GhSPX1s Interact with GhPHR1A and GhPHL1A in Regulating Phosphate Starvation Response in Cotton.

SPX () family genes play a pivotal role in phosphorus signaling, phosphorus uptake, and phosphorus translocation in plants. However, to date, the SPX family genes have not been systematically investigated in cotton. In this study, we conducted a genome-wide analysis and identified 44 SPX family genes in , classifying them into four subfamilies (SPX, SPX-MFS, SPX-EXS, and SPX-RING) based on conserved domains.

Effect of radical radiotherapy dose on the prognosis of limited-stage thoracic small cell carcinoma of the esophagus: A multicenter retrospective trial from China (CHiSCEC).

Background: The optimal radiation dose for the treatment of small cell carcinoma of the esophagus (SCEC) has not been established. This study aimed to investigate the treatment outcomes and toxicities in patients with limited-stage SCEC (LS-SCEC) treated by radiotherapy.

Methods: Patients with LS-SCEC from 14 institutions were retrospectively identified between December 2000 and October 2021.

A π-Conjugated Molecular Bridge Strategy for Constructing Efficient Hole Transport Pathways in Inverted Perovskite Solar Cells.

Metal halide perovskite solar cells (PSCs) hold promise for next-generation photovoltaics but are restricted by suboptimal efficiency and poor long-term stability. In inverted PSC architectures, self-assembled monolayers (SAMs) are widely employed as hole-selective layers (HSLs) due to their favorable energy-level alignment and negligible parasitic absorption. However, traditional SAMs often exhibit weak intermolecular interactions, leading to film aggregation, poor interfacial contact, and severe nonradiative recombination.

Recent Advances of Aggregation-Induced Emission Luminogens in Atherosclerotic Cardiovascular Disease.

Atherosclerotic cardiovascular disease (ASCVD), the primary cause of mortality worldwide, is distinguished by the development of atherosclerotic plaques due to lipid accumulation in the artery wall, ultimately resulting in a range of severe cardiovascular and cerebrovascular complications. In recent years, aggregation-induced emission luminogens (AIEgens), which possess the advantages of fluorescence enhancement in aggregated states, exceptional photostability, and finely tunable biocompatibility, have emerged as a promising candidate for the diagnosis and treatment of ASCVD. This review systematically summarizes the research advancements of AIEgens in ASCVD over the past five years, mainly including the properties of AIEgens and the design for ASCVD management, the application of AIEgens in ASCVD biomarkers sensing, imaging and treatment of ASCVD.

Assessment of docosahexaenoic acid's preventive efficacy against unconjugated bilirubin neurotoxicity through MRP1 protein expression.

Objective: Jaundice in neonates promotes unconjugated hyperbilirubinemia (UCB), which causes neurological disorders, and its management remains a challenge. This study evaluated the protective effect of docosahexaenoic acid (DHA) against UCB-induced neurotoxicity.

Method: Astrocyte cell lines were prepared and separated into four different groups: SAL group; DHA group treated with 50 µM DHA; UCB group treated with 50 µM UCB; and UCB + DHA group treated with 50 µM UCB along with 50 µM DHA.

Cisplatin-induced WWP1-eccDNA expression contributes to ovarian cancer resistance.

Background: Multidrug resistance remains a major obstacle in the treatment of ovarian cancer (OC) patients. Recent research has underscored the critical role of extrachromosomal circular DNA (eccDNA) in tumor initiation and progression. However, there is limited comprehensive understanding of the role eccDNA plays in tumor resistance.

Nitrogen addition substantially affects plant phenology in terrestrial ecosystems: a meta-analysis.

Introduction: Phenology is a sensitive biological indicator of climate change. Increasing nitrogen (N) deposition has amplified phenological shifts, making their study across terrestrial ecosystems crucial for understanding global change responses. While existing research focuses on single ecosystems, comparative analyses are lacking.

Concentration-function coupled electrolytes harmonize thermodynamics and kinetics for stable zinc metal batteries.

The application of zinc-based aqueous batteries (ZABs) is limited by poor thermodynamic stability and sluggish electrochemical kinetics due to the unfavorable bulk phase and interface. Conventional electrolyte strategies struggle to balance these aspects. Here, we present a concentration-function coupled electrolyte strategy that enables the independent yet synergistic regulation of bulk and interfacial behaviors of Zn.

