Microfluidic organoid-slice-on-a-chip system for studying anti-cholangiocarcinoma drug efficacy and hepatorenal toxicity.

Organ-chip technology, in contrast to cell culture and animal models, offers a promising platform for accelerating drug development. However, current chip designs simulate human organ functions and there is a lack of multi-organ chip designs that can simultaneously study drug efficacy and hepatorenal toxicity. Here, we developed a novel microfluidic multi-organ chip that integrated cholangiocarcinoma organoids (CCOs) with recellularized liver slices (RLS) and recellularized kidney slices (RKS), to simultaneously assess anti-cholangiocarcinoma drug efficacy and hepatorenal toxicity.

UCP2 attenuates neural apoptosis and inflammation in spinal cord injury by inducing the acetylation of ANXA1 and activating the PI3K/AKT pathway.

Spinal cord injury (SCI) represents a prevalent form of mechanical trauma, frequently resulting in significant disability and mortality. Uncoupling protein 2 (UCP2) has been recognized for its neuroprotective properties; however, its specific role in SCI remains to be elucidated. This study aims to investigate the neuroprotective effects of UCP2 in the context of SCI and to further explore its downstream mechanisms of action.

Long-term exposure to air pollution and gastrointestinal disease: findings from a nationwide cohort study in China.

Background And Aims: Air pollution poses significant risks to human health, but its impact on gastrointestinal (GI) health remains underexplored. This study assesses the long-term effects of air pollution on GI diseases using data from the China Health and Retirement Longitudinal Study (CHARLS).

Methods: This nationwide cohort study utilized CHARLS data from participants recruited in 2011, followed by surveys in 2013, 2015, 2018, and 2020.

Mechanism of KDM4A in Regulating Microglial Polarization in Ischemic Stroke.

Microglia polarization plays important roles in inflammatory processes after ischemic stroke. This study aimed to explore the mechanism of lysine-specific histone demethylase 4 (KDM4A) in microglia polarization after ischemic stroke. The mouse model was established using middle cerebral artery occlusion/reperfusion (MCAO/R) and the cell model was established by oxygen-glucose deprivation/reperfusion (OGD/R).

Clinical metabolomics in type 2 diabetes mellitus: from pathogenesis to biomarkers.

As a multidimensional metabolic disorder, the disability and death rate of type 2 diabetes mellitus (T2DM) has increased over time. T2DM covers a wide range of pathological manifestations ranging from hyperglycemia to multi-organ failure, and it has the potential to evolve into acute complications, including ketosis and chronic complications such as peripheral neuropathy, retinopathy, and nephropathy. T2DM mainly occurs in microvascular and large vessels and thus it is restricted for the clinician to diagnose and prescribe.

Single-cell transcriptomics analysis reveals that the tumor-infiltrating B cells determine the indolent fate of papillary thyroid carcinoma.

Objective: Active surveillance (AS) offers a viable alternative to surgical intervention for the management of indolent papillary thyroid carcinoma (PTC), helping to minimize the incidence of unnecessary treatment. However, the broader adoption of AS is hindered by the need for more reliable diagnostic markers. This study aimed to identify the differences between indolent and progressive PTC and find new targets for biomarker development and therapeutic strategies.

Evaluation of Safety and Pharmacokinetics of DDCI-01, a Phosphodiesterase Type 5 Inhibitor, in Healthy Participants.

Aim: The aim of this study was to evaluate the safety, tolerability, and pharmacokinetics of single escalating oral doses of DDCI-01 (a novel, highly selective, long-acting phosphodiesterase type 5 inhibitor) administered via capsules to healthy volunteers.

Methods: This randomized, double-blind, placebo-controlled, single ascending dosing, Phase Ia clinical study involved 52 healthy volunteers who were randomized (3:1 ratio) to receive a single oral dose of DDCI-01 (1.25, 2.

A Report of Primary Pyogenic Ventriculitis Caused by Diagnosed by Metagenomic Next-Generation Sequencing.

