An Efficient and Explainable Ensemble-learning Framework for Early Lung Cancer Biomarkers Detection.

This study proposes an Efficient and Explainable Ensemble-learning framework (EEE-framework) designed for early detection of non-small cell lung cancer (NSCLC) biomarkers using gene expression data, specifically addressing challenges of interpretability in detection outcomes. The EEEframework comprises two core modules. The first module constructs an ensemble-learning model balancing detection accuracy and interpretability.

Poly(benzoxazine)-Based Gel Polymer Electrolytes for Lithium Metal Batteries With Ultralong Lifespans.

Gel polymer electrolyte (GPE) is a desirable candidate for high-safety lithium batteries but is still plagued by the dynamic fluctuations of liquid electrolyte components, which induce localized fluid aggregation or leakage, ultimately leading to performance instability or even degradation. Here, we develop a novel poly(benzoxazine-propylene-oxide)-based GPE, achieving superior electrochemical performance and high safety simultaneously. Through molecular architecture design, the strategic incorporation of long-chain propylene-oxide segments and amide functionalities into the benzoxazine backbone endows the polymer matrix with enhanced lithium-ion transport capability.

Geosynchronous orbit-based Earth radiation measurement system.

We have designed a set of Earth radiation measurement systems based on a segmented aperture method, which can be deployed in geosynchronous orbit. This system includes a four-channel radiometer and a nine-channel multispectral camera. The four-channel radiometer adopts a Cassegrain telescope system as the initial structure and combines segmented aperture technology to achieve simultaneous measurements of four Earth radiation bands (0.

Construction of an immune prediction model for osteosarcoma based on coagulation-related genes.

Objectives: The prognostic outcome of osteosarcoma, as the most common primary malignancy in children and adolescents, has not improved with the development of modern medical care, and the aim of this study was to investigate the role of the coagulation system in the diagnosis and development of osteosarcoma.

Methods: TRGET and GEO databases were used to acquire clinical information and matching RNA data from osteosarcoma patients. To find novel molecular groupings based on coagulation systems, shared clustering was used.

Altered expression of TEF gene in childhood constipation leads to a decrease in CD56 NK cells.

Objectives: This study used a quantitative bioinformatic analysis of public RNA sequencing databases to study key molecules mediating the occurrence of childhood constipation (CHC) and explore associated immune cell abnormalities and the role of natural killer (NK) cells.

Methods: Gene expression profiling datasets, including CHC (GSE36701), were obtained. Immune genes were downloaded from the MSigDB database, and 3,907 immune-related genes were obtained.

An indicator of antioxidant stress ability to evaluate neurofunctional changes in patients with acute ischemic stroke: preliminary findings.

Background: Acute ischemic stroke (AIS) ranks among the most prevalent cerebrovascular conditions. The clinical interest lies in utilizing oxidative stress (OS) markers and antioxidant system indicators for diagnosing and predicting stroke development. Our objective was to identify a precise marker of antioxidant stress ability to assess neurofunctional alterations in AIS patients.

The lipemia index: an efficient screening tool for identifying IgM paraprotein-mediated interference in the Beckman Coulter AU5800 uric acid assays.

Objectives: IgM paraprotein-mediated interference in Uric Acid (UA) measurement remains a significant challenge in clinical labs, potentially compromising hyperuricemia diagnosis and management. This study investigated the identification and elimination strategies for IgM interference in the Beckman Coulter AU5800 UA assays.

Methods: Serum samples without hemolysis, icterus, or lipemia from 196 patients with IgM paraproteinemia were analyzed for UA levels based on uricase-peroxidase enzymatic reaction and lipemia (LIP) index levels via transmission turbidimetry.

Artificial intelligence-based prediction of organ involvement in Sjogren's syndrome using labial gland biopsy whole-slide images.

Objectives: This study aimed to develop a deep learning-based model to predict the risk of high-risk extra-glandular organ involvement (HR-OI) in patients with Sjogren's syndrome (SS) using whole-slide images (WSI) from labial gland biopsies.