[Comparison between sinking and floating fresh Rehmanniae Radix samples by UHPLC-Q-Orbitrap HRMS, fingerprinting, and chemometrics].

This study aims to explore the scientific connotation of sinking Rehmanniae Radix has the best quality and compare the quality between floating and sinking fresh Rehmanniae Radix samples. Ultra-performance liquid chromatography tandem quadrupole electrostatic field Orbitrap high-resolution mass spectrometry(UHPLC-Q-Orbitrap HRMS) was employed to detect the chemical components in floating and sinking fresh Rehmanniae Radix samples. The fingerprint of fresh Rehmanniae Radix was established by high performance liquid chromatography(HPLC), and four index components were determined simultaneously.

[Research progress in application characteristics of plant-derived exosome-like nanovesicles in intestinal diseases].

Inflammatory bowel disease is a chronic, idiopathic, and recurrent gastrointestinal disorder with an unclear etiology and uncertain pathogenesis. Traditional treatment strategies rely on frequent administration of high doses of medication to reduce inflammation, whereas these approaches have limitations and may induce potential complications. Therefore, finding more effective and safe therapeutic drugs and methods is particularly important.

Comparative benchmarking of single-cell clustering algorithms for transcriptomic and proteomic data.

Background: Differences in data distribution, feature dimensions, and quality between different single-cell modalities pose challenges for clustering. Although clustering algorithms have been developed for single-cell transcriptomic or proteomic data, their performance across different omics data types and integration scenarios remains poorly investigated, which limits the selection of methods and future method development.

Results: In this study, we conduct a systematic and comparative benchmark analysis of 28 computational algorithms on 10 paired transcriptomic and proteomic datasets, evaluating their performance across various metrics in terms of clustering, peak memory, and running time.

Dual-signal amplified aptasensor based on PtPdAu mesoporous nanozymes with DNA walker-CHA for ultrasensitive DON detection.

Deoxynivalenol (DON), a prevalent trichothecene mycotoxin in cereals, poses severe threats to human health and agricultural sustainability. Conventional detection methods face limitations in sensitivity and operational complexity for on-site applications. Herein, we develop an electrochemical aptasensor integrating dual-signal amplification strategies: Nb.

Mechanisms of podophyllotoxin-induced enterotoxicity: A multi-omics integration of gut microbiota, short-chain fatty acids, and inflammatory mediators.

Podophyllotoxin (PPT), a lignan extracted from the roots and stems of Podophyllum species, exhibits significant enterotoxicity that limits its clinical application. However, its underlying mechanisms remain unclear. This study aimed to elucidate the mechanisms underlying PPT-induced enterotoxicity.

Quercetin alleviated cadmium-induced oxidative stress, endoplasmic reticulum stress, and autophagy in chicken kidneys.

Cadmium (Cd) is a toxic carcinogen that severely damages many tissues and organs. Quercetin (Que) is a flavonol known for its excellent antioxidant properties. Forty-eight 1-day-old male Hyland egg-laying chickens were assigned to the following treatments: control group; 150 mg/kg Cd group; 250 mg/kg Que group; and 150 mg/kg Cd + 250 mg/kg Que group.

Resveratrol-pretreated ADSC-CM alleviates liver oxidative damage in mice by mediating the Sirt1/Nrf2 axis.

Chronic liver injury is a prevalent pathological condition. Adipose-derived stem cell-conditioned medium (CM) has demonstrated efficacy in attenuating liver injury. Resveratrol (Res) pretreatment enhances the therapeutic effect of ADSCs, but whether it can enhance the protective effect of CM against CCl-induced liver injury is unknown.

Evidence-based practices for prevention and management of medical device alarm fatigue in anesthesia professionals.

Objective: To assess efficacy and implement the best available evidence for managing and preventing alarm fatigue in a healthcare context.

Methods: Four phases of evidence acquisition, status review, evidence application, and effect evaluation were used to apply evidence-based practice to medical care in the PACU between January and June 2024. Prior to and following the application of evidence, the occurrence of unfavorable outcomes pertaining to the management of surgical patient monitors and anesthesia alarm reports, the degree of evidence-based organizational culture, the implementation rate of review indicators, and the associated knowledge level of alarm fatigue prevention and management were all compared.