Background: Primary ventriculitis is a rare but serious brain infection characterized by inflammation of the ependyma and purulence within the ventricular system. Due to the challenges in early diagnosis and the potential for suboptimal treatment, this condition carries a significant risk of complications such as recurrence, hydrocephalus, and death. Metagenomic next-generation sequencing (mNGS) enables the rapid and broad-spectrum identification of pathogens, facilitating timely and precise diagnosis.

Exploring the glucose-lowering and anti-inflammatory immune mechanism of artemether by AMPK/mTOR pathway and microbiome based on multi-omics.

Background: Diabetes mellitus (DM) is a metabolic disease with high morbidity, which significantly affects human life and health expenditures. Previous studies have demonstrated that artemether (ATM) has anti-diabetes and anti-inflammation activities, but its mechanism has not been fully elucidated. This research aimed to elucidate the impact of ATM on glucolipid metabolism in a type 2 diabetes mellitus (T2DM) model db/db mice and what kind of role the gut microbiota played, and explored the underlying mechanisms involved.

Prognostic value of serum lipids in newly diagnosed acute promyelocytic leukemia.

Background And Purpose: Dyslipidemia has been linked to acute promyelocytic leukemia (APL), with abnormal lipid metabolism observed during treatment. However, its role in APL pathogenesis remains unclear. This study investigates the relationship between serum lipid levels and clinical features, risk stratification, bleeding tendency, and prognosis of newly diagnosed APL patients, focusing on the role of the PTK2 gene in regulating lipid metabolism and its potential as a therapeutic target.

A nomogram for predicting cancer-specific survival in patients with locally advanced unresectable esophageal cancer: development and validation study.

Background: Immunotherapy research for esophageal cancer is progressing rapidly, particularly for locally advanced unresectable cases. Despite these advances, the prognosis remains poor, and traditional staging systems like AJCC inadequately predict outcomes. This study aims to develop and validate a nomogram to predict cancer-specific survival (CSS) in these patients.

Profile of gene defects in human genetic diseases: 46,XY disorders of sex development.

The RNA helicase gene is involved in ribosomal biological processes, and linked to human genetic diseases associated with 46,XY disorders of sex development (46,XY DSD) or neurodevelopment. Recently, relevant reports have primarily focused on 46,XY DSD. However, there is still a lack of overall understanding of the genetic characteristics, phenotype, etc.

Evolutionary Insight and Expression Pattern of WUSCHEL-Related Homebox Genes of .

The () gene family is critical for plant growth, development, and the regulation of stress responses; however, the function of WOX has not been extensively studied. Nine genes have been identified in the genome. These genes were unequally distributed on five chromosomes, with four genes occupying chr1.

Efficient fabrication of hunting trap-inspired degradable film with enhancing antimicrobial activity and reducing fomite transmission.

The extensive application of medical protective polymers has significantly contributed to preserving human lives and well-being. Due to the widespread use of protective polymer materials and their non-degradable nature, environmental and biological pollution issues are becoming increasingly severe. To address the environmental pollution problems caused by traditional polymer materials, this study proposes an efficient and successive method for the mass production of polylactic acid/graphene oxide (MNPG) films with the hunting trap-inspired pyramidal micro/nanostructure by combining micro-extrusion compression molding and electrostatic flocking.

TRIM22 inhibits the metastasis of colorectal cancer through facilitating β-Catenin degradation.

Tripartite motif-containing 22 (TRIM22), a member of the tripartite motif protein family, has emerged as a putative tumor suppressor in various cancers. Nevertheless, its specific role and clinical significance in colorectal cancer (CRC) remain poorly characterized. Herein, we observed that TRIM22 expression was frequently downregulated in primary CRC tissues and was significantly correlated with better prognosis.

Immunomodulatory and growth-promoting effects of root powder in broiler chicks challenged with Gallinarum.

Background: Amid growing concern about antimicrobial resistance due to the irrational use of antibiotics in treating common poultry diseases, particularly which is a foodborne pathogen in humans. This study investigates the effects of ethnoveterinary supplementation of (L. Benth.

Association of escitalopram-induced shifts in gut microbiota and sphingolipid metabolism with depression-like behavior in wistar-kyoto rats.

