Implementation of Early Mobilization in Post-CABG Patients: A Constructivist Grounded Theory Study.

Purpose: Coronary artery bypass grafting (CABG) is an essential intervention for treating coronary artery disease. However, the effectiveness of postoperative rehabilitation often depends on the implementation of early mobilization by patients. In practice, adherence to early mobilization post-CABG is frequently suboptimal.

Engineered Mesenchymal Stem Cell-Derived Small Extracellular Vesicles Mitigate Liver Fibrosis by Delivering USP10 to Reprogram Macrophage Phenotype.

The utilization of mesenchymal stem cells (MSCs) serves as an encouraging strategy for treating liver fibrosis. However, precise mechanisms are not completely understood. Recently, small extracellular vesicles (sEVs) have emerged as major paracrine effectors mediating the anti-fibrotic effects of MSCs.

Analytical and clinical evaluation of an automated high-sensitivity cardiac troponin I assay for whole blood.

Objectives: To evaluate the performance of the iStar high-sensitivity cardiac troponin I (hs-cTnI) assay, focusing on its sensitivity, precision, linearity, and consistency with plasma samples, and to establish sex-specific 99th percentile upper reference limits.

Methods: The iStar hs-cTnI assay was assessed using the Drawray iStar 500 analyzer. Key performance metrics such as the limits of blank (LoB), detection (LoD) and quantitation (LoQ), precision, linearity, and agreement between sample types were evaluated according to Clinical and Laboratory Standards Institute (CLSI) guidelines.

Association between serum osmolality and the risk of ventilator-associated pneumonia in patients undergoing prolonged mechanical ventilation: a retrospective cohort study from MIMIC-IV database.

Background: The study aimed to investigate the relationship between serum osmolality with the risk of ventilator-associated pneumonia (VAP) and in-hospital mortality in patients with prolonged mechanical ventilation (PMV).

Method: This study was a retrospective cohort study with data derived from the Medical Information Mart for Intensive Care database. Patients were categorized into two groups based on a serum osmolality cutoff of 295 mmol/L, with the reference group having values below 295 mmol/L.

A Novel Adaptive Flexible Capacitive Sensor for Accurate Intravenous Fluid Monitoring in Clinical Settings.

Intravenous infusion is an important clinical medical intervention, and its safety is critical to patient recovery. To mitigate the elevated risk of complications (e.g.

High allostatic load in women in the third trimester associated with delayed onset of lactogenesis: A prospective study.

Problem: Stress is an important factor affecting postpartum lactation and is associated with the delayed onset of lactogenesis. Allostatic load is a comprehensive index for measuring chronic stress that effectively reflects the degree of chronic stress in pregnant women. However, no studies have explored the relationship between allostatic load and delayed onset of lactogenesis in pregnant women.

Evaluation of MC-80 automatic blood cell morphology analyzer in identifying the morphology of blood cells in patients with hematological diseases and normal samples.

Background: This study evaluates the performance of the MC-80 automatic blood cell morphology analyzer in identifying the morphology of blood cells in patients with hematological diseases and normal samples.

Methods: A total of 320 patients with hematological diseases and 200 healthy persons with health examination from the health examination center between January 2023 and May 2024 were selected as research subjects, and 520 venous blood samples were collected in this study. The morphology of white blood cells, nucleated red blood cells, platelets, and mature red blood cells was all detected by the MC-80 automatic blood cell morphology analyzer and compared with the manual microscopic examination.

A General and Interpretable Adatom Model for Classifying Surface Morphologies of Nonmetallic Elements on Metal Substrates.

We present a general and interpretable adatom model that enables the prediction and understanding of stable surface morphologies of nonmetallic elements deposited on metal substrates. By calculating the formation energies of isolated adatoms on various metal surfaces, we reveal the competition between interfacial interactions and the self-aggregation tendencies of the deposited elements. Based on this model, we classify four distinct surface morphologies that arise from nonmetal-metal substrate combinations.

Unveiling Hidden Health Risks: Machine Learning Enhanced Modeling of Plastic Additive Release Kinetics in Fresh Produce Packaging.

Fresh produce packaging (FPP) plays a critical role in protecting fruits and vegetables from various environmental factors. However, the presence, migration, and human health risks of additives in FPP have received limited attention. This study investigated 73 commonly used additives across six categories of FPP samples collected in China.

