Machine learning based CAGIB score predicts in-hospital mortality of cirrhotic patients with acute gastrointestinal bleeding.

Acute gastrointestinal bleeding (AGIB) is a potentially lethal complication in cirrhosis. In this prospective international multi-center study, the performance of CAGIB score for predicting the risk of in-hospital death in 2467 cirrhotic patients with AGIB was validated. Machine learning (ML) models were established based on CAGIB components, and their area under curves (AUCs) were calculated and compared.

Protein-Binding Patterns Drive the Transport of Mercury in Plasma and Red Blood Cells in Rats.

The fate of metals within biological systems is determined by their associated binding partners, specifically protein ligands. However, the mechanisms by which these protein targets regulate the transport of metal pollutants remain unclear. Here, protein-binding patterns were identified as drivers of the transport of mercuric compounds (methylmercury and inorganic mercury) in the bloodstream.

The advantages in crop yields and carbon footprints of maize-soybean relay strip intercropping.

Intercropping can improve nutrient utilization, but the mechanisms of greenhouse gas (GHG) emissions, energy use, and carbon footprint in rain-fed cereal-legume intercropping remain unclear. Hence, a two-year field trial was conducted to evaluate cropping systems' productivity, carbon footprint, energy inputs, and outputs. The cropping systems include monocropping maize (M) and soybean (S), maize-soybean relay strip intercropping (IMS), and maize-soybean alternate row relay intercropping (CMS).

Ancient allopolyploidy and specific subgenomic evolution drove the radiation of poplars and willows.

Allopolyploidy involves the fusion of genomes from different lineages through hybridization and chromosome doubling. However, detecting early allopolyploidy events and understanding the specific subgenomic evolution that contributes to the origin of adaptive innovations for species radiation can be challenging. Here, we present genomes representing all three subfamilies of Salicaceae, a woody model clade, and collect epigenetic and transcriptomic samples.

Circulating cytokine profiling and clustering identify biomarker predicting efficacy of ICI in combination with chemotherapy.

The combination of chemotherapy can enhance the efficacy of immune checkpoint inhibitors (ICIs), but requires precise patient stratification and biomarker screening. Cytokines influence immunotherapy outcomes, and multiplex cytokine profiling aids in identifying predictive biomarkers for ICIs. We analyzed 1331 plasma samples (1025 untreated pan-cancer patients and 306 healthy controls), including 238 receiving ICIs plus chemotherapy.

Pomelo Peel and Soybean Meal Fermented Compound as Feedstuff for Large Yellow Croaker (): A Study on Growth and Intestinal Health.

Pomelo peel is a main by-product of pomelo consumption, while most of the by-products are discarded as wastes with environmental threats. Indeed, it contains many nutrients and bioactive ingredients, which makes it a promising source of aquatic feedstuff. In this study, a 56-day feeding trial was set out to assess the dietary application of pomelo peel and soybean meal (SBM) (1:4 ratio) fermented compound (PSFC) in large yellow croaker ().

Prevalence of Polyhalogenated Carbazole in an Anthropogenically Impacted Soil Groundwater System.

It has been proven that highly halogenated conditions could favor the generation of PHCZs in laboratory experiments, which has not been confirmed in the environment. In this study, carbazoles (CZs) and eight polyhalogenated carbazoles (PHCZs) were investigated in the groundwater-surface soil system along the coast of Laizhou Bay, around the main halogen producing areas, covering chemical enterprises, agriculture, aquaculture, and salt fields. The concentrations of PHCZs in surface soil and groundwater samples range from 0.

A Wireless Cytosensor Based on a Bionic Dandelion Isothermal Amplification System for Ultrasensitive Detection of Circulating Tumor Cells.

Accurate detection of circulating tumor cells (CTCs) remains a critical challenge in clinical oncology due to limitations in sensitivity, cost-effectiveness, and operational complexity. In this study, a wireless cytosensor is developed, leveraging a bionic dandelion isothermal amplification system (BDIAS) and wireless lateral flow immunoassay (LFIA) technology. The BDIAS, composed of a hexapod DNAwalker, nonlinear DNA self-assembly technology and an asymmetric carrier, and AuFe Janus nanoparticles (AuFe JNPs) with high signal probe loading efficiency, exhibits remarkable amplification efficiency.

