Complexed Micelles System Based on Ceria Oxide Nanozyme for Bone Remodeling and ROS Scavenging in Periodontitis.

Periodontitis is a chronic inflammatory disease caused by dental plaque and characterized by excessive accumulation of reactive oxygen species (ROS). Current treatments often fail to eliminate persistent inflammation and regenerate damaged tissue. Here, an ROS-responsive multifunctional micelle system (HA-PMs@Ce) constructed by self-assembly of the block copolymer polyethylene glycol-polylysine/phenylboronic acid (PLLA-b-PLys/PBA) and tannic acid (TA) is proposed, encapsulating cerium oxide (CeOx) nanozyme and coated with hyaluronic acid (HA).

Sulfamoylbenzoic Acid Derivatives as Molecular Additives through Fine Dipole Moment Regulation for Highly Reproducible Perovskite Solar Cells.

An excellent reproducibility of high-quality perovskite thin films is imperative for the commercialization of perovskite solar cells. Multifunctional additive engineering is a promising strategy to tackle this challenge as it facilitates simultaneous control over crystallization processes and defect passivation. Here, an innovative strategy is proposed, leveraging -OH, -NH, and -Cl to precisely tune the dipole moment and electronic configuration of multifunctional modified 4-chloro-3-sulfonamide benzoic acid (CSBA) through substituent inductive and conjugation effects.

Controlling transient and coupled diffusion with pseudoconformal mapping.

Diffusion in physical, chemical, and biological systems often occurs under transient conditions and involves coupling across multiple physical fields, challenging conventional control methods limited to steady-state, single-field settings. Here, we present a general geometric framework for regulating diffusion in time-dependent and multiphysics-coupled environments based on pseudoconformal mapping. This method preserves material isotropy and ensures smooth interface matching, enabling robust and flexible modulation of diffusion governed by Fick's second law and beyond.

Angiogenic apoptotic vesicle-laden silk fibroin /sodium alginate hydrogel for pulp regeneration.

Angiogenesis remains a key challenge in pulp regeneration due to the ischemic and hypoxic root canal environment. Apoptotic vesicles (ApoVs) offer unique advantages as a breakthrough strategy due to their stability in hypoxic environments, resistance to immune clearance, and high yield. However, a critical gap persists in biomaterials capable of spatiotemporal ApoVs delivery for pulp regeneration.

Thread design optimization of a dental implant using explicit dynamics finite element analysis.

To optimize the thread design of a commercial dental implant for ideal stress distribution in the peri-implant bone. The models of the BLT Φ4.1 × 10 implant (Institut Straumann AG) and the alveolar bone were created.

Advances in photonic crystal hydrogels for biomedical research: A review.

Photonic crystal hydrogel is an advanced material that combines the properties of photonic crystal and hydrogel with unique responsiveness and optical properties. By combining photonic crystals with hydrogels, the new material not only inherits the optical properties of photonic crystals, but also possesses the responsiveness and good biocompatibility of hydrogels. This makes the photonic crystal hydrogel show a broad application prospect in the field of sensors, clinical detection and biomedicine.

Engineered Macrophage Membrane-Camouflaged Nanodecoys Reshape the Infectious Microenvironment for Efficient Periodontitis Treatment.

A vicious cycle between microbiota dysbiosis and hyperactivated inflammation, hardly disrupted by conventional therapies, remains a significant clinical challenge for periodontitis treatment. Herein, by cloaking a cascade catalysis system in an engineered macrophage membrane, a nanodecoy-based strategy, with targeted bacteria-killing and immunomodulatory abilities, is proposed for reshaping the hostile periodontitis microenvironment. Specifically, recombinant human antimicrobial peptide, LL-37, is anchored to a Toll-like receptor-enriched macrophage membrane via genetic engineering, which facilitates the specific bacteria elimination and efficient tissue retention of the nanodecoys.

A high-fructose diet leads to osteoporosis by suppressing the expression of Thrb and facilitating the accumulation of cholesterol.

Osteoporosis is classified as a metabolic syndrome, and the consumption of fructose has been linked to various metabolic diseases. However, the specific effects and underlying mechanisms of fructose on bone health remain inadequately understood. In this study, we demonstrate that fructose intake can exacerbate bone loss in murine models by facilitating the accumulation of cholesterol within the bones.

Single-cell sequencing reveals PHLDA1-positive smooth muscle cells promote local invasion in head and neck squamous cell carcinoma.

