Aquaporin 2 is differentially expressed in granulosa cells of various stages of human follicles and is regulated by luteinizing hormone.

Introduction: Several aquaporins (AQPs) are involved in the influx of water to form follicular fluid, and AQP2 may play a crucial role in follicular growth. However, the specific roles of Aquaporin (AQP) 2 and AQP6 in granulosa cells (GCs) during follicular fluid (FF) formation, as well as their relationship with gonadotropins (Gn), remain unclear.

Methods: Luteinized granulosa cells (LGCs) were isolated from follicles of different diameters.

Worked human bones and the rise of urban society in the neolithic Liangzhu culture, East Asia.

The Liangzhu culture, which emerged in the mid-Holocene Yangtze Delta, established one of East Asia's earliest cities. At its core site, Liangzhu, numerous worked human bones were unearthed from canal and moat deposits. These remains date from 5000 to 4550 cal BP, with a concentration between 4800 and 4600 cal BP.

Endothelial progenitor cell-derived microvesicles therapy relieves myocardial infarction symptoms by altering left ventricular protein expression.

Objective: To investigate the therapeutic potential of endothelial progenitor cell-derived microvesicles (EPC-MVs) in a rat myocardial infarction (MI) model, focusing on their effects on inflammation, apoptosis, and global proteomic changes in the left ventricle.

Methods: Endothelial progenitor cells (EPCs) were isolated from mouse bone marrow, and microvesicles (MVs) were derived and injected into rats with MI induced by ligation of the left anterior descending artery. Therapeutic efficacy was assessed by measuring inflammatory cytokines (TNF-α, IL-6) and cardiac injury markers (creatine kinase-MB, myoglobin), along with histologic and apoptotic analyses.

Intraoperative navigation system for liver resection based on edge-AI and multimodal AI.

Background: Traditional intraoperative navigation methods show insufficient adaptability in dynamic surgical environments. The rapid development of Artificial Intelligence (AI) presents an opportunity to overcome these limitations, making the construction of real-time, adaptive intraoperative navigation systems a key research goal. This study, based on Edge-AI and multimodal AI technologies, aims to develop and evaluate a foundational system for achieving real-time, offline intraoperative navigation and warnings during minimally invasive liver surgery.

Lead-induced hypertension and cognitive dysfunction: brain amyloid pathology.

Background: Lead (Pb) exposure is a recognized environmental risk factor for cognitive decline and may aggravate Alzheimer's disease (AD) pathology through hypertension-related mechanisms. However, the specific role of mineralocorticoid receptor (MR) signaling in this process remains unclear.

Objectives: This study investigated whether Pb-induced hypertension exacerbates amyloid pathology via MR activation, and evaluated the therapeutic effects of amlodipine and spironolactone in an AD mouse model.

Synthesis, Fungicidal Activity, and Action Mode Investigations of Novel Nitrophenyl ()-3,3-Diaryl Acrylates.

Uncoupling agents involved in the oxidative phosphorylation process are a category of bioactive molecules that have garnered significant attention; however, their applications in agriculture remain limited. In this study, we employed a scaffold-hopping strategy to design and synthesize a new class of phenyl acrylates. This was achieved by substituting the methylacryloyl group in dinocap with 3,3-diarylacryloyl derivatives featuring a pyridine ring, which shows affinity for mitochondrial respiratory chain complexes.

Low Testosterone Level and Mortality Risk in Patients With Prostate Cancer: A Post-Randomization Analysis.

Background: A low serum testosterone can prolong the time needed for PSA to exceed normal and prompt a work-up to rule out prostate cancer (PC), delaying diagnosis. We evaluated PC aggressiveness at diagnosis and PC-specific and all-cause mortality (PCSM, ACM)-risk within comorbidity subgroups in patients with low versus normal testosterone.

Methods: Between 2005 and 2015, 350 PSA-screened patients with tumor (T) category1c-4N0M0 unfavorable-risk PC were enrolled in a randomized trial and comprised the study cohort.