Dodecylmethylaminoethyl methacrylate inhibits the growth of Candida albicans and Enterococcus faecalis biofilm and the formation of osteoclast.

Objectives: This study aimed to evaluate the antimicrobial ability on the dual-species biofilm formed by C. albicans and E. faecalis and osteoclysis inhibition of DMAEM.

Integration of Metabolomics, Lipidomics, and Machine Learning for Developing a Biomarker Panel to Distinguish the Severity of Metabolic-Associated Fatty Liver Disease.

Metabolic-associated fatty liver disease (MAFLD), a global health challenge linked to metabolic syndrome, requires accurate severity stratification for clinical management. Current invasive diagnostic methods limit practical implementation. This study integrated multi-omics and machine learning to establish a non-invasive biomarker panel for the early stage of MAFLD assessment.

HIPP26L-NF-YC9-SRMT module regulates drought response in poplar.

Drought severely limits the growth and development of perennial trees, which activate the abscisic acid (ABA) signaling pathway to cope with this condition. Despite its importance, the transcriptional regulatory mechanisms associated with ABA in poplars remain poorly understood. In this study, we identified HIPP26-like (HIPP26L), a heavy-metal-associated isoprenylated plant protein gene in poplar, which localizes to the cytomembrane, cytoplasm, and nucleus under normal conditions but accumulates in the nucleus in response to ABA and mannitol treatment.

Wnt/β-catenin Pathway Activation Reverses Tau Hyperphosphorylation and β-Amyloid Accumulation Induced by Combined Manganese and Iron Exposure in PC12 Cells.

Manganese and iron are essential trace elements involved in critical neuronal processes; however, excessive exposure to these metals is a significant risk factor for Alzheimer's disease (AD). While most previous studies have focused on single-metal neurotoxicity, the mechanisms underlying combined manganese and iron exposure remain unclear. In this study, we investigated the effects of manganese and iron exposure, both individually and in combination, on tau hyperphosphorylation, β-amyloid (Aβ) accumulation (particularly Aβ), apoptosis, and the involvement of the Wnt/β-catenin signaling pathway in PC12 cells.

Genome-wide identification of the HD-ZIP IV gene family in and its association with glandular hair development.

Background: HD-ZIP IV transcription factors are a plant-specific subgroup of the homeodomain-leucine zipper (HD-ZIP) family, known to regulate epidermal cell differentiation. While their functions have been studied in many plant species, their roles in (patchouli) remain largely unexplored.

Methods: We conducted a comprehensive genome-wide identification and analysis of genes in patchouli.

Functionalization-dependent toxicity of MIL-101 metal-organic frameworks to phytoplankton Chlamydomonas reinhardtii.

The MIL-101 series metal-organic frameworks have attracted significant attention in water remediation, leading to their inevitable release into the aquatic environments. However, research on the toxic effects of MIL-101 on aquatic organisms is in its infancy, and the responses of freshwater algae to MIL-101 and its amino-functionalized counterpart, MIL-101-NH, are poorly understood. In this study, the freshwater green algae Chlamydomonas reinhardtii (C.

Tryptophan-Derived Metabolites and Glutamate Dynamics in Fatal Insulin Poisoning: Mendelian Randomization of Human Cohorts and Experimental Validation in Rat Models.

Insulin overdose may cause hypoglycemic encephalopathy. In this study, Mendelian randomization was employed to analyze changes in the serum metabolites of patients with hypoglycemic encephalopathy, and metabolomics analysis was conducted to detect differential metabolites in the serum of a rat model of hypoglycemic encephalopathy induced by insulin overdose. The results indicated an overall upward trend in the tryptophan metabolism pathway in patients with hypoglycemic encephalopathy and rats with hypoglycemic encephalopathy caused by insulin overdose, while serum glutamate levels declined.

MicroRNA Sequencing of Serum Exosomes Reveals miR205-5p as an Anti-Fibrogenic Factor Against Intestinal Fibrosis in Crohn's Disease.

More than half of Crohn's disease patients eventually develop intestinal strictures. Intestinal fibrosis is the excessive deposition of the extracellular matrix that obstructs intestinal movement. There is no approved medication to treat intestinal stricture.

