Mendel randomization confirmed gastroesophageal reflux disease may increase the risk of mental disorders.

Background: The potential causal relationship between gastroesophageal reflux disease (GERD) and mental disorder was analyzed using the mendelian randomization (MR) method.

Methods: Data are derived from genome-wide association study (GWAS) summary data, using gastroesophageal reflux disease (GERD) as the exposure factor. Single nucleotide polymorphisms (SNPs) significantly associated with GERD were selected as instrumental variables (IVs), and mental disorders (bipolar disorder, major depression, Alzheimer's disease, anorexia nervosa, anxiety, and obsessive-compulsive disorder) were used as outcome variables.

Liquiritigenin Restores Declined Oocyte Quality and Improves Embryo Development in Aged Mice.

Ovarian aging leads to a decline in oocyte quality and reduced reproductive potential, which is one of the main challenges faced by assisted reproductive technology (ART). Oxidative stress (OS) is a major contributor to this decline. In this study, we investigated the protective effects of natural flavonoid compound liquiritigenin (LQ) on oocyte maturation and embryo development in aged mice.

Different titration protocols in pain management after radical gastrectomy for gastric cancer patients.

Background: Effective pain management after radical gastrectomy is crucial for patient recovery. With the promotion of enhanced recovery after surgery protocols, postoperative pain management has become a core component of perioperative care. However, controversy remains regarding the optimal frequency of analgesic titration for pain control.

production of CAR T cell: Opportunities and challenges.

Chimeric antigen receptor T (CAR T) cell therapy has achieved remarkable efficacy for patients with hematological malignancies. However, viral vector-mediated production of CAR T cells is time-consuming and expensive and impairs T cell function. On one hand, an elaborate manufacturing process not only impairs the function of CAR T cells but also limits its usage in patients with rapidly progressing diseases.

Alkaline phosphatase-instructed self-assembling supramolecular glucosamine hydrogel for osteoarthritis treatment.

Osteoarthritis (OA) is the most prevalent musculoskeletal disorder, affecting hundreds of millions of people worldwide. Glucosamine (GlcN) has been shown to effectively reduce proteoglycan degradation, attenuate articular cartilage degeneration and joint space narrowing, and alleviate osteoarthritis-related pain. However, the use of GlcN as a long-term medication for alleviating osteoarthritis is limited by its short half-life.

Determinants of thoracic aortic calcification and its effects on thoracic aortic size.

Thoracic aortic calcification (TAC) is a valuable predictor for assessing the risk of cardiovascular disease. However, there is limited evidence clarifying the specific determinants of TAC and its impact on the thoracic aortic diameter. A total of 446 patients who underwent lung nodule screening between August 2020 and April 2023 were included.

Development and validation of a visual nomogram for predicting clinically significant prostate cancer in negative mpMRI using Ga-PSMA PET/CT.

Multi-parametric magnetic resonance imaging (mpMRI) is a valuable medical technology for detecting clinically significant prostate cancer (csPCa). The diagnostic accuracy of mpMRI for csPCa in negative mpMRI (PI-RADS 1-2) remains suboptimal, underscoring the need for improvements for csPCa. This study aimed to build a visual predictive nomogram for early detection of csPCa in negative mpMRI.

Negative pressure mechanical signal increases the phosphorylation of eNOS Ser1177 by upregulating HSP90 expression to promote wound angiogenesis.

Background: Chronic wound pathogenesis involves impaired angiogenesis. While negative pressure wound therapy (NPWT) clinically promotes angiogenesis, its biomechanical mechanisms remain unclear.

Methods: A mechanical stretching model simulating NPWT was established in vitro.

Cross-attention graph neural networks for inferring gene regulatory networks with skewed degree distribution.

Background: Inferring Gene Regulatory Networks (GRNs) from gene expression data is a pivotal challenge in systems biology. Most existing methods fail to consider the skewed degree distribution of genes, complicating the application of directed graph embedding methods.

Results: The Cross-Attention Complex Dual Graph Embedding Model (XATGRN) was proposed to address this issue.

S100A8/A9 perturbation in bone marrow blunts antitumor immunity by promoting protumorigenic myelopoiesis in mouse models.

