A Benchmark of Semi-Supervised scRNA-seq Integration Methods in Real-World Scenarios.

Semi-supervised methods for single-cell RNA-seq integration promise to improve batch correction and biological signal preservation by leveraging cell-type labels. However, their reported benefits often rely on overly idealized settings. Here, we present the first systematic benchmark of five leading semi-supervised methods (scANVI, scGEN, ssSTACAS, scDREAMER, ItClust) against five widely used unsupervised baselines across six diverse datasets.

Research progress on multisensory stimulation therapy for pain during retinopathy of prematurity screening.

Retinopathy of prematurity (ROP) is a retinal disease characterized by abnormal vascular proliferation, primarily associated with premature delivery and low birth weight. Advances in perinatal and neonatal care have increased survival rates but have also contributed to a rising incidence of ROP, necessitating regular ROP screening. However, the screening procedure, which involves an eyelid speculum and ophthalmoscope, frequently induces pain.

Zeaxanthin augments CD8 effector T cell function and immunotherapy efficacy.

The detailed mechanisms underlying the regulatory significance of dietary components in modulating anti-tumor immunity remain largely unknown. Here, we apply a co-culture-based screening approach using a blood nutrient compound library and identify zeaxanthin (ZEA), a dietary carotenoid pigment found in many fruits and vegetables and known for its role in eye health, as an immunomodulator that enhances the cytotoxicity of CD8 T cells against tumor cells. Oral supplementation with ZEA, but not its structural isomer lutein (LUT), enhances anti-tumor immunity in vivo.

Emergency surgical intervention for bulbar-cervical spinal cord hemorrhage: a case report and review of management strategies.

Background And Importance: Spinal cord hemorrhage (SCH), particularly involving the bulbar-cervical segment (medulla oblongata to C7), is a rare and life-threatening neurological emergency. Due to its anatomical proximity to respiratory, motor, and sensory centers, it often leads to catastrophic neurological deficits. Etiologies include vascular malformations, coagulopathy, or idiopathic causes, yet its low incidence (<1%) poses significant challenges in early diagnosis and management.

The application of silent MRA in follow-up after intracranial aneurysm endovascular treatment.

PurposeTo assess the diagnostic value of silent MRA in the follow-up of intracranial aneurysms (IA) post-endovascular treatment.MethodsFrom March 2023 to March 2024, we retrospectively collected data on patients with IA who underwent endovascular intervention and received silent MRA follow-up. All images were anonymized and evaluated on a 5-point scale: 1 (not visible, strong artifacts); 2 (poor quality, numerous artifacts/blurring); 3 (acceptable quality, moderate artifacts/blurring); 4 (good quality, minor blur artifacts); 5 (very good quality, nearly equal to DSA).

Bisulfite Sequencing to Map 5-Methylcytosine Sites in Human Respiratory Syncytial Virus RNA.

Viral epigenetic modification is an emerging field. As an obligate intracellular parasite, viral genetic material is exposed to host RNA-modifying enzymes. Currently, more than 180 types of RNA modifications have been identified.

Occurrence of fatal vertebrobasilar dolichoectasia after occlusion of unilateral internal carotid artery.

Background: Vertebrobasilar dolichoectasia (VBD) is a rare cerebrovascular disorder. The natural history of patients with VBD is generally poor, and current treatments do not appear to provide significant clinical benefit. Therefore, understanding the underlying mechanisms of VBD and implementing appropriate strategies in advance are particularly important.

FTO degrader impairs ribosome biogenesis and protein translation in acute myeloid leukemia.

Targeting ribosome biogenesis and protein translation has emerged as a promising avenue for cancer therapy. The fat mass and obesity-associated protein (FTO), an RNA -methyladenosine (mA) eraser, has been identified as an oncogenic factor in acute myeloid leukemia (AML). Here, we present the development of an FTO degrader that selectively degrades FTO in AML cells, demonstrating superior efficacy both in vitro and in vivo.

