Screening efficacy of erythroferrone and hepcidin in identifying thalassemia with iron-deficiency anemia among pregnant women.

This study investigated the prevalence of Thalassemia (Thal) gene in pregnant women in the Quanzhou area, China. And explored the clinical application value of Erythroferrone (ERFE) and Hepcidin in screening pregnant women with Thal complicated by Iron-deficiency anemia (IDA, defined as serum ferritin (SF) < 20 μg/L). From January 2020 to December 2022, the detection rate of Thal in suspected Thal populations was 35.

Daptomycin-Loaded Nano-Drug Delivery System Based on Biomimetic Cell Membrane Coating Technology: Preparation, Characterization, and Evaluation.

Staphylococcus aureus () is a clinically significant pathogenic bacterium. Daptomycin (DAP) is a cyclic lipopeptide antibiotic used to treat infections caused by multidrug-resistant Gram-positive bacteria, including . However, DAP currently faces clinical limitations due to its short half-life, toxic side effects, and increasingly severe drug resistance issues.

Chrysin enhances sunitinib sensitivity in renal cell carcinoma by inducing ferroptosis via targeting PI3K/Akt/GPX4 pathway.

Renal cell carcinoma (RCC) continues to pose a significant clinical challenge due to its high resistance to conventional therapies. Sunitinib, a first-line treatment for metastatic RCC, is often limited by acquired resistance, necessitating novel therapeutic strategies. Chrysin, a natural flavonoid with known anticancer properties, has shown potential in various malignancies; however, its role in RCC is still not well understood.

Pseudomonas fluorescens P34 colonization impacts expression changes in wheat roots, reshapes rhizosphere microbial communities and promotes wheat plant growth.

Plant growth-promoting rhizobacteria (PGPR) can stimulate crop growth and performance through multiple mechanisms, making them promising bioinoculants for sustainable agriculture. Among known PGPR species, Pseudomonas fluorescens has attracted considerable attention because of its superior growth-promoting mechanisms and broad adaptability. Although P.

SOD1 is delivered to lysosomes via autophagy to maintain lysosomal function and integrity.

The gene encoding superoxide dismutase 1 (SOD1) is often mutated in familial amyotrophic lateral sclerosis (ALS), affecting motor neurons. Compared with ALS-associated mutant SOD1, the function of WT SOD1 is less explored. We demonstrate that during starvation, WT and mutant SOD1 are transported into lysosomes.

Phenanthrene exposure disrupts puberty onset in SD rats by influencing hypothalamic neural networks.

Polycyclic aromatic hydrocarbons (PAHs) are gaining attention due to their widespread occurrence and serious health risk. Epidemiological survey research discovered that PAHs are associated with compromised pubertal development. To investigate this, weaned rats were administrated to corn oil (control group) or phenanthrene (PHE) for 21 days.

Spinning Top-like Nanoprobes for Direct Visualization of Cooperative Ca-Binding-Induced Conformational Switching in Single Calmodulin Molecules.

Understanding the rapid, domain-specific conformational dynamics of single calmodulin (CaM) molecules remains a major challenge due to the limited temporal resolution of existing single-molecule techniques. Here, we present a mechano-responsive strategy using high-speed nanoscale spinning tops (NSTs) as nanoprobes to directly resolve these dynamics. By constructing a single-molecule CaM protrusion on a protein corona-coated gold nanorod, we enabled stochastic thermally driven rotation, whose speed is sensitively modulated by conformational changes in CaM.

The association between psychosocial stress trajectories and emotional and behavioral problems in children and adolescents.

Adolescence is a sensitive period with emotional and behavioral problems, and childhood long psychosocial stress exposure might relate with these problems. This study aimed to identify the overall trajectory and multi-trajectory of psychosocial stress and relate these to emotional and behavioral problems in adolescence. The population were from a pubertal development cohort which was established in Chongqing, Southwest China in 2014.

Identification of Mycobacterium Species by DNA Microarray Chip Method.

This study presents a DNA microarray chip method designed for the accurate identification of Mycobacterium species. By leveraging asymmetric PCR amplification and hybridization principles, this method targets 17 common mycobacteria, including those in the Mycobacterium tuberculosis complex and various non-tuberculous mycobacteria. It is applicable to a wide range of clinical specimens like sputum, pus, bronchoalveolar lavage fluid, cerebrospinal fluid, and puncture fluid from patients suspected of mycobacterial diseases.