Weighted sum and order statistics methods for dynamic information borrowing in basket trials.

In basket trials, the same investigational therapy is studied on multiple sub-populations simultaneously under a single protocol. The goal of basket trials is to identify the sub-populations in which the therapy is effective. Basket trials have become a popular and generally accepted study design in disease areas including but not limited to oncology and rare diseases, for their advantages in operation and ethical considerations.

Hsa-miRNA-16-5p Of Urinary Exosomes A Reliable Biosignature Effective For Prostate Cancer Screening.

Introduction: MicroRNAs (miRNAs) are small, non-coding RNA molecules critical for cellular function, growth, and development. Recent advances in remote diagnostic technologies have highlighted the potential of urinary miRNAs as non-invasive biomarkers for disease monitoring. This study introduces a simple, rapid, and cost-effective reagent for exosomal miRNA extraction, designed for urine-based exosome screening.

Pain, fatigue, and associated gene expressions over chemotherapy in patients with colorectal cancer.

Context: Patients with colorectal cancer undergoing chemotherapy often experience significant pain and fatigue. Limitations in understanding the complex phenotypes and biological mechanisms of these symptoms hinder effective interventions.

Objectives: This study aimed to identify the pain and fatigue patterns during one chemotherapy cycle and associated gene expression profiles.

Unprecedented Flux-Mediated Synthesis of Crystalline Polyimide Covalent Organic Frameworks Using the 4-Dimethylaminopyridine (DMAP)-Type Nucleophilic Catalyst for Use in Efficient Photosynthesis of HO.

Photocatalytic HO production from water and O represents a sustainable approach for clean chemical synthesis, but developing efficient and stable photocatalysts remains challenging. Herein, we report a novel 4-dimethylaminopyridine (DMAP)-type nucleophilic catalyst-mediated flux synthesis strategy to prepare crystalline polyimide covalent organic frameworks (PI-COFs) as efficient photocatalysts for HO generation. Interestingly, by introducing molten DMAP as the reaction medium, we achieved three PI-COFs (DMAP-BNCOFs, DMAP-BBCOFs, and DMAP-NNCOFs) with significantly enhanced crystallinity and porosity compared to those obtained in conventional solvothermal synthesis.

A -unique effector with the CxNC motif enhances plant NDPK2 kinase activity to suppress plant immunity.

fungi cause destructive diseases among a wide range of hosts worldwide. We found that effector CfEC92 from specifically binds ATP through an unidentified ATP-binding domain, leading to changes in the protein secondary structure. The residues Cys, Asn, and Cys were critical for ATP binding with CfEC92, and mutations at these sites impaired the ability to suppress host immunity.

An Integrated Tandem-Structured Separator Enables Dual-Enhanced Stable Interfaces for Long-Cycle-Life and High-Areal-Capacity Aqueous Zinc-Iodine Batteries.

Aqueous zinc-iodine batteries (AZIBs) hold great promise for next-generation energy storage technologies due to their inherent safety, cost-effectiveness, and environmental friendliness. However, unstable interfacial chemistry manifested as polyiodide formation and accumulation at the cathode, alongside dendrite growth and hydrogen evolution reaction (HER) at the anode results in capacity loss. Moreover, polyiodide migration toward the anode further triggers severe Zn corrosion, exacerbating interfacial instability.

Systematic identification and analysis of WRKY transcription factors reveals the role of MrWRKY14 in .

Bayberry () is a significant subtropical fruit tree, renowned for its distinctive flavor and high nutritional value. WRKY transcription factors are a class of plant-specific zinc-finger proteins that play critical roles in plant growth and development, secondary metabolism, and responses to abiotic stress. However, there is currently limited information about the gene family in bayberry.

High-Output Droplet Electricity Generator for Intelligent Self-Powered Biochemical Analysis.

