Multifaceted Role of RIMBP2 in Promoting Hearing in Murine Cochlear Hair Cells.

The mammalian cochlea relies on outer and inner hair cells (OHCs/IHCs) for sound amplification and signal transmission. Rab3-interacting molecular binding protein 2 (RIMBP2), expressed in receptor cells and neurons at synaptic active zones, remains poorly characterized in hearing. We therefore generated a Rimbp2 knockout (KO) mouse model (Rimbp2), which exhibited severe hearing loss with elevated thresholds, prolonged latencies, and reduced amplitudes in auditory brainstem response Wave I.

Factors Influencing Prolonged Hospital Stay in Surgically Treated Children and Adolescents with Left-to-Right Shunt Congenital Heart Disease: Evidence From a Hospital in Southern China.

Background: Congenital heart disease(CHD) is a congenital malformation caused by abnormal development of the heart and large blood vessels, and left-to-right shunt CHD is a relatively common type. Surgical treatment has the problem of prolonged hospital stay in CHD patients. It is of great significance to explore the influencing factors of prolonged hospital stay after surgery therapy for children and adolescents with left-to-right shunt CHD.

Per- and Polyfluoroalkyl Substances (PFASs) in Market Meats from Guangzhou, China: Age-Specific Dietary Exposure and Threshold Exceedance Risks.

Per- and polyfluoroalkyl substances (PFASs), known for their environmental persistence and adverse health effects, pose an elevated risk to vulnerable populations through dietary exposure. Yet, there is limited research on PFAS concentrations in market basket meats and offal from Guangzhou, China. We examined the distribution of 16 PFAS in 166 market basket samples (beef, pork, and offal (pork liver)) from Guangzhou.

Biochar and hydroxyapatite enhance both phytoextraction and phytostabilization of a heavily Cd-polluted soil using sweet sorghum.

Sweet sorghum has a high tolerance to toxic metals, but its response to soil amendments in Cd-polluted soils remains underexplored. Here, we compared the effects of biochar and hydroxyapatite (HAP) at different doses (0, 0.5%, and 1%, w/w) on the growth, mineral nutrition, stress tolerance, and phytoremediation efficiency of sweet sorghum grown in an agricultural soil heavily polluted by Cd (20.

Metabolomics analysis of mechanism of improving quality of Schisandrae chinensis fructus by NO combining with high-temperature stress.

The fruits of Schisandra chinensis (Turcz.) Baill. (Schisandrae chinensis fructus) are a well-known herbal medicine, known for its hepatoprotective, antidepressant, antioxidant, and sedative-hypnotic properties.

Refractive Index-Corrected Light-Sheet Microscopy for Macro-View Cardiovascular Imaging.

Light-sheet fluorescence microscopy (LSFM) enables rapid data acquisition with minimal phototoxicity, while optical clearing reduces light scattering by matching sample and imaging medium refractive indices (RIs). Emerging clearing methods extend from cells and organoids to entire organs and organisms, prompting macro-view LSFM microscopes with macro-objectives for low-magnification imaging of larger specimens at adequate resolution. In cardiovascular studies, multiple organs often require imaging, yet clearing protocols optimized for different organs alter the sample RIs inconsistently.

Sintilimab and anlotinib with gemcitabine plus cisplatin in advanced biliary tract cancer: SAGC a randomized phase 2 trial.

Biliary tract cancer (BTC) has a poor prognosis with limited treatment options. This phase 2 trial randomized 80 patients with unresectable/metastatic BTC 1:1 to sintilimab, anlotinib, and gemcitabine/cisplatin (SAGC) or chemotherapy alone (GC). At 13.

Extracellular vesicles in reproductive biology and disorders: a comprehensive review.

Extracellular vesicles (EVs) facilitate intercellular communication and the conveyance of bioactive substances, including proteins, lipids, and nucleic acids. They play a significant role in various reproductive biological processes, including gametogenesis, fertilization, early embryo development, and implantation. Dysfunctional EV activity is associated with various reproductive diseases, such as polycystic ovary syndrome (PCOS), endometriosis, male infertility, and recurrent pregnancy loss (RPL).

Development of a convenient and rapid screening zebrafish model to investigate lethal effects and immunocompromised states of uremic toxins.

