Feature-driven whole-tissue imaging with subcellular resolution.

Existing microscopy approaches are often unable to identify and contextualize rare but biologically meaningful events due to limitations associated with simultaneously achieving both high-resolution imaging and a cm-scale field of view. Here, we present multiscale cleared tissue axially swept light-sheet microscopy (MCT-ASLM), a platform combining cm-scale imaging with targeted high-resolution interrogation of intact tissues in human-guided or autonomous modes. Capable of capturing fields of view up to 21 mm at micron-scale resolution, MCT-ASLM can seamlessly transition to a targeted imaging mode with an isotropic resolution that approaches ∼300 nm.

The Impact of Perioperative Comfort Nursing Combined With Targeted Operating Room Nursing on Postoperative Stress Response and Nursing Satisfaction in Colorectal Cancer Patients.

Aim: This study aims to explore the impact of perioperative comfort nursing combined with targeted operating room nursing on the stress response and nursing satisfaction in colorectal cancer patients, emphasizing the importance of precision nursing in enhancing postoperative recovery.

Methods: This retrospective study analyzed clinical data of colorectal cancer patients (n = 165) who underwent laparoscopic colorectal surgery at our hospital between February 2022 and February 2024. Grouped according to different perioperative nursing methods, and the patients were divided into two groups: the experimental group (n = 85) and the control group (n = 80).

Development and validation of a predictive nomogram for postoperative upper limb lymphedema in breast cancer patients: a retrospective cohort study.

Upper limb lymphedema is a common and debilitating complication following breast cancer surgery. Identifying patients at high risk for developing lymphedema is crucial for early intervention and improved outcomes. This study aimed to develop and validate a predictive nomogram for estimating the risk of postoperative upper limb lymphedema in breast cancer patients.

Spread performance and underlying mechanisms of pathogenic bacteria and antibiotic resistance genes adhered on microplastics in the sediments of different urban water bodies.

Urban water bodies often pose frequent human activities, the pollution of microplastics (MPs) in these sediments, and pathogenic bacteria and antibiotic resistance genes (ARGs) enriched on the MPs may have risk to human health. However, there is little known about these issues. In this paper, three typical urban water bodies (the urban park lake, the urban inland river, and the urban-rural lake) were selected to identify the characteristics of MPs.

Study on the Erosion Law and Erosion Prediction Model of Pipe Columns During Gas Storage Reservoir Injection and Production Processes.

There are few studies on erosion problems in gas storage environments. High-speed gas-carrying sand in gas storage wells can cause pipeline erosion and subsequent failure. To this end, a numerical model of gas/liquid/solid three-phase erosion under high temperature and high-pressure conditions in gas storage was established.

Population pharmacokinetics and dose optimization of voriconazole in patients with COVID-19-associated pulmonary aspergillosis.

Objectives: The study aimed to investigate the pharmacokinetic profile of voriconazole in patients with COVID-19-associated pulmonary aspergillosis (CAPA) to optimize dosing strategies.

Methods: Population pharmacokinetic modeling was conducted using clinical data from CAPA patients to analyze voriconazole's pharmacokinetic behavior. A one-compartment model with first-order elimination was employed to characterize voriconazole disposition.

Hydrogel immobilized bacteria@metal-organic-frameworks composite augmented bisphenol A removal from activated sludge and its regulation behavior on sludge community.

Improving degradation efficiency of activated sludge towards bisphenol A (BPA) is related to water safety. A hydrogel immobilized bacteria@metal-organic-frameworks (im-SQ-2@MOFs) was synthesized previously, which was a composite formed by metal organic frameworks adhering to BPA degrading bacteria. Accordingly, this study added im-SQ-2@MOFs as enhancer to augment the BPA degradation ability of activated sludge.

Corrigendum: Population pharmacokinetics and dosing optimization of imipenem in Chinese elderly patients.

[This corrects the article DOI: 10.3389/fphar.2024.

Remodeling of bacterial nitrilase active pocket improves the capability to degrade glucosinolate-derived nitriles.

Nitriles exhibit acute cytotoxicity to human and animal cells. Nitrilase is a green biocatalyst that can directly convert nitrile into nontoxic carboxylic acids and ammonia. However, the nitrilases capable of degrading 3-butenenitrile and 4-pentenenitrile derived from glucosinolate present in rapeseed meals are still limited.

Predictive factors for 30-day mortality after polymyxin B treatment of carbapenem-resistant Gram-negative bacilli infections.

Objectives: This study aimed to investigate the associated factors predicting 30-day mortality of carbapenem-resistant Gram-negative bacilli (CR-GNB) infections, with focus on polymyxin B AUC/MIC.