Arousal Threshold Score: A New Indicator for Examining the Relationship Between Obstructive Sleep Apnea and Overlap Syndrome - A Retrospective Study.

Objective: A low arousal threshold (AT) appears to contribute to obstructive sleep apnea (OSA) pathogenesis. However, the role of low AT in OSA and overlap syndrome (OVS) is still unclear. This study is aimed to investigate the value of the AT score, a new method for qualifying AT, for examining the relationship between OSA and OVS, including chronic obstructive pulmonary disease (COPD) and asthma.

Construction and optimization of a nisin-controlled expression vector using a pre-screened strong promoter in .

The nisin-controlled gene expression (NICE) system is an efficient and promising gene expression system for . To enhance the expression efficiency of the NICE system in ATCC19258, an inducible expression vector, pNZ8148-PnisA-gfp-PnisR-nisR-nisK, containing the regulatory element NisR/K and the promoter PnisR, was first constructed using the basic plasmid pNZ8148. Green fluorescent protein (GFP), as the reporter protein, was cloned downstream of PnisA in the vector pNZ8148 to detect protein expression.

Developing and Applying RNA Empirical Models With Secondary Structure Insights for Orthoptera Phylogenetics.

The evolutionary patterns exhibited by the ring and stem regions of the RNA secondary structure are distinct. Incorporating RNA secondary structure information into evolutionary models can improve the reliability of phylogenetic trees constructed using RNA sequences. However, empirically derived RNA evolutionary models remain scarce.

The putative AraC-type transcriptional regulator STM1082 facilitates pathogenicity through modulation of intestinal invasion and intracellular replication.

is a food-borne zoonotic pathogen that causes human gastroenteritis and potentially fatal systemic infections. The invasion of intestinal epithelial cells and the survival and replication within host macrophages are essential for the intestinal and systemic infections caused by , respectively. To precisely regulate the expression of virulence-related genes, relies on a series of regulatory proteins.

Fused-ring isomerization dopant-free hole transport materials for high-performance inorganic perovskite solar cells.

We synthesized two linear dopant-free hole transport materials (HTMs), denoted as NT and iNT, by coupling methoxytriphenylamine donor unit with either naphtho[1,2-:5,6-']bis[1,2,5]thiadiazole or its isomeric counterparts naphtho[1,2-:5,6-']bis([1,2,3]thiadiazole) as acceptor units. The fused-ring isomerization structure of iNT endows it with a substantial dipole moment and well-aligned energy levels, which are highly favorable for efficient free-charge extraction. Compared to devices based on NT, CsPbI inorganic perovskite solar cells (IPSCs) employing iNT exhibited significantly enhanced performance, achieving a power conversion efficiency (PCE) of 18.

Response of Anammox Process to Chloroacetonitrile and Accelerated Recovery With Pulse Electric Field.

Chloroacetonitrile poses significant challenges to anaerobic ammonium oxidation (anammox) process due to its toxicity. This study evaluated the effects of chloroacetonitrile on anammox and further explored the potential of a 1.5 V and 1000 Hz pulsed electric field (PEF) in mitigating prolonged chloroacetonitrile stress.

Research Progress on Histone Lactylation Modification in Ischemic Stroke: Mechanisms and Therapeutic Potential.

Ischemic stroke (IS) is a leading cause of death and disability worldwide, with a complex pathogenesis involving neuroinflammation, oxidative stress, and disruption of the blood-brain barrier. Recent research has brought histone lactylation into focus as a novel epigenetic modification that plays a crucial role in IS. Under cerebral ischemia and hypoxia, lactate levels rise, providing an alternative energy source to support ischemic brain regions.

The Application of Metal Nanoparticles in Oral Inflammatory Diseases.

Oral inflammatory diseases pose a significant global health challenge due to their high incidence and risk of systemic complications. Conventional treatment methods are limited by issues such as antibiotic resistance, poor drug delivery efficiency, and immunosuppressive side effects, which create an urgent need for innovative therapeutic approaches. Metal nanoparticles (MNPs), as promising candidates, have unique antibacterial and immune-regulating properties, which arise from their nanoscale size, excellent targeted penetration, and diverse biological activities.