Bioremediation strategies for hexavalent chromium: Development and safety assessment of recombinant Escherichia coli strain 3458.

Incidents of periodic water pollution and damage to aquatic organisms resulting from the mismanagement of hexavalent chromium emissions have been documented. Utilizing environmentally friendly bioremediation strategies has effectively controlled chromium pollution. The ChrA gene, responsible for hexavalent chromium remediation and sourced from Sporosarcina saromensis M52, was introduced into Escherichia coli to develop recombinant strain 3458.

Spread performance and underlying mechanisms of pathogenic bacteria and antibiotic resistance genes adhered on microplastics in the sediments of different urban water bodies.

Urban water bodies often pose frequent human activities, the pollution of microplastics (MPs) in these sediments, and pathogenic bacteria and antibiotic resistance genes (ARGs) enriched on the MPs may have risk to human health. However, there is little known about these issues. In this paper, three typical urban water bodies (the urban park lake, the urban inland river, and the urban-rural lake) were selected to identify the characteristics of MPs.

Hydrogel immobilized bacteria@metal-organic-frameworks composite augmented bisphenol A removal from activated sludge and its regulation behavior on sludge community.

Improving degradation efficiency of activated sludge towards bisphenol A (BPA) is related to water safety. A hydrogel immobilized bacteria@metal-organic-frameworks (im-SQ-2@MOFs) was synthesized previously, which was a composite formed by metal organic frameworks adhering to BPA degrading bacteria. Accordingly, this study added im-SQ-2@MOFs as enhancer to augment the BPA degradation ability of activated sludge.

Roles of NOC3L and DDX17 in acquired immunodeficiency complicated with viral myocarditis and osteoporosis.

Acquired immunodeficiency syndrome is a systemic infectious disease caused by human immunodeficiency virus infection, which could attack the bones and heart. However, the relationship between Nuclear Complex Associated 3 Homolog (NOC3L) and DEAD box helicase 17 (DDX17) and acquired immunodeficiency complicated with viral myocarditis and osteoporosis is unclear. The acquired immune deficiency dataset GSE140713, GSE147162 and the osteoporosis dataset (GSE230665), and viral myocarditis dataset (GSE150392) configuration files were generated from gene expression omnibus.

New insights into uranium biomineralization mediated by Pseudomonas sp. WG2-6 in the presence of organic phosphorus: Promoting effect of extracellular polymeric substance and formation of U-P nanominerals.

Microbial biomineralization significantly affects the uranium (U) behavior in the environment. However, the mechanism of microbial biomineralization of U is still not fully understood. In this study, a dominant bacterium (Pseudomonas sp.

Label-free SELEX of aptamers for ultra-sensitive electrochemical aptasensor detection of amanitin in wild mushrooms.

Background: Mushroom poisoning poses a significant global health concern, with high morbidity and mortality rates. The primary lethal toxins responsible for this condition are alpha-amanitin (ɑ-AMA) and beta-amanitin (β-AMA). As a promising bio-recognition molecules in biosensors, aptamers, have been broadly used in the field of food detection.

The Easily Overlooked Effect of Global Warming: Diffusion of Heavy Metals.

Since industrialization, global temperatures have continued to rise. Human activities have resulted in heavy metals being freed from their original, fixed locations. Because of global warming, glaciers are melting, carbon dioxide concentrations are increasing, weather patterns are shifting, and various environmental forces are at play, resulting in the movement of heavy metals and alteration of their forms.

Real-time polymerase chain reaction detection and surgical treatment of thoracic and lumbar spondylitis due to infection: two typical case reports.

Background: Spondylitis caused by infection is a rare but challenging condition, and its successful management depends on timely diagnosis and appropriate treatment. This study reports two typical cases of thoracic and lumbar brucellosis spondylitis, highlighting the pivotal roles of real-time polymerase chain reaction (real-time PCR) detection and surgical intervention.

Case Presentation: Case 1 involved a 49-year-old male shepherd who presented with a 6-month history of fever (40°C), severe chest and back pain, and 2-week limited lower limb movement with night-time exacerbation.

The Diversity and Community Composition of Three Plants' Rhizosphere Fungi in Kaolin Mining Areas.

Mining activities in the kaolin mining area have led to the disruption of the ecological health of the mining area and nearby soils, but the effects on the fungal communities in the rhizosphere soils of the plants are not clear. Three common plants (, , and ) in kaolin mining areas were selected and analyzed their rhizosphere soil fungal communities using ITS sequencing. The alpha diversity indices (Chao1, Shannon, Simpson, observed-species, pielou-e) of the fungal communities decreased to different extents in different plants compared to the non-kauri mining area.

The characteristics of fungal responses to uranium mining activities and analysis of their tolerance to uranium.

The influence of uranium (U) mining on the fungal diversity (FD) and communities (FC) structure was investigated in this work. Our results revealed that soil FC richness and FD indicators obviously decreased due to U, such as Chao1, observed OTUs and Shannon index (P<0.05).

Comparative Study on the Efficacy of Two Perioperative Chemotherapy Regimens for Lumbar Brucellosis.

Objective: The clinical efficacy of perioperative chemotherapy regimen (rifampicin, doxycycline, levofloxacin, ceftriaxone) was evaluated for lumbar brucellosis spondylitis patients with neurological injury.

Methods: In Beijing Ditan Hospital affiliated with Capital Medical University, 32 patients with lumbar brucellosis spondylitis underwent surgery and triple perioperative chemotherapy (rifampicin, doxycycline, levofloxacin) between 2011 and 2021 due to neurological injury, and 34 patients matched up with the triple group underwent rifampicin, doxycycline, levofloxacin, and ceftriaxone. Both groups were compared in terms of changes in inflammation index, low back/leg pain, lumbar function, neurological function, and adverse drug reactions.