An Anti-Range-Deception-Jamming Method for Networked Moving Radar Based on Trajectory Optimization.

Aiming at the problem that the anti-range-deception-jamming effect of a networked moving radar system is severely affected by the spatial distribution of each radar, an anti-range-deception-jamming method for networked moving radar based on trajectory optimization is proposed. Firstly, the anti-jamming method of networked moving radar considering the radar position error (RPE) is proposed. Then, the theoretical expression for the false target (FT) misjudgment probability of networked moving radar is deduced.

In situ proximity labeling of proteins associated with circRNA and RNA G-quadruplexes.

Profiling the protein interactome of specific RNA loci aids in understanding the molecular mechanisms of regulatory RNA. However, current RNA-centric methods have sufficient space for improvement in terms of efficiency and biocompatibility. Here we developed TurboID-assisted proximity labeling of targeted RNA-interacting proteins (TAPRIP), in which the proximity labeling enzyme miniTurbo is attached to an RNA-targeting element, CIRTS3, to label the targeted RNA-interacting proteins, which are then analyzed by mass spectrometry.

ALKBH1 drives tumorigenesis and drug resistance via tRNA decoding reprogramming and codon-biased translation.

Cancer cells utilize codon-biased translation to fuel tumorigenesis and drug resistance, but underlying mechanisms remain poorly understood. Here, we show ALKBH1 is overexpressed in acute myeloid leukemia (AML) and essential for leukemia stem/initiating cell (LSC/LIC) self-renewal and AML development/maintenance, whereas dispensable for normal hematopoiesis. ALKBH1 enhances mitochondrial assembly/function and oxidative phosphorylation (OXPHOS), crucial for AML survival/proliferation and resistance to venetoclax, a potent BCL2 inhibitor and widely-used first-line targeted therapy for AML in clinic.

Advanced Ensemble Staking Model Employing cfDNA Fragmentation for Early Detection of Esophageal and Gastric Cancer.

Esophageal and gastric cancers are aggressive malignancies with poor prognoses due to late-stage diagnosis. Our study recruited 275 healthy participants, 201 gastric cancer patients, 74 esophageal patients and 103 patients with precancerous conditions. The participants were assigned into training and validation cohorts.

An innovative fungal-specific targeted next-generation sequencing method: analytical performance and a single-center prospective clinical study.

Background: Invasive pulmonary fungal infections (IPFIs) pose significant diagnostic challenges, particularly in immunocompromised patients. Accurate and timely diagnosis is crucial to improve outcomes. While metagenomic next-generation sequencing (mNGS) is widely utilized, it is expensive and affected by host DNA interference.

Nanograin-Twin-Nanograin Alternating Composite Structure Enable Improved Cross-Interface Cu─Cu Bonding at Low Thermal Budgets.

Chip stacking using through-silicon via (TSV) and direct copper-to-copper (Cu─Cu) bonding technology has emerged as a superior solution to overcome the limitations of Moore's law. However, conventional approaches face a fundamental trade-off: coarse-grained Cu requires high bonding temperatures (>300 °C), while nanograined Cu is unstable and tends to coarsen even at room temperature after electroplating. Here, this paradigm is broken through a unique composite copper (comp-Cu) architecture featuring alternating nanograin (ng─Cu) and (111)-oriented nanotwin (nt─Cu) domains.

5-hydroxymethylcytosine deposition mediates Polycomb Repressive Complex 2 function in -amplified neuroblastoma.

-amplification is a strong predictor of poor prognosis in neuroblastoma, an embryonal malignancy that accounts for 15% of pediatric cancer deaths. Here, we found that -amplified neuroblastoma tumors had increased 5-hydroxymethylcytosine (5-hmC) deposition on Polycomb Repressive Complex 2 (PRC2) target genes. 5-hmC and H3K27me3, a catalytic product of PRC2, directly co-localized at the nucleosomal level in -amplified neuroblastoma.