Background: Uremia is characterized by high mortality and immune dysfunction owing to the accumulation of uremic toxins. Traditional rodent models are complex and time consuming. This study aimed to develop a simple and rapid zebrafish model for investigating the toxicological effects of uremic toxins.

Identification and validation a novel kinase-related gene signature for predicting prognosis and responsiveness to immunotherapy in hepatocellular carcinoma.

Liver cancer research highlights the kinome's critical role in disease initiation and progression. However, comprehensive data analysis on the kinome's impact on hepatocellular carcinoma (HCC) prognosis is limited. We used the TCGA-LIHC mRNA expression profiles, analyzing them with various R packages.

Band alignment and optoelectronic characteristics of blue phosphorene/SbN van der Waals heterostructures.

van der Waals heterostructures are promising for electronic and optoelectronic devices. Here, we theoretically construct the blue phosphorene/SbN van der Waals heterostructure to investigate the band alignment, carrier mobility and optical properties, considering the influence of interlayer distance, biaxial strain and external electric field. The results show that the structure possesses the characteristics of a staggered type-II band alignment, which promotes electron and hole distribution inside different monolayers.

Dual Role of Natural Killer Cells in Early Pregnancy: Immunopathological Implications and Therapeutic Potential in Recurrent Spontaneous Abortion and Recurrent Implantation Failure.

Natural killer (NK) cells are critical regulators of immune processes during early pregnancy, playing a key role in maintaining maternal-foetal immune tolerance and supporting successful implantation. In particular, uterine NK cells, a specialised subset of NK cells, facilitate trophoblast invasion, spiral artery remodelling and placental establishment. Dysregulation of NK cell activity, however, has been implicated in pregnancy complications, notably recurrent spontaneous abortion (RSA) and recurrent implantation failure (RIF).

Antimicrobial Resistance and Genomic Characterization of Isolated from Pigeons in China.

Salmonellosis is one of the important bacterial infectious diseases affecting the health of pigeons. Heretofore, the epidemiological characteristics of in pigeon populations in China remain largely unclear. The present study investigated the antimicrobial resistance and genomic characteristics of isolates in pigeons in different regions of China from 2022 to 2023.

Genome-Based Molecular Diversity of Extended-Spectrum -Lactamase-Producing From Pigeons in China.

Extended-spectrum -lactamase-producing (ESBL-EC) strains present a significant menace to the well-being of both animals and humans. However, limited information is available regarding their profiles in pigeons. Using a combination of whole genome sequencing, drug susceptibility testing, and bioinformatics analysis, we examined the genomic features and epidemiology of 95 ESBL-EC strains (41 racing and 54 meat pigeons) that were isolated from 11 Chinese cities.

GPR83 protects cochlear hair cells against ibrutinib-induced hearing loss through AKT signaling pathways.

Introduction: Ibrutinib, widely used in leukemia treatment, has been implicated in sensorineural hearing loss; however, its underlying mechanisms remain unclear.

Methods: This study investigated the impact of ibrutinib on hearing using HEI-OC1 cells, cochlear explants and C57BL/6 J mice. We used RNA-sequences analysis to investigate the potential mechanisms of ibrutinib-induced ototoxicity.

Persistent Fibrosis in Heart Failure With a Reduced Ejection Fraction Linked to Phenotypic Differences in Human Cardiac Fibroblast Populations.

Background: Myocardial fibrosis is prevalent in cardiomyopathies that result in heart failure with reduced ejection fraction. Heart failure with reduced ejection fraction treated with a left ventricular assist device (LVAD) yields hemodynamic unloading and may provide partial cardiomyocyte recovery, but contemporary studies reveal no consistent reductions in fibrosis. This study tested the hypothesis that, despite normalization of hemodynamic overload by LVAD, fibrosis and fibroblast activation persist resulting in sustained increases in myocardial stiffness.

Neoadjuvant chemotherapy combined with bevacizumab for resectable colorectal liver metastasis with risk factors for recurrence: a multicenter real-world study.

Background: Currently, guidelines prohibit the addition of targeted drugs in neoadjuvant chemotherapy (NAC) for initially resectable colorectal liver metastasis (CRLM).

Objective: Nevertheless, efficacy data of NAC combined with bevacizumab (Bev) for initially resectable CRLM with risk factors for recurrence (RFR) are lacking.

Designs: We conducted a multicenter real-world cohort study to retrospectively analyze the efficacy and feasibility of NAC combined with Bev for CRLM with RFR.