Methods: We collected WSI data from 221 SS patients. Pre-trained models, including ResNet50, InceptionV3, and EfficientNet-B5, were employed to extract image features.

Effect of ciprofol compared with propofol on hemodynamics in bronchoscope procedures during anesthetic induction: a randomized double-blind controlled study.

Objective: Propofol poses hemodynamic challenges and injection pain during anesthesia. This study compared the effects of propofol and ciprofol, a novel propofol analog, on hemodynamics in painless bronchoscopy during induction of general anesthesia.

Methods: A total of 250 patients underwent painless bronchoscopy anesthesia from October 2021 to June 2023.

Simultaneous Determination of Unbound Plasma Concentration of Methotrexate and 7-Hydroxymethotrexate in Children Patients Receiving High-Dose Methotrexate Therapy.

Background: High-dose methotrexate (HD-MTX) is seen as an effective therapy for acute lymphoblastic leukemia (ALL); however, it is extremely toxic. Monitoring the plasma concentrations of methotrexate (MTX) and its important metabolite, 7-hydroxy-methotrexate (7-OH-MTX), on a routine basis aids in dose modification of rescue medications and in avoiding toxicity. The pharmacologically active and toxic effects of drugs are due to the unbound portion, as most drugs are bound to plasma proteins to some degree.

Transcriptome Analysis Reveals Association of E-Class AmMADS-Box Genes with Petal Malformation in L.

Snapdragon () serves as a model system for dissecting floral morphogenesis mechanisms. Petal malformation in impacts ornamental value, but its genetic basis remains poorly understood. We compared transcriptomes of the wild-type (Am11) and a petal-malformed mutant (AmDP2) to identify 2303 differentially expressed genes (DEGs), including E-class MIKC-type MADS-box genes () and ().

A bibliometric analysis of electroencephalogram research in stroke: current trends and future directions.

Background: Electroencephalography (EEG) has become an indispensable tool in stroke research for real-time monitoring of neural activity, prognosis prediction, and rehabilitation support. In recent decades, EEG applications in stroke research have expanded, particularly in areas like brain-computer interfaces (BCI) and neurofeedback for motor recovery. However, a comprehensive analysis of research trends in this domain is currently unavailable.

Programmed intermittent bolus for erector spinae plane block versus intercostal nerve block in minimally invasive direct coronary artery bypass surgery: a randomized controlled trial.

Objective: Continuous intercostal nerve block (ICNB) has been shown to offer effective pain relief after minimally invasive direct coronary artery bypass (MIDCAB). The erector spinae plane block (ESPB) could represent a viable alternative approach. This study aimed to compare the analgesic effect of programmed intermittent bolus(PIB) for ESPB to ICNB in patients undergoing MIDCAB.

The protocatechuic acid catabolism regulator PcaV inhibits lincomycin biosynthesis either directly or indirectly through the control of the expression of other regulatory or metabolic genes.

Lincomycin, synthesized by Streptomyces lincolnensis, is extensively utilized in clinical settings to treat infections caused by Gram-positive pathogenic bacteria. Understanding the regulatory mechanism of lincomycin biosynthesis is crucial for improving its production. This study identified PcaV, a conserved regulator of protocatechuic acid catabolic genes, as a repressor of lincomycin biosynthesis.

Fermentation Optimization of Loonamycin Production by Marine Nocardiopsis flavescens.

The antitumor drug candidate, loonamycin, was isolated from Nocardiopsis flavescens NA01583 and exerts its antitumor activity by inhibiting topoisomerase I activity. However, the current production levels of loonamycin are insufficient for further research. To address this limitation, a liquid fermentation method was developed, optimizing both fermentation conditions and medium components.

Early Soybean-Nematode Interactions: Transcriptomic Responses of at the Preparasitic Stage and Metabolomic Profiling of Root Exudates.

Understanding the molecular and metabolic interplay between and soybean () root exudates is essential for unraveling plant-nematode interactions. This study investigates the transcriptomic responses of during the preparasitic stage and the metabolomic changes in soybean root exudates influenced by nematode activity. Transcriptomic analysis identified 846 differentially expressed genes in nematodes exposed to root exudates (S-Mi) compared with nematodes alone (Mi).

