Revealing the Ion Channel Switch of the Cell Membrane via Electrochemiluminescence Pattern Transformation.

Ion channels as transmembrane proteins provide an important transport pathway for specific ions through cell membranes, which are crucial to studies involving the pathophysiology of the nervous system. However, the switch between different conformational states (i.e.

RIPK4-mediated MFN2 degradation drives osteogenesis through mitochondrial fragmentation and restricts myelopoiesis by blocking mitochondrial transfer.

Human RIPK4 mutation leads to Bartsocas-Papas syndrome (BPS), characterized by severe skin, craniofacial and limb abnormalities. Currently, our understanding of RIPK4's function has focused on epidermal differentiation and development, whether RIPK4 regulates skeletal homeostasis remains largely elusive. Herein, through global RIPK4 ablation in adult mice, we demonstrate that RIPK4 deficiency leads to osteoporosis, promotes myeloid-biased hematopoiesis and osteolineage RIPK4 plays a crucial role in bone formation and myeloid hematopoiesis.

Thin-Layer Covalent Organic Framework-Coated Gold Superstructures for Label-Free Raman Spectroscopic Monitoring of Multiple Volatile Organic Compounds.

The detection of multiple volatile organic compounds (VOCs) encountered challenges such as sample complexity, signal reproducibility, sensitivity limitations, and interference resistance. Here, we prepared thin-layer COF-coated gold superstructures (GSP@COF) to capture and identify the fingerprint spectrum of the VOCs. The GSP@COF substrate ensured an even distribution of SERS hotspots, boosted signal intensity, and prevented nanoparticle aggregation, resulting in consistent and reliable analytical results, with less than 5% spatial variations in signal intensity.

Silver-Doped Porous Copper Catalysts for Efficient Resource Utilization of CO-Containing Flue Gases.

CO is both a key intermediate in the electrocatalytic conversion of CO and a valuable C resource, with the potential to reduce carbon emissions and mitigate the energy crisis. However, industrially emitted CO remains underutilized due to inefficiencies and economic challenges. Electrocatalytic CO reduction offers a promising approach for the efficient and environmentally friendly utilization of CO-containing flue gases.

Pre-Label-Free Three-Dimensional Imaging of Cellular Bottom Topography with a Charge-Lock Electrochemiluminescence Microscope.

Cellular bottom structure plays a vital role in cell mobility, but its narrow space makes it difficult to study. Currently, it remains challenging to panoramically map the cellular bottom topography variations due to the potential cellular damage and off-target effects of luminophores when using current ultra-high-resolution optical microscopy. Here, we present charge-lock spatiotemporal transformation electrochemiluminescence (CL-STTECL) microscopy.

Mechanistic Insights into the CO-Assisted NO Electrochemical Deoxygenation and Hydrogenation.

Electrocatalytic NO reduction to NH holds significant potential for pollutant treatment and resource recovery. Herein, we report that the introduction of CO on octahedral oxide-derived copper (o-OD-Cu) significantly enhances the electrochemical reduction of NO to NH. With 10% NO in a CO environment, the Faradaic efficiency for NH production in a flow cell remains around 80% over a wide current density range from 20 to 250 mA cm.

Boosted CO Photoreduction Performance by CdSe Nanoplatelets via Se Vacancy Engineering.

2D metal-chalcogenide nanoplatelets (NPLs) exhibit promising photocatalysis properties due to their ultrathin morphology, high surface-to-volume ratio, and enhanced in-plane electron transport mobility. However, NPLs, especially cadmium chalcogenides, encounter challenges in CO photoreduction due to insufficient solar energy utilization and fast recombination of photogenerated charge carriers. Defect engineering offers a potential solution but often encounters difficulties maintaining structural integrity, mechanical stability, and electrical conductivity.

Single molecule-driven nanomotors reveal the dynamic-disordered chemomechanical transduction of active enzymes.