A renewable enzyme-driven dissipative DNA strand displacement strategy for T4 polynucleotide kinase activity assay and inhibitor screening.

Development of dissipation-based sensing systems would provide enormous opportunities for reliable bioanalysis owing to their oscillatory signal response pattern. Herein, a fluorescence biosensor was developed for the detection of T4 polynucleotide kinase (T4 PNK) activity and inhibitor screening, leveraging an enzyme-driven dissipative strand displacement reaction. The sensing system involves the T4 PNK-catalyzed phosphorylation of a fuel strand and then a dissipative strand displacement reaction facilitated by a fuel consumption unit, lambda exonuclease (λ-Exo).

ARID1A deficiency attenuates the response to EGFR-TKI treatment in lung adenocarcinoma.

Background: Concurrent genetic alterations (e.g., TP53 comutations) significantly impair EGFR-TKI responsiveness and survival outcomes in EGFR-mutant lung adenocarcinoma (LUAD).

Unraveling the role of M2 TAMs in ovarian cancer dynamics: a systematic review.

Tumor-associated macrophages (TAMs) represent the dominant immune cell subset within the ovarian tumor microenvironment, which exhibits remarkable plasticity. They can readily undergo polarization toward an immunosuppressive M2-like phenotype in response to various factors secreted by tumor cells, playing a pivotal role in ovarian cancer advancement and the development of resistance to chemotherapy. M2 TAMs promote the invasiveness of ovarian cancer cells and their resistance to therapeutic agents through the secretion of numerous tumor-promoting factors, including cytokines, chemokines, enzymes, and exosomes.

Attapulgite-Assisted In Situ Anchoring of Ultrasmall Ag Nanoparticles for Enhanced Eradication of Multidrug-Resistant Bacterial Biofilms and Accelerated Wound Healing.

Silver nanoparticles (Ag NPs) have emerged as a promising solution to combat biofilm-related infections caused by multidrug-resistant bacteria. However, their practical application remains limited due to their tendency to aggregate and exhibit high toxicity at elevated concentrations. Here, we developed a peel water extract-mediated hydrothermal process to facilitate the heterogeneous nucleation of Ag NPs on attapulgite (APT) nanorods and prepared Ag/APT nanocomposites with ultrasmall Ag NPs (<2 nm) uniformly anchored on APT nanorods.

Optimizing surgical precision: a comparative study of three-port vs. four-port robotic-assisted lobectomy for NSCLC.

Background: In recent years, robot-assisted thoracoscopic surgery has seen major advances. The feasibility and safety of this new surgical procedure have been widely recognized. However, only a few studies have investigated the short-term postoperative outcomes of lobectomy in early-stage non-small cell lung cancer (NSCLC) patients using different numbers of ports in Da Vinci robot-assisted surgery.

Single-Particle Collision Electrochemical Biosensor Developed by a Typical Alkaline Phosphatase-Catalyzed Silver Deposition Reaction.

Considering the intrinsic importance of alkaline phosphatase (ALP) as a biomarker in disease monitoring and as a mostly widely used biolabel for signal transmission in bioanalysis, the development of a new ALP assay method is highly pursued. Herein, a well-known ALP-catalyzed silver deposition reaction onto gold nanoparticles (Au NPs) was developed into a single-particle-collision-based electrochemical biosensor. ALP-catalyzed dephosphorylation of ascorbic acid 2-phosphate (AA-P) resulted in ascorbic acid (AA), which in turn reduced the silver ion to form a silver nanoshell on the surface of Au NPs (Au@Ag NPs).

Combination therapy with Chicoric acid and PD-1/PD-L1 blockade improves the immunotherapy response in patient-derived ovarian cancer xenograft model.

Purpose: Limited treatment options exist for refractory ovarian cancer (OC) due to its poor response to immune therapies. Therefore, there is an urgent need to develop new effective treatment strategies. Chicoric acid (CA) is reported to have immune-enhancing properties, but its efficacy in cancer treatment is not well understood.

Host FSTL1 defines the impact of stem cell therapy on liver fibrosis by potentiating the early recruitment of inflammatory macrophages.

Adult stem cell therapy holds great promise for treating decompensated liver cirrhosis on the basis of animal studies, despite uncertainty about its clinical therapeutic efficacy and unclear underlying mechanisms. Here, we investigated the role of follistatin-like 1 (FSTL1), a profibrotic and proinflammatory matricellular protein, in inflammation-related heterogeneity in stem cell therapy. Our results showed that a high level of circulating FSTL1 is significantly correlated with therapeutic response in patients with cirrhosis.