S100A8/A9 plays a critical role in the formation of an immunosuppressive tumor microenvironment. Therefore, it is important to identify inhibitors targeting S100A8/A9 to enhance antitumor immunity. However, systemic targeting of S100A8/A9 in clinical trials has shown minimal effects.

Mechanism of crosstalk between Helicobacter pylori and immune stromal cells in the development and occurrence of gastric cancer.

Helicobacter pylori (Hp) has been classified as a class I carcinogen in gastric cancer, and one of the important mechanisms by which it affects the gastric environment and promotes cancerogenesis is by triggering inflammation.Inflammatory responses caused by Hp's 'crosstalk' with various types of cells in the microenvironment of the gastric tumour play a key role in cancer progression, but the exact mechanism of its 'crosstalk' is still unclear. This paper focuses on the regulatory impact of Hp infection on the tumor microenvironment, systematically explores the activation process of immune stromal cells such as tumor-associated macrophages (TAMs), cancer-associated fibroblasts (CAFs), mesenchymal stem cells (MSCs), and myeloid-derived suppressor cells by directly or indirectly promoting the secretion of inflammatory factors by Hp, and analyzes the molecular mechanism of these cells that influence the occurrence and development of gastric cancer through multiple signal pathways, in order to provide innovative theoretical basis for the development of accurate prevention and treatment strategies for Hp infection-related gastric cancer.

Siramesine induced cell death of glioblastoma through inactivating the STAT3-MGMT signaling pathway.

Background: Glioblastoma (GBM), the most prevalent invasive primary brain tumor in adults, is characterized by high mortality, frequent recurrence, and short survival time. The effectiveness of temozolomide (TMZ) in treating GBM is compromised by chemoresistance. Elevated levels of O-methylguanine DNA methyltransferase (MGMT) and the activation of signal transduction and activator of transcription 3 (STAT3) have been associated with GBM’s resistance to TMZ chemotherapy.

Donor Planarity-Induced Molecular Twist in Donor-Acceptor-Donor AIEgens for Efficient NIR-II Fluorescence and Photothermal Therapy.

Aggregation-induced emission (AIE) active donor-acceptor-donor (D-A-D) small molecules represent promising candidates for second near-infrared fluorescence imaging (NIR-II FLI) guided photothermal therapy (PTT) in anti-tumor applications. Trianiline and tetraphenylvinyl analogues are often used as electron donors, but may have limitations, such as moderate electron donor capabilities or suboptimal AIE properties. Herein, a molecular design strategy integrating π-conjugated planar electron donors are reported within a structurally twisted D-A-D framework to develop improved AIE-type NIR-II probes.

Signatures of miRNA 5-methylcytosine modification profile and potential immune-related target gene regulation in diabetic kidney disease.

Background: Diabetic kidney disease (DKD) is a major microvascular complication of diabetes, with microRNAs (miRNAs) playing a role in its pathogenesis through methylation modifications. Preliminary experiments indicated an association between 5-methylcytosine (m5C) modification and miRNA dysregulation in peripheral blood mononuclear cells (PBMCs) of DKD patients.

Methods: We employed Arraystar's small RNA modification chip to detect m5C modifications in miRNAs from PBMCs of DKD patients and predicted target genes.

Blood metabolites as mediators in erectile dysfunction: insights from a multi-center proteomics and genetic study.

Objective: This study aims to identify circulating proteins causally associated with erectile dysfunction (ED) using Mendelian randomization (MR) analysis.

Methods: We utilized two of the largest multi-center proteomics databases as exposures and the FinnGen database as the outcome source. A large-scale two-sample MR analysis, including coloc colocalization analysis and SMR (Summary data-based Mendelian Randomization) analysis, was conducted to evaluate the reliability of proteomic effects on ED outcomes.

Automatic target-seeking nanoparticle inhibiting orthotopic drug-resistant colon cancer and liver metastases via regulating cancer cell adhesion and proliferation.

Galectin-3 (Gal-3) plays an important role in adhesion and proliferation of cancer cells. The level of Gal-3 in blood and the expression of Gal-3 in colon cancer tissue are significantly increased in patient with colon cancer. The elevated Gal-3 promotes the migration and drug resistance of colon cancer.

Overcoming temozolomide resistance in glioma: recent advances and mechanistic insights.

