Platelet-derived growth factor signaling in porcine testicular tubular somatic cells.

Mechanisms governing somatic cell interactions in the testis are not well defined. The platelet-derived growth factor (PDGF) pathway mediates epithelial-mesenchymal interactions and is involved in testicular morphogenesis in rodents. However, its roles in the testis of higher mammals remain largely unknown.

The Impact of Storage Time and Reheating Method on the Quality of a Precooked Lamb-Based Dish.

Ready-to-eat meat products face quality challenges during storage and reheating. This study aimed to (i) characterize the physicochemical/microbiological changes in stewed mutton during storage (4 °C/-18 °C, 0-28 days) and (ii) evaluate reheating methods (boiling vs. microwaving) on day-7 samples.

Proteolysis Targeting Chimera (PROTACs) in Cancer: From Molecular Mechanisms to Therapeutic Opportunities.

Cancer ranks as the second leading cause of mortality around the globe, only behind cardiovascular ailments, largely owing to challenges in early detection and a significant recurrence rate following standard treatments. Chemotherapy is effective in suppressing the swift proliferation of malignant cells; however, it also adversely impacts normal cells that replicate quickly. Consequently, it is essential to create alternative and safer treatment approaches that are more efficient.

A Sn-Ta-O-doped vertical graphene electrochemical sensor based on a machine learning prediction model for monitoring cadmium in beverages.

The growing diversity of beverages intensifies demand for on-site heavy metal detection to ensure safety. Multi-heteroatom co-doped graphene electrodes exhibit superior electrocatalytic activity and stability over traditional carbon-based electrodes, enabling their application in machine learning (ML)-assisted real-time Cd detection. An industrial manufacturing process combining physical and chemical vapor deposition was employed to fabricate novel tin(Sn)‑tantalum(Ta)‑oxygen(O)-doped vertical graphene (VG) (STO-VG) electrodes with uniform surface properties.

Machine learning based CAGIB score predicts in-hospital mortality of cirrhotic patients with acute gastrointestinal bleeding.

Acute gastrointestinal bleeding (AGIB) is a potentially lethal complication in cirrhosis. In this prospective international multi-center study, the performance of CAGIB score for predicting the risk of in-hospital death in 2467 cirrhotic patients with AGIB was validated. Machine learning (ML) models were established based on CAGIB components, and their area under curves (AUCs) were calculated and compared.

Real-world effectiveness of single-inhaler fluticasone furoate/umeclidinium bromide/vilanterol in Chinese patients with symptomatic chronic obstructive pulmonary disease (COPD): a post-marketing, prospective, multicenter, observational study.

Objective: To assess the effectiveness of single-inhaler fluticasone furoate/umeclidinium bromide/vilanterol (FF/UMEC/VI) in symptomatic chronic obstructive pulmonary disease (COPD) under real-world settings in China.

Methods: In this single-arm, prospective, observational study, participants received 12 weeks of FF/UMEC/VI treatment. The primary outcome was change from baseline (CFB) in COPD Assessment Test (CAT) score at Week 12.

Adopting a consensus on the dose of antenatal steroids used by obstetric and neonatal services in Singapore in anticipation of imminent preterm birth.

In Singapore, glucocorticoids are administered to pregnant women at risk of imminent preterm delivery. The primary desired benefit is maturation of the preterm fetal lung. The commonly used antenatal steroid regimen in Singapore, two doses of 12 mg dexamethasone phosphate at a 12-h interval, is not widely used in other jurisdictions.

A combination of recombinase polymerase amplification with CRISPR technology rapidly detects goose parvovirus with high accuracy and sensitivity.

Background: Goose parvovirus (GPV) poses a significant threat to the waterfowl industry, necessitating reliable detection methods. However, conventional techniques are often time-consuming, equipment-dependent, or lack sufficient sensitivity for detecting early-stage infection. In contrast, emerging CRISPR/Cas12a-based systems offer a promising alternative for rapid, sensitive, and on-site diagnostics.

The Assessment of Subglottic and Cervical Tracheal Stenosis Using a Novel Point-of-Care Ultrasound Method: Measurement of Tracheal Radius Curvature.