Thin-Layer Covalent Organic Framework-Coated Gold Superstructures for Label-Free Raman Spectroscopic Monitoring of Multiple Volatile Organic Compounds.

The detection of multiple volatile organic compounds (VOCs) encountered challenges such as sample complexity, signal reproducibility, sensitivity limitations, and interference resistance. Here, we prepared thin-layer COF-coated gold superstructures (GSP@COF) to capture and identify the fingerprint spectrum of the VOCs. The GSP@COF substrate ensured an even distribution of SERS hotspots, boosted signal intensity, and prevented nanoparticle aggregation, resulting in consistent and reliable analytical results, with less than 5% spatial variations in signal intensity.

Self-assembled transdermal nanogels control scar formation by inhibiting fibroblast proliferation and fibrosis with glycolysis regulation via the PI3K/Akt/mTOR pathway.

Hypertrophic scar (HS) is one of the most common challenges in the field of plastic and reconstructive surgery. HS formation is associated with the abnormal activation of fibroblasts. These fibroblasts exhibit excessive proliferative and fibrotic behavior, which can be induced by glycolysis dysregulation.

Machine learning molecular dynamics reveals atomistic mechanisms of ion beam damage in calcium fluoride.

Calcium fluoride (CaF) crystals are regarded as ideal materials for optical components due to their excellent ultraviolet (UV) transmittance and optical anisotropy. High-precision and low-damage CaF crystals can be prepared by ion beam processing, but the atomic-scale surface and interfacial stability under ion beam processing remains poorly understood. In this paper, a machine learning potential (DP) based on the DeePMD method was developed to explore the underlying physical mechanisms of microstructure and property changes in calcium fluoride under ion beam bombardment.

Microgravity-Induced Cognitive Decline: Investigating the Pathogenic Mechanisms of RyR2 Hyperphosphorylation and S107 Intervention.

Cognitive function is crucial for astronauts to successfully complete space missions. Therefore, investigating the mechanisms by which one of the primary stressors in the space environment-microgravity-affects cognitive function is of great significance. Although synaptic plasticity is recognized as being highly associated with cognitive function, the mechanisms underlying its changes under microgravity remain unclear.

Highly Specific and Fast-Responsive Semicarbazide-Sensitive Amine Oxidase Nanoprobe for the Serological Diagnosis of Parkinson's Patients.

Semicarbazide-sensitive amine oxidase (SSAO), a multifunctional enzyme associated with various diseases, including neurodegenerative disorders, has emerged as a potential biomarker for Parkinson's disease (PD). However, current SSAO detection methods, such as immunoassays and mass spectrometry, are limited by their complexity, nonselectivity, high cost, and the use of hazardous radioactive materials. In this study, we developed a highly specific, sensitive, and cost-effective method for SSAO activity detection based on optical gold nanoparticles (AuNPs).

Natural Compounds in Cancer Therapy: Revealing the Role of Flavonoids in Renal Cell Carcinoma Treatment.

Renal cell carcinoma (RCC) is the most lethal malignancy of the urinary system, with limited treatment options due to drug resistance and the adverse effects associated with current therapies. This review aims to systematically examine the therapeutic potential of flavonoids, which are natural polyphenolic compounds possessing anti-inflammatory, antioxidant, and anticancer properties, in the context of RCC treatment. We summarize the anticancer activities of 26 natural flavonoids, classified into six subclasses, and explore their mechanisms of action, including the inhibition of tumor cell proliferation, migration, and invasion, as well as the induction of apoptosis, autophagy, and ferroptosis.

A novel peptide TCL6148 induces ferroptosis via the GOT1/GPX4 pathway to enhance sunitinib sensitivity in renal cell carcinoma.

Renal cell carcinoma (RCC) represents a deadly type of genitourinary cancer, noted for its strong tendency to metastasize and its unfavorable outlook for patients. Despite significant advancements in targeted therapies and immunotherapies, tumor heterogeneity and therapeutic resistance remain formidable challenges, underscoring the urgent need for novel treatment strategies. Long non-coding RNAs (lncRNAs), once thought to be non-translatable, have recently been discovered to encode functional peptides that are crucial in the progression of tumors.

Unraveling the mechanisms of tricetin in renal cell carcinoma treatment through network pharmacology and experimental validation.

Renal cell carcinoma (RCC), one of the most common types of kidney cancer, represents a major global health concern due to its increasing incidence and high death rates. While conventional treatment modalities have improved patient outcomes, they often face limitations such as drug resistance, severe adverse effects, and limited efficacy in advanced or metastatic cases. These challenges highlight the urgent need for novel therapeutic approaches to enhance RCC management.