Background: Smooth muscle cells within the tumor microenvironment play a crucial role in cancer progression. However, their involvement in the local invasion of head and neck squamous cell carcinoma remains poorly understood. In this research, we aim to investigate the role of smooth muscle cells-mediated cell interactions in facilitating the local invasion of head and neck squamous cell carcinoma.

Enhancement Effect of Static Magnetic Field on Bactericidal Activity.

The biological effects of magnetic fields are pervasive in microorganisms, with significant attention given to alternating magnetic fields (AMFs). However, AMFs induce electrical and magnetothermal effects, which complicate the interpretation of magnetic field-induced biological effects and introduce uncertainties regarding cytotoxicity in practical applications. The static magnetic field (SMF) with few variables and high biocompatibility presents a promising alternative for both understanding biological mechanisms and ensuring safe applications, but has a remaining problem on weak interactions with microorganisms.

Selenium-modified hydroxyapatite titanium coating: enhancing osteogenesis and inhibiting cancer in bone invasion by head and neck squamous cell carcinoma.

Introduction: Head and neck squamous cell carcinoma (HNSCC) frequently invades the jaw, and surgical treatment often leads to bone defects requiring reconstruction with titanium plates. To enhance the anti-tumor and bone regeneration properties of titanium, a selenium-modified hydroxyapatite coating was developed on titanium surfaces.

Methods: Selenium-modified hydroxyapatite coatings was fabricated using micro-arc oxidation (MAO).

An axolotl limb regeneration-inspired strategy to enhance alveolar bone regeneration.

Guided bone regeneration (GBR) is widely applied in implant dentistry, employing barrier membranes to create an osteogenic space by preventing gingival tissue ingrowth. However, this method does not enhance the osteogenic capacity of osteoblasts, limiting sufficient bone volume in larger defects. Inspired by axolotl limb regeneration, abundant soft tissue-derived stem cells mobilized to the defect may facilitate comprehensive osteogenesis within a BMP-2-enriched environment.

Evaluation of the Pink Esthetic Score for Single Implants in the Esthetic Zone Using Intraoral Scanning Data: A Proof-of-Concept Study.

Objectives: To evaluate the feasibility of esthetic assessments based on intraoral scanning data using pink and white esthetic scores (PES/WES).

Materials And Methods: Thirty samples with both intraoral photographs and scanning data were collected and rated by two observers with excellent consistency independently. PES includes seven variables, and WES includes five.

Removal of resin cement using a picosecond laser: An in vitro study.

Statement Of Problem: The rapid and safe removal of resin cement from tooth surfaces is challenging. Whether removal with a picosecond laser might providing a practical solution is unclear.

Purpose: The purpose of this in vitro study was to explore the effect and mechanism of a picosecond laser acting on resin cement and whether the dental tissue is damaged.

Ephrin-B2 acts as a positive regulator of osteogenesis-angiogenesis coupling.

Aims: The aim of this study was to explore the role and potential mechanisms of Ephrin-B2 signaling in osteogenesis-angiogenesis coupling and to provide guidance for periodontal tissue engineering.

Materials And Methods: Considering the close relationship between periodontitis and periodontal bone defects, single-cell transcriptomic data from periodontal tissues in published databases (GEO number: GSE171213) were analyzed to characterize Ephrin-B2 signaling between osteoblastic lineages and endothelial cells. To observe the effects and mechanisms of Ephrin-B2 in angiogenesis and periodontal bone regeneration, osteoblasts were constructed that overexpress Ephrin-B2 and were then co-cultured with human umbilical vein cells (HUVECs).

Prediction of pink esthetic score using deep learning: A proof of concept.

Objectives: This study aimed to develop a deep learning (DL) model for the predictive esthetic evaluation of single-implant treatments in the esthetic zone.

Methods: A total of 226 samples, each comprising three intraoral photographs and 12 clinical features, were collected for proof of concept. Labels were determined by a prosthodontic specialist using the pink esthetic score (PES).

Photodynamic and photothermal bacteria targeting nanosystems for synergistically combating bacteria and biofilms.

