Synergistic inhibition of autophagic flux and induction of apoptosis in cervical cancer cells by Mito-TEMPO and hyperthermia.

Background: Hyperthermia (HT), while a cancer treatment approach, isn't always effective alone. Therefore, identifying hyperthermia enhancers is crucial. We demonstrated that Mito-TEMPO ([2-[(1-Hydroxy-2,2,6,6-tetramethylpiperidin-4-yl) amino]-2-oxoethyl]-triphenylphosphanium, MT) acts as a potent thermosensitizer, promoting cell death in human cervical cancer (HeLa) cells.

RNF180 suppressed aggressiveness by degrading NOTCH1, TRIM24 and FOXC1, and chemoresistance by degrading ACC1 and ACLY in colorectal cancer.

Ring finger protein 180 (RNF180) is an E3 ubiquitin-protein ligase that promotes polyubiquitination and degradation. We analyzed the roles and molecular mechanisms of RNF180 during the tumorigenesis and progression of colorectal cancer (CRC) through bioinformatics analysis, in vivo and vitro experiments. RNF180 overexpression was observed in CRC, and positively associated with T, N and TNM staging or differentiation.

Seasonal blooms of in a temperate semi-enclosed bay: linking community succession to thermal and nutrient regimes.

is a key picocyanobacterium in coastal ecosystems, yet its seasonal bloom dynamics and environmental responses remain unclear in temperate coastal seas. Here, we integrated flow cytometry and gene analysis to investigate its bloom development and community succession in Laizhou Bay, based on 3 years of 10 seasonal surveys and a year-long monthly observation at a fixed station. blooms reached their peak in summer (up to 10 cells mL), particularly in the southern part of the bay, with high abundances in autumn as well.

Isochlorogenic acid C protects mouse embryonic fibroblast cells from oxidative damage via restoring SIRT3-SOD2 activity.

Isochlorogenic acid C (ICAC) has been used for the production of scented tea, medicinal tea, and soft drinks. The antiinflammatory, antibacterial, and antiviral properties of ICAC were demonstrated previously. However, it is unknown how ICAC regulates oxidative stress.

Macranthoside B Enhances Paclitaxel-induced Human Cervical Cancer Cell Apoptosis Through ROS-JNK Pathway.

Background/aim: This study aims to elucidate whether Macranthoside B (MB) intensifies paclitaxel (PTX)-induced apoptosis in human cervical adenocarcinoma HeLa cells as well as the underlying mechanisms.

Materials And Methods: The MTT assay was used to test the HeLa cell viability after MB and PTX co-treatment, and colony formation assay was utilized to evaluate cell proliferation. Reactive oxygen species (ROS) and mitochondrial membrane potential (MMP) were analyzed by flow cytometry.

River inflow and seawater intrusion shape distinct phytoplankton communities in jinghai bay, a coastal bay of the Yellow Sea.

Phytoplankton are key primary producers that shape the structure and function of estuarine ecosystems. However, the distinct effects of riverine inputs and seawater intrusion on phytoplankton communities remain unclear. This study investigated the seasonal dynamics of phytoplankton in Jinghai Bay during spring and summer 2023, focusing on the contrasting influences of river inflow and seawater intrusion.

Ecological risk assessment of heavy metals in seawater and sediments in Jinghai Bay: Evidence for ecosystem degradation in a coastal bay of the Yellow Sea.

Jinghai Bay is plagued by heavy metal (HM) pollution that is likely to increase in the coming decades along with social and demographic trends. However, relatively little is known about the pollution levels and ecological risks associated with HMs in seawater and sediments of the bay, limiting our ability to assess their impacts on local aquatic environment and to develop effective management strategies. We investigated the concentrations and spatial distribution of HMs (i.

Functionalization-dependent toxicity of MIL-101 metal-organic frameworks to phytoplankton Chlamydomonas reinhardtii.

The MIL-101 series metal-organic frameworks have attracted significant attention in water remediation, leading to their inevitable release into the aquatic environments. However, research on the toxic effects of MIL-101 on aquatic organisms is in its infancy, and the responses of freshwater algae to MIL-101 and its amino-functionalized counterpart, MIL-101-NH, are poorly understood. In this study, the freshwater green algae Chlamydomonas reinhardtii (C.

