In silico search reveals the association of lichens with black yeast-like fungi in the order Chaetothyriales.

Lichens exemplify a unique symbiotic relationship between fungi and algae or cyanobacteria, where fungi (mycobionts) provide structural support, while algae or cyanobacteria (photobionts) provide nutrients. Recent discoveries in the order Chaetothyriales have led to the description of several lichenicolous species, underscoring an intricate relationship of some black yeast-like fungi with lichens. The present study aims to investigate public metagenomic data of lichens available in the SRA database, covering a total of 2888 samples.

Sand and dust storms exacerbate the toxicity of particle pollution on mortality: A cohort study among 1.5 million Chinese older adults.

Objective: The impact of desert-originated dust has been underestimated in fine particulate matters (PM)-related disease burden studies. This study aimed to assess the association of long-term dust PM exposure and all-cause mortality among older adults in China.

Methods: A cohort study using electronic health records (2010-2020) across Weinan, a city in northwest China, which experiences persistently high PM levels and frequent sand and dust storms, included 1,553,724 adults aged ≥45 years.

Peroxisome Proliferator-Activated Receptor Gamma (PPARγ)-targeted Gel/Mg/Lip@Gigantol nanoplatform attenuates skin barrier disruption-associated aging in mice via NLRP3 suppression.

Skin aging serves as a critical indicator of systemic health decline. Despite Peroxisome Proliferator-Activated Receptor Gamma (PPARγ) being a key therapeutic target, mechanistic understanding remains incomplete and potent, safe activators are lacking, hindering clinical progress. This study proposes the "Barrier-Skin-Systemic Aging Axis," demonstrating that epidermal barrier disruption accelerates aging via PPARγ suppression.

A combination of tetramethylpyrazine and berberine prevents coronary microembolization in rats by regulating miR-34a-5p in platelet-derived exosomes.

Ethnopharmacological Relevance: Both chuanxiong rhizome and Coptis chinensis were first recorded in the Shennong's Classic of Materia Medica. Chuanxiong rhizome and Coptis chinensis are a classic herbal pair in Traditional Chinese Medicine (TCM), renowned for their effects in activating blood circulation and resolving toxicity. They are widely used to treat chest impediment and heart pain.

JPH203 alleviates renal fibrosis via inhibition of serine-related mTORC1 pathway in TGF-β1-induced fibroblasts and UUO mice.

Renal fibrosis is the common pathological outcome of chronic kidney disease (CKD) progressing into end-stage renal disease. The excessive proliferation of fibroblasts plays an important role in the CKD progression. Nutrients such as amino acids and their transportation are essential for cell proliferation.

MMSupcon: An image fusion-based multi-modal supervised contrastive method for brain tumor diagnosis.

The diagnosis of brain tumors is pivotal for effective treatment, with MRI serving as a commonly used non-invasive diagnostic modality in clinical practices. Fundamentally, brain tumor diagnosis is a type of pattern recognition task that requires the integration of information from multi-modal MRI images. However, existing fusion strategies are hindered by the scarcity of multi-modal imaging samples.

Small extracellular vesicles orchestrated pathological communications between breast cancer cells and cardiomyocytes as a novel mechanism exacerbating anthracycline cardiotoxicity by fueling ferroptosis.

Small extracellular vesicles (sEVs) critically orchestrate inter-tissue and inter-organ communications and may play essential roles in heart-tumor interaction. However, whether cancer-secreted sEVs affect the progression of doxorubicin-induced cardiotoxicity (DOXIC) via orchestrating the tumor cell-cardiomyocyte crosstalk has not yet been explored. Herein, we reveal that Doxorubicin (DOX)-treated breast cancer cells secrete sEVs (D-BCC-sEVs) that exacerbate DOX-induced ferroptosis of human iPSC-derived cardiomyocytes (hiCMs).

U-shaped association between post-stroke cognitive impairment and high-density lipoprotein cholesterol at the acute period of stroke.

Post-stroke cognitive impairment (PSCI) imposes a significant economic and social burden on patients and their families. High-density lipoprotein cholesterol (HDL-C) is reported to have protective effects on cognitive function in older adults. This study assesses the effects of HDL-C during the acute period of stroke on PSCI.

