Origin and Evolution of Turnip Rape ( L.).

During a prolonged domestication and environmental selection, has formed diverse morphological types during a cultivation process of up to 8000 years, such as root-type turnips ( var. ), leaf-type Chinese cabbage ( var. ), oil-type rapeseed ( L.

Efficacy comparison between dentofacial orthopedic treatment and adenotonsillectomy on children with obstructive sleep apnea and skeletal class II malocclusion: a retrospective study.

Objective: This study aims to evaluate the effects of dentofacial orthopedic treatment (DOT) and adenotonsillectomy (AT) in Obstructive Sleep Apnea (OSA) children with skeletal Class II malocclusion and adenotonsillar hypertrophy (ATH), focusing on changes in sleep respiratory function, anatomic and aerodynamic characteristics of upper airway (UA).

Methods: In this retrospective study, 38 OSA children (mean age: 8.42 years, 15 females and 23 males) were included consecutively.

Whey protein meets proanthocyanidins: An ultra-fouling resistant PVDF membrane for emulsion separation.

Membrane separation technology is widely used in the treatment of oily wastewater because of its advantages such as green and high efficiency. However, after a long period of operation, the contamination resistance of conventional membranes decreases significantly and the apertures are easily clogged by oil droplets. In this study, a stable superhydrophilic micro/nano protein-polyphenol network structure was constructed on the surface of a hydrophobic PVDF membrane using whey protein (WPC) and proanthocyanidin (PC) without the addition of external particles in conjunction with the classical Cassie-Baxter model of wettability.

Organoids in the research of pancreatic neoplasms: cultivation, applications, and limitations.

Pancreatic neoplasms are mainly classified as pancreatic ductal adenocarcinoma (PDAC), pancreatic neuroendocrine neoplasms, and pancreatic cystic neoplasms. As a three-dimensional in vitro culture model, organoid technology can faithfully mimic the tissue structure, cell types, and tumor microenvironment, providing an innovative tool for pancreatic neoplasm research. This review systematically summarizes the application progress and challenges of organoid technology in pancreatic neoplasms research.

Pulsed field ablation for idiopathic premature ventricular complexes: evaluation of acute and chronic lesion characteristics with cardiac magnetic resonance imaging.

Background: To date, data about pulsed field ablation (PFA) for ventricular arrhythmias are limited, and cardiac magnetic resonance (CMR) characteristics of acute and chronic PFA lesions in the ventricles have not been described. This study sought to examine feasibility and efficacy of premature ventricular complex (PVC) ablation using focal PFA, as well as assess acute and chronic lesion characteristics using CMR.

Methods: This was a prospective, single-arm study performed at two centers in China.

[Application of China-made Toumai Robot in laparoscopic radical prostatectomy].

Objective: To evaluate the safety and efficiency of China-made Toumai Robot-assisted laparoscopic radical prostatectomy (LRP).

Methods: This study included 40 cases of PCa treated from January 2023 to May 2023 by robot-assisted LRP with preservation of the bladder neck and maximal functional urethral length, 15 cases with the assistance of Toumai Robot (the TMR group) and the other 25 with the assistance of da Vinci Robot as controls (the DVR group). We recorded the docking time, laparoscopic surgery time, vesico-urethral anastomosis time, intraoperative blood loss and postoperative urinary continence, and compared them between the two groups.

Reversed linkage-oriented intermolecular electron-transfer in isomeric covalent organic frameworks for electrochemical CO reduction.

Designing specific electroactive sites and modulating their local microenvironments of covalent organic frameworks (COFs) toward electrochemical CO reduction (ECR) have received increasing attention. However, the underlying impact of the change in intramolecular electron-transfer capability, caused by the tuning in electronic state of active sites, on the redox-mediated catalytic process still remains inadequately understood. In this work, we constructed a metalloporphyrin-based COF as the isomer of the representative COF-367-Co, with an identical chemical composition but a reversed imine-linkage orientation via Schiff-base condensation reaction using [meso-tetrakis(4-formylphenyl)porphyrin]cobalt (CoTFPP) and Benzidine (BD) as the precursors, denoted as CoTFPP-BD-COF, to exclusively investigate the linkage orientation as an individual variable for enhanced electron transmission efficiency toward ECR.

Real-world single-center data analysis of dual immunotherapy in advanced NSCLC: Efficacy, survival, and adverse events.

We collected 96 patients who received dual immunotherapy between January 2019 and June 2024, in a 1:1:1 ratio based on PD-L1 expression levels of negative (<1%), medium (1% ≤ PD-L1 ≤ 49%), and high (PD-L1 ≥ 50%). This study evaluates the efficacy and safety of dual immunotherapy in patients with advanced NSCLC, stratified by PD-L1 expression levels. Among the 96 patients, the objective response rate (ORR) was 37.

Effects of Dietary Supplementation with Fermented Leaves on Growth Performance, Meat Quality, and Sensory Traits in Sanhuang Chicken.

Incorporating specific nutritional supplements into animal diets can significantly enhance the quality and various characteristics of animal meat. This study investigated the effects of fermented leaves (ZSLs) on growth performance, meat quality, and sensory attributes in Sanhuang chickens. Three hundred one-day-old Sanhuang chickens were randomly divided into five groups and reared for 70 days: NC (control, basal diet), NF (6% unfermented ZSLs), LDG (3% fermented ZSLs), MDG (6% fermented ZSLs), and HDG (9% fermented ZSLs).