Methods: This prospective cohort study included patients with CR-GNB infections from January 2022 to January 2024. The primary outcome was 30-day mortality.

Feature-Driven Whole-Tissue Imaging with Subcellular Resolution.

Understanding how subcellular structures and events shape tissue-wide phenomena remains a central challenge in biology. We present Multiscale Cleared Tissue Axially Swept Light-Sheet Microscopy (MCT-ASLM), a platform combining cm-scale imaging with targeted high-resolution interrogation of intact tissues in human-guided or autonomous modes. Capable of capturing fields of view up to 21 mm at micron-scale resolution, MCT-ASLM can seamlessly transition to submicron (~300 nm) isotropic resolution for targeted imaging.

Population pharmacokinetics and dosing optimization of imipenem in Chinese elderly patients.

Objectives: To assess the pharmacokinetics and pharmacodynamics of imipenem in a retrospective cohort of hospitalized Chinese older patients.

Methods: A population pharmacokinetic (PPK) model was constructed utilizing a nonlinear mixed-effects modeling approach. The final model underwent evaluation through bootstrap resampling and visual predictive checks.

Targeting caspase-8: a new strategy for combating hepatocellular carcinoma.

Hepatocellular carcinoma (HCC) represents the most prevalent form of primary liver cancer and has a high mortality rate. Caspase-8 plays a pivotal role in an array of cellular signaling pathways and is essential for the governance of programmed cell death mechanisms, inflammatory responses, and the dynamics of the tumor microenvironment. Dysregulation of caspase-8 is intricately linked to the complex biological underpinnings of HCC.

Nitrilase GiNIT from Efficiently Degrades Nitriles Derived from Rapeseed Meal Glucosinolate.

Rapeseed meal is severely restricted in its utilization as unconventional animal feed due to anti-nutritive compounds, such as glucosinolate, that are degraded to toxic nitriles such as 3-butenenitrile and 4-pentenenitrile in animals. Few studies on nitrilases that can degrade glucosinolate-derived nitriles have been reported thus far. In the present study, a nitrilase gene from was over-expressed in and the purified recombinant nitrilase rGiNIT showed specific activities of 134.

Water quality assessment and its pollution source analysis from spatial and temporal perspectives in small watershed of Sichuan Province, China.

Rapid socio-economic development has led to many water environmental issues in small watersheds such as non-compliance with water quality standards, complex pollution sources, and difficulties in water environment management. To achieve a quantitative evaluation of water quality, identify pollution sources, and implement refined management in small watersheds, this study collected monthly seven water quality indexes of four monitoring points from 2010 to 2023, and ten water quality indexes of 23 sampling points in the Shiting River and Mianyuan River which are tributaries of the Tuojiang River Basin. Then, water quality evaluation and pollution source analysis were conducted from both temporal and spatial perspectives using the Water Quality Index (WQI) method, the Absolute Principal Component Scores/Multiple Linear Regression (APCS-MLR) method, and the Positive Matrix Factorization (PMF) receptor modeling technique.

Mutual regulation of novel transcription factors RsrD and RsrE positively modulates the production of raw-starch-degrading enzyme in .

Filamentous fungi can produce raw-starch-degrading enzyme, however, regulation of production of raw-starch-degrading enzyme remains poorly understood thus far. Here, two novel transcription factors raw-starch-degrading enzyme regulator D (RsrD) and raw-starch-degrading enzyme regulator E (RsrE) were identified to participate in the production of raw-starch-degrading enzyme in . Individual knockout of and in the parental strain Δ resulted in 31.

A RsrC-RsrA-RsrB transcriptional circuit positively regulates polysaccharide-degrading enzyme biosynthesis and development in Penicillium oxalicum.

Filamentous fungi produce polysaccharide-degrading enzymes, which is controlled by poorly understood transcriptional circuits. Here we show that a circuit comprising RsrC-RsrA-RsrB (Rsr: production of raw-starch-degrading enzyme regulator) that positively regulates production of raw starch-degrading enzymes in Penicillium oxalicum. Transcription factor (TF) RsrA is essential for biosynthesis of raw starch-degrading enzymes.

A positive FOXP3/lncRNA SNHG1 feedback axis ameliorates cardiomyocytes hypertrophy by negatively regulating Parkin-mediated mitophagy.

In this study, we identified FOXP3 as a transcription factor for lncRNA SNHG1, which exerts a significant protective role against cardiomyocyte hypertrophy. Through DNA-pull down experiments and ChIP analysis, we confirmed that FOXP3 could bind to the promoter of SNHG1. Luciferase reporter and RT-qPCR experiments validated that FOXP3 overexpression promoted SNHG1 expression in cardiomyocytes.