The detection and analysis of chemical and biological substances are crucial in fields such as pharmaceuticals, disease diagnosis food safety and environmental monitoring. However, traditional analytical methods often involve complex procedures, expensive equipment, and time-consuming processes, making it challenging to achieve high sensitivity, reliability, portability, and real-time detection. To overcome these challenges, we propose a miniaturized intelligent self-powered e-tongue (MISET) that introduces key innovations in liquid sensing technology.

Penicilazaphilone C triggers ferroptosis in triple-negative breast cancer cells via the MDM2/p53/SLC7 A11/GPX4 pathway.

Penicilazaphilone C (PAC) is a novel azaphilone isolated by our research team. While known for its antitumor properties, the potential of PAC to trigger ferroptosis in triple-negative breast cancer (TNBC) cells remains unexplored. This study aims to assess the ferroptotic induction capability of PAC and elucidate the underlying molecular mechanisms.

A review on the ethnopharmacology, metabolites, pharmacological uses, and toxicology of Vahl.

Vahl, a member of the Moraceae family and genus , is a traditional medicinal plant with diverse metabolites. Traditionally, Vahl has been used in Asian countries to treat various ailments including indigestion, loss of appetite, cold, pneumonia, tuberculosis, cough, asthma, excessive sweating, backache, bruises, arthritis, liver diseases, skin conditions, gynecological disorders, and pediatric illnesses. As a botanical drug with both medicinal and nutritional applications, Vahl is an integral part of China's multi-ethnic medical system, serving as a tonic for many years.

Toxicarioside H induces ferroptosis in triple-negative breast cancer cells through Nrf2/HO-1 pathway.

Recent studies have identified novel cardiac glycosides from natural sources with potential anti-tumor properties. Toxicarioside H (ToxH) is a novel cardiac glycoside isolated by our collaborative research team. However, its ability to induce ferroptosis in triple-negative breast cancer (TNBC) cells has not been investigated.

Development of a DNA Aptamer-Based Approach to Noninvasively Image CAR-T Cells In Vivo and Traceless Enrichment In Vitro.

Chimeric antigen receptor (CAR) T cells offered a potential cure for malignancies, however, their outcomes and dynamics across different anatomical sites remained inadequately characterized. Monitoring the bio-distribution and tumor-homing of CAR-T cells in vivo is crucial, as it provides patient-specific data that might inform on treatment success, potential failure, and off-target toxicities. Herein, an Aptamer A3 by Cell-SELEX (systematic evolution of ligands by exponential enrichment) is generated, which can bind with CAR-T cells with nanomolar affinity.

Impaired Mitochondrial Biogenesis Inhibits Epithelial-Mesenchymal Transition in Villi of PCOS Patients.

Context: Polycystic ovary syndrome (PCOS) is accompanied by impaired mitochondrial biogenesis in the ovary and uterus. Whether impaired mitochondrial biogenesis exhibits in villi of PCOS, and its effect and underlying mechanism remain unclear.

Objective: This work aimed to investigate mitochondrial biogenesis status and effect on villi of PCOS patients.

Expression and clinical significance of IGF-1 and PDGF in aqueous humor and serum of diabetic patients with visually significant cataract.

This study aimed to analyze the expression and clinical significance of IGF-1 and PDGF in the aqueous humor and serum of diabetic patients with visually significant cataract. A retrospective study was conducted on 136 diabetic patients with visually significant cataract who underwent phacoemulsification. Patients were divided into non-complication (n = 82) and complication groups (n = 54) based on postoperative outcomes.

Reconfigurable acoustic tweezer for precise tracking and in-situ sensing of trace miRNAs in tumor cells.

MicroRNAs (miRNAs) have emerged as critical biomarkers for early cancer diagnosis and monitoring. However, their isolation from clinical samples typically yields only trace amounts, significantly limiting the sensitivity and efficiency of cancer detection. To address this challenge, we present a octangular reconfigurable acoustic tweezer (ORAT) as an integrated platform for precise tumor cell tracking and in-situ detection of trace miRNAs.