Enzymes facilitate the conversion of chemical energy into mechanical work during biochemical reactions, thereby regulating the dynamic metabolic activity of living systems. However, directly observing the energy release facilitated by fluctuating individual enzymes remains a challenge, leading to a contentious debate regarding the underlying reasons for this phenomenon. Here, we aim to overcome this challenge by developing an oscillating nanomotor powered by a single-molecule enzyme, which allows real-time tracking of energy transduction in enzymatic reactions.

Constructing fecal-derived electrocatalysts for CO upcycling: simultaneously tackling waste and carbon emissions.

The escalating global fecal waste and rising CO levels present dual significant environmental challenges, further intensified by urbanization. Traditional fecal waste management methods are insufficient, particularly in addressing the related health risks and environmental threats. This study explores the synthesis of biochar from pig manure as a carbon substrate to disperse and stabilize Cu nanoparticles, resulting in the formation of an efficient Cu-NB-2000 electrocatalyst for electrocatalytic CO reduction (ECR).

Recent Advances on Integrating Porous Nanomaterials with Chemiluminescence Assays.

Advanced porous nanomaterials have recently been the subject of considerable interest due to their high surface areas, tunable pore structures, high porosity, and ease of modification. In the chemiluminescence (CL) domain, the incorporation of additional pores into nanostructures not only enhances the loading capacity for signal amplification but also allows the confinement effect in a nanoscale microreactor and the controlled release of reaction agents. In light of this, increasing efforts have been made to fabricate various porous nanomaterials and explore their potential applications in CL assays.

Direct low concentration CO electroreduction to multicarbon products via rate-determining step tuning.

Direct converting low concentration CO in industrial exhaust gases to high-value multi-carbon products via renewable-energy-powered electrochemical catalysis provides a sustainable strategy for CO utilization with minimized CO separation and purification capital and energy cost. Nonetheless, the electrocatalytic conversion of dilute CO into value-added chemicals (C products, e.g.

Anatase/TiO(B) homojunction nanosheets with a gold cocatalyst for direct photocatalytic coupling of methane to ethane.

An anatase/TiO(B) homojunction loaded with Au nanoparticles was synthesized, achieving a CH yield rate of 170 μmol g h in a flow photoreactor. The homojunction reduces TiO(B)'s strong oxidative ability, offering a more moderate environment for methane dehydrogenation, while Au aids in charge mitigation and methyl radical coupling. The catalyst highlights homojunction engineering and a ternary synergistic effect in photocatalytic CH coupling.

How local electric field regulates C-C coupling at a single nanocavity in electrocatalytic CO reduction.

C-C coupling is of utmost importance in the electrocatalytic reduction of CO, as it governs the selectivity of diverse product formation. Nevertheless, the difficulties to directly observe C-C coupling pathways at a specific nanocavity hinder the advances in catalysts and electrolyzer design for efficient high-value hydrocarbon production. Here we develop a nano-confined Raman technology to elucidate the influence of the local electric field on the evolution of C-C coupling intermediates.

Hydrothermal Valorization of Biosugars with Heterogeneous Catalysts: Advances, Catalyst Deactivation, Mitigation Strategies and Perspectives.

Sustainable production of valuable biochemicals and biofuels from lignocellulosic biomass necessitates the development of durable and high-performance catalysts. To assist the next-stage catalyst design for hydrothermal treatment of biosugars, this paper provides a critical review of (1) recent advances in biosugar hydrothermal valorization using heterogeneous catalysts, (2) the deactivation process of catalysts based on recycling tests of representative biosugar hydrothermal treatments, (3) state-of-the-art understandings of the deactivation mechanisms of heterogeneous catalysts, and (4) strategies for preparing durable catalysts and the regeneration of deactivated catalysts. Based on the review, challenges and perspectives are proposed.

Operando Spectroscopic Elucidation of the Bubble Sunshade Effect in Inorganic-Biological Hybrids for Photosynthetic Hydrogen Production.