Temozolomide (TMZ) remains the cornerstone chemotherapy for glioma, yet intrinsic and acquired resistance mechanisms significantly limit its clinical effectiveness. This review summarizes the multifaceted molecular pathways contributing to TMZ resistance, including enhanced DNA repair mechanisms such as O-methylguanine-DNA methyltransferase (MGMT), mismatch repair (MMR), and base excision repair (BER). Additional resistance factors include genetic mutations that affect the drug response, dysregulated non-coding RNAs (miRNAs, lncRNAs, and circRNAs), glioma stem cells (GSCs), cytoprotective autophagy, an immunosuppressive tumor microenvironment (TME), altered signaling pathways, and active drug efflux transporters.

Integrative single-cell and bulk transcriptomics with Mendelian randomization reveals NAD-related biomarkers in diabetic retinopathy: Insights and experimental validation.

Background: Metabolic dysregulation involving nicotinamide adenine dinucleotide (NAD) has been implicated in the pathogenesis of various diseases; however, its role in diabetic retinopathy (DR) remains poorly understood. This study aimed to identify NAD-associated biomarkers and elucidate their molecular mechanisms in DR.

Methods: DR-related data were retrieved from public databases.

A CoQ10 analog ameliorates cognitive impairment and early brain injury after subarachnoid hemorrhage by regulating ferroptosis and neuroinflammation.

Subarachnoid hemorrhage (SAH) represents a stroke subtype that can lead to prolonged cognitive deficits as well as death or disability. Prior investigation has suggested that CoQ10 analogs can mitigate oxidative stress and inflammation and promote mitochondrial biogenesis in the context of brain injury and neurodegenerative disorders. However, the precise mechanisms underlying early brain injury (EBI) following SAH remain incompletely understood, and the detailed molecular processes have yet to be completely clarified.

Impact of Laboratory-Derived Frailty Index on Clinical Outcomes in Critical Care Patients with COPD: A Retrospective Analysis Using the MIMIC-IV Database.

Background And Objective: Chronic obstructive pulmonary disease (COPD) is associated with high mortality and morbidity worldwide. Notably, 20% of COPD patients are admitted to the ICU, and among them, there is a 25% mortality rate. Therefore, identifying novel risk factors for effective intervention is crucial for managing COPD.

A nomogram risk prediction model for ischemic mitral regurgitation after primary percutaneous coronary intervention in patients with ST-segment elevation myocardial infarction.

Aim: This study developed a nomogram to predict the risk of ischemic mitral regurgitation (IMR) after percutaneous coronary intervention (PCI) in patients with ST-segment elevation myocardial infarction (STEMI) patients and evaluate their long-term prognosis.

Methods: Data from 342 STEMI patients were collected. Logistic regression identified independent risk factors for IMR during hospitalization, while Cox regression assessed risk factors during follow-up.

Bone metastases diminish extraosseous response to checkpoint blockade immunotherapy through osteopontin-producing osteoclasts.

Bone metastatic lesions typically associate with suboptimal responses to immune checkpoint blockade (ICB) therapies. In this study, we observed that across multiple clinical cohorts and a variety of mouse models, the presence of osseous metastases induces ICB resistance in extraosseous tumors. Mechanistically, this long-distance communication is mediated by osseous tumor-conditioned osteoclasts producing osteopontin (OPN).

Pathophysiological changes in incentive processing in episodic migraine: a preliminary event-related potential study.

This study examined pathophysiological changes in incentive processing in migraineurs. Nineteen episodic migraine (EM) patients and 19 healthy controls (HCs) performed a monetary incentive delay task while their event-related potentials were recorded. During the incentive anticipation phase, both Cue-N2 and Cue-P3 amplitudes were responsive to incentive cues in both groups, indicating no between-group differences in the distinct anticipatory subprocesses that underly incentive cue evaluation.

Polyprenylated acylphloroglucinols from and their antidepressant activities.

Three new polycyclic polyprenylated acylphloroglucinols, attenuatumines A-C (), were ioslated from the 90% EtOH extract of the air dried aerial parts of . Structural elucidation of all the compounds was performed by spectral methods such as 1D and 2D (H-H COSY, HMQC, and HMBC) NMR spectroscopy. All the isolated compounds were evaluated for their antidepressant activity by inhibiting reuptake of tritiated serotonin ([H]-5-HT) and noradrenalinet ([H]-NE) in rat brain synaptosomes.