A dual-antibody sandwich immunoassay using upconversion nanoparticles and magnetic beads for fibrinogen detection.

Fibrinogen is a vital biomarker for coagulation disorders, with reduced levels increasing bleeding risk and mortality, requiring rapid detection for early diagnosis. Enzyme-linked immunosorbent assay (ELISA) is commonly employed in clinical environments; however, its prolonged processing duration and insufficient sensitivity hinder its utility for swift diagnostics. We addressed these limitations by creating a dual-antibody sandwich immunoassay that employs upconversion nanoparticles (UCNPs) and magnetic beads (MBs) for the precise and sensitive detection of fibrinogen.

Aflatoxin Contamination and Aflatoxin-producing Strain Screening in Dried Red Chili Peppers From Different Origins.

The contamination of spices with aflatoxin (AF) raises concerns regarding its health risks. In this study, 51 samples of dried red chili peppers (including 10 varieties collected from three regions) were used to investigate the AF contamination of dried red chili peppers sold in China, and the toxigenic characteristics of Aspergillus flavus strains isolated from chili peppers. AFs were identified using immunoaffinity column purification, derivatization, and high-performance liquid chromatography with fluorescence detection.

Automatically Derived Unipolar Low-Voltage-Area Burden for Predicting Atrial Fibrillation Ablation Outcomes.

Background: Unipolar voltage mapping has shown value in delineating comprehensive atrial substrates. However, the clinical application of unipolar low-voltage areas (Uni-LVAs) in atrial fibrillation (AF) ablation is limited, largely because of the absence of a well established voltage threshold. In this study we aimed to: (1) define the threshold for Uni-LVA in the left atrium (LA); and (2) investigate the association between Uni-LVA burden and ablation outcomes in a prospective AF cohort.

Preparation of aspirin inhalable powder by ultrasound-intensified anti-solvent crystallization for pulmonary drug delivery.

Aspirin is an antiplatelet agglutinating drug used clinically for the prevention and treatment of angina pectoris, myocardial infarction, and cerebral thrombosis. In this study, aspirin inhalable powder was prepared by ultrasound-intensified anti-solvent crystallization (UIAC) and developed for rapid antiplatelet aggregation, which could reduce the dose and gastrointestinal irritation. The particle size distribution, morphology, density, fluidity, and in vitro aerodynamic performance of the as-prepared powders were systematically evaluated.

Long-term outcomes after Ivor-Lewis versus McKeown esophagectomy with two-field lymphadenectomy for resectable middle or lower thoracic esophageal squamous cell carcinoma.

Purpose: This study aimed to compare long-term survival outcomes between Ivor-Lewis and McKeown esophagectomy with two-field(thoracic-abdominal) lymphadenectomy in resectable middle or lower thoracic esophageal squamous cell carcinoma.

Methods: Patients with resected middle or lower thoracic esophageal squamous cell carcinoma undergoing Ivor-Lewis or McKeown esophagectomy with two-field lymphadenectomy were enrolled and categorized into two respective groups. Propensity score matching was performed to minimize selection bias.

Design of mismatch closure for enhanced specificity in DNA strand displacement reactions.

Specific and sensitive DNA hybridization plays a key role in biotechnology, nanotechnology, and medical technology. However, traditional DNA hybridization-based strategies often require careful tuning of the binding affinity of the probe to attain a trade-off between specificity and sensitivity. Herein, we proposed energy barrier-gated dynamic selectivity to overcome this limitation.

Combined UV and Formic Acid Treatment Suppresses and Aflatoxin B on Dried Red Chili Powder.

Aflatoxin contamination poses a significant food safety risk, particularly during the storage of dried chili peppers. This study evaluated the efficacy of formic acid treatment, ultraviolet (UV) treatment, and combined UV-formic acid treatment in both preventing and controlling in dried red chili powder. Efficacy was assessed by measuring the growth diameter of colonies on un-colonized and already colonized dried red chili powder.

Musculoskeletal organoids-on-chip uncover muscle-bone communication under intermittent hypoxia.

Muscle and bone have intimate biochemical associations spatiotemporally. Yet, the muscle-bone dynamic alterations under intermittent hypoxia (IH) remain unclear, primarily due to the lack of suitable microphysiological models. Herein, we developed a novel musculoskeletal organoids-on-chip (MSK OoC), advancing an integrated study of muscle-bone biochemical communication and personalized interventional strategies.

Pulmonary delivery of excipient-free tobramycin DPIs for the treatment of lung infection with CF.

infection has become a widespread problem in patients with cystic fibrosis (CF). A safe and effective manufacturing method is required to produce antibiotic dry powder inhalations (DPIs) which can be effectively delivered to treat lung infections. In this study, an excipient-free tobramycin inhalable powder was prepared using spray freeze-drying (SFD) method.

Research on the mechanical properties of marine soft clay solidified with waste tile powder-calcium carbide residue geopolymer under freeze-thaw cycles.

Based on the demand of marine soft clay treatment, it is still urgent to seek green cementitious materials. Meanwhile, knowledge gaps exist regarding the application of waste tile powder (WTP)-calcium carbide residue (CCR) geopolymer into marine soft clay. This research explores the mechanical properties and freeze-thaw durability of WTP-clay-CCR system.