The microbiota-gut-brain axis plays a pivotal role in neuropsychiatric disorders, particularly in depression. Escitalopram (ESC) is a first-line antidepressant, however, its regulatory mechanisms on the microbiota-gut-brain axis in the treatment of depression remain unclear. The antidepressant effects of ESC were evaluated using the forced swim test in Wistar-Kyoto (WKY) rats, while damage in the gut and brain regions was assessed through H&E staining and immunohistochemistry.

LAMP3 exacerbates autophagy-mediated neuronal damage through NF-kB in microglia.

Background And Purpose: Cerebral ischemia/reperfusion (IR) after ischemic stroke causes deleterious microglial activation. Lysosomal associated membrane protein 3 (LAMP-3) has been indicated play a role in autophagy, yet the specific role of LAMP3 in microglia autophagy during cerebral ischemia and reperfusion (I/R) injury (CIRI) is unknown.

Methods: The oxygen-glucose deprivation/reperfusion (OGD/R) model and middle cerebral artery occlusion/reperfusion (MCAO/R) model were established.

Analysis of molecular resistance and associated risk factors in tuberculosis.

Background: Local surveillance of molecular resistance to first-line anti-tuberculosis (TB) drugs and fluoroquinolones (FQs) was initiated in 2019, but existing reports remain limited, hindering effective TB prevention and control efforts. This study aims to investigate molecular resistance to first-line anti-TB drugs and FQs, assess risk factors for FQs resistance, and provide insights into the spread of drug-resistant tuberculosis (DR-TB) to inform more effective control and treatment strategies.

Methods: Sputum samples from 25,150 non-duplicate patients attending 10 designated TB medical institutions across Luoyang City and all county and township areas under its jurisdiction from January 2019 to December 2023 were analyzed via fluorescence real-time PCR to detect Mycobacterium tuberculosis complex (MTBC)-positive strains.

Elevated serum levels of HIF-1α and VEGF as potential biomarkers in connective tissue disease-associated pulmonary arterial hypertension.

We to measure the serum levels of hypoxia-inducible factor-1α (HIF-1α) and vascular endothelial growth factor (VEGF) in patients diagnosed with connective tissue disease (CTD)-associated pulmonary arterial hypertension (PAH) (CTD-PAH) and to analyze their clinical implications. Thirty patients who were diagnosed with CTD-PAH via right heart catheterization (RHC) were enrolled in the observation group. Additionally, twenty CTD patients without PAH (CTD-non-PAH) were enrolled in the CTD-non-PAH group, and twenty healthy participants were enrolled in the healthy control group.

Clinical characteristics and genetic analysis of a patient with diffuse pulmonary lymphangiomatosis: a case report and literature review.

Background: Diffuse pulmonary lymphangiomatosis (DPL) is a rare pulmonary disorder, which affects the lymphatic channels from the mediastinum to the pleura. DPL is often misdiagnosed or missed due to the lack of clear specificity and definitive medical therapies. In most cases, the disease progresses to chronic morbidity or even death.

gastrointestinal digestion simulation screening of novel ACEI peptides from broccoli: mechanism in high glucose-induced VSMCs dysfunction.

Many natural angiotensin-converting enzyme inhibitory (ACEI) peptides have been widely studied. However, their stability is poor in most cases. In this study, peptides were initially digested from broccoli , and absorption was simulated by Caco2 cells transport and then analyzed by Peptideomics and molecular docking.

The role of activated partial thrombin time in mediating the impact of poorly glycemic control on diabetic peripheral neuropathy in patients with type 2 diabetes mellitus.

Aim: This study aims to investigate the role of activated partial thrombin time (APTT) as a potential mediator in the relationship between suboptimal glycemic control and diabetic peripheral neuropathy (DPN) in individuals with type 2 diabetes mellitus (T2DM).

Methods: A total of 183 T2DM patients were enrolled in this study. Comprehensive clinical data, including coagulation parameters and nerve conduction velocity, were collected and compared between patients with and without DPN.

Allele-specific methylation of SSTR4 associated with aging and cognitive functions in patients with schizophrenia.

The co-occurrence of alcohol use disorder (AUD) and schizophrenia is prevalent, with a rate of 33.7%. Previous research has suggested a genetic and epigenetic overlap between these two disorders.