Background And Objective: The air way dimensions are important in the assessment, treatment and follow-up of patients with tracheal and subglottic stenosis (SGS). Non-invasive, non-radioactive methods to assess the degree of stenosis are needed. Point-of-care ultrasonography (POCUS) is an effective method for assessing the subglottic diameter and evaluating the abnormal airway.

Effects of dietary sodium butyrate supplementation on fat metabolism in lamb adipose and liver tissues.

Objective: Sodium butyrate (SB) is a potentially useful feed additive, however, its effects on lipid metabolism in adipose and liver tissues of lambs are still not fully explored. This study systematically examined the effects and underlying mechanisms of dietary SB supplementation on lipid metabolism in lamb adipose and liver tissues from an adipose-blood-liver-perspective.

Methods: Twelve 3-month-old male lambs (22.

Shen-Ying-Yang-Zhen formula promotes angiogenesis around hair follicles, alleviates oxidative stress, and inhibits hair follicle apoptosis through the VEGF/Akt/Caspase-9 signaling axis.

Background: Androgenic alopecia (AGA) represents the most predominant form of hair loss in clinical practice, yet currently approved pharmacological options (e.g., finasteride and minoxidil) face limitations in safety and long-term efficacy.

A magnetically actuated microcatheter with soft rotatable tip for enhanced endovascular access and treatment efficiency.

Endovascular interventions require fast access to affected regions, followed by effective treatment. Catheterizations are effective approaches for treating vascular diseases; however, they face challenges in accessibility, efficiency, and invasiveness in narrow, tortuous vascular systems. This study presents a submillimeter magnetically actuated soft rotatable-tipped microcatheter (MSRM) designed to access small blood vessels and provide efficient, minimally invasive therapeutic interventions for blood clot treatment.

G0S2: a potential target for NSCLC identified through prognostic models from multi-Omic analysis of regulatory T cell metabolic genes.

Non-small cell lung cancer (NSCLC) remains a leading cause of cancer-related mortality, with immunotherapy proving effective only in a subset of patients, highlighting the urgent need for improved biomarkers and therapeutic targets. This study explored regulatory T cell (Treg) metabolism-related genes in NSCLC and established a seven-gene prognostic signature (TIMP1, BIRC3, G0S2, PRKCB, PDE4B, CD52, and ACP5) through multi-omics analysis. The model stratified patients into high- and low-risk groups exhibiting distinct immune profiles and drug sensitivities.

Deciphering failure paths in lithium metal anodes by electrochemical curve fingerprints.

Understanding anode failure mechanisms in lithium metal batteries (LMBs) is crucial for their use in energy storage, as the anode directly affects battery stability and electrolyte selection. Unfortunately, post-mortem methods reveal failure outcomes but often miss dynamic progressions, obscuring cause-and-effect relationships in failure evolution. Leveraging domain knowledge informed machine learning and a 4-year dataset of over 18 000 cycles and 12 million data points, from cells cycled to failure, we uncovered a correlation between initial lithium plating/stripping behavior and subsequent anode changes, enabling the identification of early indicators for distinct failure types.

Dynamic alterations of circulating lymphocytes during the trajectory of Hantaan virus-induced hemorrhagic fever with renal syndrome.

Introduction: Hemorrhagic fever with renal syndrome (HFRS) is a zoonotic disease with high mortality. Almost 90% of global cases of HFRS are induced by Hantaan virus (HTNV) infection. Although lymphocyte dysfunction is a critical factor in HFRS progression, the specific immune dynamics of HTNV remain unexplored, and current analyses predominantly depend on single-time point sampling.

The impact of different Spartina alterniflora eradication strategies on the biological community.

The global invasive species, Spartina alterniflora has caused significant damage to coastal wetland ecosystems in China. This study evaluated the effectiveness and ecological impacts of two S. alterniflora management strategies in the Yangtze River estuary: Haloxyfop-R-methyl application and repeated mowing (once at the early flowering stage and once nine weeks later).

Adapting gas fermenting bacteria for light-driven domino valorization of CO.