A study of the purchase intention of alternative foods.

Environmental pollution has drawn widespread attention to food safety. People are becoming more conscious of their health and the sustainable development of food, and are setting higher standards for food safety, which have led to the development of better alternatives to food. In this study, we examine the willingness of college students to buy alternative foods and the factors that influence their purchase decisions, as well as the sustainable development of alternative products.

MMP19 in vascular smooth muscle cells protects against thoracic aortic aneurysm and dissection via the MMP19/Aggrecan/Wnt/β-catenin axis.

Background: Thoracic aortic aneurysm and dissection (TAAD) is a life-threatening cardiovascular event characterized by high mortality rates. Previous studies have shown that matrix metalloproteinases 19 (MMP19) was involved in TAAD formation, while the detailed role of MMP19 in TAAD pathogenesis and underlying mechanism remain unclear.

Methods: To investigate the role of MMP19 in the progression of TAAD, we generated global Mmp19 knockout mice, as well as VSMCs (vascular smooth muscle cells)-specific Mmp19 knockdown mice, and established a BAPN-induced TAAD model.

Role of the FOXM1/CMA/ER stress axis in regulating the progression of nonalcoholic steatohepatitis.

Background/aims: The molecular mechanisms driving nonalcoholic steatohepatitis (NASH) progression are poorly understood. This research examines the involvement of chaperone-mediated autophagy (CMA) in NASH progression.

Methods: Hepatic CMA activity was analysed in NASH mice and patients.

Resilience and vulnerability of post-ostomy patients with early-onset colorectal cancer from the perspective of social-ecological theory: a qualitative study.

Background: The incidence rate of colorectal cancer (CRC) is rising among adults under the age of 50 (early-onset colorectal cancer, EOCRC). Post-ostomy dysfunction, along with negative perceptions due to incorrect public views and a decline in quality of life, has a significant impact on these individuals, their families, and social relationships.

Objective: By understanding the adaptation process of post-ostomy EOCRC(POEOCRC) patients, this study aims to provide information for developing targeted nursing interventions for this population.

Integrative analysis of ferroptosis in the hypoxic microenvironment of gastric cancer unveils the immune landscape and personalized therapeutic strategies.

Background: Ferroptosis is a cell death mode caused by excessive accumulation of lipid peroxides caused by disturbance of intracellular metabolic pathway, which is closely related to iron and cholesterol metabolism homeostasis. Its regulation within the hypoxic metabolic tumor microenvironment (TME) has the potential to improve the effectiveness of tumor immunotherapy. The predictive role of ferroptosis in gastric cancer (GC) hypoxia TME, particularly in relation to TME immune cell infiltration, has not been fully explained.

Risk Factor Analysis and Molecular Epidemiological Investigation of Carbapenem-Resistant (CRE) Infection in Patients with Acute Pancreatitis.

Objective: Patients with acute pancreatitis (AP) complicated by carbapenem-resistant (CRE) infection often have a higher mortality rate. However, little investigation on the risk factor analysis has been published for the AP complicated by CRE. Therefore, this study conducted a retrospective analysis of the clinical characteristics, risk factors, and molecular epidemiological features associated with CRE infection in patients with AP.

Dexmedetomidine regulates the anti-oxidation and autophagy of adipose-derived stromal cells under HO-induced oxidative stress through Nrf2/p62 pathway and improves the retention rate of autologous fat transplantation.

To investigate the protective mechanism of dexmedetomidine (DEX) on adipose-derived stromal cells (ADSCs) under oxidative stress model and its promotion effect on the retention rate of adipose granule transplantation by and experiments. The experiment was divided into control group, model group (ADSCs + HO+normal serum), DEX group (ADSCs + H0+DEX drug-containing serum), autophagy agonist group (ADSCs + HO+rapamycin (RAP)+normal serum), RAP + DEX group (ADSCs + HO+normal serum), RAP + DEX drug-containing serum), autophagy inhibitor group (ADSCs + HO+chloroquine (CQ)+normal serum), CQ + DEX group (ADSCs + HO+CQ + DEX drug-containing serum). HO-1, GSH-PX, SOD and CAT in ADSCs under oxidative stress model were measured.