A retrospective study on Mohs micrographic surgery combined with adjuvant radiotherapy in the treatment of extramammary Paget's disease: analysis of 87 patients.

Purpose: This retrospective study assessed the effectiveness of Mohs micrographic surgery (MMS) combined with adjuvant radiotherapy for the treatment of extramammary Paget's disease (EMPD).

Methods: This retrospective study included 87 patients with pathologically confirmed EMPD and complete follow-up data who were treated at the Radiation Therapy Department of Peking University First Hospital between January 2012 and December 2021. The surgical approach for the primary lesion involved MMS, followed by postoperative radiotherapy with doses ranging from 50 to 60 Gy administered over 25-30 fractions.

The interaction between the skin microbiome and antimicrobial peptides within the epidermal immune microenvironment: Bridging insights into atopic dermatitis.

The epidermal immune microenvironment is a multifaceted system in which the interplay between the skin microbiome and antimicrobial peptides plays a pivotal role in sustaining skin homeostasis and preventing dysbiosis. Disruption of these interactions can lead to inflammatory skin conditions such as atopic dermatitis. This review aims to explore the complex mechanisms by which antimicrobial peptides and the skin microbiome communicate within the epidermal immune microenvironment, emphasizing causal dynamics and the dual role of antimicrobial peptides.

Neuro-Immuno-Stromal Context in Colorectal Cancer: An Enteric Glial Cell-Driven Prognostic Model via Machine Learning Predicts Survival, Recurrence, and Therapy Response.

Background: Enteric glial cells (EGCs) have been implicated in colorectal cancer (CRC) progression. This study aimed to develop and validate a prognostic model integrating EGC- and CRC-associated gene expression to predict patient survival, recurrence, metastasis, and therapy response.

Methods: Bulk and single-cell RNA sequencing data were analyzed, and a machine learning-based model was constructed using the RSF random forest algorithm.

Deep learning-assisted system improves practical effect in cervical cytopathology diagnosis: A comparative study of reading modes.

Deep learning (DL) has significantly improved the diagnostic accuracy and efficiency of cytopathologists. However, current DL-assisted reading modes have yet to be fully evaluated, and there is limited evidence regarding cytopathologists' preferences and experiences. This study employs a randomized, controlled, four-way crossover design to assess the effectiveness of four different reading modes in cervical cytopathology readings.

Ethanol Infusion of the Vein of Marshall in Catheter Ablation of Persistent Atrial Fibrillation in Patients with Mitral Valve Replacement.

Background: The effectiveness of ethanol infusion of the vein of Marshall (EIVOM) for persistent atrial fibrillation (AF) in patients with mitral valve replacement (MVR) remains to be determined.

Objectives: This study investigated the effectiveness and safety of EIVOM in catheter ablation of persistent AF in patients with MVR.

Methods: This is a retrospective case-control study.

Optimal stimulation site and fiber tracts in subthalamic deep brain stimulation for Meige syndrome.

Background: Deep brain stimulation (DBS) of the subthalamic nucleus (STN) has emerged as an effective therapy for Meige syndrome (MS). However, the optimal stimulation site within STN and the most effective stimulation fiber tracts have not been investigated.

Methods: Based on the discovery cohort (n = 65), we first identified the optimal stimulation site within the STN using the sweet spot mapping method.

An HIV fusion-inhibitory lipopeptide provides robust post-exposure prophylaxis and prevents viral reservoir seeding in macaques.

LP-98 is a lipopeptide HIV fusion inhibitor showing strong treatment and pre-exposure prophylaxis efficacies in non-human primates. In this study, we further characterized its pharmacokinetics, long-lasting antiviral activity, and post-exposure prophylaxis (PEP) efficacy using 62 macaques. In cynomolgus macaques, LP-98 achieved high concentrations (C) with a half-life (T) of ∼31 h, and sustained an effective therapeutic concentration for two weeks post-injection.

Zwitterionic Sulfadiazine-Based Micelle Achieves Effective Drug Delivery to Glioblastoma by Overcoming Multiple Biological Barriers.