The solar-driven valorization of CO to fuels and chemicals provides an exciting opportunity to develop a circular chemical industry, but the controlled production of multicarbon organics remains a major challenge. Here, we present an abiotic-biotic domino strategy that integrates a photocatalytic CO-to-syngas conversion system with evolved syngas-fermenting bacteria to enable the upcycling of CO into valuable C products, including acetate and ethanol. To optimize microbial syngas fermentation through an accessible and chemist-friendly platform, we employ adaptive laboratory evolution (ALE) of ().

RhNIRF1-mediated ubiquitination of RhNAC31 affects drought tolerance by regulating stress-related genes in Rosa hybrida.

Ubiquitin-mediated protein modification by E3 ligases is crucial for plant stress responses. Here, we demonstrate that the RING-type E3 ligase RhNIRF1 physically interacts with and ubiquitinates the NAC-domain transcription factor RhNAC31, establishing a regulatory module that governs drought tolerance in rose (Rosa hybrida). Silencing of RhNAC31 resulted in decreased dehydration tolerance, whereas its overexpression conferred enhanced photosynthetic capacity concomitant with reduced relative oxygen species accumulation.

Ivermectin induced early developmental damage to the nervous system of zebrafish larvae.

Ivermectin has good antiparasitic activity and is heavily used in animal husbandry. However, its extensive use puts pressure on the environment. This study focused on evaluating the safety of ivermectin on the nervous system of aquatic organisms.

From Infarction to Angiosarcoma: Unraveling the Mystery of Pericardial Hematoma.

Background: Primary cardiac angiosarcomas are extremely rare, highly aggressive tumors characterized by rapid progression and high metastatic capability.

Case Summary: We present a case with unexplained pericardial hematoma after repeated right coronary infarction, combined with multimodality imaging and histopathologic examination, finally diagnosed as angiosarcoma.

Discussion: There is no published case of angiosarcoma onset of pericardial hematoma after repeated myocardial infarction.

Safety and efficacy of inhalable ambroxol hydrochloride aerosol for adult patients with respiratory diseases: an open-label, single-arm, multicentre study.

Background: Airway mucus hypersecretion is a key pathophysiological feature in many respiratory diseases and could lead to airway obstruction and repeated infections, consequently accelerating disease progression, which impacts on pulmonary function and quality of life (QoL), highlighting the importance of mucolytic therapy targeting airway mucus hypersecretion.

Objectives: To investigate the safety and efficacy of inhalable ambroxol hydrochloride aerosol for adult patients with respiratory diseases.

Design: An open-label, single-arm, multicentre postmarketing surveillance study.

Integrated transcriptomics and metabolomics study on the biofilm formation of Haemophilus influenzae by the stimulation of amoxicillin-clavulanate at subinhibitory concentration.

Exposure to subinhibitory concentrations of β-lactam antibiotics has been shown to induce the biofilm formation of microorganisms, but the underlying mechanisms remain poorly understood. This study aims to explore the effect of different concentrations of amoxicillin-clavulanate, the most commonly used antibiotic in pediatrics, on the biofilm formation of Haemophilus influenza (H. influenzae) in vitro and to explore the underlying mechanisms.

From lab to field: Innovative RPA‒CRISPR/Cas12a platform for early short-beak and dwarfism syndrome virus nucleic acids detection.

Short beak and dwarfism syndrome virus (SBDSV) is the causative agent of short beak and dwarfism syndrome (SBDS), which is characterized by beak atrophy and dwarfism. SBDS has caused substantial economic losses for waterfowl husbandry industries. To address the urgent need for rapid and accurate SBDSV diagnostics, the study developed a dual-mode detection platform integrating recombinase-aided amplification (RPA) with CRISPR/Cas12a-mediated fluorescence and lateral flow strip readouts.

Construction of Fracture Liaison Service Scheme Under Medical Alliance Framework in China: A Modified Delphi Method Study.

Purpose: To devise an implementation blueprint for the fracture liaison service (FLS) model within the context of a medical consortium. The FLS is an integrated system designed to identify, register, assess, treat, and monitor patients with osteoporotic fractures. The FLS constitutes a structured intervention strategy to administer standardized care to osteoporotic fracture patients.