Exploring the therapeutic potential of 4,4'-dimethoxychalcone: Inducing apoptosis in cancer cells via ER stress and autophagy disruption.

In cancer therapeutics, natural flavonoid compounds are renowned for their diverse structures and broad biological activities, offering considerable opportunities for drug discovery. This study investigates the anticancer effects of the flavonoid 4,4'-dimethoxychalcone (DMC), focusing on its apoptotic mechanisms and therapeutic potential. Our findings reveal that DMC induces apoptosis by upregulating pro-apoptotic proteins (Bax, Bim, tBid) and downregulating anti-apoptotic proteins (Bcl-2, Mcl-1), with concurrent caspase-3 activation and PARP cleavage.

RNF180 weakened the lipid droplet formation and subsequent chemoresistance by destabilizing ACC1 and ACLY in esophageal cancer.

Objective: RNF180 (Ring finger protein 180) is an E3 ubiquitin-protein ligase that promotes polyubiquitination and proteasomal degradation. The study aimed to clarify the clinicopathological significances, signal pathways and molecular mechanisms of RNF180 expression in esophageal cancer.

Methods: We analyzed the clinicopathological significances and signal pathways of RNF180 expression in esophageal cancer (EC) through bioinformatics and pathological analysis.

Sedimentary heavy metal interactions with phytoplankton and zooplankton across the Bohai Sea.

Excessive heavy metals (HMs) pose significant threats to aquatic ecosystems and are known to affect plankton communities. However, relatively little is known about the complex interactions between plankton (i.e.

Molecularly Imprinted Electrochemical Sensor Based on MWCNTs/GQDs for the Detection of Sulfamethazine in Aquaculture Seawater.

In this work, a novel molecularly imprinted electrochemical sensor was proposed based on molecular imprinting technology for the detection of sulfamethazine. A glassy carbon electrode was modified with a composite material of carbon nanotubes and graphene quantum dots to effectively improve sensitivity. The molecularly imprinted electrochemical sensor was then prepared by electropolymerization using sulfamethazine as the template and o-phenylenediamine as the functional monomer on the modified electrode.

Fenvalerate exposure induces AKT/AMPK-dependent alterations in glucose metabolism in hepatoma cells.

Background: Fenvalerate (Fen) is a synthetic pyrethroid insecticide significantly associated with an increased risk of type 2 diabetes. Tumor cells exhibit a shift in glucose metabolism, known as the Warburg effect. Accordingly, we aimed to elucidate whether Fen interferes with insulin signaling and affects hepatoma cell metabolism.

Dkk3 inhibits the aggressiveness and mitigates chemoresistance through low lipid droplet formation in gastric cancer: A biomarker and gene therapy target.

Dkk3 is discovered through comparative studies of immortalized cells and their parental counterparts and might inhibit the aggressive phenotypes in malignant cells, demonstrating its tumor-suppressor activity. Here, we explored the clinicopathological significance of Dkk3 expression, the association between Dkk3 expression and immune microenvironment, and Dkk3-related signal pathways in gastric cancer (GC), and discovered the effects of Dkk3 on aggressiveness, chemoresistance and lipid droplet formation with molecular mechanisms investigated. Our data showed that plasma level of Dkk3 was low in GC and Dkk3 expression was negatively correlated with younger age, tumor size, depth of invasion, lymph node metastasis, clinicopathological stage and histological classification of GC.

Ecological risk assessment for BDE-47 in marine environment based on species sensitivity distribution method.

BDE-47 is the most abundant and toxic congener in the marine environment. Assessing the ecological risk of BDE-47 in seawater is of great importance to protect the marine species. However, the ecological risks of BDE-47 on marine species is still limited.

Implications of seawater characteristics on dissolved heavy metals in near-shore surface waters of the Yellow Sea.

To explore the response of dissolved heavy metals (HMs) along the Yellow Sea coast to seawater characteristics, we assessed the temporal and spatial variations as well as pollution levels of six dissolved HMs (Cu, Zn, Pb, Cd, Hg, and As) in surface seawater from 2021 to 2023. The results indicated that the average concentrations of HMs in the seawater were ranked as follows: Zn > Cu > As > Pb > Cd > Hg, with specific concentration ranges of 4.70-7.