Single-nucleotide Resolution Epitranscriptomic Profiling Uncovers Dynamic mA Regulation in Bovine Preimplantation Development.

RNA -methyladenosine (mA) plays a crucial role in regulating gene expression during early embryonic development. However, the mA dynamics at single-nucleotide resolution in preimplantation development remain uncharacterized, and the functional significance of site specific mA modifications in key developmental regulators is largely unknown. Here, using SAC-seq, a single-base resolution, antibody-independent mA profiling method, we generate the first comprehensive mA landscape in bovine oocytes and preimplantation embryos.

5-azacytosine induces cytotoxicity via 5-methylcytosine depletion on chromatin-associated RNA in leukemia.

Unlabelled: 5-azacytidine (5-azaC) is a DNA hypomethylating agent clinically used to improve outcomes in myeloid malignancies. However, 5-azaC treatment causes gene dysregulation inconsistent with DNA hypomethylation changes, suggesting alternative mechanisms of action by 5-azaC. As a ribonucleoside analogue, 5-azaC is more readily incorporated into nascent RNA.

Non-measured and DVH-based patient-specific QA framework for lung SBRT VMAT with machine learning integration.

Background: Conventional patient-specific quality assurance (PSQA) methods rely on time-consuming physical measurements. While previous studies have successfully employed machine learning (ML) models to predict gamma passing rates (GPRs), their clinical utility remains limited due to GPR's weak correlation with dose-volume histogram (DVH) parameters. Thus, developing a novel PSQA framework that is non-measured and DVH-based (NMDB) presents a promising alternative.

DCPS modulates TDP-43 mediated neurodegeneration through P-body regulation.

The proteinopathy of the RNA-binding protein TDP-43, characterized by nuclear clearance and cytoplasmic inclusion, is a hallmark of multiple neurodegenerative diseases, including amyotrophic lateral sclerosis (ALS), frontotemporal dementia (FTD), and Alzheimer's disease (AD). Through CRISPR interference (CRISPRi) screening in human neurons, we identified the decapping enzyme scavenger (DCPS) as a novel genetic modifier of TDP-43 loss-of-function (LOF)-mediated neurotoxicity. Our findings reveal that TDP-43 LOF leads to aberrant mRNA degradation, via disrupting the properties and function of processing bodies (P-bodies).

A noninvasive and highly efficient epigenetic predictive model for efficacy of the GOLP regimen in patients with intrahepatic cholangiocarcinoma.

The GOLP regimen (Gemcitabine, Oxaliplatin, Lenvatinib and anti-PD1 antibody) has been a promising first-line treatment for advanced intrahepatic cholangiocarcinoma (iCCA). A noninvasive tool to predict the response to GOLP regimen is needed for clinical practice. In this study, 188 cell-free DNA samples were collected before each cycle and after the third cycle (P0 to P3) from 47 iCCA patients receiving GOLP regimen.

Metastable Nanocrystalline Copper for Effective Copper-to-Copper Bonding.

Direct copper-to-copper (Cu-Cu) bonding is critical for advanced electronic packaging. However, high bonding temperatures and prolonged bonding times present challenges for heterogeneous integration. To lower the thermal budget, researchers explored grain engineering with nanotwinned Cu (NT-Cu) and nanocrystalline Cu (NC-Cu).

Perioperative management of total hip arthroplasty in hemophilia A following gene therapy.

Not available.

Modifications of microbiome-derived cell-free RNA in plasma discriminates colorectal cancer samples.

Circulating cell-free RNA (cfRNA) in plasma represents a promising avenue for cancer detection. We report low-input multiple methylation sequencing, a method for profiling modification patterns in cfRNA, enabling the detection of diverse transfer RNAs and small noncoding RNAs derived from both the human genome and the microbiome. RNA modification patterns in microbiome-derived cfRNA accurately reflect host microbiota activity and hold potential for the early detection of colorectal cancer.