PVDF/PANi@HNT Nanocomposite Membranes: Pioneering Solutions for Piezoelectric Sensing and Energy Harvesting Efficiency.

Piezoelectric nanogenerators (PENGs) based on electrospun membranes hold significant promise for applications in wearable electronics, biomedicine, and multifunction sensors. The evolution of composite fiber membranes can effectively address the challenges of low output power and poor stability in PENGs. Herein, a composite structured polyaniline-coated halloysite nanotube (PANi@HNT) was synthesized through oxidative polymerization.

Pre-Label-Free Three-Dimensional Imaging of Cellular Bottom Topography with a Charge-Lock Electrochemiluminescence Microscope.

Cellular bottom structure plays a vital role in cell mobility, but its narrow space makes it difficult to study. Currently, it remains challenging to panoramically map the cellular bottom topography variations due to the potential cellular damage and off-target effects of luminophores when using current ultra-high-resolution optical microscopy. Here, we present charge-lock spatiotemporal transformation electrochemiluminescence (CL-STTECL) microscopy.

Challenging the Safety Threshold: Neurotoxicity in Bipolar Disorder Treatment with Lithium at Therapeutic Serum Levels.

Bipolar disorder is a complex mental disorder that often requires long-term medication management. Lithium carbonate is widely used to prevent and treat the recurrence of bipolar disorder. However, even with normal serum lithium levels, some rare but serious side effects may occur.

Differential diagnosis of clear cell renal cell carcinoma with low signal intensity on T2WI from angiomyolipoma without visible fat on MR imaging.

Purpose: This study aimed to determine the potential of magnetic resonance imaging (MRI) parameters in differentiating between angiomyolipoma without visible fat (AML.wovf) and clear cell renal cell carcinoma (ccRCC) with low signal intensity on T2-weighted imaging (T2WI).

Materials And Methods: This is a retrospective study involving 36 cases of ccRCC and 17 cases of AML.

A Hypoxia-Triggered Bioreduction of Hydrophilic Type I Photosensitizer for Switchable In Vivo Photoacoustic Imaging and High-Specificity Cancer Phototherapy.

Considering that hypoxia is strongly connected with tumor proliferation, metastasis, invasion, and drug resistance, it is of significant implication for alleviating the effects of hypoxia in tumor treatment. The negligible oxygen-dependent nature of type I photosensitizers (PSs) has made them appropriate candidates for the treatment of hypoxic tumors. However, the lack of effective molecular design approaches, the phototoxicity of PSs to normal tissue before and after treatment, and the drawbacks of poor hydrophilicity severely hinder the development of PSs in hypoxic tumor therapy.

2-Undecanone induces ferroptosis via the STAT3/GPX4 pathway to enhance sensitivity of renal cell carcinoma to sunitinib.

The development of resistance significantly reduces the efficacy of targeted therapies, such as sunitinib, in renal cell carcinoma (RCC) patients, emphasizing the need for novel therapeutic agents. Natural products, known for their diverse chemical structures and mechanisms of action, offer promising anti-tumor potential with favorable safety profiles and lower toxicity compared to synthetic drugs. 2-Undecanone, a natural compound extracted from Houttuynia cordata Thunb.

DNA Nanotubule-Based Nanodevices with ATP-Responsive Gating for Direct Cytosolic Delivery of Nucleic Acids and Proteins.

Delivering biomacromolecules to the cytosol remains a formidable challenge, as these molecules are predominantly sequestered within endosomes after endocytosis. The limited efficacy of current delivery systems in promoting reliable endosomal escape underscores the need for innovative strategies. Here, we report a DNA origami nanotubule to construct transmembrane delivery nanodevices with size-selective gating and ATP-responsive channel activation.

The effect of radio-frequency ablation in treating pulmonary ground glass nodule patients and its influence on pulmonary function.

Background: With widespread application of low-dose thin-section chest CT screening, the detection rate of patients with subsolid nodules (SNs) as CT findings has increased remarkably.

Objective: To clarify effect of radio-frequency ablation (RFA) in treating pulmonary ground glass nodule (GGN) patients and its impact on pulmonary function.

Methods: A total of 100 patients diagnosed with pulmonary GGN in our hospital from January 2019 to December 2021 by pulmonary thin-section CT + enhanced imaging examination and all underwent simultaneous needle biopsy were enrolled and randomly divided into control group and observation group, with 50 cases each.