Enhanced understanding of cinnamaldehyde's therapeutic potential in osteoarthritis through bioinformatics and mechanistic validation of its anti-apoptotic effect.

Introduction: Osteoarthritis (OA) is a globally prevalent joint disorder affecting approximately 240 million individuals worldwide. Cinnamaldehyde, known for its broad anti-inflammatory and anti-aging effects across various cell types, has not been investigated for its potential impact on apoptosis in OA chondrocytes.

Methods: To explore the effectiveness of cinnamaldehyde in mitigating knee osteoarthritis by reducing chondrocyte apoptosis, bioinformatics analysis was first conducted to identify apoptosis-associated differentially expressed genes (APDEGs).

Dynamics of phytoplankton assemblages determined by the HPLC-PIGMENTUM approach and the related environmental variables in the northern Chinese sea.

During the spring and summer of 2021, two investigations were conducted in the northern Chinese sea by using the high-performance liquid chromatography with the pigment-based chemotaxonomic tool PIGMENTUM. Results showed that the average chlorophyll a (Chl a) concentration during spring was significantly higher (p < 0.01) than that during summer.

Maize/soybean intercropping with nitrogen supply levels increases maize yield and nitrogen uptake by influencing the rhizosphere bacterial diversity of soil.

Introduction: Intercropping practices play a crucial role in enhancing and maintaining the biodiversity and resiliency of agroecosystems, as well as promoting stable and high crop yields. Yet the relationships between soil nitrogen, microbes, and yield in maize cultivated under maize/soybean intercropping systems remain unclear.

Methods: To fill that knowledge gap, here we collected maize rhizosphere soil at the staminate stage after 6 consecutive years of maize/soybean intercropping, to investigate how intercropping and nitrogen application rates affected nitrogen utilization by crops and soil microbial community composition and function.

Metabolic variation and oxidative stress responses of clams (Ruditapes philippinarum) perturbed by ofloxacin exposure.

Ofloxacin (OFL), one of the most widely used fluoroquinolone antibiotics, has been frequently detected in marine environments. Nonetheless, researchers are yet to focus on the effects of OFL on the benthos. In the present study, marine clams (Ruditapes philippinarum) were exposed to OFL (0.

The Antitumor and Sorafenib-resistant Reversal Effects of Ursolic Acid on Hepatocellular Carcinoma via Targeting ING5.

Inhibitor of growth 5 (ING5) has been reported to be involved in the malignant progression of cancers. Ursolic acid (UA) has shown remarkable antitumor effects. However, its antitumor mechanisms regarding of ING5 in hepatocellular carcinoma (HCC) remain unclear.

Composition and temporal dynamics of the phytoplankton community in Laizhou Bay revealed by microscopic observation and rbcL gene sequencing.

Laizhou Bay, a major breeding ground for economic marine organisms in the northern waters of China, is facing rapid environmental degradation. In this study, field surveys in this area were conducted in the spring, summer, and autumn of 2020. Microscopic observation and RuBisCO large subunit (rbcL) gene analysis were employed to understand the community structure and temporal dynamics of phytoplankton.

Effects of Long-Term Application of Nitrogen Fertilizer on Soil Acidification and Biological Properties in China: A Meta-Analysis.

Soil acidification is a global environmental problem with significant impacts on agricultural production, environmental protection, and ecosystem health. Soil acidification is widespread in China, affecting crop yields, agricultural product quality, and biodiversity. Since the 1980s, much work has been done on acidic soils in China, but it is controversial whether excessive nitrogen fertilizer application can lead to soil acidification mechanisms.

Estrogen treatment in combination with pyruvate kinase M2 inhibition precipitate significant cumulative antitumor effects in colorectal cancer.

It is well established that pyruvate kinase M2 (PKM2) activity contributes to metabolic reprogramming in various cancers, including colorectal cancer (CRC). Estrogen or 17β-estradiol (E2) signaling is also known to modulate glycolysis markers in cancer cells. However, whether the inhibition of PKM2 combined with E2 treatment could adversely affect glucose metabolism in CRC cells remains to be investigated.