Unraveling the promotion for SO and HO resistance of transition metal-doped CeO-TiO catalysts in NH-SCR reaction: A DFT study.

SO and HO can cause severe deactivation of CeO-TiO catalysts for NH-SCR reaction. Transition metal doping is an effective method to improve the SO and HO resistance of CeO-TiO catalysts. In this study, five transition metals (W, Nb, Mo, Fe, Mn) were selected as doped elements for the CeO-TiO catalysts.

Manipulation of seed coat content for increasing oil content via modulating BnaMYB52 in Brassica napus.

Seed oil is synthesized in the embryo, which is surrounded by the seed coat. However, the genetic and molecular mechanisms of how seed coat development affects seed oil content (SOC) remains less studied. Through genome-wide association studies in Brassica napus accessions, we identify BnaA09.

The critical role of MLKL in hemorrhagic stroke and the therapeutic potential of its associated protein network.

Introduction: Mixed Lineage Kinase Domain-Like Protein (MLKL), as the executor of necroptosis and a critical factor in the inflammation, has been shown to be associated with the progression of hemorrhagic stroke. Studies identified MLKL is a promoting factor in this process, suggesting its potential as a therapeutic target to mitigate posthemorrhagic stroke damage. However, the mechanisms by which MLKL functions in the process of intracerebral hemorrhage (ICH)-induced damage remain unclear.

Positive hepatitis B surface antigen leads to a decrease in ovarian reserve in infertile patients receiving first in vitro fertilization treatment.

Background: This study assessed the impact of chronic hepatitis B virus (HBV) infection on ovarian reserve in women.

Methods: We analyzed data from 38,861 infertile women undergoing their first in vitro fertilization (IVF) treatment (2016-2022), including 1574 HBsAg-positive cases. A control group of 1574 HBsAg-negative women was matched by age and body mass index (BMI).

Probiotics alleviate painful diabetic neuropathy by modulating the microbiota-gut-nerve axis in rats.

Painful diabetic neuropathy (PDN) is one of the most common complications of diabetes. Recent studies suggested that gut microbiota dysbiosis contributes to the development of PDN, but underlying mechanisms remain elusive. In this study, we found decreased probiotics generating bacteria such as Lactobacillus and Bifidobacterium strains in the PDN rats.

MarR family regulator LcbR2 activates lincomycin biosynthesis in multiple ways.

Lincomycin, produced by the actinomycete Streptomyces lincolnensis, is highly effective against Gram-positive bacteria and protozoans, making it widely used in clinical settings. This study identified LcbR2, a MarR family transcriptional regulator, as an activator of lincomycin biosynthesis. Knocking out the lcbR2 gene reduced lincomycin production by 63.

Identification of pain-related long non-coding RNAs for pulpitis prediction.

Objectives: We investigated the recently generated RNA-sequencing dataset of pulpitis to identify the potential pain-related lncRNAs for pulpitis prediction.

Materials And Methods: Differential analysis was performed on the gene expression profile between normal and pulpitis samples to obtain pulpitis-related genes. The co-expressed gene modules were identified by weighted gene coexpression network analysis (WGCNA).

Peripheral blood lymphocyte subsets combined with fungal biomarkers in the diagnosis of invasive fungal infections complicated by sepsis.

Objective: To evaluate the lymphocyte subpopulations and the levels of (1, 3)-β-d-glucan (BDG) and galactomannan (GAL) in sepsis patients with secondary invasive fungal infections (IFIs), and to investigate the association between lymphocytes and IFIs.

Methods: A retrospective case-control study of 47 patients with sepsis complicated by IFIs, 50 sepsis patients, and 50 healthy controls was conducted. The correlation between lymphocyte subsets and BDG, GAL levels was analyzed using the Spearman correlation method, and the diagnostic efficacy of IFIs-related factors was assessed using the receiver operating characteristic curve (ROC) analysis.