The escalating hazards posed by bacterial infections underscore the imperative for pioneering advancements in next-generation antibacterial modalities and treatments. Present therapeutic methodologies are frequently impeded by the constraints of insufficient biofilm infiltration and the absence of precision in pathogen-specific targeting. In this current study, we have used chlorin e6 (Ce6), zeolitic imidazolate framework-8 (ZIF-8), polydopamine (PDA), and UBI peptide to formulate an innovative nanosystem meticulously engineered to confront bacterial infections and effectually dismantle biofilm architectures through the concerted mechanism of photodynamic therapy (PDT)/photothermal therapy (PTT) therapies, including in-depth research, especially for oral bacteria and oral biofilm.

PRMT1-methylated MSX1 phase separates to control palate development.

Little is known about the regulation and function of phase separation in craniofacial developmental disorders. MSX1 mutations are associated with human cleft palate, the most common craniofacial birth defect. Here, we show that MSX1 phase separation is a vertebrate-conserved mechanism underlying embryonic palatal fusion.

17β-estradiol promotes osteogenic differentiation of BMSCs by regulating mitophagy through ARC.

The study aims to elucidate the mechanism through which 17β-estradiol facilitates osteogenic differentiation in bone marrow mesenchymal stem cells (BMSCs). In our study, lentiviral transfection was employed to establish apoptosis repressor with caspase recruitment domain (ARC) knockdown or overexpression in BMSCs. The impact of 17β-estradiol on ARC expression was assessed using western blot, RT-PCR and immunofluorescence.

The glycerol stabilized calcium phosphate cluster for rapid remineralization of tooth enamel by a water-triggered transformation.

Remineralization is a common strategy for the repair of early demineralized tooth enamels, but the harsh dynamic oral environment often hampers its efficacy. Rapid remineralization is expected to address this challenge, however, the stabilizers of remineralization materials often resist their transformation required for repair. Here, by dissolving the ions of calcium and phosphate in glycerol-dominant solvents, we obtain the calcium phosphate clusters (1-2 nm), which are stabilized by glycerol (with high viscosity and affinity to clusters), but can perform a fast enamel repair via the water-triggered transformation in both static and dynamic environments.

Femtomolar hydrogen sulfide detection via hybrid small-molecule nano-arrays.

Early disease diagnosis hinges on the sensitive detection of signaling molecules. Among these, hydrogen sulfide (HS) has emerged as a critical player in cardiovascular and nervous system signaling. On-chip immunoassays, particularly nanoarray-based interfacial detection, offer promising avenues for ultra-sensitive analysis due to their confined reaction volumes and precise signal localization.

Engineering Dual Active Sites and Defect Structure in Nanozymes to Reprogram Jawbone Microenvironment for Osteoradionecrosis Therapy.

Four to eight percent of patients with head and neck cancer will develop osteoradionecrosis of the jaw (ORNJ) after radiotherapy. Various radiation-induced tissue injuries are associated with reactive oxygen and nitrogen species (RONS) overproduction. Herein, Fe doping is used in VO (Fe-VO) nanozymes with multienzyme activities for ORNJ treatment via RONS scavenging.

Self-Reinforcing Ionogel Bioadhesive Interface for Robust Integration and Monitoring of Bioelectronic Devices with Hard Tissues.

Integrating bioelectronic devices with hard tissues, such as bones and teeth, is essential for advancing diagnostic and therapeutic technologies. However, stable and durable adhesion in dynamic, moist environments remains challenging. Traditional bioadhesives often fail to maintain strong bonds, especially when interfacing with metal electrodes and hard tissues.

Conditioned Extracellular Vesicles Derived from Dedifferentiated Fat Cells Promote Bone Regeneration by Altering MicroRNAs.

: Extracellular vesicles (EVs) derived from stem cells demonstrate significant potential in bone regeneration. Adipose tissue is regarded as a stem cell reservoir with abundant reserves and easy accessibility. Compared to adipose-derived stem cells (ASCs), dedifferentiated fat cells (DFATs) possess similar stem cell characteristics but exhibit greater proliferative capacity, higher homogeneity, and an enhanced osteogenic differentiation potential.

Sensitive and specific detection of trace Al ions using an upconversion nanoparticle-xylenol orange complex the inner filter effect.

We have developed a novel fluorescence sensor based on upconversion nanoparticles (UCNPs) for the rapid and sensitive detection of trace aluminum ions (Al). The sensor utilizes the inner filter effect (IFE) between the UCNPs and the xylenol orange-aluminum complex (XO-Al), resulting in significant fluorescence quenching at 543 nm upon Al binding. This quenching correlates directly with the Al concentration, allowing for quantitative detection within a range of 0-30 μM and achieving an ultra-low detection limit of 0.