Multiple biological barriers severely restrict the delivery efficiency of nanoparticles (NPs) to tumors. To overcome biological barriers, traditional NPs usually require a complex design, which increases the difficulty of clinical translation. Therefore, there appears to be a dilemma between the complex biological barriers and clinical requirement for a simple molecular structure of NPs.

FPR2 Agonism Attenuates Restenosis by Mitigating Neointimal Hyperplasia via ELOVL6.

Restenosis following endovascular intervention in lower extremity arterial disease contributes to significant morbidity and mortality. This study investigates the role of formylpeptide receptor 2 (FPR2) in neointimal hyperplasia and evaluates the therapeutic potential of the selective FPR2 agonist BMS-986235 in mitigating restenosis. FPR2 expression was significantly reduced in the popliteal and anterior tibial arteries of male amputees with restenosis compared to healthy controls.

The association between the uric acid to high-density lipoprotein cholesterol ratio (UHR) index and obesity in postmenopausal women: a cross-sectional analysis based on the NHANES.

Background: Obesity has emerged as a critical global public health challenge. Postmenopausal women experience significantly elevated risks of metabolic disorders and a marked increase in obesity prevalence due to declining estrogen levels. The uric acid to high-density lipoprotein cholesterol ratio (UHR), an emerging biomarker for metabolic syndrome, is gaining clinical recognition.

A multi-tissue single-cell expression atlas in cattle.

Systematic characterization of the molecular states of cells in livestock tissues is essential for understanding the cellular and genetic mechanisms underlying economically and ecologically important physiological traits. Here, as part of the Farm Animal Genotype-Tissue Expression (FarmGTEx) project, we describe a comprehensive reference map including 1,793,854 cells from 59 bovine tissues in calves and adult cattle, spanning both sexes, which reveals intra-tissue and inter-tissue cellular heterogeneity in gene expression, transcription factor regulation and intercellular communication. Integrative analysis with genetic variants that underpin bovine monogenic and complex traits uncovers cell types of relevance, such as spermatocytes, responsible for sperm motility and excitatory neurons for milk fat yield.

Development and validation of a nomogram for predicting 30-day outcomes in patients with unruptured vertebrobasilar dissecting aneurysms undergoing endovascular treatment: a multicenter cohort study.

Background: Endovascular treatment (EVT) of vertebrobasilar dissecting aneurysms (VBDAs) is associated with high morbidity and mortality, significantly influencing patient prognosis. This study aimed to develop and validate a nomogram for predicting 30-day outcomes in patients with unruptured VBDAs undergoing EVT.

Methods: This retrospective study included 606 patients with unruptured VBDAs who underwent EVT at 10 institutions between January 2015 and April 2025, with 491 in the training cohort and 115 in the validation cohort.

Midterm outcomes of rheumatic mitral valve repair versus replacement in a dual-centre retrospective study.

Background: Rheumatic mitral valve disease remains a major global health challenge. Determining optimal surgical approaches is critical. This study aimed to identify key repairability factors and compare midterm outcomes of mitral repair versus replacement.

Aldehyde dehydrogenase 2 deficiency impairs liver progenitor cell proliferation in alcohol-fed mice.

Aldehyde dehydrogenase 2 (ALDH2) is a critical enzyme involved in the detoxification of acetaldehyde. Although numerous studies have demonstrated the significance of ALDH2 in alcohol-associated liver disease (ALD), its role in alcohol-induced activation of liver progenitor cells (LPCs) has not been thoroughly investigated. Proteomic analysis of serum samples from patients with either normal ALDH2 genotype or ALDH2 mutation following alcohol consumption revealed that ALDH2 deficiency may suppress LPC proliferation.

Combination of photodynamic therapy and estrogen cream for menopause women with cervical low-grade squamous intraepithelial lesion with human papillomavirus infection.

Background: In menopausal women, decreased estrogen levels lead to genital tract mucosal atrophy and reduced mucosal immune function. Low-grade squamous intraepithelial lesions (LSIL) associated with persistent or newly acquired high-risk HPV infection may progress silently to high-grade squamous intraepithelial lesions (HSIL) and eventually cervical cancer.

Objective: This study aimed to evaluate the efficacy and safety of photodynamic therapy combined with estrogen in the treatment of menopausal women with LSIL and high-risk HPV infection.