Endoscopic variceal ligation combined with carvedilol versus endoscopic variceal ligation combined with propranolol for the treatment of oesophageal variceal bleeding in cirrhosis: study protocol for a multicentre, randomised controlled trial.

Introduction: Liver cirrhosis and its severe complication, oesophageal variceal bleeding (EVB), pose significant health risks. Standard treatment for EVB combines non-selective beta-blockers (NSBB) with endoscopic variceal ligation (EVL). Carvedilol, an NSBB with additional benefits, is preferred for compensated cirrhosis.

A deep learning model based on self-supervised learning for identifying subtypes of proliferative hepatocellular carcinoma from dynamic contrast-enhanced MRI.

Objectives: This study employs dynamic contrast-enhanced MRI (DCE-MRI) to noninvasively predict the proliferative subtype of hepatocellular carcinoma (HCC). This subtype is marked by high tumor proliferation and aggressive clinical behavior. We developed a deep learning prediction model that employs a dynamic radiomics workflow and self-supervised learning (SSL).

Effect of Sodium Para-Aminosalicylic Acid on Cuproptosis in PC12 Cells Exposed Manganese, Iron, and Copper.

Mixed exposure to trace metals (such as manganese, iron, and copper) may cause significant damage to the nervous system, potentially leading to neurodegenerative diseases. This study aimed to investigate the toxic effects of mixed exposure to manganese, iron, and copper on PC12 cells and its mechanisms, and to evaluate the therapeutic effects of sodium para-aminosalicylic acid (PAS-Na). We employed various experimental techniques, including 3-(4,5-dimethylthiazol- 2-yl)- 2,5-diphenyltetrazolium bromide assay (MTT assays), flow cytometry, and western blotting, to systematically analyze cell viability, redox homeostasis, copper ions concentration, and the expression of cuproptosis-related proteins.

ALKBH5-mediated m6A regulates the alternative splicing events of SRSF10 in ovarian cancer.

N6-methyladenosine (m6A) methylation was found to be involved in the tumorigenesis and development of ovarian cancer. Until now, it is not clear to identify the mechanism by m6A demethylase ALKBH5 affects RNA splicing in ovarian cancer. In this study, we examined ALKBH5 protein expression and m6A levels by immunohistochemistry and analyzed their correlation with clinical features and prognosis in patients with ovarian cancer.

Forensic identification of rape and murder case based on morphological features of hair: A case report.

In forensic identification, there are many cases of hair poisoning detection and analysis to assist forensic identification, with fewer reported cases using morphological features to assist forensic identification. Herein, we report the case of a 13-year-old girl who had been raped and killed. The body was abandoned in a snowy wilderness and was discovered 5 months later.

Trophic Transfer of Metal Nanoparticles in an Aquatic Food Chain Diminishes Their Toxicity Disparities.

Engineered nanoparticles (ENP) threaten aquatic ecosystems as they are increasingly released into the aquatic environment, which is the sink for most contaminants, throughout their life cycle. How the food web responds to ENPs or how they shape the food web remains unclear. Here, we report the environmental behavior and toxicity of four ENPs in an aquatic food chain, focusing on their uptake, depuration, and trophic transfer efficiency.

[Digital aesthetic-driven flowable resin injection restoration].

Achieving precise restoration of tooth function and personalized restoration of natural tooth esthetics has always been a significant challenge in direct restorative dentistry. The traditional direct restorative techniques are limited by the subjective operations of dentists, resulting in high technical sensitivity, long operation time, and unpredictable restoration results, making it difficult to meet patients' personalized demands for restoration outcomes. An innovative flowable resin injection technique was introduced in this study.

Near-Infrared Bioluminescence Assays for Protein-Protein Interactions and Cellular Membrane Fusion in Deep Tissues Using Split Akaluc Reconstitution.

Bioluminescence analysis using luciferase is an essential tool for studying biological processes in different cells. Split luciferase reconstitution is a technique that enables the analysis of biological events through the monitoring of protein-protein interactions. However, effective detection of cellular events remains challenging due to the limitation of light penetration into deep tissues and optical sensitivity.