Analysis of the efficacy of drilling decompression autologous bone marrow and allogeneic bone grafting in the treatment of HIV-positive patients with early osteonecrosis of the femoral head.

Objective: To investigate the efficacy of treating patients with HIV-positive osteonecrosis of the femoral head using drilled decompression autologous bone marrow and allogeneic bone grafting.

Methods: 40 patients (44 hips) with early osteonecrosis of the femoral head treated by drilling decompression autologous bone marrow and allogeneic bone grafting since October 2015 were retrospectively analyzed, among which 20 patients (24 hips) were HIV-positive patients with early osteonecrosis of the femoral head, 16 males and 4 females, age 22-43 years, average 39.6 ± 10.

Effects of uranium mining on soil bacterial communities and functions in the Qinghai-Tibet plateau.

The microecological effects of plateau uranium mining are still unknown. In this study, we used 16S rRNA high-throughput sequencing to analyze the impact of plateau uranium mining on the microbial diversity and community structure of tailings soil, tunnel soil, and soil at different depths in an open pit. The results showed that uranium mining significantly reduced soil microbial community richness and diversity indicators, including Chao1, Pielou evenness, and Shannon index (P < 0.

WITHDRAWN: Analysis of the Efficacy of Drilling Decompression Autologous Bone Marrow and Allogeneic Bone Grafting in the Treatment of HIV-positive Patients with Early Osteonecrosis of the Femoral Head.

Unlabelled: The article has been withdrawn at the request of the author of the journal Current HIV Research (CHIVR). Bentham Science apologizes to the readers of the journal for any inconvenience this may have caused. The Bentham Editorial Policy on Article Withdrawal can be found at https://benthamscience.

Effects of polystyrene microplastics on the agronomic traits and rhizosphere soil microbial community of highland barley.

This study investigated the influence of polystyrene microplastics (MPs) with two different particle sizes (<1 mm, 1-5 mm) and three concentrations (1 g/m, 10 g/m, 50 g/m), as well as added degrading bacteria, on the agronomic traits of highland barley and the bacterial communities in the rhizosphere soil. Results revealed that the small particle size treatment had a significant effect on reducing the 1000-grain weight of highland barley, while the large particle size treatment had an effect on reducing the spike length, width, and awn length (P < 0.05).

A novel bacterial combination for efficient degradation of polystyrene microplastics.

This study aimed to investigate the combined decomposition of polystyrene (PS) microplastics using three bacterial cultures: Stenotrophomonas maltophilia, Bacillus velezensis, and Acinetobacter radioresistens. The ability of all three strains to grow on medium containing PS (Mn 90,000 Da, Mw 241,200 Da) microplastics as the sole carbon source was examined. After 60 days of A.

Tandem Repeat Generation and Novel Isothermal Amplification Based on Nonspecific Tailing and Replication Slippage.

Background: Isothermal amplification is considered to be one of the most promising tools for point-of-care testing molecular diagnosis. However, its clinical application is severely hindered by nonspecific amplification. Thus, it is important to investigate the exact mechanism of nonspecific amplification and develop a high-specific isothermal amplification assay.

Extracellular biomineralization of uranium and its toxicity alleviation to Bacillus thuringiensis X-27.

Uranium biomineralization can slow uranium migration in the environment and thus prevent it from further contaminating the surroundings. Investigations into the uranium species, pH, inorganic phosphate (Pi) concentration, and microbial viability during biomineralization by microorganisms are crucial for understanding the mineralization mechanism. In this study, Bacillus thuringiensis X-27 was isolated from soil contaminated with uranium and was used to investigate the formation process of uranium biominerals induced by X-27.

Better Predictive Value of Lymphocyte Count and Hemoglobin for CD4 Level of HIV Patients.

Objective: HIV patients are prone to infection and difficult to treat, which mainly manifests itself in decreased CD4 T cells in the body. Therefore, the predictive value of lymphocyte count and hemoglobin for CD4 levels in HIV patients was discussed in the prospective study.

Methods: 125 HIV patients (aged >18 or < 80 years) were recruited.

Statistical Inferences of HIVRNA and Fracture Based on the PAK1 Expression Neural Network Model.

Background: Acquired immune deficiency syndrome and fracture are all serious hazards to human health that create a widespread alarm. Biomarkers that are closely linked to HIVRNA and fracture are unknown.

Methods: 48 cases with HIV and fracture and 112 normal cases were recruited.

Efficacy of single-stage posterior surgery for HIV-positive patients with thoracolumbar tuberculosis.

Objective: We aimed to observe the clinical effect of single-stage posterior surgery on HIV-positive patients with thoracolumbar tuberculosis.

Methods: From October 2015 to October 2019, 13 HIV-positive patients with thoracolumbar tuberculosis who underwent single-stage posterior surgery were retrospectively analyzed (observation group), and 13 HIV-negative patients with thoracolumbar tuberculosis who were matched with the gender, age, operative site, and surgical approach during the same period were selected as the control group. Postoperative complications, hemoglobin, albumin, CD4T lymphocyte count, operative site, operative time, and blood loss were recorded between the two groups.

Beneficial influence of single-stage posterior surgery for the treatment of lumbar brucella spondylitis combined with spondylolisthesis.

We aimed to evaluate the clinical efficacy of the single-stage posterior surgical treatment for patients of lumbar brucella spondylitis combined with spondylolisthesis. In this study, we performed a retrospective analysis of 16 patients with lumbar brucellosis spondylitis combined with spondylolisthesis from January 2015 to January 2019. All patients underwent single-stage posterior lumbar debridement, reduction, interbody fusion, and instrumentation.