Predictive evidence for the impact of personality styles on depression and functioning in two bipolar disorder cohorts.

Predictors for course of illness in bipolar disorder (BD) with replicable effect are difficult to identify. Potential predictors of outcomes for BD that could inform practice include personality traits, particularly Neuroticism. However, models typically fail to consider the joint effect of multiple personality traits.

Lycium barbarum Glycopeptide Alleviates Neomycin-Induced Ototoxicity by Inhibiting Tryptophan Hydroxylase-Mediated Serotonin Biosynthesis.

Aminoglycoside antibiotic-induced sensorineural hearing loss (SNHL) is a common sensory disorder that requires the development of prophylactic and therapeutic interventions. Lycium barbarum glycopeptide (LBGP) is a peptidoglycan isolated and purified from Lycium barbarum polysaccharides that exhibit significant anti-inflammatory, antioxidant, and neuroprotective effects, but the role of LBGP in aminoglycoside-induced SNHL has not been well investigated. Here it is shown that LBGP can protect against neomycin-induced hearing impairment and alleviate oxidative stress in a neomycin-induced SNHL mouse model.

Assessing structure - Function relationships in non-neovascular age-related macular degeneration.

Age-related macular degeneration (AMD), a neurodegenerative disease, is the leading cause of visual impairment in industrialized countries. Challenges in defining structural/functional relationships at various stages of disease especially with non-neovascular AMD, have slowed therapeutic development. Development of such sensitive and specific markers associated with AMD progression could provide the basis necessary for future regulatory outcome variables that will be useful in assessing new, innovative AMD therapies.

Complete chloroplast genome data of Rehb, an important medicinal plant species.

Rehb (commonly known as short petal golden lotus) Ic. Pl. Crit.

Chinese Association of Ultrasound in Medicine and Engineering, Superficial Organs and Peripheral Vessels Committee Expert Consensus on Selected Common Clinical Issues in Parathyroid Ultrasound (2024 Edition).

Parathyroid ultrasound is widely used in clinical practice and plays a crucial role in the diagnosis and treatment of parathyroid diseases. Nevertheless, ultrasound physicians frequently encounter a number of challenges and doubts in their professional practice. For this reason, Superficial Organs and Peripheral Vessels Committee of Chinese Association of Ultrasound in Medicine and Engineering has formulated the expert consensus on certain common clinical problems of parathyroid ultrasound based on the current research progress and clinical experience, in order to guide the clinical practice.

Protein Interaction with Zwitterionic Spherical Polyelectrolyte Brushes as Observed by Small Angle X-ray Scattering.

Understanding how zwitterionic spherical polyelectrolyte brushes (SPB) fulfill their antifouling functions requires knowledge of their interactions with exogenous nanoparticles, such as proteins. In this study, zwitterionic SPB were synthesized by grafting 3-[(2-(methacryloyloxy)ethyl)dimethylammonio]propanoate (CBMA) and [2-(methacryloyloxy)ethyl]dimethyl-(3-sulfopropyl) ammonium hydroxide (SBMA) onto a polystyrene core via photoemulsion polymerization. Small-angle X-ray scattering was employed to elucidate the interactions and protein adsorption behaviors of the zwitterionic SPB and proteins.

RONIN/HCF1-TFEB Axis Protects Against D-Galactose-Induced Cochlear Hair Cell Senescence Through Autophagy Activation.

Age-related hearing loss is characterized by senescent inner ear hair cells (HCs) and reduced autophagy. Despite the improved understanding of these processes, detailed molecular mechanisms underlying cochlear HC senescence remain unclear. Transcription Factor EB (TFEB), a key regulator of genes associated with autophagy and lysosomes, crucially affects aging-related illnesses.

Nuclear Magnetic Resonance Screening Approach for Discovery of 5-Hydroxymethylfurfural Derivatives from Marine Fungus Fusarium oxysporum SP7.

By tracing the characteristic proton and carbon nuclear magnetic resonance signals of the furan ring and aldehyde group, four novel 5-hydroxymethylfurfural derivatives, fusaroxysporins A-D (1-4), together with 10 known compounds were obtained from the marine fungus Fusarium oxysporum SP7. Their structures were elucidated by comprehensive spectroscopic analysis. The possible biogenic synthesis pathways of these compounds were speculated.