Hydrogen production by photosynthetic hybrid systems (PBSs) offers a promising avenue for renewable energy. However, the light-harvesting efficiency of PBSs remains constrained due to unclear intracellular kinetic factors. Here, we present an operando elucidation of the sluggish light-harvesting behavior for existing PBSs and strategies to circumvent them.

Anisotropic growth of gold anchors on CdSe semiconductor quantum platelets for self-assembled architectures with well-connected electronic circuits for the electrochemical detection of enrofloxacin.

Anisotropic growth of nanomaterials enables advances in building diverse and complex architectures, which exhibit unique properties and enrich the choice of nano-building modules for electrochemical sensor devices. Herein, an anisotropic growth method was proposed to anchor gold nanoparticles (AuNPs) onto both ends of quasi-two-dimensional CdSe semiconductor quantum nanoplatelets (NPLs), appearing with a monodisperse and uniform nano-dumbbell shape. Then, these AuNPs were exploited as natural anchor points and further initiated self-assembly to create complex architectures dithiol bridges.

Fight for carbon neutrality with state-of-the-art negative carbon emission technologies.

After the Industrial Revolution, the ever-increasing atmospheric CO concentration has resulted in significant problems for human beings. Nearly all countries in the world are actively taking measures to fight for carbon neutrality. In recent years, negative carbon emission technologies have attracted much attention due to their ability to reduce or recycle excess CO in the atmosphere.

Reversibly Tuning Electrochemiluminescence with Stimulated Emission Route for Single-Cell Imaging.

Electrochemiluminescence (ECL) has established itself as an excellent transduction technique in biosensing and light-emitting device, while conventional ECL mechanism depending on spontaneous emission of luminophores lacks reversibility and tunable emission characters, limiting the universality of ECL technique in the fields of fundamental research and clinical applications. Here, we report the first observation of stimulated emission route in ECL and thus establish a reversible tuning ECL microscopy for single-cell imaging. This microscopy uses a focused red-shifted beam to transfer spontaneous ECL into stimulated ECL, which enables selective and reversible tuning of ECL emission from homogeneous solution, single particles, and single cells.

Enriching Reaction Intermediates in Multishell Structured Copper Catalysts for Boosted Propanol Electrosynthesis from Carbon Monoxide.

Fine-tuned catalysts that alter the diffusion kinetics of reaction intermediates is of great importance for achieving high-performance multicarbon (C) product generation in carbon monoxide (CO) reduction. Herein, we conduct a structural design based on CuO nanoparticles and present an effective strategy for enhancing propanol electrosynthesis from CO. The electrochemical characterization, operando Raman monitoring, and finite-element method simulations reveal that the multishell structured catalyst can realize the enrichment of C and C intermediates by nanoconfinement space, leading to the possibility of further coupling.

Accelerating ammonia synthesis in a membraneless flow electrolyzer through coupling ambient dinitrogen oxidation and water splitting.

An electrochemical approach for ammonia production is successfully developed by coupling the anodic dinitrogen oxidation reaction (NOR) and cathodic hydrogen evolution reaction (HER) within a well-designed membraneless flow electrolyzer. The obtained reactor shows the preferential yield of ammonia over nitrogen oxides on the vanadium nitride catalyst surface. At an applied oxidation potential of 2.

Two-Dimensional Fe-N-C Single-Atomic-Site Catalysts with Boosted Peroxidase-Like Activity for a Sensitive Immunoassay.

Single-atomic-site catalysts (SASCs) with peroxidase (POD)-like activities have been widely used in various sensing platforms, like the enzyme-linked immunosorbent assay (ELISA). Herein, a two-dimensional Fe-N-C-based SASC (2D Fe-SASC) is successfully synthesized with excellent POD-like activity (specific activity = 90.11 U/mg) and is used to design the ELISA for herbicide detection.